FastQCFastQC Report
Fri 27 May 2016
SRR939857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101217
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.3521768575068639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56790.2702719424029027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.1803716608041911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.050.9162331
GTATCAA74200.042.6490551
TATCAAC98650.032.703882
TCAACGC99000.032.6872254
ATCAACG98950.032.5598873
CAACGCA101600.031.7113915
AACGCAG105850.030.707116
ACGCAGA117300.027.1433687
CGCAGAG118450.026.7590248
GCAGAGT126700.023.8551049
TACATGG84400.022.5980572
GTACATG83400.022.2432461
TGGTATC20300.022.2031732
GAGTACT71900.022.03993212-13
GTGGTAT20950.021.9672241
ACATGGG85300.021.0267263
AGAGTAC117800.020.7414510-11
GTACTTT76450.020.57501214-15
AGTACTT76000.020.5075812-13
CAGAGTA122950.020.29711510-11
Produced by FastQC (version 0.11.5)