FastQCFastQC Report
Fri 27 May 2016
SRR939858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767347
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90650.24062025611126342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66890.177552001448234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.1277556858977949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143850.030.646081
GGTATCA95050.030.4541761
TATCAAC174850.024.9366592
TCAACGC174550.024.8373494
ATCAACG174700.024.816023
GTACATG155300.024.6587561
CAACGCA176650.024.5420825
TACATGG156400.024.3290982
AACGCAG184800.023.5110666
ACATGGG168300.022.0113033
GTATAGG7200.021.0904871
ACGCAGA207200.020.8319787
CGCAGAG210650.020.355698
AGAGTAC164750.019.66423410-11
GTACTTT90950.019.53141614-15
GAGTACT90800.019.51144412-13
GTAAGGT17100.019.416944
TAAGGTG18750.018.9731245
TAAGGTA10800.018.8852864
CATGGGA79400.018.6983244
Produced by FastQC (version 0.11.5)