FastQCFastQC Report
Fri 27 May 2016
SRR939860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413156
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295921.2262779530208574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202880.840724760438198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151620.6283058368377344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82820.34320201429165786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49780.20628587625499553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.1601637026367131No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36950.15311898609124316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36630.1517929218003312No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.15162716376396718No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.12361405561845153No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.10252134549113276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163750.038.7823871
GTATCAA268450.036.115751
CGTCACT4300.031.7257291
TATCAAC332600.029.234812
GTACATG235650.029.085341
ATCAACG338600.028.7741343
TCAACGC341200.028.5962354
TACATGG239000.028.2840062
CAACGCA343000.028.2547325
AACGCAG351600.027.72426
ACATGGG242800.026.893663
ATGGGAG58750.025.784945
CATGGGA175500.024.9563854
ACGCAGA393650.024.5236577
CGCAGAG393750.024.3018598
CATGGGG58800.024.1623254
GAGTACT208400.023.71389612-13
AAGACCG2853.6379788E-1223.110075
GCAGAGT394400.023.0809219
AGTACTT210800.022.5847112-13
Produced by FastQC (version 0.11.5)