FastQCFastQC Report
Fri 27 May 2016
SRR939858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767347
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.2212697688851067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56440.14981364870292013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.1036538444693308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133450.034.7917941
GGTATCA81850.033.7363171
ATCAACG163000.028.370523
TATCAAC164500.028.082852
TCAACGC165050.028.047024
CAACGCA166100.027.9259955
AACGCAG174050.026.8940536
TACATGG152850.024.837262
GTACATG154250.024.824331
ACGCAGA196600.023.4982287
CGCAGAG199450.022.950178
ACATGGG168400.021.9853823
GTATAGG7700.020.7714331
GCAGAGT207800.020.4432229
TAAGGTG17050.020.417795
AGAGTAC158700.020.24587210-11
GAGTACT88300.019.79244812-13
CATGGGG97800.019.6258494
GAGTACA114800.019.5868361
GTAAGGT16750.018.8177264
Produced by FastQC (version 0.11.5)