FastQCFastQC Report
Fri 27 May 2016
SRR939860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413156
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315711.3082867415119455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237540.9843540989476022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182970.7582186978380179No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99360.4117429623281711No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.24855417552781503No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.19145053200041773No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.17769261498220587No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41600.17238835781855794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.15672422338216013No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33150.13737197263666334No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30550.1265977002730035No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG28000.1160306254547986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168000.038.528931
GTATCAA272550.035.4671861
GTACATG255000.028.733531
TACATGG259150.027.7200182
TATCAAC347500.027.7178842
ATCAACG349950.027.5028633
TCAACGC352400.027.2847354
CAACGCA357700.026.8671995
ACATGGG257200.026.742313
AACGCAG363900.026.4094456
CATGGGA187650.025.025834
TAAGGTG8300.024.5749265
ATGGGAT69050.023.4943965
GTCGGGA12750.023.4449462
GTAAGGT6900.023.3739264
ACGCAGA410400.023.3593527
CGCAGAG411100.023.2849628
CGTCACT5100.023.2622491
ATGGGAG61250.023.0787355
GAGTACT213900.022.57557712-13
Produced by FastQC (version 0.11.5)