##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939858_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3767347 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39375162415355 31.0 31.0 34.0 30.0 34.0 2 31.62588341344718 31.0 31.0 34.0 30.0 34.0 3 31.674542589254454 31.0 31.0 34.0 30.0 34.0 4 35.48758449911835 37.0 35.0 37.0 33.0 37.0 5 35.21674456852528 37.0 35.0 37.0 32.0 37.0 6 35.23376556499839 37.0 35.0 37.0 32.0 37.0 7 35.13583511155198 37.0 35.0 37.0 32.0 37.0 8 35.092091596553225 37.0 35.0 37.0 32.0 37.0 9 36.684898417905224 39.0 35.0 39.0 32.0 39.0 10-11 36.5404196905674 38.0 35.0 39.0 32.0 39.0 12-13 36.46199540419292 38.0 35.0 39.0 32.0 39.0 14-15 37.50392822853854 39.0 36.0 41.0 32.0 41.0 16-17 37.421875261291305 39.0 36.0 41.0 32.0 41.0 18-19 37.37530920300148 39.0 36.0 41.0 32.0 41.0 20-21 37.27620723548959 39.0 36.0 40.5 31.0 41.0 22-23 37.14323289041333 39.0 36.0 40.0 31.0 41.0 24-25 36.98710073693769 39.0 36.0 40.0 31.0 41.0 26-27 36.68652555764043 38.5 35.5 40.0 30.0 41.0 28-29 36.461302343532466 38.0 35.0 40.0 30.0 41.0 30-31 36.199005560146176 38.0 35.0 40.0 30.0 41.0 32-33 36.185333604788724 38.0 35.0 40.0 30.0 41.0 34-35 36.04590113414029 38.0 35.0 40.0 29.5 41.0 36-37 36.125631246604044 38.0 35.0 40.0 30.0 41.0 38-39 36.033656310395614 38.0 35.0 40.0 29.0 41.0 40-41 35.95420159066845 38.0 35.0 40.0 29.0 41.0 42-43 35.729561280126305 38.0 34.0 40.0 28.0 41.0 44-45 35.49217075039809 38.0 34.0 40.0 27.5 41.0 46-47 35.22033024831532 38.0 34.0 40.0 27.0 41.0 48-49 34.91268165634862 38.0 33.0 40.0 26.0 41.0 50-51 34.66833278166307 38.0 33.0 40.0 26.0 41.0 52-53 34.3997137773611 37.5 33.0 40.0 24.5 41.0 54-55 34.10524541540771 37.0 33.0 40.0 24.0 41.0 56-57 33.70644740184538 37.0 32.0 39.5 23.5 41.0 58-59 33.30679971874107 36.0 32.0 39.0 23.0 41.0 60-61 32.95465243844011 36.0 31.0 39.0 22.0 40.0 62-63 32.63074956992281 35.0 31.0 39.0 22.0 40.0 64-65 32.15427594007136 35.0 31.0 38.0 20.5 40.0 66-67 31.188316605823672 34.5 29.5 37.5 15.0 40.0 68-69 29.196093298546696 34.0 26.0 36.5 7.5 39.0 70-71 28.522293672443766 33.0 25.0 36.0 8.5 39.0 72-73 29.12893157970317 33.0 26.5 36.0 9.0 38.5 74-75 29.27013187264141 33.0 27.5 35.0 8.0 38.0 76-77 28.852731245621918 32.5 27.0 35.0 8.0 37.0 78-79 29.412902899573623 33.5 29.0 35.0 8.0 37.0 80-81 29.257580068945067 34.0 29.0 35.0 7.0 36.5 82-83 28.837383044354553 33.0 28.5 35.0 6.0 36.0 84-85 28.505146061671518 33.0 27.5 35.0 2.0 36.0 86-87 28.148841877321097 33.0 27.0 35.0 2.0 35.0 88-89 27.71724014273174 33.0 26.5 35.0 2.0 35.0 90-91 27.245719865995884 32.5 26.0 35.0 2.0 35.0 92-93 26.76482283155759 32.0 25.0 34.0 2.0 35.0 94-95 25.938581181929884 32.0 24.0 34.0 2.0 35.0 96-97 25.43258173457343 32.0 23.0 34.0 2.0 35.0 98-99 24.768053354256985 31.5 19.0 34.0 2.0 35.0 100-101 23.268720534636177 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2929816093400426 1101 2 0.3621612257263003 1101 3 0.32347615756068393 1101 4 0.29717006500192866 1101 5 0.27898390054642874 1101 6 0.26706729184792977 1101 7 0.2710449679838547 1101 8 0.2940127960708452 1101 9 0.39571689320369074 1101 10-11 0.36400529897847633 1101 12-13 0.345556543942358 1101 14-15 0.4129173168709741 1101 16-17 0.4658648318446126 1101 18-19 0.336633119135783 1101 20-21 0.4013952795853726 1101 22-23 0.3312784333442451 1101 24-25 0.2970515941365406 1101 26-27 0.32255635885073275 1101 28-29 0.33656078982217963 1101 30-31 0.13026391571807494 1101 32-33 0.13525045384810852 1101 34-35 0.20796316445196084 1101 36-37 0.48502952954331136 1101 38-39 0.46172513646216373 1101 40-41 0.46111714301167694 1101 42-43 0.31689070659518137 1101 44-45 0.15066808302478307 1101 46-47 0.10744366252988158 1101 48-49 0.1513221450758877 1101 50-51 0.18995897889330848 1101 52-53 -0.0878475933563223 1101 54-55 -0.3149564752738243 1101 56-57 -0.21659877084407952 1101 58-59 -0.24856846491604045 1101 60-61 -0.3456240870402496 1101 62-63 -0.28013222624694123 1101 64-65 -0.3535899557993467 1101 66-67 -0.5255610685743903 1101 68-69 -9.164633031785876 1101 70-71 -6.950544308022998 1101 72-73 -2.047397915200218 1101 74-75 -0.8848860059523211 1101 76-77 -0.6877769408221219 1101 78-79 -0.7807633350629324 1101 80-81 -0.8196135544526797 1101 82-83 -0.641292389137643 1101 84-85 -0.5420831155624946 1101 86-87 -0.5794014943819725 1101 88-89 -0.5367509414606815 1101 90-91 -0.8233268619728271 1101 92-93 -1.0566994939181278 1101 94-95 -0.8211979994917122 1101 96-97 -1.2784312248979468 1101 98-99 -1.000576187649422 1101 100-101 -0.8837310405701402 1102 1 0.1534368609128407 1102 2 0.07795139456458244 1102 3 0.20984988748242017 1102 4 0.17039334654204907 1102 5 0.19002594180714283 1102 6 0.21435011240081536 1102 7 0.28048588436942623 1102 8 0.11982273705403657 1102 9 0.2872971758472218 1102 10-11 0.29901061730939915 1102 12-13 0.23569428856036012 1102 14-15 0.35476984485909213 1102 16-17 0.3320263054824153 1102 18-19 0.24776474194061393 1102 20-21 0.2698193751251523 1102 22-23 0.25677716253985494 1102 24-25 0.31668043914915955 1102 26-27 0.2702507958979865 1102 28-29 0.15218133463083205 1102 30-31 0.07753291686550767 1102 32-33 0.05737810418535361 1102 34-35 0.041018540215965515 1102 36-37 -0.04778544359903947 1102 38-39 0.24204685344379584 1102 40-41 0.18333128692856349 1102 42-43 0.09444835904508153 1102 44-45 0.1890063239795765 1102 46-47 0.09785177219725938 1102 48-49 0.09659035925098891 1102 50-51 0.008507861829798458 1102 52-53 0.03438183751348589 1102 54-55 -0.1712917707385273 1102 56-57 -0.09889357483934447 1102 58-59 -0.13915766382772432 1102 60-61 -0.18815232892142575 1102 62-63 -0.1037454320952449 1102 64-65 -0.1779171846447234 1102 66-67 0.023744307571789136 1102 68-69 -4.834648101664065 1102 70-71 -0.04876453981600548 1102 72-73 0.24720890895671488 1102 74-75 0.13621906953006757 1102 76-77 -0.1074239161609043 1102 78-79 -0.18208746035113066 1102 80-81 -0.009445835177391615 1102 82-83 -0.011661718862850279 1102 84-85 -0.19374008367666207 1102 86-87 -0.3153262006355284 1102 88-89 -0.2798847606949053 1102 90-91 -0.4111391858862383 1102 92-93 -0.5413827008640304 1102 94-95 -0.26751933185360954 1102 96-97 -0.9390455830767586 1102 98-99 -0.6893993154639375 1102 100-101 -0.7227548707895721 1103 1 0.2227981323009054 1103 2 0.23137728205702146 1103 3 0.23940778740718116 1103 4 0.20348630256687272 1103 5 0.2866824454289656 1103 6 0.15207975304258525 1103 7 0.14243692669402463 1103 8 0.2915910245113409 1103 9 0.2590000139870341 1103 10-11 0.36551706632404546 1103 12-13 0.3616459188918739 1103 14-15 0.3841034299049255 1103 16-17 0.40891845714550357 1103 18-19 0.30361743888862236 1103 20-21 0.37082164023270536 1103 22-23 0.19954652262180872 1103 24-25 0.21068401903773548 1103 26-27 0.15725392166711316 1103 28-29 0.1405925966711763 1103 30-31 0.1311044031658355 1103 32-33 0.21771932508656988 1103 34-35 0.023203821561111226 1103 36-37 0.14136430389041266 1103 38-39 0.25806275280921653 1103 40-41 0.35116035502603893 1103 42-43 0.17047560987181498 1103 44-45 0.20610189309738303 1103 46-47 0.12212727783593635 1103 48-49 0.03923870214197933 1103 50-51 0.05391190767115717 1103 52-53 0.16119568483235724 1103 54-55 0.08612512219999502 1103 56-57 0.2490539363939277 1103 58-59 0.08086296521157976 1103 60-61 0.002974835808657872 1103 62-63 -0.07830769784936109 1103 64-65 0.011356538369255986 1103 66-67 0.11274006922525714 1103 68-69 -4.227357730856777 1103 70-71 0.3659979715712893 1103 72-73 0.5183195619158276 1103 74-75 0.34957997127893137 1103 76-77 0.09427413679814833 1103 78-79 -0.05155340184993307 1103 80-81 -0.0098042205128408 1103 82-83 -0.2592681982842855 1103 84-85 -0.23847969390249446 1103 86-87 -0.1995502007721619 1103 88-89 -0.41679137464427285 1103 90-91 -0.45190813302142985 1103 92-93 -0.3963539364074613 1103 94-95 -0.4171700502559368 1103 96-97 -0.7697058434772082 1103 98-99 -0.38526777392512557 1103 100-101 -0.381622079126096 1104 1 0.2206352571704535 1104 2 0.28289086569824207 1104 3 0.287247685348472 1104 4 0.20401983190421902 1104 5 0.22384560632475825 1104 6 0.26759069725115836 1104 7 0.16377332736289674 1104 8 0.2065368662389062 1104 9 0.2734519468663734 1104 10-11 0.3292675510074474 1104 12-13 0.2587223944107322 1104 14-15 0.3296572473734898 1104 16-17 0.41889888735465775 1104 18-19 0.27395783555991926 1104 20-21 0.2808979015181734 1104 22-23 0.2797366888864943 1104 24-25 0.31418079605170846 1104 26-27 0.28484549012737403 1104 28-29 0.254348681872024 1104 30-31 0.21443058733090226 1104 32-33 0.1935606744499765 1104 34-35 0.22930162508287566 1104 36-37 0.30129115762846226 1104 38-39 0.3490156092604053 1104 40-41 0.3646986544092812 1104 42-43 0.33538532312652336 1104 44-45 0.4191280864818623 1104 46-47 0.45768345875910654 1104 48-49 0.458971022782535 1104 50-51 0.41209291035347917 1104 52-53 0.41446800011527074 1104 54-55 0.35545013962080674 1104 56-57 0.32182740446511815 1104 58-59 0.3376031909795003 1104 60-61 0.2978056186208278 1104 62-63 0.4040414588287078 1104 64-65 0.4628658143848625 1104 66-67 0.6348503019083296 1104 68-69 -3.88915314558567 1104 70-71 0.9809433964318615 1104 72-73 1.1367848243891814 1104 74-75 0.8267090771620609 1104 76-77 0.7003100869244214 1104 78-79 0.4411575579216809 1104 80-81 0.34585071922208854 1104 82-83 0.4102330632519049 1104 84-85 0.4377309210778222 1104 86-87 0.3884432927194581 1104 88-89 0.28012224483542525 1104 90-91 0.2713092198586544 1104 92-93 0.08691072664740673 1104 94-95 0.5004332883532818 1104 96-97 0.10297765986334895 1104 98-99 0.22118020995505105 1104 100-101 0.2956196775103379 1105 1 0.19072630015836722 1105 2 0.15833945194439636 1105 3 0.1648510080137804 1105 4 0.13987253155760726 1105 5 0.16672374664162248 1105 6 0.2545437721915036 1105 7 0.16127610337566267 1105 8 0.16121316737804392 1105 9 0.21861441349301458 1105 10-11 0.1912437517753105 1105 12-13 0.10957834538189815 1105 14-15 0.20967323784035585 1105 16-17 0.22096710668847663 1105 18-19 0.13851825240090676 1105 20-21 0.24097151807606565 1105 22-23 0.24042893274884847 1105 24-25 0.23145823424470535 1105 26-27 0.32033722590966107 1105 28-29 0.2907265068007021 1105 30-31 0.3383975981780907 1105 32-33 0.18692643935857944 1105 34-35 0.30344071400991623 1105 36-37 0.09910699553489621 1105 38-39 0.3846359910219874 1105 40-41 0.27373784767811316 1105 42-43 0.2381082880750185 1105 44-45 0.2533951917079946 1105 46-47 0.363089415533814 1105 48-49 0.38503877223119787 1105 50-51 0.3743978419430505 1105 52-53 0.18711118446042718 1105 54-55 0.32364727020344475 1105 56-57 0.17998697590258672 1105 58-59 0.1778437020216117 1105 60-61 0.15212012159305743 1105 62-63 0.1674498105805 1105 64-65 0.17698768393339748 1105 66-67 0.7079384825917181 1105 68-69 1.8601515875998267 1105 70-71 2.2391298480280284 1105 72-73 1.3432451483858294 1105 74-75 0.8376068683730153 1105 76-77 0.5312877525208748 1105 78-79 0.5258607806298237 1105 80-81 0.6105210499698366 1105 82-83 0.5678443927211703 1105 84-85 0.17556061560253866 1105 86-87 0.07147726000799892 1105 88-89 0.21453493163700088 1105 90-91 0.267069291971044 1105 92-93 0.2964270092395971 1105 94-95 0.7493163367654567 1105 96-97 0.31912330574654746 1105 98-99 0.4271669645041243 1105 100-101 0.4305899844958745 1106 1 0.36951464044401305 1106 2 0.36538517623001354 1106 3 0.33463410449844844 1106 4 0.2604796124277726 1106 5 0.3297352485138063 1106 6 0.3391290579694015 1106 7 0.2519034880978239 1106 8 0.31664445974278976 1106 9 0.4433078931053629 1106 10-11 0.4030012719792424 1106 12-13 0.3875694877180038 1106 14-15 0.5738903308865488 1106 16-17 0.47803063280011315 1106 18-19 0.5164423756959877 1106 20-21 0.4993109271237586 1106 22-23 0.4867558841395052 1106 24-25 0.6072926670824828 1106 26-27 0.6233241680260235 1106 28-29 0.47111742137182233 1106 30-31 0.4662774304207318 1106 32-33 0.6135208779601768 1106 34-35 0.5567737139219915 1106 36-37 0.7953991324160796 1106 38-39 0.765213614669868 1106 40-41 0.6809645759839853 1106 42-43 0.6317768300313347 1106 44-45 0.7986632777790561 1106 46-47 0.746070986014935 1106 48-49 0.5955778358462638 1106 50-51 0.6836205474666883 1106 52-53 0.724904450840306 1106 54-55 0.6526786834743916 1106 56-57 0.6544229934889145 1106 58-59 0.7152230272657931 1106 60-61 0.7484561839931132 1106 62-63 0.7588646980723368 1106 64-65 0.7243817746836925 1106 66-67 1.2321759539630897 1106 68-69 2.7143981549493184 1106 70-71 2.9945176931420967 1106 72-73 1.8998706013143654 1106 74-75 1.357283266636248 1106 76-77 1.013930852589823 1106 78-79 0.9605583467024061 1106 80-81 0.9663631883917212 1106 82-83 0.938150181700081 1106 84-85 0.9768273977778108 1106 86-87 0.9978061914290492 1106 88-89 1.1279334378960613 1106 90-91 1.0682041404444043 1106 92-93 1.0652132991211012 1106 94-95 1.3716427918503662 1106 96-97 1.2765630376321262 1106 98-99 1.3043924062685122 1106 100-101 1.4248689060065303 1107 1 0.11946042879122842 1107 2 0.10195661243358245 1107 3 0.06135010347777836 1107 4 0.042641702507438595 1107 5 0.08009962564342032 1107 6 0.07972324006083653 1107 7 0.11288481096843839 1107 8 0.06314265969214716 1107 9 0.1945197272102277 1107 10-11 0.16990536039448045 1107 12-13 0.12563755713493663 1107 14-15 0.1784283689358901 1107 16-17 0.0592738135126325 1107 18-19 0.10994245971443917 1107 20-21 0.09579312405894314 1107 22-23 0.22879058225454685 1107 24-25 0.18518601868846218 1107 26-27 0.21779044669971626 1107 28-29 0.14682188090873893 1107 30-31 0.12640113578317624 1107 32-33 0.2655370654443132 1107 34-35 0.2146728010194323 1107 36-37 0.0117452691164317 1107 38-39 0.24571084583723746 1107 40-41 0.29608278465894955 1107 42-43 0.2049236463755406 1107 44-45 0.1633612718465116 1107 46-47 0.1829040437038998 1107 48-49 0.2166317397460844 1107 50-51 0.13697013532176783 1107 52-53 0.190678943930763 1107 54-55 0.22086106902627023 1107 56-57 0.21194769326893947 1107 58-59 0.2927531730355284 1107 60-61 0.49673516943068563 1107 62-63 0.5193490499076105 1107 64-65 0.22115949418039804 1107 66-67 0.7814235955201916 1107 68-69 2.1914511542610207 1107 70-71 2.4979382194185433 1107 72-73 1.3465465441583255 1107 74-75 0.7973584993337788 1107 76-77 0.4916079589651474 1107 78-79 0.5727371334816276 1107 80-81 0.594044479955592 1107 82-83 0.6820116779025369 1107 84-85 0.7286894220762399 1107 86-87 0.6787454582245758 1107 88-89 0.7761706134049682 1107 90-91 0.7577235332515144 1107 92-93 0.7179474020303083 1107 94-95 1.1405960842371492 1107 96-97 0.7421468371693543 1107 98-99 0.9741653662583225 1107 100-101 1.0131297773047407 1108 1 -0.16609861466766063 1108 2 -0.19352310631495584 1108 3 -0.1757772718347148 1108 4 -0.10487341151235796 1108 5 -0.1772602744461409 1108 6 -0.03549774517458815 1108 7 -7.57481513225855E-4 1108 8 -0.032798627762765875 1108 9 -0.009034184654865385 1108 10-11 -0.012852170826562315 1108 12-13 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0.7360211178581402 2316 74-75 0.30223682495852344 2316 76-77 -0.057682934302349054 2316 78-79 -0.26739932662085053 2316 80-81 -0.343340734553653 2316 82-83 -0.2291523978164065 2316 84-85 -0.3065432252469371 2316 86-87 -0.434701014723462 2316 88-89 -0.37987247666089985 2316 90-91 -0.44538403739405297 2316 92-93 -0.30244845508407536 2316 94-95 0.14603920638919377 2316 96-97 0.10774033870608335 2316 98-99 0.05252802090397779 2316 100-101 -0.2206956524612238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 23.0 4 81.0 5 164.0 6 342.0 7 971.0 8 2277.0 9 3788.0 10 6033.0 11 8981.0 12 12267.0 13 15707.0 14 19471.0 15 23709.0 16 29371.0 17 33888.0 18 32183.0 19 29570.0 20 30077.0 21 31483.0 22 33960.0 23 38443.0 24 46448.0 25 56552.0 26 70096.0 27 87098.0 28 107350.0 29 128651.0 30 154600.0 31 183480.0 32 219168.0 33 267374.0 34 332160.0 35 420073.0 36 521319.0 37 537805.0 38 264836.0 39 17544.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88858928829342 18.645844838279398 14.242415231557395 24.223150641869793 2 16.361358366171366 20.40387461349711 38.11416325011297 25.12060377021855 3 19.704539934622392 23.837901758400147 29.2361596827474 27.22139862423006 4 13.873609200320544 16.343384349782486 35.3405725567621 34.442433893134876 5 14.562130857603508 36.97458715642599 33.28411213514444 15.179169850826057 6 33.721847230955895 35.14300647113207 16.523670370687917 14.611475927224118 7 29.885725949852777 31.76245777200773 19.85150292765705 18.500313350482447 8 27.34568915472878 34.26597019069387 19.069891889438377 19.318448765138967 9 27.030719495708784 15.931025201554302 19.040507816243103 37.99774748649381 10-11 25.87446816021991 25.66660304983852 26.603004713927337 21.855924076014237 12-13 26.64694810432912 22.782597408733523 27.061868736806034 23.508585750131324 14-15 23.45036175324439 24.255583571144363 24.925856312147516 27.36819836346373 16-17 23.368455420078117 27.435640844257282 25.58891099411122 23.606992741553377 18-19 23.42334008521116 26.058072165903486 27.487539639964144 23.031048108921212 20-21 24.212038338915953 25.200492548204345 27.409142295626072 23.178326817253627 22-23 23.88436743416521 25.18205782477696 27.387071060881834 23.546503680175995 24-25 23.698406622809067 25.42913522099389 27.37439161382661 23.49806654237043 26-27 23.362065395812852 25.6701419783104 27.34056638430976 23.627226241566984 28-29 23.387362106120108 25.945423892081017 27.275936671042427 23.391277330756452 30-31 23.54064613194529 25.570746257325982 27.121235709195897 23.767371901532826 32-33 23.336224029194703 25.59001119912382 27.13285707194541 23.94090769973607 34-35 23.825469818045423 25.68272285622933 27.05333822293699 23.438469102788257 36-37 23.98686473899076 25.349363744187126 27.094970100088872 23.568801416733244 38-39 23.581337617501426 25.561317288693385 27.10148156384889 23.755863529956294 40-41 23.716851478920987 25.396271583998175 27.22131114107057 23.665565796010267 42-43 23.532045639501085 25.462589892212385 26.956071149789906 24.04929331849662 44-45 23.48095750604264 25.51090889790511 27.13519683791358 23.872936758138678 46-47 23.87601061767124 25.698845465749297 26.73905150628015 23.686092410299317 48-49 23.616270624603033 25.78882601157352 26.629227230753106 23.965676133070346 50-51 23.501502041668722 25.927445801158193 26.721449484491433 23.84960267268165 52-53 23.80144641683103 25.70609447532524 26.57924411770566 23.91321499013807 54-55 23.352163599134204 25.77911906973458 26.66719966553878 24.201517665592434 56-57 23.34272652018606 25.819208702007618 26.59606176534823 24.24200301245809 58-59 23.439704869460112 25.604390163678094 26.550505850272994 24.405399116588804 60-61 23.16681160897644 25.841236140223454 26.809071158710235 24.18288109208987 62-63 23.16134580057466 25.793630621941492 26.775325671087963 24.269697906395884 64-65 22.946029789272373 25.86639834793385 26.5853083282997 24.602263534494075 66-67 22.669148144209295 25.869483143677552 26.75225991704775 24.709108795065404 68-69 22.57653778734579 25.57523810793338 27.234935382605624 24.61328872211521 70-71 22.619835497145495 25.540148979593468 26.900726560049087 24.93928896321195 72-73 22.63944280323863 25.663238928243548 26.726971672090272 24.97034659642755 74-75 22.93448916020676 26.066840523929535 26.06390661624063 24.93476369962308 76-77 22.882427928216174 25.73878673156422 26.323257679412425 25.055527660807176 78-79 22.869989201425795 25.833040451650103 26.25801531337632 25.038955033547783 80-81 23.065385517378587 25.84224567524519 26.018565843472018 25.0738029639042 82-83 22.968008367697724 25.812810588466228 26.094335147741333 25.124845896094715 84-85 22.735829549264214 25.842526628282265 26.0516668440691 25.369976978384422 86-87 22.800763358069428 25.826748934217235 26.07817558536263 25.294312122350703 88-89 22.70213078837099 25.748379890522166 25.905958000052028 25.643531321054812 90-91 22.64011837581774 25.746275837474535 25.971966569900356 25.64163921680737 92-93 22.662165596810937 25.563948792920037 26.023209222105496 25.750676388163523 94-95 22.297822419440386 25.317669817850664 26.519211746627114 25.865296016081835 96-97 22.49168578796058 24.99344924779843 26.491281725352643 26.023583238888342 98-99 22.346243166270764 25.294100078251468 26.189843769185384 26.169812986292385 100-101 22.211869130012406 25.060987013373143 26.373905110724333 26.353238745890124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 496.0 1 353.0 2 261.0 3 357.0 4 343.0 5 341.0 6 555.5 7 1128.5 8 1749.5 9 1981.5 10 1868.5 11 1737.5 12 1849.5 13 2035.0 14 2254.5 15 2447.0 16 2670.0 17 2953.0 18 3254.5 19 3697.5 20 4234.0 21 4946.0 22 5792.5 23 6934.0 24 8413.0 25 10127.5 26 12477.0 27 15678.5 28 19080.5 29 23037.5 30 27909.0 31 34385.0 32 42403.5 33 46944.5 34 48939.5 35 53305.5 36 60744.0 37 69010.5 38 76332.0 39 81437.0 40 86618.0 41 93247.0 42 98442.0 43 105717.0 44 114518.0 45 122659.5 46 130808.5 47 141129.5 48 151655.5 49 155238.5 50 154805.0 51 156522.0 52 159604.0 53 163262.5 54 164762.5 55 158044.0 56 147291.0 57 135813.5 58 124669.0 59 111253.5 60 93570.0 61 77488.5 62 62878.0 63 47459.5 64 34111.0 65 24693.0 66 17920.5 67 13777.5 68 10274.5 69 7267.0 70 5374.0 71 3721.5 72 2540.0 73 1954.0 74 1411.5 75 990.5 76 673.5 77 367.0 78 221.0 79 140.5 80 93.0 81 58.5 82 31.5 83 21.0 84 16.0 85 7.0 86 4.0 87 2.5 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022588840369628813 2 0.023385156716384234 3 5.3087756450361485E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1852653870216894E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.963163467554223E-5 26-27 2.919826604769882E-4 28-29 6.635969556295186E-5 30-31 5.441495036162052E-4 32-33 0.0023358612838159055 34-35 0.004525731237393317 36-37 0.10727708384706798 38-39 0.09880958669323532 40-41 0.10918824307928097 42-43 0.11040926147763931 44-45 0.10305660720926423 46-47 0.09612865499249205 48-49 0.09737621726907555 50-51 0.09652681316586978 52-53 0.0762340182627191 54-55 0.06598808126779933 56-57 0.03502464731812599 58-59 0.01251543858317272 60-61 0.008786023692534827 62-63 0.0023225893447033153 64-65 0.004923889410771028 66-67 0.16316521945018603 68-69 0.4359035682139182 70-71 0.412969657427362 72-73 0.1921511344720834 74-75 0.02757908947596279 76-77 0.032011917139567976 78-79 0.004857529715208076 80-81 0.001393553606821989 82-83 6.635969556295186E-5 84-85 4.7778980805325343E-4 86-87 0.001207746459245724 88-89 2.3889490402662672E-4 90-91 6.237811382917474E-4 92-93 0.0025083964922795804 94-95 0.08229929443717289 96-97 0.01603250244800917 98-99 0.01710752951612899 100-101 0.009462892587276936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3767347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.83712646284252 #Duplication Level Percentage of deduplicated Percentage of total 1 65.8462493466135 25.5727911297829 2 14.701381346110526 11.419188130347369 3 6.6465273104775635 7.743960650872611 4 3.6834025773171986 5.722110868353123 5 2.1881295658045055 4.249033233211713 6 1.460776597447222 3.4039419269411133 7 0.9929764924569228 2.6995047528525506 8 0.7441948579238234 2.3121911848147723 9 0.558472270413268 1.9520512372827787 >10 2.87502179536677 19.975928854469018 >50 0.19751001572147725 5.281653046817637 >100 0.09844230754536715 6.511392018866895 >500 0.004587521053459863 1.2514974353679977 >1k 0.002191054822133889 1.4858257862016773 >5k 1.3694092638336807E-4 0.41892974381782955 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9065 0.24062025611126342 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6689 0.177552001448234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4813 0.1277556858977949 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0617551290072297E-4 2 0.0 0.0 0.0 0.0 1.0617551290072297E-4 3 0.0 0.0 0.0 0.0 1.0617551290072297E-4 4 0.0 0.0 0.0 0.0 1.0617551290072297E-4 5 0.0 0.0 0.0 2.6543878225180743E-5 1.3271939112590372E-4 6 0.0 0.0 0.0 2.6543878225180743E-5 1.8580714757626522E-4 7 2.6543878225180743E-5 0.0 0.0 2.6543878225180743E-5 2.6543878225180744E-4 8 2.6543878225180743E-5 0.0 0.0 2.6543878225180743E-5 2.6543878225180744E-4 9 2.6543878225180743E-5 0.0 0.0 5.3087756450361485E-5 2.6543878225180744E-4 10-11 2.6543878225180743E-5 0.0 0.0 7.963163467554223E-5 3.1852653870216894E-4 12-13 5.3087756450361485E-5 0.0 0.0 1.7253520846367484E-4 8.095882858680126E-4 14-15 5.3087756450361485E-5 0.0 0.0 1.8580714757626522E-4 0.0010219393116694586 16-17 5.3087756450361485E-5 0.0 0.0 1.990790866888556E-4 0.0012342903374709046 18-19 5.3087756450361485E-5 0.0 0.0 2.6543878225180744E-4 0.0013271939112590371 20-21 5.3087756450361485E-5 0.0 0.0 3.716142951525304E-4 0.0015528168761730736 22-23 5.3087756450361485E-5 0.0 0.0 5.972372600665667E-4 0.0016589923890737964 24-25 7.963163467554223E-5 0.0 0.0 0.0010219393116694586 0.0016722643281863868 26-27 7.963163467554223E-5 0.0 0.0 0.002362405162041086 0.0019111592322130135 28-29 7.963163467554223E-5 0.0 0.0 0.008746207875197056 0.001990790866888556 30-31 7.963163467554223E-5 0.0 0.0 0.03149431151417695 0.002004062806001146 32-33 9.29035737881326E-5 0.0 0.0 0.08268418067143801 0.0020969663797892786 34-35 1.459913302384941E-4 0.0 0.0 0.1570070397019441 0.0022695015882529535 36-37 1.8580714757626522E-4 0.0 0.0 0.2785381861559342 0.0023225893447033153 38-39 2.1235102580144594E-4 0.0 0.0 0.4715254527921108 0.002388949040266267 40-41 2.1235102580144594E-4 0.0 0.0 0.6935649941457477 0.002388949040266267 42-43 2.388949040266267E-4 0.0 0.0 0.9138393675974101 0.002388949040266267 44-45 2.6543878225180744E-4 0.0 0.0 1.1766768497831497 0.002415492918491448 46-47 3.450704169273497E-4 0.0 0.0 1.4788656314377198 0.00249512455316699 48-49 3.7161429515253044E-4 0.0 0.0 1.799104250285413 0.002614572005180303 50-51 3.9815817337771113E-4 0.0 0.0 2.147890810164288 0.002680931700743255 52-53 3.9815817337771113E-4 0.0 0.0 2.4802467094217757 0.00284019497009434 54-55 3.9815817337771113E-4 0.0 0.0 2.832019455600984 0.0028667388483195203 56-57 4.247020516028919E-4 0.0 0.0 3.2234354839094994 0.002959642422107653 58-59 4.247020516028919E-4 0.0 0.0 3.6109893779362507 0.0030658179350083756 60-61 4.247020516028919E-4 0.0 0.0 4.010196034503856 0.0031321776305713277 62-63 4.247020516028919E-4 0.0 0.0 4.396568725949587 0.0033710725345979546 64-65 4.3797399071548223E-4 0.0 0.0 4.801415956640044 0.0034772480474986774 66-67 4.5124592982807263E-4 0.0 0.0 5.220955223928138 0.0035568796821742197 68-69 4.5124592982807263E-4 0.0 0.0 5.69366187930127 0.003596695499511991 70-71 4.5124592982807263E-4 0.0 0.0 6.220610949827558 0.0037294148906378945 72-73 4.5124592982807263E-4 0.0 0.0 6.776293768532604 0.004008125612002292 74-75 4.5124592982807263E-4 0.0 0.0 7.3336223076876115 0.00426029245514151 76-77 4.5124592982807263E-4 0.0 0.0 7.9223920706003454 0.004353196028929642 78-79 4.5124592982807263E-4 0.0 0.0 8.565191897640435 0.004366467968042232 80-81 5.574214427287956E-4 0.0 0.0 9.247263392514679 0.004499187359168136 82-83 5.574214427287956E-4 0.0 0.0 9.927224649070022 0.004724810324082172 84-85 5.574214427287956E-4 0.0 0.0 10.606363045400384 0.004804441958757714 86-87 5.706933818413859E-4 0.0 0.0 11.279210542591379 0.004976977167221389 88-89 5.839653209539764E-4 0.0 0.0 11.983154193123172 0.0051893281930228356 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14385 0.0 30.64608 1 GGTATCA 9505 0.0 30.454176 1 TATCAAC 17485 0.0 24.936659 2 TCAACGC 17455 0.0 24.837349 4 ATCAACG 17470 0.0 24.81602 3 GTACATG 15530 0.0 24.658756 1 CAACGCA 17665 0.0 24.542082 5 TACATGG 15640 0.0 24.329098 2 AACGCAG 18480 0.0 23.511066 6 ACATGGG 16830 0.0 22.011303 3 GTATAGG 720 0.0 21.090487 1 ACGCAGA 20720 0.0 20.831978 7 CGCAGAG 21065 0.0 20.35569 8 AGAGTAC 16475 0.0 19.664234 10-11 GTACTTT 9095 0.0 19.531416 14-15 GAGTACT 9080 0.0 19.511444 12-13 GTAAGGT 1710 0.0 19.41694 4 TAAGGTG 1875 0.0 18.973124 5 TAAGGTA 1080 0.0 18.885286 4 CATGGGA 7940 0.0 18.698324 4 >>END_MODULE