FastQCFastQC Report
Fri 27 May 2016
SRR939859_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939859_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589871
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78620.21900508402669622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.14825045245358398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.10482270811402415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.034.8166961
GTATCAA133350.033.228471
TATCAAC165900.027.453232
ATCAACG166400.027.3726483
TCAACGC167500.027.1928884
CAACGCA168550.026.9386625
AACGCAG174250.026.0036
TACATGG149150.025.0652242
GTACATG146950.024.7618031
ACGCAGA195400.022.8727917
ACATGGG159450.022.614553
CGCAGAG199800.022.1306978
GTATAGG8650.021.3897131
AGAGTAC156850.020.59656710-11
GAGTACT86650.020.39341512-13
GCAGAGT209450.020.1597129
CATGGGA75500.019.9838474
CATGGGG89300.019.0208764
GTACTTT91250.019.0039714-15
AGTACTT94200.018.9603512-13
Produced by FastQC (version 0.11.5)