FastQCFastQC Report
Fri 27 May 2016
SRR939859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589871
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85730.2388108096363351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66680.18574483595650096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.13546447769293102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82050.036.211421
GTATCAA132850.034.5409971
ATCAACG163800.027.8063453
TCAACGC164350.027.6555254
TATCAAC167000.027.415842
CAACGCA167200.027.1559285
AACGCAG173300.026.209376
GTACATG149650.024.6441361
TACATGG149200.024.176392
ACGCAGA196950.022.8946537
CGCAGAG198900.022.5280638
ACATGGG153450.022.2767473
GTAAGGT16400.020.9871584
AGAGTAC168650.020.35266710-11
GCAGAGT217200.020.0876569
AGGTAAG14600.019.7004342
GAGTACT95400.019.55815712-13
TAAGGTG18400.019.2184355
GGTAAGG15950.018.9190773
GTACTTT99600.018.61527814-15
Produced by FastQC (version 0.11.5)