##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939859_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3589871 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95319831826826 31.0 30.0 33.0 16.0 34.0 2 29.775702525243943 31.0 30.0 33.0 25.0 34.0 3 30.058147493322185 31.0 30.0 34.0 25.0 34.0 4 33.826409918350826 35.0 35.0 37.0 28.0 37.0 5 33.77386624756154 35.0 35.0 37.0 28.0 37.0 6 33.8056506765842 35.0 35.0 37.0 28.0 37.0 7 33.71341198611315 35.0 33.0 37.0 28.0 37.0 8 33.57154978549369 35.0 33.0 37.0 28.0 37.0 9 34.85997324137831 37.0 34.0 39.0 28.0 39.0 10-11 34.671987795661735 37.0 34.0 39.0 27.0 39.0 12-13 34.54774670176171 37.0 34.0 39.0 27.0 39.0 14-15 35.227519874669596 38.0 33.5 40.0 27.0 41.0 16-17 35.03401584625186 38.0 33.0 40.0 26.5 41.0 18-19 34.840351505666916 38.0 33.0 40.0 25.0 41.0 20-21 35.007566149312886 38.0 34.0 40.0 25.5 41.0 22-23 34.889509818040814 38.0 33.5 40.0 25.0 41.0 24-25 34.67506255795821 38.0 33.0 40.0 24.5 41.0 26-27 34.55976301098285 38.0 33.0 40.0 24.0 41.0 28-29 34.62878108990546 38.0 33.0 40.0 24.0 41.0 30-31 34.42575109244872 38.0 33.0 40.0 24.0 41.0 32-33 34.09247351785064 38.0 33.0 40.0 23.0 41.0 34-35 33.909366381131804 38.0 33.0 40.0 22.5 41.0 36-37 33.705550004443054 38.0 32.0 40.0 21.0 41.0 38-39 33.466729445152765 38.0 32.0 40.0 19.5 41.0 40-41 33.22102061606114 37.0 31.0 40.0 18.0 41.0 42-43 32.82495875199972 37.0 31.0 40.0 16.0 41.0 44-45 32.46998123330894 37.0 30.5 40.0 14.5 41.0 46-47 32.09029600227975 37.0 30.0 39.5 11.0 41.0 48-49 31.716943310776347 36.0 30.0 39.0 9.0 40.5 50-51 31.065168079855795 35.5 29.0 38.5 8.0 40.0 52-53 31.19594339183776 35.5 29.5 39.0 8.0 40.0 54-55 31.274370583232656 36.0 29.5 39.0 7.0 40.0 56-57 30.94249528743512 35.5 29.0 39.0 4.0 40.0 58-59 30.507492051942812 35.0 28.0 39.0 2.0 40.0 60-61 30.05502481844055 35.0 28.0 38.0 2.0 40.0 62-63 29.58084872687626 34.0 27.0 38.0 2.0 40.0 64-65 29.004596822559918 34.0 26.0 37.5 2.0 40.0 66-67 28.385291282054425 34.0 26.0 37.0 2.0 39.0 68-69 27.805175450594184 33.0 25.5 36.0 2.0 39.0 70-71 27.207466507849446 32.5 24.0 36.0 2.0 38.5 72-73 26.542689138411937 32.0 22.5 35.0 2.0 38.0 74-75 26.17334842951181 32.0 21.5 35.0 2.0 37.0 76-77 25.603119722129293 31.0 20.0 35.0 2.0 37.0 78-79 24.942157113723585 31.0 18.0 34.5 2.0 36.0 80-81 24.21106231950953 30.5 12.5 34.0 2.0 36.0 82-83 23.453380358235712 30.0 7.0 34.0 2.0 35.0 84-85 22.653339493257555 29.5 2.0 34.0 2.0 35.0 86-87 21.69506689794703 29.0 2.0 34.0 2.0 35.0 88-89 20.661596614474448 28.0 2.0 33.5 2.0 35.0 90-91 19.195674301388546 25.5 2.0 32.5 2.0 34.5 92-93 17.607503723671407 21.5 2.0 32.0 2.0 34.0 94-95 17.081799318137058 19.5 2.0 31.0 2.0 34.0 96-97 16.763412250746615 19.0 2.0 31.5 2.0 34.0 98-99 15.858996604613369 4.5 2.0 31.0 2.0 34.0 100-101 14.253635019197068 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6494145382541596 1101 2 1.1914855847536998 1101 3 1.1360777760863137 1101 4 1.1436024562003624 1101 5 1.0052472821613492 1101 6 1.2443905788879377 1101 7 0.9295701545436685 1101 8 1.1880145087731364 1101 9 1.1076569368235383 1101 10-11 1.3027134980517516 1101 12-13 1.228138444016487 1101 14-15 1.590655413434689 1101 16-17 1.4208596167455312 1101 18-19 1.4543166761863588 1101 20-21 1.3368126716227593 1101 22-23 1.4692221790330322 1101 24-25 1.6664746921130842 1101 26-27 1.5540665056792093 1101 28-29 1.5173557115085998 1101 30-31 1.6740388859996145 1101 32-33 1.6973638438229628 1101 34-35 1.3822502572846886 1101 36-37 1.5490972646462495 1101 38-39 1.5484627917699072 1101 40-41 1.418230119200949 1101 42-43 1.528055067168907 1101 44-45 1.3846262308554174 1101 46-47 1.3080950967545633 1101 48-49 1.245936922337659 1101 50-51 1.233514773807947 1101 52-53 1.1073357333296272 1101 54-55 1.2392037475298672 1101 56-57 1.2437852664136209 1101 58-59 1.1267055747118064 1101 60-61 1.0193522931247863 1101 62-63 0.655465433694502 1101 64-65 0.8508536817459564 1101 66-67 0.9360152817847052 1101 68-69 0.6515329915151753 1101 70-71 0.8193457608301742 1101 72-73 0.7223069582938635 1101 74-75 0.6202202190917774 1101 76-77 0.661149941210283 1101 78-79 0.3277748416807533 1101 80-81 0.12236512091378415 1101 82-83 0.05917988268222629 1101 84-85 -0.07776957347782343 1101 86-87 -0.5176928709183315 1101 88-89 -4.480210529107108 1101 90-91 -7.954738764061624 1101 92-93 -10.503658512955205 1101 94-95 -8.57250486134075 1101 96-97 -5.396889650050991 1101 98-99 -3.8609034435613125 1101 100-101 -2.9337805967844037 1102 1 1.81165013340528 1102 2 1.1252442280092758 1102 3 1.0911996732571083 1102 4 0.9595569451532668 1102 5 0.9703382823263951 1102 6 0.9845995058950621 1102 7 1.0741384793996573 1102 8 1.158999254170034 1102 9 1.2988171845997485 1102 10-11 1.3263991057255637 1102 12-13 1.1682575192530607 1102 14-15 1.4324356211152036 1102 16-17 1.443282048179448 1102 18-19 1.4580722724454773 1102 20-21 1.5052839334832484 1102 22-23 1.499236421433146 1102 24-25 1.577824292252508 1102 26-27 1.586862748178575 1102 28-29 1.3774245920951955 1102 30-31 1.4992962360129454 1102 32-33 1.6636719113231564 1102 34-35 1.732981536434572 1102 36-37 1.7003691548343411 1102 38-39 1.5395488267157305 1102 40-41 1.6076233975729295 1102 42-43 1.728774227582889 1102 44-45 1.6149230706353421 1102 46-47 1.5330195277799064 1102 48-49 1.4219982639163469 1102 50-51 1.557089581610569 1102 52-53 1.5510469750153106 1102 54-55 1.5177369072767313 1102 56-57 1.5599663180198142 1102 58-59 1.3889794881517155 1102 60-61 1.2859815648349233 1102 62-63 1.3789701836618988 1102 64-65 1.3985917485421204 1102 66-67 1.3531645626820001 1102 68-69 1.3419524382775663 1102 70-71 1.2398078424596726 1102 72-73 1.29849687388975 1102 74-75 1.03514317278551 1102 76-77 1.0618945509696722 1102 78-79 1.0704614730992112 1102 80-81 0.99213441480698 1102 82-83 0.7616347346201273 1102 84-85 0.7358270134244762 1102 86-87 0.5460307895862471 1102 88-89 -0.03726674965425403 1102 90-91 -5.615365452749764 1102 92-93 -10.159223205652916 1102 94-95 -8.134611247683743 1102 96-97 -4.671127352707995 1102 98-99 -3.149673703490885 1102 100-101 -2.4419591276935293 1103 1 2.142197962108071 1103 2 1.3639093992359221 1103 3 1.0469173173448603 1103 4 0.9605819653008538 1103 5 1.0207124518186745 1103 6 0.9283756652634096 1103 7 0.8852588338486527 1103 8 0.9740202559415323 1103 9 1.146467994402805 1103 10-11 1.0430395279150204 1103 12-13 1.010868460971757 1103 14-15 1.1508547875064252 1103 16-17 1.062365992130161 1103 18-19 1.0839241347511077 1103 20-21 0.9871858286563722 1103 22-23 1.1064537145629103 1103 24-25 1.1042070481454402 1103 26-27 1.1333197437553864 1103 28-29 0.9886099387636875 1103 30-31 1.0567889104613428 1103 32-33 1.1358596979852749 1103 34-35 1.116999206890597 1103 36-37 1.1206695674420928 1103 38-39 1.0315165311533931 1103 40-41 0.7682368127098016 1103 42-43 0.9601246173505089 1103 44-45 0.9472048917544669 1103 46-47 1.0446349372494623 1103 48-49 1.0289490889461597 1103 50-51 1.0253133999591668 1103 52-53 0.970548354707276 1103 54-55 0.8047315927701 1103 56-57 0.9140357594209725 1103 58-59 0.8712150719489706 1103 60-61 0.9345470937044702 1103 62-63 0.8380824642203137 1103 64-65 0.7537038508361569 1103 66-67 0.7591605591612804 1103 68-69 0.6385336618396629 1103 70-71 0.586626380468978 1103 72-73 0.7772005947871641 1103 74-75 0.6013095231567078 1103 76-77 0.4784332139125951 1103 78-79 0.22145938220666395 1103 80-81 0.082092931201732 1103 82-83 -0.18465885194765264 1103 84-85 -0.17624204173720415 1103 86-87 -0.20711873337085862 1103 88-89 -0.8704226232796657 1103 90-91 -6.450999015474682 1103 92-93 -9.83501397527221 1103 94-95 -8.620312773701057 1103 96-97 -5.648931024211322 1103 98-99 -4.212162237006444 1103 100-101 -3.248375902371075 1104 1 1.94385658210971 1104 2 1.1028055956933898 1104 3 0.9972734909476699 1104 4 0.6926036265603344 1104 5 0.5957994389884149 1104 6 0.6549898300208312 1104 7 0.7687824037282951 1104 8 0.7457149467688353 1104 9 0.8219266980922626 1104 10-11 0.8771476928563331 1104 12-13 0.7730188626169863 1104 14-15 0.8498628867108948 1104 16-17 0.7855802610908 1104 18-19 0.8455488605609176 1104 20-21 0.6864822067501777 1104 22-23 0.7625225825069109 1104 24-25 0.8151197394843877 1104 26-27 0.7820499468640989 1104 28-29 0.7065569102091303 1104 30-31 0.8606726694579336 1104 32-33 0.6759708063860117 1104 34-35 0.6973316314167519 1104 36-37 0.7490591273555012 1104 38-39 0.6033764084738067 1104 40-41 0.5129030277874946 1104 42-43 0.7092828605067751 1104 44-45 0.6066591758764872 1104 46-47 0.6818637258589106 1104 48-49 0.49367711291915484 1104 50-51 0.6133623354413658 1104 52-53 0.46024052364158763 1104 54-55 0.5971958424975519 1104 56-57 0.7657542994000472 1104 58-59 0.4645233927572754 1104 60-61 0.5159028801416632 1104 62-63 0.5721172303166142 1104 64-65 0.5514951929623457 1104 66-67 0.5671441455908415 1104 68-69 0.44682685566828084 1104 70-71 0.3299534966355502 1104 72-73 0.2266944961468731 1104 74-75 0.25501921399339267 1104 76-77 0.13899998934840596 1104 78-79 -0.10224075145616851 1104 80-81 -0.17322556099147235 1104 82-83 -0.20964238646324773 1104 84-85 -0.1530105410920335 1104 86-87 0.019497839444106546 1104 88-89 -0.33358809936515144 1104 90-91 -3.0799201931836997 1104 92-93 -7.9249583647795845 1104 94-95 -5.676803208083365 1104 96-97 -3.042943373480991 1104 98-99 -2.102095712071165 1104 100-101 -1.4693243768197082 1105 1 1.7974157642085054 1105 2 1.0379164317736418 1105 3 0.8537218739458865 1105 4 0.7418044918846931 1105 5 0.634625014392789 1105 6 0.6407339036675594 1105 7 0.561141275460102 1105 8 0.7173093778398396 1105 9 0.7143288963633694 1105 10-11 0.6101549756227129 1105 12-13 0.5194838615528354 1105 14-15 0.6412886445745443 1105 16-17 0.5951153715203716 1105 18-19 0.6229624038548707 1105 20-21 0.5179623273563649 1105 22-23 0.5289667351353913 1105 24-25 0.6155330332311095 1105 26-27 0.3564084642729384 1105 28-29 0.3992379902935994 1105 30-31 0.6227155197200318 1105 32-33 0.7323646771493983 1105 34-35 0.6720381249293794 1105 36-37 0.6844149865552609 1105 38-39 0.5842093323424962 1105 40-41 0.5585666299932157 1105 42-43 0.5333653417012414 1105 44-45 0.5065806727507152 1105 46-47 0.6181856134587989 1105 48-49 0.45379650218804457 1105 50-51 0.3813909752804605 1105 52-53 0.18529727498252768 1105 54-55 0.32550549375956095 1105 56-57 0.6203266524258417 1105 58-59 0.423909843588536 1105 60-61 0.3354243382528104 1105 62-63 0.3617090848747715 1105 64-65 0.3297238298902734 1105 66-67 0.23842143662257698 1105 68-69 0.15027626583593445 1105 70-71 -0.10943396654705495 1105 72-73 -0.07589102557664873 1105 74-75 -0.07889805723192467 1105 76-77 -0.13746195024070573 1105 78-79 -0.30165341064743245 1105 80-81 -0.5270884812762766 1105 82-83 -0.7944697248276533 1105 84-85 -0.73542911926031 1105 86-87 -0.53562990369036 1105 88-89 -0.7612186163029584 1105 90-91 -3.705865387734155 1105 92-93 -8.201428106817513 1105 94-95 -6.104278430063033 1105 96-97 -3.6266427006738624 1105 98-99 -2.627218908803945 1105 100-101 -2.0501944639488023 1106 1 1.5897719385071412 1106 2 1.094750405851336 1106 3 0.7383619803164336 1106 4 0.5970248403232006 1106 5 0.6267993014521451 1106 6 0.7274772207792353 1106 7 0.7147130623485651 1106 8 -0.23284658654014123 1106 9 -0.5383958979626513 1106 10-11 -0.801793298832699 1106 12-13 -0.3335397278356851 1106 14-15 0.5560923612593314 1106 16-17 0.8580872061476796 1106 18-19 0.8363009897484233 1106 20-21 0.6802670239004271 1106 22-23 0.736879022984148 1106 24-25 0.8493460611974797 1106 26-27 0.8109038891156573 1106 28-29 0.5703421263195594 1106 30-31 0.7424992850367076 1106 32-33 0.884655067196789 1106 34-35 0.979986981787782 1106 36-37 0.8512027115507976 1106 38-39 0.8150713094476885 1106 40-41 0.682333674858917 1106 42-43 0.8196102467086348 1106 44-45 0.802511748539736 1106 46-47 0.807410393176518 1106 48-49 0.6675531475908905 1106 50-51 0.5533545898393761 1106 52-53 0.6660432814013646 1106 54-55 0.7599334075385578 1106 56-57 0.7799004563635812 1106 58-59 0.6578289196156959 1106 60-61 0.39612965225980545 1106 62-63 0.5720060070416935 1106 64-65 0.5735456173299909 1106 66-67 0.6406916024296514 1106 68-69 0.4500916360931946 1106 70-71 0.4344584920370096 1106 72-73 0.4515122343366009 1106 74-75 0.30588499696325044 1106 76-77 0.21858552483696414 1106 78-79 0.27537834340816403 1106 80-81 -0.0034704962969271946 1106 82-83 -0.10715181189759093 1106 84-85 -0.16100536195891024 1106 86-87 -0.1688515914076909 1106 88-89 -0.36595833756537743 1106 90-91 -3.758049681485769 1106 92-93 -8.584712258840957 1106 94-95 -5.9398103599696075 1106 96-97 -3.2970553537303644 1106 98-99 -2.247348518966037 1106 100-101 -1.7052488909282015 1107 1 1.7922427885830814 1107 2 0.9243555907408485 1107 3 0.7573740572297574 1107 4 0.6367264976466274 1107 5 0.6237217704926863 1107 6 0.5764482593137288 1107 7 0.4490872632064651 1107 8 0.4131543813251781 1107 9 0.6281480251106757 1107 10-11 0.5847629907908569 1107 12-13 0.5556007788565367 1107 14-15 0.5634745579856002 1107 16-17 0.6650365041256734 1107 18-19 0.7134260823099297 1107 20-21 0.5130934116002592 1107 22-23 0.4362490627439044 1107 24-25 0.4565421481530336 1107 26-27 0.527352672585117 1107 28-29 0.43527623670624394 1107 30-31 0.615691660359424 1107 32-33 0.8056201918018004 1107 34-35 0.7777024013109823 1107 36-37 0.35616104080735056 1107 38-39 0.4088469862307136 1107 40-41 0.46067980095158845 1107 42-43 0.5232797056936036 1107 44-45 0.33695706609518083 1107 46-47 0.3526545873744382 1107 48-49 0.3646662015978812 1107 50-51 0.37709514884413764 1107 52-53 0.4171298019450127 1107 54-55 0.23373940585744535 1107 56-57 0.25321237639488814 1107 58-59 0.15094900106981513 1107 60-61 -0.12622276912684782 1107 62-63 0.006715854529247167 1107 64-65 0.12725825083536435 1107 66-67 0.19811388829292298 1107 68-69 0.13579287146959018 1107 70-71 0.04735878578774688 1107 72-73 0.15113125897741853 1107 74-75 -0.0040878030194022585 1107 76-77 -0.09692908236871389 1107 78-79 -0.10501572676290394 1107 80-81 -0.19071261661687444 1107 82-83 -0.636022904857132 1107 84-85 -0.4513167994473761 1107 86-87 -0.18746566081158633 1107 88-89 -0.39058824443683804 1107 90-91 -3.6319013142929 1107 92-93 -4.799070660312157 1107 94-95 -3.0465540630078483 1107 96-97 -1.675359667318725 1107 98-99 -1.1117398381425563 1107 100-101 -0.7553909228004887 1108 1 1.685419134167013 1108 2 0.9835041353124687 1108 3 -0.34753743692172634 1108 4 -0.19311462542478353 1108 5 0.4467350822778826 1108 6 0.6962358736656782 1108 7 0.5060248935944713 1108 8 0.5635560262291079 1108 9 0.8000765120741633 1108 10-11 0.609192781423161 1108 12-13 0.6911431338147196 1108 14-15 0.6632089914363064 1108 16-17 0.6056507813862098 1108 18-19 0.6881470059326702 1108 20-21 0.521167641810429 1108 22-23 0.4027496911747974 1108 24-25 0.5653890492739322 1108 26-27 0.5456181627561492 1108 28-29 0.36099080437315934 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4.014463153209487 2311 94-95 3.416853016354647 2311 96-97 2.7063581735816413 2311 98-99 2.522420661890232 2311 100-101 2.211075956244054 2312 1 1.115670908773346 2312 2 0.42043107757442755 2312 3 -0.19156798075607284 2312 4 -0.554965690885382 2312 5 -0.1495256770973441 2312 6 -0.4475232158724296 2312 7 -0.007876811480933554 2312 8 0.10696631640747967 2312 9 0.005684974838537471 2312 10-11 -0.06486337487557847 2312 12-13 -0.03776689804904976 2312 14-15 0.12217051699551007 2312 16-17 -0.015049723994600583 2312 18-19 -0.43683206511030903 2312 20-21 0.1487202031066417 2312 22-23 0.4359348046888769 2312 24-25 0.4727963571381437 2312 26-27 0.3708445986418738 2312 28-29 0.40980758417066454 2312 30-31 0.3289431133474565 2312 32-33 0.374563637795724 2312 34-35 0.35984176372394217 2312 36-37 0.45739922854419035 2312 38-39 0.33620971902040253 2312 40-41 0.27779357549882633 2312 42-43 0.41569125023241327 2312 44-45 0.2411082218133771 2312 46-47 0.1988054693285619 2312 48-49 0.41037036411117 2312 50-51 0.21305089723996673 2312 52-53 0.18439919644859515 2312 54-55 0.39336235394175034 2312 56-57 0.30856015679870197 2312 58-59 0.3683087370297997 2312 60-61 0.28287373999622645 2312 62-63 0.15825313742151792 2312 64-65 0.30573596034171047 2312 66-67 0.3031480259577535 2312 68-69 0.3670439684680744 2312 70-71 0.4059454110446765 2312 72-73 0.19934398589926872 2312 74-75 0.49405188004540435 2312 76-77 0.676949253219977 2312 78-79 0.722179365063905 2312 80-81 0.820264346432058 2312 82-83 0.7962333779148913 2312 84-85 1.069852306517678 2312 86-87 1.4764261367919254 2312 88-89 1.724514182307729 2312 90-91 2.5592490180018217 2312 92-93 3.616078599201405 2312 94-95 3.1532613971821988 2312 96-97 2.3545119323812784 2312 98-99 2.1089233886668506 2312 100-101 1.7477613515553863 2313 1 -7.176181648576062 2313 2 -2.6575709781517496 2313 3 -1.3925982000962271 2313 4 -0.7356848147400967 2313 5 -0.37227082182389637 2313 6 -0.04115931100133707 2313 7 -0.05628517385920162 2313 8 0.01159413923306829 2313 9 0.09768957242279441 2313 10-11 0.027731112363760246 2313 12-13 -0.1986327419705347 2313 14-15 -0.14966847330520494 2313 16-17 -0.4087854637635573 2313 18-19 -0.48441553273965354 2313 20-21 -0.24503145169506269 2313 22-23 -1.0903915531062722 2313 24-25 -0.25069162690878954 2313 26-27 -0.10361601092868256 2313 28-29 -0.43439747337870926 2313 30-31 0.07665904778826871 2313 32-33 -0.2870490758765101 2313 34-35 -0.10938414355176462 2313 36-37 0.06270600320158337 2313 38-39 0.18938889149244176 2313 40-41 0.026010833470969885 2313 42-43 -0.05252487921922011 2313 44-45 -0.18468411429226705 2313 46-47 -1.0605275089135233 2313 48-49 -0.6988232955878146 2313 50-51 -0.6030077014972051 2313 52-53 -0.9191196172903346 2313 54-55 -0.6494295520446336 2313 56-57 -0.36493085523823154 2313 58-59 -0.20049980367950937 2313 60-61 -0.11709635214354464 2313 62-63 -0.28998125504691075 2313 64-65 -0.27768051353603695 2313 66-67 -0.12670791405952286 2313 68-69 -0.10046383868223785 2313 70-71 0.008014923360533999 2313 72-73 -0.03571029986403218 2313 74-75 0.1814798102664561 2313 76-77 0.39136783883655113 2313 78-79 0.29606397446249844 2313 80-81 0.4780376101278243 2313 82-83 0.7070121542059553 2313 84-85 0.8780886666628795 2313 86-87 1.2272687551000416 2313 88-89 1.7442216433370845 2313 90-91 2.5421491420770685 2313 92-93 3.3300982755557804 2313 94-95 2.9964708350859794 2313 96-97 2.2731844615672756 2313 98-99 1.8891213882092455 2313 100-101 1.699130178718752 2314 1 0.5722694041779874 2314 2 0.09592429695533156 2314 3 -1.2337626567495725 2314 4 -0.277858885332563 2314 5 -0.1381878124154312 2314 6 -3.3750863462918694 2314 7 -2.7207572165743947 2314 8 -2.5263999361443545 2314 9 -3.4135114667586812 2314 10-11 -2.614116347342808 2314 12-13 -1.2577802193036334 2314 14-15 -1.2566643171777656 2314 16-17 -2.1006744890193687 2314 18-19 -3.0679737574959844 2314 20-21 -2.7718899639618897 2314 22-23 -4.057279969199506 2314 24-25 -2.997843204084134 2314 26-27 -1.9180001489907141 2314 28-29 -1.9460293436816585 2314 30-31 -2.1084634637113666 2314 32-33 -2.2210617359134943 2314 34-35 -1.6185258213721667 2314 36-37 -1.8725560457135302 2314 38-39 -3.360140086905581 2314 40-41 -2.574861929067769 2314 42-43 -1.712798616218592 2314 44-45 -2.2061550645046637 2314 46-47 -1.6103609973517585 2314 48-49 -2.2533777583555867 2314 50-51 -3.070065899393395 2314 52-53 -2.0351406143761857 2314 54-55 -2.347534707326947 2314 56-57 -1.47044201673528 2314 58-59 -0.9117983872447155 2314 60-61 -1.3633702721054242 2314 62-63 -0.9644909432967417 2314 64-65 -1.4768649552816449 2314 66-67 -0.9005552395365832 2314 68-69 -0.7966584677635034 2314 70-71 -0.8882553060023568 2314 72-73 -1.942334369323671 2314 74-75 -2.054843385604176 2314 76-77 -1.1662312612272743 2314 78-79 -1.8700318961847167 2314 80-81 -0.9029689644106647 2314 82-83 -0.5850134730854393 2314 84-85 -1.6112033952291753 2314 86-87 -0.18787140715236816 2314 88-89 0.7398517390176416 2314 90-91 1.8225795913141631 2314 92-93 2.854357444806219 2314 94-95 2.6518296275675866 2314 96-97 1.7924068181614992 2314 98-99 1.820222749869279 2314 100-101 1.9381356492124109 2315 1 -2.8038310026693303 2315 2 -2.0648651403671536 2315 3 -2.858596002876844 2315 4 -4.161319697491923 2315 5 -3.8966985977075197 2315 6 -3.0362118548510146 2315 7 -5.829408786390932 2315 8 -3.2616722167922383 2315 9 -2.531570267710549 2315 10-11 -3.9941210299251324 2315 12-13 -2.9160019127096355 2315 14-15 -2.252800495883534 2315 16-17 -2.6534190963733266 2315 18-19 -2.2079111951255257 2315 20-21 -1.4662277239987347 2315 22-23 -0.9101734980242568 2315 24-25 -2.346137132079832 2315 26-27 -3.9333555646658525 2315 28-29 -3.111278671999763 2315 30-31 -3.2872391603414464 2315 32-33 -4.103618042046911 2315 34-35 -3.904806085439102 2315 36-37 -3.046613464919794 2315 38-39 -2.0663078922329774 2315 40-41 -1.69902136715789 2315 42-43 -3.147405509593888 2315 44-45 -6.547360100199761 2315 46-47 -3.6151347748907163 2315 48-49 -2.5166863482074326 2315 50-51 -3.6002809143623047 2315 52-53 -3.614157348850739 2315 54-55 -2.166421634478244 2315 56-57 -1.593736098258269 2315 58-59 -2.891799231644807 2315 60-61 -3.0979018603452353 2315 62-63 -5.092326976151583 2315 64-65 -4.42701740788009 2315 66-67 -3.989223313536737 2315 68-69 -2.3967003807135328 2315 70-71 -3.724642348299124 2315 72-73 -2.8707146564197004 2315 74-75 -1.9586643727846464 2315 76-77 -3.329961744070598 2315 78-79 -2.1425313204573797 2315 80-81 -1.7668507483977685 2315 82-83 -1.4936738707211212 2315 84-85 -2.8210326728832804 2315 86-87 -2.2409009611555923 2315 88-89 -1.521599093867902 2315 90-91 -0.6669351856874499 2315 92-93 -0.09379209077372863 2315 94-95 -0.06498683055784227 2315 96-97 -0.2844198495286818 2315 98-99 -0.5014146954366243 2315 100-101 -1.0240608657181163 2316 1 -7.407312242608242 2316 2 -6.025425506900358 2316 3 -3.323073486675913 2316 4 -4.02794602349184 2316 5 -2.096388200217117 2316 6 -2.8213201224862985 2316 7 -3.283558949432166 2316 8 -2.6137962106833506 2316 9 -7.34072319626134 2316 10-11 -6.85316585594569 2316 12-13 -5.834540980691635 2316 14-15 -4.5643943374652345 2316 16-17 -4.629447742099167 2316 18-19 -3.980315331006981 2316 20-21 -2.7021860349740905 2316 22-23 -3.1754542178822653 2316 24-25 -2.914708354269955 2316 26-27 -3.742214069639644 2316 28-29 -4.004827068416986 2316 30-31 -6.428011397927609 2316 32-33 -4.609142415679635 2316 34-35 -5.383510712508077 2316 36-37 -3.943276444240386 2316 38-39 -6.094848446160416 2316 40-41 -3.555300518517946 2316 42-43 -4.0977904520134985 2316 44-45 -2.6485827856333657 2316 46-47 -3.736115994191497 2316 48-49 -4.960789167376124 2316 50-51 -4.2741897350994975 2316 52-53 -5.876459697470047 2316 54-55 -4.912958968857243 2316 56-57 -4.135431231786978 2316 58-59 -2.732418334298874 2316 60-61 -2.7752332261767485 2316 62-63 -3.8399919780090137 2316 64-65 -2.5281035514389316 2316 66-67 -3.7223628273023692 2316 68-69 -2.6487292740969437 2316 70-71 -2.067791974748957 2316 72-73 -1.5902833194815749 2316 74-75 -1.7697046996926424 2316 76-77 -2.4762296682873775 2316 78-79 -4.552214731527677 2316 80-81 -5.633844680787252 2316 82-83 -3.08289172683207 2316 84-85 -1.8450329205408522 2316 86-87 -1.7492835095520114 2316 88-89 -0.8048091487653295 2316 90-91 -0.7133995023589321 2316 92-93 -0.8123418905838591 2316 94-95 -1.4601443820499789 2316 96-97 -3.0832529283250665 2316 98-99 -4.358389516916793 2316 100-101 -3.8309938743448555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29610.0 3 10073.0 4 9919.0 5 11054.0 6 12594.0 7 14542.0 8 17121.0 9 20536.0 10 23914.0 11 27978.0 12 32070.0 13 35226.0 14 38072.0 15 42564.0 16 47263.0 17 51291.0 18 49578.0 19 50016.0 20 54669.0 21 59688.0 22 66720.0 23 75159.0 24 84520.0 25 94404.0 26 107919.0 27 123055.0 28 139151.0 29 157157.0 30 178841.0 31 202158.0 32 224798.0 33 248742.0 34 282879.0 35 324100.0 36 336742.0 37 242168.0 38 62643.0 39 937.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.658817977215946 18.64582075277242 13.204854253301116 23.49050701671052 2 16.17073089787164 19.62162058145758 39.172962253253395 25.03468626741739 3 19.846310586708903 23.677529456486788 28.633766210585122 27.84239374621919 4 13.432225928540605 15.680097646721814 35.69273861287989 35.194937811857685 5 14.710905745182506 36.70716408902553 33.16932444364619 15.412605722145775 6 33.66542521558962 34.64462998026249 16.946448754439267 14.743496049708623 7 30.078629206648603 31.747588527613697 19.89427747906616 18.27950478667154 8 27.501559534025738 33.89143634478703 19.20467112524146 19.40233299594577 9 27.51621431212115 16.085462573503253 19.117723788199022 37.28059932617658 10-11 25.96402462713686 25.571921102208755 26.517123839456293 21.946930431198094 12-13 26.931126739169336 22.776564347504547 26.945312226481278 23.346996686844836 14-15 23.787623901866095 24.14469003958722 24.916780350900687 27.150905707645993 16-17 23.555065757319447 27.28856226580999 25.526767123348815 23.629604853521748 18-19 23.63060719143445 25.99087643427615 27.394301259024168 22.984215115265233 20-21 24.477383598297337 25.19196537752226 27.302506798083492 23.02814422609691 22-23 24.124770093182477 25.036568075794204 27.35008763638277 23.488574194640552 24-25 23.811471445204766 25.315124628110436 27.364567147973972 23.508836778710823 26-27 23.465188927960938 25.551303980713787 27.37502296576384 23.60848412556144 28-29 23.444901332436157 25.90148669692784 27.301909273724924 23.351702696911087 30-31 23.65091906016885 25.511555255282083 27.06789933890204 23.769626345647026 32-33 23.424988232430028 25.54150364696955 27.18339373926192 23.850114381338507 34-35 23.910236171675646 25.604987009622675 27.056721344333074 23.428055474368605 36-37 24.08547062950397 25.292708304543382 27.04398881177137 23.577832254181278 38-39 23.642284937641026 25.57825406039202 27.063999753241045 23.715461248725905 40-41 23.8110345551457 25.444675644576602 27.154692801276507 23.589596999001195 42-43 23.63926914829105 25.430488069542985 26.88155655252255 24.048686229643415 44-45 23.582755174126007 25.521669073085445 27.073844074669413 23.821731678119136 46-47 23.944852313590214 25.74414775354663 26.672255355629186 23.638744577233965 48-49 23.747315931324707 25.82162731708189 26.52291312875068 23.908143622842726 50-51 23.55209896795279 25.97587603089872 26.683917023465874 23.788107977682614 52-53 23.822762567457822 25.80795787258333 26.4539806677696 23.915298892189245 54-55 23.42406472696523 25.87481212435343 26.583238057364415 24.11788509131692 56-57 23.385101565135965 25.956989646586297 26.572412403882943 24.08549638439479 58-59 23.546762985623353 25.633405375252217 26.499862775081517 24.319968864042913 60-61 23.218246853681556 25.825245265677093 26.852696293135104 24.103811587506247 62-63 23.23429702464065 25.731755204974068 26.88042325797788 24.1535245124074 64-65 23.126545826804882 25.823366454239622 26.637160015785504 24.412927703169995 66-67 23.07401380519216 25.82358047399873 26.516631265159614 24.585774455649496 68-69 23.17783243449335 25.608705676005634 26.782575250518732 24.430886638982283 70-71 23.228872851207317 25.51828523657131 26.477591984446768 24.775249927774606 72-73 23.077185114800887 25.656165129998872 26.49943953427106 24.76721022092918 74-75 22.97682595466272 26.032651353939123 26.35308049527391 24.637442196124244 76-77 23.129410618060078 25.54754534386606 26.514779685355954 24.80826435271791 78-79 23.052038947143103 25.687741742344706 26.51860724015399 24.7416120703582 80-81 23.206192876203783 25.65296158722547 26.38056840276318 24.760277133807566 82-83 23.17317314521582 25.510221181262615 26.486018404913114 24.830587268608447 84-85 22.923199073196972 25.649829456459567 26.413872335898525 25.01309913444494 86-87 22.795852892996525 25.512311575574238 26.765270219687658 24.926565311741577 88-89 22.861430016903903 25.392366170914705 26.538821384506072 25.207382427675324 90-91 22.6019887906865 25.37362396464658 26.84761105755423 25.176776187112687 92-93 22.88455999049549 25.115051647149546 26.894191152735687 25.10619720961928 94-95 22.677928599639372 24.923728015290454 27.061663541250603 25.33667984381957 96-97 23.021590973211147 24.683135554350066 26.65486845802786 25.640405014410927 98-99 22.9381982340197 24.89783253099393 26.38356568972525 25.780403545261123 100-101 23.022171543875313 24.70950896734251 26.54387880795026 25.72444068083192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4239.0 1 2476.0 2 670.0 3 606.0 4 546.5 5 508.5 6 623.5 7 1024.5 8 1558.0 9 1809.0 10 1683.5 11 1624.0 12 1695.0 13 1834.0 14 2063.0 15 2260.0 16 2476.0 17 2794.0 18 3119.5 19 3487.0 20 4018.0 21 4651.0 22 5534.5 23 6693.0 24 8129.5 25 9947.0 26 12197.5 27 15119.5 28 18457.5 29 22198.0 30 26828.5 31 33075.0 32 40203.0 33 44703.0 34 47336.5 35 51639.0 36 58080.0 37 65590.5 38 72552.0 39 77975.0 40 82963.5 41 88829.5 42 94588.5 43 100968.5 44 108859.0 45 117223.5 46 124949.5 47 134076.5 48 143862.5 49 147682.0 50 147183.0 51 148545.5 52 150638.5 53 152529.0 54 152741.0 55 146645.5 56 137589.0 57 127811.5 58 116798.0 59 103801.5 60 88289.0 61 73566.5 62 60103.5 63 46456.0 64 34386.5 65 25549.5 66 18968.0 67 14311.5 68 10709.0 69 7948.5 70 6017.5 71 4303.5 72 3042.5 73 2299.5 74 1700.5 75 1249.0 76 877.0 77 582.0 78 386.5 79 250.0 80 168.5 81 114.0 82 90.5 83 77.5 84 56.5 85 45.5 86 34.5 87 25.0 88 16.5 89 9.0 90 8.5 91 8.5 92 6.5 93 6.0 94 4.5 95 2.5 96 2.5 97 2.0 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1293639799313123 2 0.11582588900826797 3 0.12056143521591722 4 0.13097963687274558 5 0.11958646982022474 6 0.13423880690977477 7 0.16605053496351263 8 0.15228959480716717 9 0.1628470772348087 10-11 0.1563705213919943 12-13 0.1454509089602384 14-15 0.1498939655491799 16-17 0.14415559779167553 18-19 0.16006146181854444 20-21 0.16592518226978073 22-23 0.19971469726906618 24-25 0.22269602445324638 26-27 0.235468071136818 28-29 0.23046789146462365 30-31 0.22148428174717144 32-33 0.2226542402220024 34-35 0.2118181962527344 36-37 0.2198407686515755 38-39 0.206901585043028 40-41 0.21162320317359593 42-43 0.20442238732255283 44-45 0.1975558453214614 46-47 0.21269566510885768 48-49 0.2512625105470364 50-51 0.25280852710306306 52-53 0.25390884519248746 54-55 0.25195891440110246 56-57 0.22578805756529968 58-59 0.22730621796716372 60-61 0.19701265031528986 62-63 0.1896446975392709 64-65 0.19045252600998755 66-67 0.19947791995868375 68-69 0.19791797532557578 70-71 0.20435274693714622 72-73 0.2360669784513148 74-75 0.23900580271547364 76-77 0.25333779403215323 78-79 0.249772206299335 80-81 0.26280888644745176 82-83 0.2622378352871176 84-85 0.2375433546219349 86-87 0.24032897003819914 88-89 0.24361599622939098 90-91 0.4398347461510455 92-93 0.586065627427838 94-95 0.3402350669425169 96-97 0.2771408777641314 98-99 0.3082422738867218 100-101 0.32726802717980674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3589871.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.5357480579567 #Duplication Level Percentage of deduplicated Percentage of total 1 71.84590160222736 33.43402775958 2 12.913478063350686 12.018767234160762 3 5.485452657822094 7.658089286047737 4 2.927587579892441 5.449499121419113 5 1.7598201452745181 4.094727345390588 6 1.0975141165604647 3.0644184250985225 7 0.7697890017084829 2.507589492890437 8 0.5415432880164899 2.0160897610890283 9 0.41662597101782134 1.7449201099518215 >10 2.0500457272001067 16.95093803374692 >50 0.13110847311307058 4.221555041191707 >100 0.056784540202089215 4.525648062317302 >500 0.002878805354503892 0.9226930698824847 >1k 0.0013475259050217026 1.015244560225916 >5k 1.2250235500197294E-4 0.37579269700764995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7862 0.21900508402669622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5322 0.14825045245358398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3763 0.10482270811402415 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.228492333011409E-4 2 0.0 0.0 0.0 0.0 2.228492333011409E-4 3 0.0 0.0 0.0 0.0 2.228492333011409E-4 4 0.0 0.0 0.0 0.0 2.228492333011409E-4 5 0.0 0.0 0.0 0.0 2.7856154162642614E-4 6 2.785615416264261E-5 0.0 0.0 0.0 3.62130004114354E-4 7 2.785615416264261E-5 0.0 0.0 0.0 3.62130004114354E-4 8 2.785615416264261E-5 0.0 0.0 0.0 3.62130004114354E-4 9 2.785615416264261E-5 0.0 0.0 2.785615416264261E-5 3.62130004114354E-4 10-11 2.785615416264261E-5 0.0 0.0 2.785615416264261E-5 3.899861582769966E-4 12-13 2.785615416264261E-5 0.0 0.0 1.1142461665057045E-4 9.192530873672062E-4 14-15 2.785615416264261E-5 0.0 0.0 1.6713692497585567E-4 0.0010167496269364554 16-17 2.785615416264261E-5 0.0 0.0 1.6713692497585567E-4 0.001128174243587026 18-19 2.785615416264261E-5 0.0 0.0 1.8106500205717697E-4 0.001364951553969488 20-21 2.785615416264261E-5 0.0 0.0 2.6463346454510487E-4 0.0015460165560266651 22-23 2.785615416264261E-5 0.0 0.0 3.6213000411435394E-4 0.001643513095595914 24-25 8.356846248792783E-5 0.0 0.0 7.939003936353144E-4 0.001643513095595914 26-27 8.356846248792783E-5 0.0 0.0 0.001991715022628947 0.001768865789327806 28-29 1.2535269373189177E-4 0.0 0.0 0.007911147782190502 0.0018106500205717698 30-31 1.3928077081321307E-4 0.0 0.0 0.02930467417910003 0.0018245780976530912 32-33 1.3928077081321307E-4 0.0 0.0 0.07937611128645013 0.0018802904059783765 34-35 1.6713692497585567E-4 0.0 0.0 0.15227566673008586 0.0021170677163608385 36-37 1.6713692497585567E-4 0.0 0.0 0.2692575861361035 0.00213099579344216 38-39 2.228492333011409E-4 0.0 0.0 0.4558102505633211 0.0022424204100927304 40-41 2.228492333011409E-4 0.0 0.0 0.6655253071767759 0.0022842046413366944 42-43 2.7856154162642614E-4 0.0 0.0 0.8734854260779844 0.002325988872580658 44-45 3.62130004114354E-4 0.0 0.0 1.120290951958998 0.0023677731038246217 46-47 3.899861582769966E-4 0.0 0.0 1.4057190355865155 0.00245134156631255 48-49 3.899861582769966E-4 0.0 0.0 1.7085015032573594 0.002590622337125763 50-51 3.899861582769966E-4 0.0 0.0 2.041145768190556 0.0028413277245895466 52-53 3.899861582769966E-4 0.0 0.0 2.360767281052717 0.003036320803728045 54-55 3.899861582769966E-4 0.0 0.0 2.6970467741041393 0.0031059611891346515 56-57 3.899861582769966E-4 0.0 0.0 3.083801618498269 0.0032173858057852217 58-59 3.899861582769966E-4 1.3928077081321306E-5 0.0 3.492423543910074 0.003287026191191828 60-61 3.899861582769966E-4 2.785615416264261E-5 0.0 3.9272859665430877 0.0033288104224357923 62-63 3.899861582769966E-4 2.785615416264261E-5 0.0 4.347426411701145 0.003454163116167684 64-65 3.899861582769966E-4 2.785615416264261E-5 0.0 4.775840134645507 0.0036073719640622184 66-67 3.899861582769966E-4 2.785615416264261E-5 0.0 5.250105087341579 0.0036491561953061824 68-69 4.178423124396392E-4 2.785615416264261E-5 0.0 5.770555543639312 0.003677012349468825 70-71 4.456984666022818E-4 2.785615416264261E-5 0.0 6.316299387916725 0.0037745088890380743 72-73 4.8748269784624576E-4 2.785615416264261E-5 0.0 6.862224297196194 0.003983430045257894 74-75 5.014107749275671E-4 2.785615416264261E-5 0.0 7.3941097047776925 0.004192351201477713 76-77 5.014107749275671E-4 2.785615416264261E-5 0.0 7.9395053471280725 0.004248063509802998 78-79 5.014107749275671E-4 2.785615416264261E-5 0.0 8.539136921633117 0.00437341620353489 80-81 5.292669290902096E-4 2.785615416264261E-5 0.0 9.160343087537129 0.004387344280616211 82-83 5.292669290902096E-4 2.785615416264261E-5 0.0 9.7454894618776 0.004456984666022818 84-85 5.43195006171531E-4 2.785615416264261E-5 0.0 10.304520691690593 0.0044709127431041395 86-87 5.571230832528523E-4 2.785615416264261E-5 0.0 10.861142364168517 0.004568409282673389 88-89 5.571230832528523E-4 2.785615416264261E-5 0.0 11.450829291637499 0.004665905822242637 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8380 0.0 34.816696 1 GTATCAA 13335 0.0 33.22847 1 TATCAAC 16590 0.0 27.45323 2 ATCAACG 16640 0.0 27.372648 3 TCAACGC 16750 0.0 27.192888 4 CAACGCA 16855 0.0 26.938662 5 AACGCAG 17425 0.0 26.003 6 TACATGG 14915 0.0 25.065224 2 GTACATG 14695 0.0 24.761803 1 ACGCAGA 19540 0.0 22.872791 7 ACATGGG 15945 0.0 22.61455 3 CGCAGAG 19980 0.0 22.130697 8 GTATAGG 865 0.0 21.389713 1 AGAGTAC 15685 0.0 20.596567 10-11 GAGTACT 8665 0.0 20.393415 12-13 GCAGAGT 20945 0.0 20.159712 9 CATGGGA 7550 0.0 19.983847 4 CATGGGG 8930 0.0 19.020876 4 GTACTTT 9125 0.0 19.00397 14-15 AGTACTT 9420 0.0 18.96035 12-13 >>END_MODULE