FastQCFastQC Report
Fri 27 May 2016
SRR939857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101217
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80860.3848246040270948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.3355198439761338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.226345018149006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.051.395191
GTATCAA77250.040.0834051
ATCAACG98800.031.2369253
TATCAAC100550.030.740862
TCAACGC100600.030.631184
CAACGCA102700.030.0048335
AACGCAG106500.028.8748726
ACGCAGA121750.025.1807427
CGCAGAG121700.024.9976088
GTGGTAT22100.024.3114191
TGGTATC22600.023.1424242
GTACATG87400.022.3786741
GCAGAGT133750.021.9356659
TACATGG87250.021.8177622
ACATGGG85350.021.3644333
GAGTACT74500.020.9866712-13
GTACTTT78550.019.96479814-15
AGTACTT78150.019.73531212-13
CATGGGG40250.019.6665124
AGAGTAC125250.019.62679110-11
Produced by FastQC (version 0.11.5)