##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939859_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3589871 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.428347703858996 31.0 31.0 34.0 30.0 34.0 2 31.642429212637445 31.0 31.0 34.0 30.0 34.0 3 31.708099817514334 31.0 31.0 34.0 30.0 34.0 4 35.51454467305371 37.0 35.0 37.0 33.0 37.0 5 35.24467090878753 37.0 35.0 37.0 33.0 37.0 6 35.26056312329886 37.0 35.0 37.0 32.0 37.0 7 35.17706485831942 37.0 35.0 37.0 32.0 37.0 8 35.11334780553396 37.0 35.0 37.0 32.0 37.0 9 36.727044787960345 39.0 35.0 39.0 32.0 39.0 10-11 36.57753440165399 38.5 35.0 39.0 32.0 39.0 12-13 36.52261780437236 38.0 35.0 39.0 32.0 39.0 14-15 37.573618522782574 39.0 36.0 41.0 32.0 41.0 16-17 37.49722566632617 39.0 36.0 41.0 32.0 41.0 18-19 37.45492094284168 39.0 36.0 41.0 32.0 41.0 20-21 37.36218836275732 39.0 36.0 41.0 31.5 41.0 22-23 37.21492095398414 39.0 36.0 40.0 31.0 41.0 24-25 37.04736312251889 39.0 36.0 40.0 31.0 41.0 26-27 36.755986775012246 39.0 35.5 40.0 30.0 41.0 28-29 36.53256565486615 38.0 35.0 40.0 30.0 41.0 30-31 36.28229412700345 38.0 35.0 40.0 30.0 41.0 32-33 36.24579365108106 38.0 35.0 40.0 30.0 41.0 34-35 36.35758569040503 38.0 35.0 40.0 30.0 41.0 36-37 36.3448062618406 38.0 35.0 40.0 30.0 41.0 38-39 36.20977480806413 38.0 35.0 40.0 30.0 41.0 40-41 36.03990421939953 38.0 35.0 40.0 29.5 41.0 42-43 35.83384458661607 38.0 34.5 40.0 29.0 41.0 44-45 35.58818018809032 38.0 34.0 40.0 28.0 41.0 46-47 35.29801920458981 38.0 34.0 40.0 27.0 41.0 48-49 34.985485411592784 38.0 33.5 40.0 26.0 41.0 50-51 34.69839501196561 38.0 33.0 40.0 26.0 41.0 52-53 34.39225406706815 37.5 33.0 40.0 25.0 41.0 54-55 34.05019887901264 37.0 33.0 40.0 24.0 41.0 56-57 32.638501912742825 36.5 31.5 39.5 16.0 40.5 58-59 28.988263645128196 34.0 23.0 38.0 7.0 40.0 60-61 28.980576739387015 34.0 24.0 38.0 9.0 40.0 62-63 30.947064253840878 34.5 29.0 38.0 9.0 40.0 64-65 30.456124607263046 35.0 29.0 38.0 4.0 40.0 66-67 24.858818603788272 28.0 17.0 35.5 2.0 38.0 68-69 17.092857654216544 13.0 7.0 29.0 2.0 34.5 70-71 15.975132811179009 12.5 7.5 25.5 2.0 33.0 72-73 18.015979822116172 16.5 7.0 29.0 2.0 34.0 74-75 18.743560144640295 17.5 7.5 30.0 2.0 34.5 76-77 19.750275567005055 22.0 7.5 30.5 2.0 34.0 78-79 22.866369292935595 27.5 11.0 33.5 2.0 35.0 80-81 25.62346655353354 31.5 21.0 34.5 2.0 36.0 82-83 26.726237516612713 32.5 25.5 35.0 2.0 36.0 84-85 26.77129902439391 33.0 25.5 35.0 2.0 35.5 86-87 26.512678450005588 33.0 25.0 35.0 2.0 35.0 88-89 26.0957807397536 33.0 24.5 35.0 2.0 35.0 90-91 24.392489590851596 31.5 11.0 34.0 2.0 35.0 92-93 22.15979668907323 30.5 2.0 34.0 2.0 35.0 94-95 17.63019088986763 20.0 2.0 33.0 2.0 35.0 96-97 16.283101816193394 13.5 2.0 32.5 2.0 34.5 98-99 15.175138187416763 2.0 2.0 31.5 2.0 34.0 100-101 14.560521394779924 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36631902753967793 1101 2 0.4229958226034576 1101 3 0.46330487467243486 1101 4 0.4481936902753958 1101 5 0.41343688683807045 1101 6 0.40994820874409044 1101 7 0.46089631030966416 1101 8 0.4234151915041906 1101 9 0.4211589788734713 1101 10-11 0.5483161077519938 1101 12-13 0.48737372310471017 1101 14-15 0.5728821620041131 1101 16-17 0.5174838721899278 1101 18-19 0.6715777512354322 1101 20-21 0.6209718134634912 1101 22-23 0.5227752773060672 1101 24-25 0.5906321682221218 1101 26-27 0.672363154856825 1101 28-29 0.5754837384359774 1101 30-31 0.5096786441589813 1101 32-33 0.4807087661198679 1101 34-35 0.7752520054813345 1101 36-37 0.6657941453016036 1101 38-39 0.6365796628365175 1101 40-41 0.6752303807583715 1101 42-43 0.7146413509620757 1101 44-45 0.6168629979455034 1101 46-47 0.544166594948571 1101 48-49 0.5321134496993025 1101 50-51 0.5056505534315221 1101 52-53 0.6229505239819204 1101 54-55 0.6936784984623756 1101 56-57 1.366055347218591 1101 58-59 -8.132346287054244 1101 60-61 -9.609620725933262 1101 62-63 -2.3764160393048215 1101 64-65 0.3017601566198991 1101 66-67 -1.2337636977512716 1101 68-69 -3.887419231108998 1101 70-71 -4.439545111445279 1101 72-73 -6.906735158989939 1101 74-75 -7.871040061741478 1101 76-77 -8.801575686535838 1101 78-79 -11.80613333237266 1101 80-81 -8.084242901212939 1101 82-83 -4.186506113998426 1101 84-85 -3.230149892125816 1101 86-87 -3.018773967312761 1101 88-89 -3.1248820728357494 1101 90-91 -8.9304019963613 1101 92-93 -14.79687675681182 1101 94-95 -12.621326080768945 1101 96-97 -12.443559252269267 1101 98-99 -11.935043966992264 1101 100-101 -11.680660699015881 1102 1 0.41441655912629827 1102 2 0.43744158664359034 1102 3 0.44287510967204113 1102 4 0.27622213352566405 1102 5 0.4231756184334614 1102 6 0.39332580431763375 1102 7 0.3945657365856263 1102 8 0.4118295645558163 1102 9 0.5745316230777675 1102 10-11 0.5333925769755936 1102 12-13 0.535286934488937 1102 14-15 0.6598179338579442 1102 16-17 0.7077421974729532 1102 18-19 0.6494768125527699 1102 20-21 0.6874461961870182 1102 22-23 0.6735217337299702 1102 24-25 0.5510379496670623 1102 26-27 0.6634051583954914 1102 28-29 0.6691093987628136 1102 30-31 0.571253883619427 1102 32-33 0.5242089686758789 1102 34-35 0.5840818895915945 1102 36-37 0.6052563273434828 1102 38-39 0.48906960140030264 1102 40-41 0.4994825104819469 1102 42-43 0.7386685338638159 1102 44-45 0.5734634386058204 1102 46-47 0.4263137348691899 1102 48-49 0.43608966466027965 1102 50-51 0.5005135366949744 1102 52-53 0.3863679844257817 1102 54-55 0.36075137361515885 1102 56-57 1.0955251121266585 1102 58-59 -8.974017137570218 1102 60-61 -10.258585381928516 1102 62-63 -3.0069939183413688 1102 64-65 -0.09289311480400642 1102 66-67 -1.387947085388081 1102 68-69 -4.045673186722109 1102 70-71 -4.431172969302894 1102 72-73 -6.943735737226767 1102 74-75 -7.615886598149048 1102 76-77 -8.697047665132995 1102 78-79 -11.709068374794008 1102 80-81 -7.971147856852596 1102 82-83 -3.855840806938513 1102 84-85 -2.934484071462336 1102 86-87 -2.65867525155252 1102 88-89 -2.767845588515666 1102 90-91 -8.381606425586153 1102 92-93 -14.412469068685757 1102 94-95 -12.447636837474358 1102 96-97 -12.245601951769629 1102 98-99 -11.79237851450752 1102 100-101 -11.540004013570947 1103 1 0.32763151996384465 1103 2 0.30470073476271864 1103 3 0.26349472692357523 1103 4 0.1896903783272137 1103 5 0.2445001227285175 1103 6 0.1395883803121123 1103 7 0.2734945162545728 1103 8 0.32673989386211133 1103 9 0.4370110576825965 1103 10-11 0.40815052738096114 1103 12-13 0.34553082243492383 1103 14-15 0.42015819598084647 1103 16-17 0.4607511515438816 1103 18-19 0.44043862312459225 1103 20-21 0.41286001093320124 1103 22-23 0.32279474933181973 1103 24-25 0.4270119973453532 1103 26-27 0.27212729994483453 1103 28-29 0.3878973495766971 1103 30-31 0.4026805928219517 1103 32-33 0.39309385074285075 1103 34-35 0.4700664103018255 1103 36-37 0.1556408955908779 1103 38-39 0.26547965402543383 1103 40-41 0.33997340600044623 1103 42-43 0.3876971787203374 1103 44-45 0.283000025511555 1103 46-47 0.34408632152238994 1103 48-49 0.4946914777575131 1103 50-51 0.5859862900996333 1103 52-53 0.70207047604179 1103 54-55 0.4415328029240584 1103 56-57 1.1419165584078002 1103 58-59 -6.3395187103819275 1103 60-61 -8.63554174058158 1103 62-63 -2.232295203701778 1103 64-65 0.08438653371807447 1103 66-67 -1.4739364340917653 1103 68-69 -4.025486603344518 1103 70-71 -4.772068116426711 1103 72-73 -7.479566133530753 1103 74-75 -8.135411560732098 1103 76-77 -8.94180167886678 1103 78-79 -11.934032071997112 1103 80-81 -8.26643276417369 1103 82-83 -4.313487234409056 1103 84-85 -3.2914842905789605 1103 86-87 -3.0854414615405226 1103 88-89 -3.206718177768554 1103 90-91 -8.855256722879133 1103 92-93 -14.634231063464096 1103 94-95 -12.56534016099077 1103 96-97 -12.35905849797167 1103 98-99 -11.993013209953979 1103 100-101 -11.734211349892684 1104 1 0.10916784817020186 1104 2 0.12187800345002486 1104 3 0.12285841178952595 1104 4 0.0907589304091232 1104 5 -0.02598021907805048 1104 6 0.033188513912392636 1104 7 0.019329170892206093 1104 8 0.17154258638318254 1104 9 0.06914974428423193 1104 10-11 0.1125761305993791 1104 12-13 0.0751400791332344 1104 14-15 0.2235130892476036 1104 16-17 0.21156973474289487 1104 18-19 0.23807731942099508 1104 20-21 0.12839604767077617 1104 22-23 0.12058299538401229 1104 24-25 0.23814432229676896 1104 26-27 0.18273369887302238 1104 28-29 0.06304625822625098 1104 30-31 0.11785194957161593 1104 32-33 0.2288091908199945 1104 34-35 0.19998640024427772 1104 36-37 0.16825761601665334 1104 38-39 0.16956681570116672 1104 40-41 0.16864674142285452 1104 42-43 0.08157267526492973 1104 44-45 -0.04463137155586594 1104 46-47 0.030379876872373757 1104 48-49 0.08344046996979415 1104 50-51 0.020145931180216792 1104 52-53 0.10884205896122268 1104 54-55 -0.0012129706222197 1104 56-57 0.5376934196642864 1104 58-59 -6.5209712569496965 1104 60-61 -8.92627453438493 1104 62-63 -2.696812288088246 1104 64-65 -0.4460596324353965 1104 66-67 -1.716581507049657 1104 68-69 -4.260034848621128 1104 70-71 -4.971597638274046 1104 72-73 -7.814470177128477 1104 74-75 -8.609629489314742 1104 76-77 -9.137382987086262 1104 78-79 -12.053263413773035 1104 80-81 -8.550882247817352 1104 82-83 -4.95052506718228 1104 84-85 -4.072272150716476 1104 86-87 -3.8925256853860475 1104 88-89 -4.122306785217017 1104 90-91 -9.540705362764447 1104 92-93 -15.154527861247933 1104 94-95 -12.61769283321536 1104 96-97 -12.336240682849194 1104 98-99 -11.689416197446466 1104 100-101 -11.332309641172575 1105 1 0.1279710285794735 1105 2 0.09687410550395725 1105 3 0.059726765857046615 1105 4 0.06962262110378958 1105 5 0.07866694566285304 1105 6 0.09207392295282091 1105 7 0.09593433459217238 1105 8 0.033503432252558696 1105 9 0.13578901714321034 1105 10-11 0.12097533561507845 1105 12-13 0.13533443092086372 1105 14-15 0.2946817164270641 1105 16-17 0.17099673863795317 1105 18-19 0.215540339419789 1105 20-21 0.18499179860139492 1105 22-23 0.10608240652861411 1105 24-25 0.15582753658047466 1105 26-27 0.15435120945649317 1105 28-29 0.1859236807062956 1105 30-31 0.10915802180446121 1105 32-33 0.16544330750607372 1105 34-35 0.06414658596091982 1105 36-37 -0.037429883975150346 1105 38-39 0.13936520829179955 1105 40-41 0.07144521098890522 1105 42-43 0.07815294062948652 1105 44-45 0.007079816932808569 1105 46-47 -0.06655253464425215 1105 48-49 -0.02933363366324926 1105 50-51 -0.012441815880386287 1105 52-53 0.005475020360343308 1105 54-55 -0.07787268589709129 1105 56-57 0.4413911706313556 1105 58-59 -6.685402849864861 1105 60-61 -8.914458938640422 1105 62-63 -3.0502088973144623 1105 64-65 -0.7805314676277462 1105 66-67 -1.8413852820186385 1105 68-69 -4.052182923101338 1105 70-71 -4.7574429732816945 1105 72-73 -6.0199147193548725 1105 74-75 -6.725642559924541 1105 76-77 -8.694835499350203 1105 78-79 -11.940380436473964 1105 80-81 -8.080786050835808 1105 82-83 -4.202763583190606 1105 84-85 -3.200912075047146 1105 86-87 -2.9691241940561426 1105 88-89 -3.189319231499777 1105 90-91 -8.605511846953473 1105 92-93 -12.099335370901661 1105 94-95 -11.896568089892977 1105 96-97 -12.21282049842553 1105 98-99 -11.563902924169696 1105 100-101 -11.178097748168671 1106 1 0.06722230308560384 1106 2 0.07881458242944461 1106 3 0.05639545104300936 1106 4 0.09529224673415371 1106 5 0.050302017954692246 1106 6 0.02827736654489854 1106 7 0.10061547101945933 1106 8 0.19538596931356267 1106 9 0.08920353041637696 1106 10-11 0.07806325310955486 1106 12-13 0.06272027800439162 1106 14-15 0.20437507422150958 1106 16-17 0.1264884571044007 1106 18-19 0.18298957967742524 1106 20-21 0.15850579584290614 1106 22-23 0.1790754797090841 1106 24-25 0.08403696158637075 1106 26-27 0.23661359978391516 1106 28-29 0.25582992154165396 1106 30-31 0.17617815674114468 1106 32-33 0.13333500039420443 1106 34-35 0.06422372748401983 1106 36-37 0.15307015984977568 1106 38-39 0.25243097710785634 1106 40-41 0.25417363396397974 1106 42-43 0.14361026203876293 1106 44-45 0.09592466345183936 1106 46-47 -0.022388513092316487 1106 48-49 0.16272350439795957 1106 50-51 0.27144970418867587 1106 52-53 0.10412717638150326 1106 54-55 -0.1811065453684506 1106 56-57 0.6445571359153988 1106 58-59 -6.3366725187137725 1106 60-61 -8.619490426438901 1106 62-63 -2.470496415037374 1106 64-65 -0.24268357624080394 1106 66-67 -1.6256372207894252 1106 68-69 -3.951739285268351 1106 70-71 -4.3498852539200055 1106 72-73 -6.090536319477472 1106 74-75 -6.673269150576548 1106 76-77 -8.471270993188252 1106 78-79 -11.836375402946514 1106 80-81 -8.128317597894412 1106 82-83 -4.1677207629268445 1106 84-85 -3.192056993064206 1106 86-87 -3.0106984792412206 1106 88-89 -3.122282834482302 1106 90-91 -8.362480596471123 1106 92-93 -11.727302201493778 1106 94-95 -11.675470010803036 1106 96-97 -12.130620606857779 1106 98-99 -11.675284017213054 1106 100-101 -11.337444744314283 1107 1 0.02445187062047438 1107 2 0.0944341969066187 1107 3 0.042972712934798096 1107 4 2.0371846758848733E-4 1107 5 0.018471423848829716 1107 6 0.022567600500352114 1107 7 0.0069711662480642644 1107 8 -0.03694214981447885 1107 9 -0.05294883860215549 1107 10-11 0.05490011943850703 1107 12-13 0.021065204689008965 1107 14-15 0.07289410130839258 1107 16-17 0.07188752504065121 1107 18-19 0.12129094547380248 1107 20-21 0.2320961161334978 1107 22-23 0.04289496059749354 1107 24-25 0.14951084729042208 1107 26-27 0.23952946082269477 1107 28-29 0.17679383006087335 1107 30-31 0.19474552845034054 1107 32-33 0.15595315390064712 1107 34-35 0.018843609639048964 1107 36-37 0.06030967149195021 1107 38-39 0.12196574854487352 1107 40-41 0.06046829558358269 1107 42-43 0.16242279664509596 1107 44-45 0.15445369162834766 1107 46-47 0.16914923921422087 1107 48-49 0.06419327959303445 1107 50-51 0.13229533411833927 1107 52-53 0.06606045955187057 1107 54-55 -0.02037645053877668 1107 56-57 0.7784725736173428 1107 58-59 -6.642324148908056 1107 60-61 -8.841142980024507 1107 62-63 -2.850204995016071 1107 64-65 -0.5287900322526866 1107 66-67 -1.7458842015610934 1107 68-69 -3.9983626944891455 1107 70-71 -4.871727753158389 1107 72-73 -6.332578184516686 1107 74-75 -6.772214477582715 1107 76-77 -8.942680920284257 1107 78-79 -12.333197110592263 1107 80-81 -8.426689217314873 1107 82-83 -4.459826482894112 1107 84-85 -3.546237569436066 1107 86-87 -3.253156662567477 1107 88-89 -3.330821639988624 1107 90-91 -8.618714981929461 1107 92-93 -12.161214713640737 1107 94-95 -12.01391593098111 1107 96-97 -12.494901296971875 1107 98-99 -11.840118366869826 1107 100-101 -11.346739149096571 1108 1 0.027625286117896053 1108 2 0.04929207419243298 1108 3 0.005517082454193911 1108 4 0.020410771155411567 1108 5 -0.1305779202274735 1108 6 0.011910264148383476 1108 7 0.0033094029983757878 1108 8 0.005775469941404765 1108 9 0.018491739953063302 1108 10-11 0.10501602176490366 1108 12-13 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10.048326604079671 2316 100-101 9.109133643252825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 33.0 4 67.0 5 151.0 6 345.0 7 925.0 8 2137.0 9 3644.0 10 5808.0 11 8624.0 12 12458.0 13 16880.0 14 22120.0 15 30507.0 16 41575.0 17 54165.0 18 60646.0 19 64290.0 20 67700.0 21 67954.0 22 64862.0 23 61818.0 24 63487.0 25 68465.0 26 89774.0 27 131578.0 28 185726.0 29 244058.0 30 283582.0 31 298954.0 32 304720.0 33 313504.0 34 312886.0 35 296729.0 36 237100.0 37 134437.0 38 36757.0 39 1399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8619868875242 18.67166736182682 14.283521138023142 24.182824612625836 2 16.32053214578517 20.397621746483612 38.146075444334656 25.135770663396563 3 19.669726764082785 23.809814318804488 29.251327736851124 27.269131180261606 4 13.83849168953425 16.37975292148381 35.344055538485925 34.437699850496024 5 14.506659248609532 37.01107858587897 33.30499531447118 15.177266851040322 6 33.71375565131885 35.14848727112681 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25.71842609319168 26.546474011808442 23.922961647023783 54-55 23.344038905036633 25.783780884738533 26.713737749015987 24.158442461208846 56-57 23.28351517880332 25.7558715150237 26.820526039317233 24.14008726685575 58-59 21.59915188898538 25.502814989152277 28.642063718087584 24.255969403774756 60-61 23.058456802119437 25.73948199063197 27.19025972837542 24.01180147887317 62-63 23.03634935876276 25.79352046613556 26.915333953413743 24.25479622168794 64-65 22.286852360678576 25.842858374350225 27.30090034897485 24.56938891599635 66-67 21.897990499309067 25.595813705071652 28.155645168127187 24.350550627492098 68-69 22.251091908458243 24.971215828237426 28.876112151523948 23.901580111780383 70-71 21.53777445864475 25.163965266450145 28.86325580176573 24.435004473139372 72-73 21.364309679232708 25.36258351355629 28.772129805955416 24.500977001255585 74-75 21.993799182375074 25.60796172530463 27.959277241074826 24.43896185124547 76-77 20.744493457227758 25.728311725997333 28.506751955013733 25.02044286176118 78-79 21.807151390019612 25.88070478764328 27.234158924130135 25.077984898206978 80-81 22.75965050486592 25.890939043930356 26.274797596789185 25.07461285441454 82-83 22.973363336326948 25.81074101971551 26.122730554695107 25.09316508926244 84-85 22.733848681686062 25.829211897535554 26.041768370688683 25.395171050089704 86-87 22.806622659161814 25.81605238112284 26.066221855934085 25.311103103781264 88-89 22.671210935214926 25.76256243262291 25.915632042385933 25.650594589776233 90-91 22.218764823654524 25.797046702242042 26.29993765514669 25.684250818956745 92-93 22.045294308638166 25.739865038805217 26.402797061901794 25.812043590654827 94-95 20.359096369958987 25.598335185119065 28.038007026013297 26.00456141890865 96-97 20.123615858400267 25.374580575445055 28.347321559977836 26.154482006176842 98-99 20.547135961192424 25.527884573574127 27.489005790825278 26.43597367440817 100-101 20.5621510733633 25.414656100536188 27.449426878515137 26.573765947585375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 273.0 1 204.0 2 147.0 3 181.0 4 224.0 5 253.5 6 352.5 7 556.5 8 794.5 9 1012.5 10 1178.0 11 1332.0 12 1506.5 13 1741.5 14 2019.0 15 2270.0 16 2521.0 17 2826.0 18 3169.5 19 3617.5 20 4169.0 21 4820.5 22 5734.0 23 6906.0 24 8219.5 25 10066.0 26 12436.5 27 15198.5 28 18491.0 29 22440.5 30 27201.5 31 33064.0 32 39526.0 33 45067.5 34 50265.0 35 55865.0 36 62142.0 37 68601.5 38 74958.5 39 81470.0 40 88198.0 41 95531.5 42 102901.5 43 110406.0 44 118529.0 45 126837.0 46 135210.0 47 143168.0 48 149963.0 49 154345.5 50 156084.5 51 157409.0 52 157769.5 53 156477.0 54 152965.0 55 145392.0 56 134245.0 57 121120.0 58 106894.0 59 91588.5 60 75927.5 61 61154.0 62 47613.5 63 35779.5 64 26113.0 65 18801.5 66 13658.5 67 9965.0 68 7112.5 69 4842.0 70 3359.0 71 2443.0 72 1764.0 73 1276.5 74 893.0 75 597.5 76 357.0 77 192.5 78 116.0 79 67.5 80 41.5 81 28.5 82 17.5 83 12.0 84 7.5 85 6.0 86 3.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02144923870523481 2 0.004958395440950385 3 0.003231313882866543 4 0.0 5 0.06209136762853039 6 2.7856154162642615E-5 7 0.0 8 3.3427384995171134E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.089211562198196E-4 16-17 0.0 18-19 0.0012953111685628815 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.81065002057177E-4 28-29 0.0019360027143036616 30-31 0.009011465871614886 32-33 1.81065002057177E-4 34-35 8.774688561232422E-4 36-37 0.027828298008479968 38-39 0.027229390693983155 40-41 0.02923503379369342 42-43 0.030098574572735345 44-45 0.03061391342474423 46-47 0.03229921075158411 48-49 0.03238277921407204 50-51 0.02721546261690183 52-53 0.023050967569586762 54-55 0.017229031349594456 56-57 0.14579911088727143 58-59 0.7688716391201801 60-61 0.3063480554036621 62-63 3.899861582769966E-4 64-65 0.14964326016171614 66-67 1.4257058261982116 68-69 2.728078529841323 70-71 2.198240549590779 72-73 1.8834520794758363 74-75 1.5729256009477777 76-77 1.1238147554605722 78-79 0.43068399950861747 80-81 1.949930791384983E-4 82-83 1.949930791384983E-4 84-85 4.178423124396392E-5 86-87 0.002910968109996153 88-89 2.7856154162642615E-5 90-91 0.22786334105041658 92-93 0.17818189010134347 94-95 1.306300421380044 96-97 1.294600836631734 98-99 1.0237275935541974 100-101 0.4122710816071106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3589871.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.20258304722889 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78207010853697 25.788270664485534 2 14.853561988931851 11.645959948365265 3 6.6955266877673765 7.874458230664139 4 3.6512842129987026 5.725590903564696 5 2.228277463686304 4.367711616121544 6 1.408363162075856 3.312688429316198 7 0.9918977946726218 2.721946896901165 8 0.7305966393999878 2.2913020344083486 9 0.5405590175491226 1.9072178799658148 >10 2.825613844335973 19.79699466293647 >50 0.19196045438082396 5.206427893798417 >100 0.09353429903023491 6.2678819175933445 >500 0.004336988682578062 1.1804206726660051 >1k 0.0022751416015086732 1.4883262030547773 >5k 1.4219635009429208E-4 0.4248020461582429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8573 0.2388108096363351 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6668 0.18574483595650096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4863 0.13546447769293102 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.949930791384983E-4 2 0.0 0.0 0.0 0.0 1.949930791384983E-4 3 0.0 0.0 0.0 0.0 1.949930791384983E-4 4 0.0 0.0 0.0 5.571230832528522E-5 1.949930791384983E-4 5 0.0 0.0 0.0 1.1142461665057045E-4 2.5070538746378354E-4 6 2.785615416264261E-5 0.0 0.0 1.1142461665057045E-4 2.5070538746378354E-4 7 2.785615416264261E-5 0.0 0.0 1.1142461665057045E-4 2.7856154162642614E-4 8 5.571230832528522E-5 0.0 0.0 1.1142461665057045E-4 2.7856154162642614E-4 9 5.571230832528522E-5 0.0 0.0 1.3928077081321307E-4 2.7856154162642614E-4 10-11 5.571230832528522E-5 0.0 0.0 1.949930791384983E-4 2.7856154162642614E-4 12-13 8.356846248792783E-5 0.0 0.0 2.228492333011409E-4 8.356846248792785E-4 14-15 1.1142461665057045E-4 0.0 0.0 2.5070538746378354E-4 0.0010724619352617405 16-17 1.1142461665057045E-4 0.0 0.0 2.5070538746378354E-4 0.0012117427060749537 18-19 1.3928077081321304E-4 0.0 0.0 2.6463346454510487E-4 0.0013928077081321307 20-21 1.6713692497585567E-4 0.0 0.0 3.342738499517114E-4 0.0016574411726772353 22-23 1.6713692497585567E-4 0.0 0.0 4.596265436836031E-4 0.0017549377122464847 24-25 2.228492333011409E-4 0.0 0.0 8.913969332045636E-4 0.0017827938664091271 26-27 2.228492333011409E-4 0.0 0.0 0.0022145642559300875 0.0019220746372223402 28-29 2.646334645451048E-4 0.0 0.0 0.008287205863386177 0.0019499307913849828 30-31 3.0641769578906874E-4 0.0 0.0 0.030655697655988197 0.0019917150226289467 32-33 3.0641769578906874E-4 0.0 0.0 0.08312276402132555 0.002047427330954232 34-35 3.2034577287039007E-4 0.0 0.0 0.1595043387352916 0.0021170677163608385 36-37 3.3427384995171134E-4 0.0 0.0 0.2804836162636485 0.0021170677163608385 38-39 4.7355462076492444E-4 0.0 0.0 0.47390282269195744 0.002172780024686124 40-41 4.7355462076492444E-4 0.0 0.0 0.6916265236271721 0.0022006361788487663 42-43 4.8748269784624576E-4 0.0 0.0 0.9080827695479865 0.002256348487174052 44-45 5.571230832528523E-4 0.0 0.0 1.1636908401443953 0.002534910028800478 46-47 5.571230832528523E-4 0.0 0.0 1.4499824645509545 0.002799543493345583 48-49 5.571230832528523E-4 0.0 0.0 1.7281122357878598 0.002869183878752189 50-51 5.710511603341736E-4 0.0 0.0 2.0136796001861903 0.0029248961870774744 52-53 5.849792374154948E-4 0.0 0.0 2.2848314048053537 0.0030920331120533303 54-55 5.989073144968162E-4 0.0 0.0 2.57308688808038 0.003147745420378615 56-57 6.128353915781375E-4 0.0 0.0 2.824962234018994 0.003147745420378615 58-59 6.128353915781375E-4 0.0 0.0 2.988087872795429 0.0031616734974599364 60-61 6.128353915781375E-4 0.0 1.3928077081321306E-5 3.1096103453299575 0.003175601574541258 62-63 6.546196228221013E-4 0.0 2.785615416264261E-5 3.2111599553298715 0.0031895296516225792 64-65 6.685476999034227E-4 2.785615416264261E-5 2.785615416264261E-5 3.3212753327347975 0.0032034577287039004 66-67 6.964038540660653E-4 2.785615416264261E-5 2.785615416264261E-5 3.4128245833903224 0.0032034577287039004 68-69 6.964038540660653E-4 2.785615416264261E-5 2.785615416264261E-5 3.5171737368835814 0.0032034577287039004 70-71 7.521161623913505E-4 2.785615416264261E-5 5.571230832528522E-5 3.7071109240415603 0.003217385805785222 72-73 7.799723165539932E-4 2.785615416264261E-5 5.571230832528522E-5 3.953623960303866 0.003342738499517114 74-75 7.799723165539932E-4 2.785615416264261E-5 5.571230832528522E-5 4.233940439642538 0.0034680911932490053 76-77 7.799723165539932E-4 2.785615416264261E-5 5.571230832528522E-5 4.6348740665054535 0.0035098754244929694 78-79 7.799723165539932E-4 2.785615416264261E-5 5.571230832528522E-5 5.214783483863348 0.0035655877328182543 80-81 8.356846248792784E-4 2.785615416264261E-5 5.571230832528522E-5 5.868511709752244 0.0035795158098995755 82-83 8.356846248792784E-4 2.785615416264261E-5 5.571230832528522E-5 6.481347101330382 0.0038720054286073232 84-85 8.496127019605997E-4 2.785615416264261E-5 5.571230832528522E-5 6.978245179283601 0.0038720054286073232 86-87 8.63540779041921E-4 2.785615416264261E-5 5.571230832528522E-5 7.410182705729538 0.0039277177369326085 88-89 8.63540779041921E-4 2.785615416264261E-5 5.571230832528522E-5 7.849334976103599 0.004011286199420537 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8205 0.0 36.21142 1 GTATCAA 13285 0.0 34.540997 1 ATCAACG 16380 0.0 27.806345 3 TCAACGC 16435 0.0 27.655525 4 TATCAAC 16700 0.0 27.41584 2 CAACGCA 16720 0.0 27.155928 5 AACGCAG 17330 0.0 26.20937 6 GTACATG 14965 0.0 24.644136 1 TACATGG 14920 0.0 24.17639 2 ACGCAGA 19695 0.0 22.894653 7 CGCAGAG 19890 0.0 22.528063 8 ACATGGG 15345 0.0 22.276747 3 GTAAGGT 1640 0.0 20.987158 4 AGAGTAC 16865 0.0 20.352667 10-11 GCAGAGT 21720 0.0 20.087656 9 AGGTAAG 1460 0.0 19.700434 2 GAGTACT 9540 0.0 19.558157 12-13 TAAGGTG 1840 0.0 19.218435 5 GGTAAGG 1595 0.0 18.919077 3 GTACTTT 9960 0.0 18.615278 14-15 >>END_MODULE