##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413156 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38760361949248 31.0 31.0 34.0 30.0 34.0 2 31.587415815637282 31.0 31.0 34.0 30.0 34.0 3 31.602681716391317 31.0 31.0 34.0 30.0 34.0 4 35.43875033358805 37.0 35.0 37.0 33.0 37.0 5 35.20874158156373 37.0 35.0 37.0 32.0 37.0 6 35.266866294595125 37.0 35.0 37.0 32.0 37.0 7 35.16346353074563 37.0 35.0 37.0 32.0 37.0 8 35.13353094453902 37.0 35.0 37.0 32.0 37.0 9 36.655896676385616 39.0 35.0 39.0 32.0 39.0 10-11 36.537418011931265 38.5 35.0 39.0 32.0 39.0 12-13 36.4510133203158 38.0 35.0 39.0 32.0 39.0 14-15 37.31864620438961 39.0 36.0 41.0 32.0 41.0 16-17 37.30735062300158 39.0 36.0 41.0 32.0 41.0 18-19 37.42893248509421 39.0 36.0 41.0 32.0 41.0 20-21 37.41709508212482 39.0 36.0 41.0 32.0 41.0 22-23 37.2747064425176 39.0 36.0 41.0 31.5 41.0 24-25 37.099428507730124 39.0 36.0 40.5 31.0 41.0 26-27 36.77517388018015 39.0 35.0 40.0 30.5 41.0 28-29 36.52277225343077 38.0 35.0 40.0 30.0 41.0 30-31 36.13378600471747 38.0 35.0 40.0 30.0 41.0 32-33 35.8909718227914 38.0 34.5 40.0 30.0 41.0 34-35 35.63303470641766 38.0 34.0 40.0 29.0 41.0 36-37 35.46039211721082 38.0 34.0 40.0 28.0 41.0 38-39 35.249525932016 38.0 34.0 40.0 27.0 41.0 40-41 35.0569668931474 38.0 34.0 40.0 26.5 41.0 42-43 34.67513807644429 38.0 33.5 40.0 25.0 41.0 44-45 34.20740598618573 38.0 33.0 40.0 23.5 41.0 46-47 33.65139033696951 38.0 33.0 40.0 21.0 41.0 48-49 33.15196717493606 37.0 33.0 40.0 16.5 41.0 50-51 32.83867226155292 37.0 32.0 40.0 13.0 41.0 52-53 32.55950402709149 37.0 32.0 40.0 8.5 41.0 54-55 32.282594453073074 36.5 31.0 40.0 7.5 41.0 56-57 31.90836667832498 36.0 31.0 39.5 4.0 41.0 58-59 31.545916840850737 36.0 31.0 39.0 2.0 41.0 60-61 31.223902018767124 35.0 30.0 39.0 2.0 40.5 62-63 30.921224943600826 35.0 30.0 39.0 2.0 40.0 64-65 30.484572277962968 35.0 29.0 38.5 2.0 40.0 66-67 29.628577472819828 34.5 27.5 38.0 2.0 40.0 68-69 27.885300825972294 34.0 23.0 37.0 2.0 39.5 70-71 27.268192151688496 33.0 21.5 36.0 2.0 39.0 72-73 27.737456053400606 33.0 25.0 36.0 2.0 39.0 74-75 27.772532940265776 33.0 25.5 36.0 2.0 39.0 76-77 27.23107644097605 32.5 25.5 35.0 2.0 37.0 78-79 27.676447564931568 33.0 26.0 35.0 2.0 37.0 80-81 27.45059602445926 33.0 26.0 35.0 2.0 37.0 82-83 26.9943176073159 33.0 25.5 35.0 2.0 36.5 84-85 26.628674234073554 33.0 25.0 35.0 2.0 36.0 86-87 26.23888281569861 33.0 24.0 35.0 2.0 36.0 88-89 25.77305880763614 32.5 22.0 35.0 2.0 35.0 90-91 25.270994912885865 32.0 20.0 35.0 2.0 35.0 92-93 24.78885969245254 32.0 17.5 34.0 2.0 35.0 94-95 24.037405165683445 31.0 7.0 34.0 2.0 35.0 96-97 23.523142929839594 31.0 2.0 34.0 2.0 35.0 98-99 22.900484262103237 31.0 2.0 34.0 2.0 35.0 100-101 21.58763192267719 30.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3362591750921986 1101 2 0.3633989588601878 1101 3 0.36615893851269377 1101 4 0.24833252489008117 1101 5 0.25738289657670776 1101 6 0.2203220349185102 1101 7 0.27045762574932297 1101 8 0.2811356211467526 1101 9 0.49052171915322873 1101 10-11 0.4249608338639703 1101 12-13 0.36987969778926555 1101 14-15 0.35788003740746177 1101 16-17 0.4279892613226579 1101 18-19 0.4540684381618192 1101 20-21 0.47460351223558206 1101 22-23 0.3771358957260773 1101 24-25 0.4143576560424691 1101 26-27 0.26255838439654156 1101 28-29 0.3879841038603473 1101 30-31 0.13376060404009138 1101 32-33 0.24837724474493683 1101 34-35 0.23971862440902214 1101 36-37 0.5086047270945926 1101 38-39 0.5456196779851723 1101 40-41 0.45416295202948476 1101 42-43 0.3536339748150681 1101 44-45 0.34364932618368726 1101 46-47 0.40620930581058445 1101 48-49 0.39839808055833714 1101 50-51 0.39224260707359093 1101 52-53 0.2628800711571202 1101 54-55 0.3053300818328495 1101 56-57 0.1920247355155702 1101 58-59 0.0999157452842816 1101 60-61 0.08559575866179969 1101 62-63 0.20157811040306584 1101 64-65 0.19460131484811072 1101 66-67 -0.012017054887763834 1101 68-69 -7.990952930095947 1101 70-71 -5.666224938570952 1101 72-73 -1.1640484385746284 1101 74-75 -0.2487801159081613 1101 76-77 -0.19893332788864626 1101 78-79 -0.29503485176798705 1101 80-81 -0.2822397587065133 1101 82-83 -0.19154282520695531 1101 84-85 -0.11145010332407068 1101 86-87 -0.2140075314680594 1101 88-89 -0.10201614546437554 1101 90-91 -0.4050549308264131 1101 92-93 -0.6106824224402523 1101 94-95 -0.32211591947113405 1101 96-97 -0.8929328728347734 1101 98-99 -0.6706559547935242 1101 100-101 -0.3900609673054696 1102 1 0.17440993821082884 1102 2 0.15994185469614308 1102 3 0.2976007293696519 1102 4 0.26768725970111973 1102 5 0.303847143555096 1102 6 0.3304765012747737 1102 7 0.40412323177996967 1102 8 0.20600236751093348 1102 9 0.35381352588976256 1102 10-11 0.31613703127410275 1102 12-13 0.3314136651072914 1102 14-15 0.5267516809738382 1102 16-17 0.43171721717143186 1102 18-19 0.418157744314108 1102 20-21 0.3665411054906471 1102 22-23 0.48592786425454904 1102 24-25 0.3636347606858976 1102 26-27 0.4913531543224252 1102 28-29 0.22601239947219653 1102 30-31 0.3810401026049419 1102 32-33 0.2696407124254989 1102 34-35 0.3648186208136437 1102 36-37 0.2433442270975874 1102 38-39 0.6941124662594476 1102 40-41 0.7383149076146012 1102 42-43 0.5382984962405146 1102 44-45 0.6660137655147125 1102 46-47 0.5004450561356606 1102 48-49 0.4575279770115017 1102 50-51 0.4508528592892489 1102 52-53 0.6115843874064169 1102 54-55 0.44974923380341636 1102 56-57 0.3202596379310627 1102 58-59 0.2538401734696336 1102 60-61 0.332617775847698 1102 62-63 0.4834441931206861 1102 64-65 0.4345039250913345 1102 66-67 0.5311523667360305 1102 68-69 -4.024569324412855 1102 70-71 0.47832473305134116 1102 72-73 0.8793582404361651 1102 74-75 0.5259729266790707 1102 76-77 0.40630352501851874 1102 78-79 0.33725945525318224 1102 80-81 0.2341310009965092 1102 82-83 0.307872364359266 1102 84-85 0.27757392427643524 1102 86-87 0.25400504186263717 1102 88-89 0.05532726091502127 1102 90-91 -0.14098764635424388 1102 92-93 -0.18731955458830996 1102 94-95 -0.012230806726122978 1102 96-97 -0.3575626089940691 1102 98-99 -0.1587636857515129 1102 100-101 -0.09325647038850349 1103 1 0.2243610710793611 1103 2 0.25392746084084905 1103 3 0.1602253187877949 1103 4 0.1881299734195423 1103 5 0.240587245149392 1103 6 0.2068426586639447 1103 7 0.1141437871746902 1103 8 0.21587483409299324 1103 9 0.2884113824112262 1103 10-11 0.4116742686087349 1103 12-13 0.29025208577384376 1103 14-15 0.38394338451758614 1103 16-17 0.2945529474248687 1103 18-19 0.30971443272610344 1103 20-21 0.22138123705098423 1103 22-23 0.2640143115524154 1103 24-25 0.3448269010227136 1103 26-27 0.3059493710559451 1103 28-29 0.22670676984804317 1103 30-31 0.24363548783539102 1103 32-33 0.26185643596015495 1103 34-35 0.16677722414966922 1103 36-37 0.3014135799690649 1103 38-39 0.6146297322831131 1103 40-41 0.46691549372994956 1103 42-43 0.3974483314998736 1103 44-45 0.4037264209771223 1103 46-47 0.4056480814718313 1103 48-49 0.36900772599334175 1103 50-51 0.38075573450500855 1103 52-53 0.43881622222027517 1103 54-55 0.4171319653853729 1103 56-57 0.369768535957288 1103 58-59 0.35078283966322843 1103 60-61 0.28400586358651125 1103 62-63 0.12699855680678596 1103 64-65 0.13084748823441927 1103 66-67 0.3040107202583471 1103 68-69 -3.19115255054324 1103 70-71 0.5992652602649287 1103 72-73 0.7300172755588257 1103 74-75 0.3820526943806186 1103 76-77 0.1881473359437109 1103 78-79 0.14407665590300311 1103 80-81 0.22346185868541113 1103 82-83 0.09503260719299433 1103 84-85 0.006694743996263242 1103 86-87 -0.21217360650225459 1103 88-89 -0.1367258261308706 1103 90-91 -0.1653479295296556 1103 92-93 -0.22611940396000207 1103 94-95 -0.3580368677015642 1103 96-97 -0.6223809226680892 1103 98-99 -0.19308549921184692 1103 100-101 -0.43573996548763816 1104 1 0.21204509141375638 1104 2 0.23484159620626244 1104 3 0.2073015039870043 1104 4 0.2195369058799983 1104 5 0.1999579344687774 1104 6 0.2252010918334122 1104 7 0.13849975767458744 1104 8 0.07201359418904474 1104 9 0.24177295444922464 1104 10-11 0.3025951659637016 1104 12-13 0.26522947855139734 1104 14-15 0.21295672356837514 1104 16-17 0.3433627916464488 1104 18-19 0.35283738057457015 1104 20-21 0.32277458587805796 1104 22-23 0.26250821611186836 1104 24-25 0.09555547293754785 1104 26-27 0.1845333220159091 1104 28-29 0.3448414818739707 1104 30-31 0.24657609356507493 1104 32-33 0.22708061503813326 1104 34-35 0.40286952758789596 1104 36-37 0.3575637094715134 1104 38-39 0.2822389952613449 1104 40-41 0.2857575126698393 1104 42-43 0.5311000023639849 1104 44-45 0.5537010854480471 1104 46-47 0.5440566558289817 1104 48-49 0.5060049499632768 1104 50-51 0.647666498350155 1104 52-53 0.4935822844018034 1104 54-55 0.5608688085433542 1104 56-57 0.5268824156514249 1104 58-59 0.5058280312991279 1104 60-61 0.46314016048873086 1104 62-63 0.6852078121352321 1104 64-65 0.5675232282020559 1104 66-67 0.8640859267762444 1104 68-69 -3.4841202217765037 1104 70-71 1.1598157711337649 1104 72-73 1.352698697422401 1104 74-75 0.7094326462571487 1104 76-77 0.7609745248263984 1104 78-79 0.518050645929506 1104 80-81 0.4804331646196438 1104 82-83 0.44561623672158746 1104 84-85 0.5043291003773227 1104 86-87 0.655861009129346 1104 88-89 0.5106700183510888 1104 90-91 0.4342361396861669 1104 92-93 0.43329239418531174 1104 94-95 0.6036708662669561 1104 96-97 0.3254924544281046 1104 98-99 0.4918656176042404 1104 100-101 0.3670221510887792 1105 1 0.11614315214666249 1105 2 0.19441858393425449 1105 3 0.20624059754134194 1105 4 0.11069848987066422 1105 5 0.2100548664406574 1105 6 0.24522531931319946 1105 7 0.04431199487589765 1105 8 0.15823828209612145 1105 9 0.2593116684589347 1105 10-11 0.18565966225620656 1105 12-13 0.04917318367300538 1105 14-15 0.24422962979215157 1105 16-17 0.28919574824547567 1105 18-19 0.1457526493660879 1105 20-21 0.1968424123039867 1105 22-23 0.10634597647627686 1105 24-25 0.11363134081042148 1105 26-27 0.24868185977503288 1105 28-29 0.13180403750835978 1105 30-31 0.20048900457938146 1105 32-33 0.2948274814709606 1105 34-35 0.19923850996758574 1105 36-37 0.11166946321807103 1105 38-39 0.32795016984288594 1105 40-41 0.3616417554610152 1105 42-43 0.3272728001730911 1105 44-45 0.17077993196758712 1105 46-47 0.34814782932145505 1105 48-49 0.25146916778057005 1105 50-51 0.16787868303202202 1105 52-53 0.17125858376198977 1105 54-55 0.13500774657497772 1105 56-57 0.06218206743865906 1105 58-59 0.1594402107901196 1105 60-61 0.3416081023353179 1105 62-63 0.27921957710255185 1105 64-65 0.20010566007308128 1105 66-67 0.5882552551803926 1105 68-69 1.7922661788753125 1105 70-71 2.02773860147067 1105 72-73 1.1183131826122086 1105 74-75 0.8189102524627216 1105 76-77 0.43772630545795366 1105 78-79 0.4497827410976001 1105 80-81 0.3957172234231052 1105 82-83 0.45872175620017686 1105 84-85 0.44540019179627777 1105 86-87 0.31665602059592146 1105 88-89 0.4192079742554462 1105 90-91 0.3662670494855398 1105 92-93 0.36495324203853485 1105 94-95 0.6017093598340892 1105 96-97 0.22018001714058144 1105 98-99 0.4380153702997198 1105 100-101 0.43220818546153694 1106 1 0.3867020980909892 1106 2 0.3959760186718846 1106 3 0.3222548677231458 1106 4 0.19333340389934506 1106 5 0.36942727677751463 1106 6 0.41395556619170293 1106 7 0.23265046084718222 1106 8 0.3681941631633947 1106 9 0.39211174923487846 1106 10-11 0.43628540314943365 1106 12-13 0.5235305162445485 1106 14-15 0.5886138636371001 1106 16-17 0.5379679697650488 1106 18-19 0.5840519323212945 1106 20-21 0.44436315203711985 1106 22-23 0.4626854718482818 1106 24-25 0.5341788264921234 1106 26-27 0.5100385578543722 1106 28-29 0.5422306952577785 1106 30-31 0.6086990733919748 1106 32-33 0.5476151925513193 1106 34-35 0.6893538355665783 1106 36-37 0.8235268859413267 1106 38-39 0.8588350938542177 1106 40-41 0.5989772857404674 1106 42-43 0.5276289742290672 1106 44-45 0.6668219636021391 1106 46-47 0.6800121440338032 1106 48-49 0.7764025830390935 1106 50-51 0.842673809948927 1106 52-53 0.7924587327190196 1106 54-55 0.8469389617797205 1106 56-57 0.9230808651401325 1106 58-59 1.1205163039435 1106 60-61 1.1055358395306492 1106 62-63 0.9662465774109172 1106 64-65 0.8010957282559268 1106 66-67 1.2865250105886616 1106 68-69 2.820130888320275 1106 70-71 3.0775231080751375 1106 72-73 2.018619452193821 1106 74-75 1.6057365266671404 1106 76-77 1.2923998849878622 1106 78-79 1.1710366116847624 1106 80-81 1.1992363420084153 1106 82-83 1.1394932825400481 1106 84-85 1.1106010647815658 1106 86-87 1.1388475394386859 1106 88-89 1.235508759797959 1106 90-91 1.1036985363767755 1106 92-93 1.0164455124739966 1106 94-95 1.3287977618196436 1106 96-97 1.1703704439601665 1106 98-99 1.3071331904188064 1106 100-101 1.5017097968465833 1107 1 0.09804103848020773 1107 2 0.12720568286244216 1107 3 0.10385594820690969 1107 4 0.028528934625597913 1107 5 0.07376762916256041 1107 6 0.1095783791329552 1107 7 0.04104876645219235 1107 8 0.1188234221725466 1107 9 0.18623661877874298 1107 10-11 0.09904861758862893 1107 12-13 0.06444769961983354 1107 14-15 0.15952061052149702 1107 16-17 0.11232796730940464 1107 18-19 0.08603179603300504 1107 20-21 0.2095574549409136 1107 22-23 0.17724744618679011 1107 24-25 0.1383902575192053 1107 26-27 0.16426840621829797 1107 28-29 0.20777185800564268 1107 30-31 0.2394007739260786 1107 32-33 0.23129743209948117 1107 34-35 0.17856434572680513 1107 36-37 0.07277484468649931 1107 38-39 0.19877308882014688 1107 40-41 0.16626195637709174 1107 42-43 0.1106911634365062 1107 44-45 -0.04399515224291406 1107 46-47 0.1513885330079674 1107 48-49 0.18235138284264707 1107 50-51 0.08344979374167139 1107 52-53 0.20225982548100063 1107 54-55 0.24892803415985298 1107 56-57 0.1758588134488086 1107 58-59 0.1996034179468147 1107 60-61 0.20980866168056878 1107 62-63 0.1810694640215118 1107 64-65 0.1351555232095798 1107 66-67 0.7158024370727922 1107 68-69 2.09154104241199 1107 70-71 2.232597084378167 1107 72-73 1.2559421853266102 1107 74-75 0.6110315110124027 1107 76-77 0.23062506762645185 1107 78-79 0.23175243217711738 1107 80-81 0.3504733731337133 1107 82-83 0.3160081084962165 1107 84-85 0.2438198013522559 1107 86-87 0.3500943931436389 1107 88-89 0.179796315817363 1107 90-91 0.24878110743700077 1107 92-93 0.19366633634286146 1107 94-95 0.6877670048671014 1107 96-97 0.3602838824769563 1107 98-99 0.3269318275358799 1107 100-101 0.49406660205255193 1108 1 -0.21084418636042201 1108 2 -0.24241151415340667 1108 3 -0.19299275046206787 1108 4 -0.17604510168166598 1108 5 -0.07040486943576951 1108 6 -0.11475774435859165 1108 7 -0.15726009834985177 1108 8 -0.07778077732881883 1108 9 -0.16592339510875576 1108 10-11 -0.15714443591622995 1108 12-13 -0.28975714677361 1108 14-15 -0.17977718991139824 1108 16-17 -0.22367769427273032 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-0.3011474736854858 2316 78-79 -0.44840257629967084 2316 80-81 -0.5598234441415357 2316 82-83 -0.3711283090347912 2316 84-85 -0.5012361179786318 2316 86-87 -0.550256428364662 2316 88-89 -0.3806650777939957 2316 90-91 -0.24730362381601978 2316 92-93 -0.3244311856273505 2316 94-95 -0.0871637540457364 2316 96-97 -0.18190351022797202 2316 98-99 -0.407106251628619 2316 100-101 -0.37794412859924265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 66.0 5 205.0 6 556.0 7 1213.0 8 2592.0 9 4407.0 10 7528.0 11 12007.0 12 16525.0 13 20761.0 14 25218.0 15 32487.0 16 39651.0 17 46256.0 18 37966.0 19 26422.0 20 23883.0 21 24734.0 22 25299.0 23 27997.0 24 32223.0 25 37043.0 26 44000.0 27 53510.0 28 63942.0 29 76238.0 30 89996.0 31 105834.0 32 125510.0 33 151767.0 34 187403.0 35 240320.0 36 285776.0 37 318831.0 38 204846.0 39 20118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57480704322787 20.68053918884583 15.018235611814207 19.72641815611209 2 15.378244819356212 23.126640906796077 39.90158911486576 21.59352515898195 3 18.62591446712477 29.906214025296357 31.039585538262372 20.428285969316505 4 11.763557764189303 18.42073202064019 38.13731893006503 31.678391285105477 5 11.43800898077041 41.18229405807167 32.78316859747153 14.596528363686392 6 27.507806170759856 38.50063506073999 18.494170494642905 15.497388273857254 7 25.761450979547117 36.9339155860624 20.88327484837284 16.421358586017647 8 26.900954600531417 33.87460238791027 20.167490207844 19.056952803714307 9 28.63113698409883 16.144335467744312 20.75700866417256 34.4675188839843 10-11 24.34022085600765 28.57554588265326 26.59496526540348 20.489267995935613 12-13 26.578120104957986 26.811238063349407 27.79136533236973 18.81927649932288 14-15 23.000025692495637 26.89865470777687 28.42373224109838 21.677587358629115 16-17 19.537943797893874 30.638962052886935 28.725382778014446 21.09771137120475 18-19 19.48622467838797 27.60343715864204 33.79021082764645 19.120127335323534 20-21 21.275810598237328 26.255057692084556 33.617656711791525 18.851474997886587 22-23 20.57691255766308 27.060103035195404 33.400368645872874 18.962615761268644 24-25 19.864140372396687 27.363915044739194 33.83503529097312 18.936909291891002 26-27 19.664732818100823 27.511012576922276 33.894101071213974 18.930153533762926 28-29 19.25657489883576 27.682826009933052 33.93687931359569 19.123719777635504 30-31 19.460765929937903 27.69052419145969 33.639151397864616 19.209558480737794 32-33 18.968108696562062 28.01714224382465 33.29998760931465 19.71476145029864 34-35 19.617962097072635 27.768156518344554 33.49058187139229 19.12329951319052 36-37 19.75773693116992 27.50892728810174 33.12814622479987 19.605189555928472 38-39 19.476441419301054 27.64051258759677 33.23648173611154 19.646564256990633 40-41 19.345631121524846 27.66958787358633 33.37497399619074 19.60980700869808 42-43 19.191362117839194 27.849280090065744 32.809595286979814 20.149762505115252 44-45 19.26104933416956 27.888887782766393 32.5605270231257 20.289535859938347 46-47 19.610353304611944 28.14615971354375 31.80440393715582 20.439083044688484 48-49 19.50763718774964 28.32182204145558 31.20504395333655 20.96549681745823 50-51 19.399698793806483 28.587213544075357 31.05878829833432 20.954299363783832 52-53 19.664032849863318 28.876371785344624 30.564729358528623 20.894866006263435 54-55 18.99257913358679 28.57131010198975 30.77150971114722 21.66460105327624 56-57 19.417588214141 28.743907734366335 30.131809528546988 21.70669452294568 58-59 19.467465393974773 28.193515645576777 30.466315917953068 21.872703042495385 60-61 19.467414300781822 28.603532198205926 30.45429962141698 21.474753879595266 62-63 19.508680281445585 28.547150506420543 30.35647315314285 21.58769605899102 64-65 19.021063005015236 28.7175627816461 30.27901250121216 21.9823617121265 66-67 19.02816895270992 28.72377842579149 30.038465121905823 22.20958749959277 68-69 19.289898188673295 28.49013392679761 29.90743346878082 22.31253441574827 70-71 19.161597560006673 28.748209710637656 29.675582720208194 22.414610009147477 72-73 19.259461439710076 28.720558673016672 29.305405217477936 22.71457466979531 74-75 19.138353267326075 29.11575394762695 28.849096897692068 22.89679588735491 76-77 18.955509806396496 29.02263920141948 29.080506153441522 22.941344838742506 78-79 19.00846567687703 29.023434359508638 28.893287217192604 23.07481274642173 80-81 18.98736904919772 29.301153693144144 28.519282091234583 23.192195166423552 82-83 18.963224492417602 29.227132115425658 28.23647880057435 23.573164591582387 84-85 18.85001203822229 28.924843782827985 28.11202550031142 24.11311867863831 86-87 18.893650578886177 28.957507597512024 28.074959217827665 24.07388260577413 88-89 18.936953553851513 28.84564342159489 27.94219753065444 24.275205493899154 90-91 18.918699960197113 28.799160762019138 27.982797527621152 24.299341750162597 92-93 19.00468877332084 28.791044896729503 27.892016280272987 24.312250049676674 94-95 18.68464929296255 28.25491542236771 28.301631711008433 24.758803573661304 96-97 18.817109153100983 28.07206414875535 28.245516454759205 24.86531024338446 98-99 18.743464350948656 28.35247970244483 27.833047142882606 25.071008803723917 100-101 18.586103352577535 28.415959902765696 28.135533724599654 24.862403020057116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6508.0 1 4397.5 2 2931.0 3 3687.5 4 3218.5 5 3140.0 6 5298.5 7 9965.0 8 14158.5 9 14864.0 10 12174.5 11 9338.5 12 8517.0 13 8248.0 14 8193.5 15 8181.5 16 8315.0 17 8464.0 18 8638.5 19 9114.0 20 9687.5 21 10623.0 22 12006.5 23 13365.5 24 14675.5 25 16631.5 26 19590.0 27 23329.5 28 26457.5 29 30404.5 30 35857.5 31 40193.5 32 45657.0 33 51696.0 34 57060.0 35 61577.0 36 67040.5 37 72234.5 38 73607.0 39 73510.5 40 72980.0 41 73997.0 42 75876.0 43 77564.5 44 79419.5 45 80961.5 46 81508.5 47 82879.5 48 88356.5 49 87465.5 50 78747.0 51 70940.0 52 64098.5 53 60363.5 54 58114.0 55 54495.0 56 50750.5 57 46578.5 58 42491.5 59 38311.0 60 33751.5 61 28972.5 62 22766.5 63 15269.5 64 9637.0 65 7118.5 66 5243.5 67 3856.0 68 2716.0 69 1795.0 70 1381.0 71 1113.5 72 949.0 73 958.5 74 824.0 75 632.5 76 454.0 77 237.5 78 113.0 79 66.0 80 39.5 81 22.0 82 15.5 83 11.0 84 6.0 85 2.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023579080672778717 2 0.025609616618237692 3 8.287901818199901E-5 4 0.0 5 0.0 6 4.143950909099951E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3151607272799606E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4503828181849828E-4 26-27 2.4863705454599707E-4 28-29 4.143950909099951E-5 30-31 4.558346000009945E-4 32-33 0.002196293981822974 34-35 0.004019632381826952 36-37 0.1006772873365833 38-39 0.09328033496383988 40-41 0.1015889565365853 42-43 0.10245918622749628 44-45 0.09663693520021084 46-47 0.09021381129110592 48-49 0.09075252490928892 50-51 0.09046244834565192 52-53 0.07044716545469916 54-55 0.05979721161831228 56-57 0.032053460281888116 58-59 0.013157044136392343 60-61 0.008743736418200896 62-63 0.0017818988909129787 64-65 0.004558346000009946 66-67 0.14795976720941373 68-69 0.3995390269008717 70-71 0.3818650762735605 72-73 0.1754341617367464 74-75 0.028759019309153658 76-77 0.030064363845520144 78-79 0.0048898620727379416 80-81 0.0014918223272759822 82-83 4.143950909099951E-5 84-85 3.7295558181899555E-4 86-87 0.0012639050272754849 88-89 2.693568090914968E-4 90-91 6.008728818194929E-4 92-93 0.0021341347181864745 94-95 0.07423888053652561 96-97 0.016119969036398805 98-99 0.01769467038185679 100-101 0.00917885126365639 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.27743232708745 #Duplication Level Percentage of deduplicated Percentage of total 1 67.91644719620164 23.95917869860212 2 13.289953819427513 9.376708929899433 3 5.870330320590501 6.21270541866843 4 3.3618042415459564 4.743832865122122 5 2.152526635255323 3.796780635373646 6 1.4837849820667732 3.140647457568556 7 1.069984442339819 2.642241262897556 8 0.7703290172797499 2.174018382134253 9 0.5782229720719855 1.835839959051316 >10 3.1416524531029006 19.70963157448277 >50 0.22443761226405098 5.420793591081344 >100 0.12759449161701217 8.66153327975084 >500 0.008699585468410986 2.152896749013607 >1k 0.0036444209394694616 2.4590492754765587 >5k 2.3512393157867496E-4 0.6608282791466698 >10k+ 3.5268589736801246E-4 3.0533136417306466 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31571 1.3082867415119455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23754 0.9843540989476022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18297 0.7582186978380179 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9936 0.4117429623281711 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5998 0.24855417552781503 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4620 0.19145053200041773 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4288 0.17769261498220587 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4160 0.17238835781855794 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3782 0.15672422338216013 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3315 0.13737197263666334 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3055 0.1265977002730035 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 2800 0.1160306254547986 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 4.143950909099951E-5 0.0 0.0 0.0 2 0.0 4.143950909099951E-5 0.0 0.0 0.0 3 0.0 4.143950909099951E-5 0.0 0.0 0.0 4 0.0 4.143950909099951E-5 0.0 0.0 0.0 5 0.0 4.143950909099951E-5 0.0 0.0 0.0 6 0.0 4.143950909099951E-5 0.0 0.0 0.0 7 0.0 4.143950909099951E-5 0.0 0.0 0.0 8 0.0 4.143950909099951E-5 0.0 0.0 0.0 9 0.0 4.143950909099951E-5 0.0 0.0 0.0 10-11 0.0 4.143950909099951E-5 0.0 2.0719754545499754E-5 0.0 12-13 0.0 4.143950909099951E-5 0.0 1.0359877272749876E-4 0.0 14-15 0.0 4.143950909099951E-5 0.0 2.48637054545997E-4 0.0 16-17 0.0 4.143950909099951E-5 0.0 4.972741090919941E-4 0.0 18-19 0.0 8.287901818199901E-5 0.0 9.323889545474888E-4 0.0 20-21 0.0 8.287901818199901E-5 0.0 0.0014918223272759822 0.0 22-23 0.0 8.287901818199901E-5 0.0 0.0033980397454619594 0.0 24-25 0.0 8.287901818199901E-5 0.0 0.008453659854563898 0.0 26-27 0.0 8.287901818199901E-5 0.0 0.016886599954582297 0.0 28-29 0.0 8.287901818199901E-5 0.0 0.03721267916371755 0.0 30-31 0.0 1.243185272729985E-4 0.0 0.1000971342093093 0.0 32-33 0.0 1.243185272729985E-4 0.0 0.21911140431865989 0.0 34-35 0.0 1.6575803636399803E-4 0.0 0.3852838357735679 0.0 36-37 0.0 2.0719754545499752E-4 0.0 0.6432033403559487 0.0 38-39 0.0 2.0719754545499752E-4 0.0 1.040235276956815 0.0 40-41 0.0 2.0719754545499752E-4 0.0 1.51121601753057 0.0 42-43 0.0 2.0719754545499752E-4 0.0 1.9333810163951273 0.0 44-45 0.0 2.0719754545499752E-4 0.0 2.3999070097415998 0.0 46-47 0.0 2.0719754545499752E-4 0.0 2.942184425706419 0.0 48-49 0.0 2.0719754545499752E-4 0.0 3.5333397426440727 0.0 50-51 0.0 2.0719754545499752E-4 0.0 4.188229024563683 0.0 52-53 0.0 2.0719754545499752E-4 0.0 4.78655337657408 0.0 54-55 0.0 2.0719754545499752E-4 0.0 5.363018387538974 0.0 56-57 0.0 2.0719754545499752E-4 0.0 6.0038182363676444 0.0 58-59 0.0 2.6935680909149676E-4 0.0 6.671180810523646 0.0 60-61 0.0 2.900765636369965E-4 0.0 7.38447908050702 0.0 62-63 0.0 2.900765636369965E-4 0.0 8.052939801653935 0.0 64-65 0.0 2.900765636369965E-4 0.0 8.680934842173485 0.0 66-67 0.0 2.900765636369965E-4 0.0 9.323288672593069 0.0 68-69 0.0 2.900765636369965E-4 0.0 10.041849760230999 0.0 70-71 0.0 2.900765636369965E-4 0.0 10.890510186660126 0.0 72-73 0.0 2.900765636369965E-4 0.0 11.729411608698317 0.0 74-75 0.0 2.900765636369965E-4 0.0 12.528095987163699 0.0 76-77 0.0 2.900765636369965E-4 0.0 13.339398696147285 0.0 78-79 0.0 2.900765636369965E-4 0.0 14.25173092829473 0.0 80-81 0.0 2.900765636369965E-4 0.0 15.221643358324119 0.0 82-83 0.0 2.900765636369965E-4 0.0 16.175995252689837 0.0 84-85 0.0 2.900765636369965E-4 0.0 17.077470333455445 0.0 86-87 0.0 2.900765636369965E-4 0.0 17.96239033033919 0.0 88-89 0.0 2.900765636369965E-4 0.0 18.871759637586628 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16800 0.0 38.52893 1 GTATCAA 27255 0.0 35.467186 1 GTACATG 25500 0.0 28.73353 1 TACATGG 25915 0.0 27.720018 2 TATCAAC 34750 0.0 27.717884 2 ATCAACG 34995 0.0 27.502863 3 TCAACGC 35240 0.0 27.284735 4 CAACGCA 35770 0.0 26.867199 5 ACATGGG 25720 0.0 26.74231 3 AACGCAG 36390 0.0 26.409445 6 CATGGGA 18765 0.0 25.02583 4 TAAGGTG 830 0.0 24.574926 5 ATGGGAT 6905 0.0 23.494396 5 GTCGGGA 1275 0.0 23.444946 2 GTAAGGT 690 0.0 23.373926 4 ACGCAGA 41040 0.0 23.359352 7 CGCAGAG 41110 0.0 23.284962 8 CGTCACT 510 0.0 23.262249 1 ATGGGAG 6125 0.0 23.078735 5 GAGTACT 21390 0.0 22.575577 12-13 >>END_MODULE