##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2101217 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40232208286912 31.0 31.0 34.0 30.0 34.0 2 31.61601633719887 31.0 31.0 34.0 30.0 34.0 3 31.668982308823885 31.0 31.0 34.0 30.0 34.0 4 35.48545295416894 37.0 35.0 37.0 33.0 37.0 5 35.21485120289813 37.0 35.0 37.0 32.0 37.0 6 35.24545108858342 37.0 35.0 37.0 32.0 37.0 7 35.166471620970135 37.0 35.0 37.0 32.0 37.0 8 35.103636130870825 37.0 35.0 37.0 32.0 37.0 9 36.699688323481105 39.0 35.0 39.0 32.0 39.0 10-11 36.55336788156578 38.0 35.0 39.0 32.0 39.0 12-13 36.49406915135371 38.0 35.0 39.0 32.0 39.0 14-15 37.52077819663557 39.0 36.0 41.0 32.0 41.0 16-17 37.447273889369825 39.0 36.0 41.0 32.0 41.0 18-19 37.43780675675097 39.0 36.0 41.0 32.0 41.0 20-21 37.35902051049463 39.0 36.0 41.0 31.5 41.0 22-23 37.22072541769841 39.0 36.0 40.0 31.0 41.0 24-25 37.06513796528393 39.0 36.0 40.0 31.0 41.0 26-27 36.771541206833945 39.0 35.5 40.0 30.5 41.0 28-29 36.5336947587993 38.0 35.0 40.0 30.0 41.0 30-31 36.27062626087643 38.0 35.0 40.0 30.0 41.0 32-33 36.22481542839222 38.0 35.0 40.0 30.0 41.0 34-35 36.29555871668657 38.0 35.0 40.0 30.0 41.0 36-37 36.25376841135399 38.0 35.0 40.0 30.0 41.0 38-39 36.11740814965803 38.0 35.0 40.0 30.0 41.0 40-41 35.9381946272089 38.0 35.0 40.0 29.0 41.0 42-43 35.72543483133822 38.0 34.5 40.0 28.5 41.0 44-45 35.460541676561725 38.0 34.0 40.0 27.0 41.0 46-47 35.148248610210175 38.0 34.0 40.0 26.5 41.0 48-49 34.83062268199809 38.0 33.5 40.0 26.0 41.0 50-51 34.55609177919273 38.0 33.0 40.0 24.5 41.0 52-53 34.27001661418121 38.0 33.0 40.0 24.0 41.0 54-55 33.960497416497205 37.0 33.0 40.0 23.5 41.0 56-57 32.723175664388776 36.5 31.5 39.5 16.0 41.0 58-59 29.51012127733594 34.5 24.5 38.5 7.0 40.0 60-61 29.530872822749863 34.0 25.0 38.0 9.0 40.0 62-63 31.22671052061734 35.0 29.5 38.0 8.5 40.0 64-65 30.697746591618095 35.0 29.0 38.0 4.0 40.0 66-67 25.234860083465914 28.5 18.0 36.0 2.0 39.0 68-69 17.527216132365197 13.0 7.0 29.0 2.0 35.5 70-71 16.38275032992785 14.5 7.5 25.5 2.0 33.5 72-73 18.528592477597506 18.5 7.5 30.0 2.0 34.5 74-75 19.291490836025027 18.5 7.5 30.5 2.0 35.0 76-77 20.37444228749339 23.5 8.5 31.5 2.0 34.5 78-79 23.54086013010555 29.0 12.0 34.0 2.0 36.0 80-81 26.139514386186672 31.5 22.5 35.0 2.0 36.0 82-83 26.99325914458145 33.0 26.0 35.0 2.0 36.0 84-85 26.940679139755673 33.0 26.0 35.0 2.0 36.0 86-87 26.630560051627224 33.0 25.0 35.0 2.0 35.5 88-89 26.181476020801277 33.0 24.5 35.0 2.0 35.0 90-91 24.658609034668956 31.5 11.0 34.5 2.0 35.0 92-93 22.639694043975467 31.0 2.0 34.0 2.0 35.0 94-95 18.13452465880487 23.0 2.0 33.0 2.0 35.0 96-97 16.792795556099158 15.0 2.0 32.5 2.0 34.5 98-99 15.66565090611774 2.0 2.0 32.0 2.0 34.0 100-101 15.034211126218757 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4131562573642569 1101 2 0.4143205560039256 1101 3 0.42298545214571703 1101 4 0.391923281184809 1101 5 0.33164918176648683 1101 6 0.48691767921850726 1101 7 0.4756954287924131 1101 8 0.38210008546653285 1101 9 0.5300199128792826 1101 10-11 0.5026562070187879 1101 12-13 0.5892898596221201 1101 14-15 0.5371007788935813 1101 16-17 0.5032143561481206 1101 18-19 0.5914346065651799 1101 20-21 0.6329219513101734 1101 22-23 0.6711106523793475 1101 24-25 0.5253548217319164 1101 26-27 0.7231132486776417 1101 28-29 0.5914700943258282 1101 30-31 0.6359392194977431 1101 32-33 0.6216806132153536 1101 34-35 0.6083297121666931 1101 36-37 0.6735427920225092 1101 38-39 0.49391916673827296 1101 40-41 0.5148728191765954 1101 42-43 0.44025952915504973 1101 44-45 0.33547621507927516 1101 46-47 -0.0034254557140869224 1101 48-49 0.2530502508424135 1101 50-51 0.1188651869876054 1101 52-53 0.1994720800116312 1101 54-55 0.05075237339163863 1101 56-57 0.9170724512161854 1101 58-59 -8.528283592054596 1101 60-61 -9.47043089161885 1101 62-63 -2.0992744524248685 1101 64-65 0.4461286492970373 1101 66-67 -1.3174982796118044 1101 68-69 -3.9003152023437586 1101 70-71 -4.414366637501217 1101 72-73 -6.859702546964186 1101 74-75 -7.645154170386963 1101 76-77 -8.880714253569913 1101 78-79 -11.89076473404323 1101 80-81 -7.765595518390121 1101 82-83 -3.835871075686203 1101 84-85 -2.8453866567563857 1101 86-87 -2.678574701522468 1101 88-89 -2.8105055094577587 1101 90-91 -8.451017230107597 1101 92-93 -13.67170746009573 1101 94-95 -12.112477437238304 1101 96-97 -12.41876757503994 1101 98-99 -11.975320641073107 1101 100-101 -11.576964576165793 1102 1 0.5487674124275479 1102 2 0.5666939737254459 1102 3 0.534842651512804 1102 4 0.43611829700759586 1102 5 0.5134727577158529 1102 6 0.4140636444083796 1102 7 0.5243801598050695 1102 8 0.5857096582513464 1102 9 0.6945274287020666 1102 10-11 0.7823387623985383 1102 12-13 0.8218205162676853 1102 14-15 0.8491162061087678 1102 16-17 0.8860367453886226 1102 18-19 0.8138732932740425 1102 20-21 0.7871347677658704 1102 22-23 0.8168038881388426 1102 24-25 0.8783512616053386 1102 26-27 1.035909925892831 1102 28-29 0.8565116291359516 1102 30-31 0.8844409995610363 1102 32-33 0.8311198932786468 1102 34-35 0.7131606061540339 1102 36-37 0.6550993584782034 1102 38-39 0.5260344752825787 1102 40-41 0.5875389584170989 1102 42-43 0.6237889990917509 1102 44-45 0.5187633986235838 1102 46-47 0.6004709050454053 1102 48-49 0.8604721258424135 1102 50-51 0.8240372597724104 1102 52-53 0.7797628236825176 1102 54-55 0.7944183148473272 1102 56-57 1.5431157660896062 1102 58-59 -9.12239454933308 1102 60-61 -10.038201856808723 1102 62-63 -3.10812037805778 1102 64-65 0.052180864486910394 1102 66-67 -1.1452821008143346 1102 68-69 -4.07125566911591 1102 70-71 -4.418653623260713 1102 72-73 -6.918988543799628 1102 74-75 -8.011318133994557 1102 76-77 -8.872387513063583 1102 78-79 -11.721352154929306 1102 80-81 -7.9565369740863225 1102 82-83 -4.0487073098634205 1102 84-85 -3.1127027168829713 1102 86-87 -2.8592709040541173 1102 88-89 -3.042507091736237 1102 90-91 -8.663482617765824 1102 92-93 -14.762564857247629 1102 94-95 -12.50607909863071 1102 96-97 -12.207305945293104 1102 98-99 -11.729632349933865 1102 100-101 -11.513347091988578 1103 1 0.2710881477869087 1103 2 0.29208217248321233 1103 3 0.25626637045690615 1103 4 0.17014877061007638 1103 5 0.13052993384717837 1103 6 0.18515878083251636 1103 7 0.2090386835406548 1103 8 0.20885392954681947 1103 9 0.2988177181874292 1103 10-11 0.34528969684839694 1103 12-13 0.2966392413120573 1103 14-15 0.4589050537130319 1103 16-17 0.42370380949687814 1103 18-19 0.3340672540105132 1103 20-21 0.31100760494421564 1103 22-23 0.2663155202373346 1103 24-25 0.33949325916523065 1103 26-27 0.20897031852814507 1103 28-29 0.37875472474375727 1103 30-31 0.33138220131348106 1103 32-33 0.425763790239607 1103 34-35 0.3245206189091405 1103 36-37 -0.03263286756438788 1103 38-39 0.023408934577169305 1103 40-41 0.14853884750316837 1103 42-43 0.1751071418379837 1103 44-45 0.08651655967060634 1103 46-47 -0.16820284162717059 1103 48-49 -0.15478027267133854 1103 50-51 -0.01117999288952376 1103 52-53 -0.11312690014179339 1103 54-55 -0.12808300225559321 1103 56-57 0.36447397649332913 1103 58-59 -7.02812845294887 1103 60-61 -8.997531798024344 1103 62-63 -2.9590946876185598 1103 64-65 -0.8269035410144205 1103 66-67 -2.2223523786537314 1103 68-69 -4.326855782802692 1103 70-71 -4.841869642005166 1103 72-73 -7.730521512965556 1103 74-75 -8.255124630778052 1103 76-77 -9.178084329833771 1103 78-79 -12.281025929330372 1103 80-81 -8.725694582781976 1103 82-83 -5.176711690963685 1103 84-85 -4.329495779216597 1103 86-87 -4.064450584621994 1103 88-89 -4.096231026962503 1103 90-91 -9.60660560745083 1103 92-93 -14.75388064307283 1103 94-95 -12.77302605404331 1103 96-97 -12.482815456162669 1103 98-99 -12.109465424468027 1103 100-101 -11.731964688483698 1104 1 0.10646355604457014 1104 2 0.13534898892301683 1104 3 0.08978220047068675 1104 4 0.14395420397676162 1104 5 0.07689478876231703 1104 6 0.14749524178137108 1104 7 0.12625441349590716 1104 8 0.16752787090874932 1104 9 0.1688832282027164 1104 10-11 0.13256621707721195 1104 12-13 0.10455991189295588 1104 14-15 0.1275414259307368 1104 16-17 0.24000202165871087 1104 18-19 0.2536050968997472 1104 20-21 0.23042273454831985 1104 22-23 0.29187292173632073 1104 24-25 0.34252333335957275 1104 26-27 0.41643600600572483 1104 28-29 0.19334911013899614 1104 30-31 0.34059041743989127 1104 32-33 0.2783237781570662 1104 34-35 0.30020450948664745 1104 36-37 0.34526741373222336 1104 38-39 0.35025576951836257 1104 40-41 0.35109600357558435 1104 42-43 0.3503228340353033 1104 44-45 0.18255858425101934 1104 46-47 0.3239151896741532 1104 48-49 0.45002159257060015 1104 50-51 0.4879610141145747 1104 52-53 0.37237877298776567 1104 54-55 0.2303200259307019 1104 56-57 1.109268101358822 1104 58-59 -6.087568880368686 1104 60-61 -8.158201246192139 1104 62-63 -2.404199371490261 1104 64-65 -0.05954433091039846 1104 66-67 -1.483443771732702 1104 68-69 -4.2188303339552515 1104 70-71 -4.957621308074 1104 72-73 -7.818686288589033 1104 74-75 -8.492480442668027 1104 76-77 -9.124529420358417 1104 78-79 -12.24128098417377 1104 80-81 -8.395992304090665 1104 82-83 -4.585875541517783 1104 84-85 -3.70836360756903 1104 86-87 -3.5423527538152975 1104 88-89 -3.737327706152218 1104 90-91 -9.13082260229687 1104 92-93 -14.878599818439552 1104 94-95 -12.442365667779645 1104 96-97 -12.174790951263578 1104 98-99 -11.70961362435853 1104 100-101 -11.255667231480546 1105 1 0.18878087216537764 1105 2 0.17816401117863023 1105 3 0.15487912935300585 1105 4 0.08490594525977713 1105 5 0.13268507044993783 1105 6 0.11310488192398793 1105 7 0.09012122566025482 1105 8 0.13739212547980628 1105 9 0.09893890654226567 1105 10-11 0.29646319669940624 1105 12-13 0.2114443263488326 1105 14-15 0.3259722844483477 1105 16-17 0.30425518327876944 1105 18-19 0.3005432761080016 1105 20-21 0.2366451454187981 1105 22-23 0.19224523099152435 1105 24-25 0.27053193420209 1105 26-27 0.2835983622224205 1105 28-29 0.16729600023458602 1105 30-31 0.1673378667582881 1105 32-33 0.1581510457443116 1105 34-35 0.34455934601046323 1105 36-37 0.18183839561927329 1105 38-39 0.20737762056972286 1105 40-41 0.23632563132596118 1105 42-43 0.1739201827684056 1105 44-45 0.40169684119536697 1105 46-47 0.3579088732101994 1105 48-49 0.2984622358611375 1105 50-51 0.2740479885489435 1105 52-53 0.27355866481859437 1105 54-55 0.11920715301837248 1105 56-57 0.7197558058836151 1105 58-59 -6.8142996874416895 1105 60-61 -8.844277270354294 1105 62-63 -2.6718439462537837 1105 64-65 -0.24213483463918095 1105 66-67 -1.4109514789354343 1105 68-69 -3.815916152105924 1105 70-71 -4.721393850320638 1105 72-73 -6.2571547032877675 1105 74-75 -6.540526252752361 1105 76-77 -8.542134313937492 1105 78-79 -11.739053660859394 1105 80-81 -7.8801753948104185 1105 82-83 -3.696649335456428 1105 84-85 -2.7642232771201733 1105 86-87 -2.4778258353899467 1105 88-89 -2.6125172353067754 1105 90-91 -8.130559416298908 1105 92-93 -10.947906910150252 1105 94-95 -11.305588500940322 1105 96-97 -11.659374648476625 1105 98-99 -11.137751107736612 1105 100-101 -10.829716278942385 1106 1 0.13170946644764925 1106 2 0.16512362196665364 1106 3 0.22300612513089746 1106 4 0.17846675806286783 1106 5 0.20730912706258664 1106 6 0.12561750747370581 1106 7 0.19428044246838994 1106 8 0.13349153639205014 1106 9 0.17020660583774827 1106 10-11 0.25964129066486663 1106 12-13 0.32694967769985084 1106 14-15 0.479490735003246 1106 16-17 0.1855381587052065 1106 18-19 0.3664290407614814 1106 20-21 0.296894503946767 1106 22-23 0.39896272921924236 1106 24-25 0.5716026749219196 1106 26-27 0.31531476257625 1106 28-29 0.4018908690857046 1106 30-31 0.4587637853650577 1106 32-33 0.564794123806287 1106 34-35 0.4560135427118226 1106 36-37 0.6478168521967902 1106 38-39 0.5099089256594667 1106 40-41 0.602661445854288 1106 42-43 0.662975164292753 1106 44-45 0.787709692593431 1106 46-47 0.7564617970052083 1106 48-49 0.5146708538574885 1106 50-51 0.3492139243452783 1106 52-53 0.3922039354915583 1106 54-55 0.3312450265307447 1106 56-57 0.9708579928484014 1106 58-59 -6.436086053730062 1106 60-61 -9.08337695102983 1106 62-63 -2.4540904628567723 1106 64-65 0.01981591473816735 1106 66-67 -1.3506625185279049 1106 68-69 -4.038021137080737 1106 70-71 -4.854844342481819 1106 72-73 -6.376765710885056 1106 74-75 -6.701619248944306 1106 76-77 -8.678142852259565 1106 78-79 -12.247620213974534 1106 80-81 -8.031803933885318 1106 82-83 -4.1671039807176875 1106 84-85 -3.150897597536236 1106 86-87 -2.9524241703857754 1106 88-89 -3.0766622424900056 1106 90-91 -8.180503267891453 1106 92-93 -11.319225111644613 1106 94-95 -11.35312291144478 1106 96-97 -11.808055788257928 1106 98-99 -11.536651822295676 1106 100-101 -11.263005540481041 1107 1 0.12097948997018904 1107 2 0.11035316513416404 1107 3 0.09870132570737411 1107 4 0.11731450278775668 1107 5 0.19031163168932608 1107 6 0.1317076549895546 1107 7 0.20974270323526412 1107 8 0.23802478380409298 1107 9 0.09392976998444169 1107 10-11 0.2850109532545062 1107 12-13 0.19756598570862138 1107 14-15 0.26116660487693366 1107 16-17 0.2838538845231824 1107 18-19 0.20578096462590878 1107 20-21 0.25634966827123407 1107 22-23 0.3098883697402286 1107 24-25 0.34205990421430954 1107 26-27 0.39186479797115936 1107 28-29 0.41063305601529976 1107 30-31 0.2740372467972989 1107 32-33 0.4454624686892643 1107 34-35 0.31055849584134165 1107 36-37 0.2833492202912211 1107 38-39 0.4552592956394932 1107 40-41 0.458809883875098 1107 42-43 0.5360133654057151 1107 44-45 0.47412998895838854 1107 46-47 0.5309618636682742 1107 48-49 0.39873881685630863 1107 50-51 0.39120649934916685 1107 52-53 0.4398114852428421 1107 54-55 0.2869406715276881 1107 56-57 0.8311796183479743 1107 58-59 -6.877142012614819 1107 60-61 -9.265331416979286 1107 62-63 -2.72354278606473 1107 64-65 -0.08114391441390723 1107 66-67 -1.5255364142224224 1107 68-69 -4.007623769637075 1107 70-71 -4.767610558273347 1107 72-73 -6.461513121342268 1107 74-75 -7.1630620044146465 1107 76-77 -9.425014052861435 1107 78-79 -12.56410868919968 1107 80-81 -8.383141917232248 1107 82-83 -4.172821926098418 1107 84-85 -3.084509116914557 1107 86-87 -2.8419856861134996 1107 88-89 -2.951175364004083 1107 90-91 -8.48613121812906 1107 92-93 -12.157548916391658 1107 94-95 -12.040637196388133 1107 96-97 -12.466974592639914 1107 98-99 -11.859541936162547 1107 100-101 -11.318227560245052 1108 1 0.06851417966892726 1108 2 0.05634914613924025 1108 3 0.05302691875418475 1108 4 -0.021182349544133672 1108 5 -0.0927233629737998 1108 6 -0.04528441593644317 1108 7 0.03254287532231359 1108 8 0.0734521151478944 1108 9 0.05872462697792713 1108 10-11 0.05937270636405145 1108 12-13 -0.06558519493921011 1108 14-15 0.08583495610876923 1108 16-17 0.08245377125069808 1108 18-19 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30-31 -0.3276683754389609 2312 32-33 -0.2543328788355268 2312 34-35 -0.42067607959460673 2312 36-37 -0.31531541306090105 2312 38-39 -0.2894413651084733 2312 40-41 -0.2942482122227972 2312 42-43 -0.273176999705818 2312 44-45 -0.3160618575453853 2312 46-47 -0.3828500119349343 2312 48-49 -0.3399547679781634 2312 50-51 -0.3393367861288823 2312 52-53 -0.19912643551865727 2312 54-55 -0.3757626698348062 2312 56-57 0.9410044959620478 2312 58-59 4.23381379053518 2312 60-61 4.046489760192532 2312 62-63 1.5254568712625947 2312 64-65 1.3756739113292369 2312 66-67 -0.07848555256046552 2312 68-69 -0.9236155930494121 2312 70-71 0.10715327631059779 2312 72-73 7.116076217283599 2312 74-75 5.678377109888753 2312 76-77 5.81025769291718 2312 78-79 5.819367274702646 2312 80-81 3.382028218301798 2312 82-83 2.0821015828592913 2312 84-85 1.7013130584202507 2312 86-87 1.5410480001700577 2312 88-89 1.6939852231947548 2312 90-91 3.205989001065209 2312 92-93 5.302839098994529 2312 94-95 9.167870412557079 2312 96-97 9.77364900295031 2312 98-99 10.27606740958098 2312 100-101 9.481929139190845 2313 1 -0.31552955906277447 2313 2 -0.3901452605273299 2313 3 -0.3904033988165452 2313 4 -0.3304777172987059 2313 5 -0.4166701766192631 2313 6 -0.3830631953404833 2313 7 -0.4847391408330495 2313 8 -0.42817176157162606 2313 9 -0.40229257181254496 2313 10-11 -0.5416746240259158 2313 12-13 -0.5300952991398873 2313 14-15 -0.603069943026675 2313 16-17 -0.6831085433104249 2313 18-19 -0.6984334044410687 2313 20-21 -0.7072347001221502 2313 22-23 -0.6436728705581558 2313 24-25 -0.6904157850187858 2313 26-27 -0.6781474279564961 2313 28-29 -0.7098901179821979 2313 30-31 -0.8912747978350097 2313 32-33 -0.666211666776924 2313 34-35 -0.6995045531193327 2313 36-37 -0.8122241928803788 2313 38-39 -1.0547415964032965 2313 40-41 -1.2027386764120465 2313 42-43 -0.7776633411202667 2313 44-45 -0.8652790313887735 2313 46-47 -0.6925399790632767 2313 48-49 -0.9769312230151357 2313 50-51 -0.7368729013020996 2313 52-53 -0.7721582986929434 2313 54-55 -0.7947562747471508 2313 56-57 0.2727561466474526 2313 58-59 3.99735042673732 2313 60-61 3.725455705459698 2313 62-63 0.921167491178533 2313 64-65 0.772885115207373 2313 66-67 1.2762456948320207 2313 68-69 -0.17226158075650488 2313 70-71 2.4230599484251663 2313 72-73 7.856634462066985 2313 74-75 9.59053431943278 2313 76-77 8.351141331110973 2313 78-79 5.847940442332941 2313 80-81 2.8780876142216236 2313 82-83 1.5993802518903877 2313 84-85 1.1646043667934372 2313 86-87 0.9909092112196589 2313 88-89 1.0303559691941864 2313 90-91 2.5651703451304293 2313 92-93 4.497856901963981 2313 94-95 8.321090968783864 2313 96-97 9.050885118416241 2313 98-99 9.499395799510348 2313 100-101 8.54153564010282 2314 1 -0.3177142327471607 2314 2 -0.21568955372879728 2314 3 -0.2637339395853644 2314 4 -0.28077412187759876 2314 5 -0.3431386191560222 2314 6 -0.3737470357080923 2314 7 -0.33137717172571257 2314 8 -0.40120147943584783 2314 9 -0.3205061091515091 2314 10-11 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2314 76-77 8.85967623485655 2314 78-79 6.583310646651395 2314 80-81 3.5547619027855575 2314 82-83 2.112583767507626 2314 84-85 1.6335132252967277 2314 86-87 1.5831791791405614 2314 88-89 1.7683030164168407 2314 90-91 2.987015900329016 2314 92-93 4.9077144948738365 2314 94-95 8.755774842717045 2314 96-97 9.552135350463969 2314 98-99 9.84852384806946 2314 100-101 9.168320181305928 2315 1 -0.7004513375724493 2315 2 -0.53922707890613 2315 3 -0.5646228090135104 2315 4 -0.44023861088714256 2315 5 -0.8316752685999305 2315 6 -0.6846068599775847 2315 7 -0.8398331077387908 2315 8 -0.8920726882398853 2315 9 -1.1192472423505677 2315 10-11 -0.9340332222505907 2315 12-13 -0.6651655034691544 2315 14-15 -0.9040773245929827 2315 16-17 -0.97472257478681 2315 18-19 -0.9549178251470067 2315 20-21 -1.0414562410060668 2315 22-23 -0.9334085570365929 2315 24-25 -0.9303247252367655 2315 26-27 -1.0674069600720841 2315 28-29 -1.0762597524429935 2315 30-31 -1.0379753929828155 2315 32-33 -0.8590322448792449 2315 34-35 -0.9761417513021016 2315 36-37 -1.212458207311272 2315 38-39 -1.174465798840231 2315 40-41 -0.9045114801793872 2315 42-43 -1.0213521741538614 2315 44-45 -1.0346631364641397 2315 46-47 -0.8741727353054713 2315 48-49 -0.9763357140698687 2315 50-51 -0.9338163586486417 2315 52-53 -0.863671935089414 2315 54-55 -0.8753802048895096 2315 56-57 0.4199455358264501 2315 58-59 3.9254056151406047 2315 60-61 3.4662252265246103 2315 62-63 0.9714506197492376 2315 64-65 0.9077181451886673 2315 66-67 1.8445547961154887 2315 68-69 6.001311490971808 2315 70-71 5.134889414019987 2315 72-73 9.00443525006002 2315 74-75 10.027847162058073 2315 76-77 8.846828495708348 2315 78-79 6.253688963491744 2315 80-81 3.172903815387361 2315 82-83 1.5852565059260577 2315 84-85 1.0379688839942212 2315 86-87 0.9650712012090388 2315 88-89 1.1795872065093782 2315 90-91 2.259536844953473 2315 92-93 4.390292873015529 2315 94-95 8.611889651369289 2315 96-97 9.182373331022683 2315 98-99 9.374479492171398 2315 100-101 8.351907842221946 2316 1 -0.40346365067711076 2316 2 -0.4263689110590718 2316 3 -0.5915542537202612 2316 4 -0.36749524191061056 2316 5 -0.5829968443070115 2316 6 -0.42666236152829384 2316 7 -0.5662565211535906 2316 8 -0.6469111366035349 2316 9 -0.738523500277708 2316 10-11 -0.6488772253982944 2316 12-13 -0.6595933607112059 2316 14-15 -0.7295124493441776 2316 16-17 -0.9258537834152492 2316 18-19 -0.7749700169722189 2316 20-21 -0.57149952203185 2316 22-23 -0.675931586245504 2316 24-25 -2.0875025477614173 2316 26-27 -1.4302084228318819 2316 28-29 -1.0628368482167332 2316 30-31 -0.7383983666438851 2316 32-33 -0.7194005964970742 2316 34-35 -0.6561167547738407 2316 36-37 -0.6209732283731597 2316 38-39 -1.0091681549944624 2316 40-41 -0.7998593485310082 2316 42-43 -1.02505767801906 2316 44-45 -0.9252518278495927 2316 46-47 -0.7937394621489631 2316 48-49 -1.083471297090746 2316 50-51 -1.1446996850384892 2316 52-53 -1.1173189979973444 2316 54-55 -0.9968968923866157 2316 56-57 0.21609942648977665 2316 58-59 3.7155146277777433 2316 60-61 3.5279116821094796 2316 62-63 1.0866036077601642 2316 64-65 0.8710490483039734 2316 66-67 6.040518319700176 2316 68-69 8.818659197308893 2316 70-71 5.482807527245004 2316 72-73 9.179025006112418 2316 74-75 10.144704774426724 2316 76-77 8.78421566415717 2316 78-79 6.3731584816142295 2316 80-81 3.2414099256498226 2316 82-83 1.8679359948859222 2316 84-85 1.3855334583588927 2316 86-87 1.4216327898772825 2316 88-89 1.3802474157395785 2316 90-91 2.937828054507701 2316 92-93 4.847876889124404 2316 94-95 8.777632835186353 2316 96-97 9.674998776562655 2316 98-99 9.884832799582407 2316 100-101 8.784586752558473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 20.0 4 41.0 5 91.0 6 239.0 7 553.0 8 1267.0 9 2214.0 10 3671.0 11 5603.0 12 7750.0 13 10316.0 14 13723.0 15 19103.0 16 25681.0 17 32884.0 18 35484.0 19 35235.0 20 36925.0 21 36983.0 22 35254.0 23 34413.0 24 35986.0 25 38973.0 26 51340.0 27 73573.0 28 101934.0 29 132400.0 30 157647.0 31 170930.0 32 178379.0 33 185642.0 34 188470.0 35 180099.0 36 149171.0 37 90322.0 38 27716.0 39 1179.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.409136562912046 20.08296878793216 15.448236139737908 23.059658509417886 2 15.714418296951857 21.044017105203665 39.58522022830735 23.656344369537127 3 19.16699426691631 24.995454843994064 30.240850434383653 25.596700454705974 4 13.344695002943533 17.636779066607588 36.73956568978834 32.27896024066053 5 13.492393430163704 37.697604860280734 34.254207578140736 14.555794131414821 6 31.552601709011213 36.964422964808456 17.231506533124882 14.251468793055446 7 28.07282636681504 32.90378861393183 20.74369282182659 18.279692197426538 8 26.06879930782838 35.81145747718104 19.4864585360932 18.63328467889738 9 26.082313249892803 16.869461840447702 20.143231279777385 36.904993629882114 10-11 24.8990941916042 27.027384606159195 27.183175274138748 20.890345928097858 12-13 25.775538652123984 24.143817606653666 27.855142995702014 22.225500745520335 14-15 22.47922985437459 25.64335457661836 26.148515167191523 25.728900401815523 16-17 21.981999003434677 28.846735011186375 26.794400578331512 22.37686540704744 18-19 21.946058674832198 27.18220932371434 29.324804200285502 21.546927801167957 20-21 22.590122771708014 26.448743751835245 29.436655043243988 21.524478433212753 22-23 22.259338278721334 26.38920682632969 29.35527363427956 21.996181260669413 24-25 22.14397656215422 26.570292359142343 29.421116429193177 21.864614649510262 26-27 21.8512384501348 26.84049466618123 29.284532996385426 22.023733887298537 28-29 21.79504416448 27.053400244957654 29.304968947203225 21.846586643359124 30-31 21.99973013473987 26.729344896131625 29.231476569912594 22.039448399215917 32-33 21.739286659318527 26.799747003158174 29.198434046635942 22.262532290887354 34-35 22.301922710831906 26.867528079192844 28.959356558157246 21.87119265181801 36-37 22.278977949443952 26.566892843304053 29.037476710343796 22.116652496908202 38-39 21.864743909686783 26.813586778142017 29.13882222566546 22.182847086505735 40-41 22.221992139255097 26.63301584264039 29.175273951458365 21.96971806664615 42-43 22.083568970868537 26.642131800880094 28.927054235668002 22.347244992583366 44-45 21.99739464261785 26.858221287812018 28.93239138056569 22.211992689004447 46-47 22.244855649223243 26.993241787759338 28.442831065640085 22.319071497377333 48-49 22.126101088806884 27.102470285359566 28.337708173858733 22.43372045197482 50-51 22.083299614895775 27.19652216134391 28.348645470860127 22.371532752900183 52-53 22.360307874729955 27.139201714403082 28.195352271134254 22.305138139732705 54-55 21.982534168657082 27.133178986930375 28.311934588095973 22.57235225631657 56-57 22.065923276192837 27.214978706367116 28.364548901477328 22.35454911596272 58-59 20.77481803032712 26.77041090266502 29.92479969727076 22.5299713697371 60-61 22.138551988026727 26.885229857757686 28.660358577624585 22.315859576591006 62-63 21.868239027533605 27.078893439697243 28.468304513486014 22.584563019283138 64-65 21.17693178433125 27.011331546023236 28.882478403336314 22.929258266309205 66-67 21.69186590947397 26.63263034311763 29.284406540808096 22.391097206600307 68-69 22.536011838100027 25.765835233524637 29.769761683575613 21.92839124479972 70-71 21.528647039091076 26.22200085980797 29.876428164894225 22.37292393620673 72-73 21.403119017994747 26.3405331882349 29.811785237172145 22.444562556598218 74-75 21.89034562825442 26.54556252824738 29.051983404504444 22.512108438993756 76-77 20.229054763970815 26.950064569084102 29.719831554724855 23.101049112220227 78-79 21.12689318351172 27.22288792965413 28.511975803194474 23.138243083639672 80-81 21.635926096991096 27.4847605919151 27.623513745747395 23.255799565346404 82-83 21.81266768591944 27.405462322349592 27.501573496255137 23.280296495475827 84-85 21.788692833102356 27.306616740021017 27.463692452370438 23.440997974506192 86-87 21.797407628625074 27.297546837111792 27.45786266190423 23.447182872358905 88-89 21.69269047413951 27.30365307343316 27.36328518187317 23.64037127055416 90-91 21.442430408923226 27.410218083960785 27.61399875928479 23.533352747831195 92-93 21.326099341837086 27.188063914031197 27.804892002641235 23.680944741490485 94-95 20.197811981674395 26.915191259887827 29.057776590239236 23.829220168198542 96-97 20.044129918054427 26.869054160168165 29.192287968931613 23.89452795284579 98-99 20.322034578819327 26.92385644257888 28.483877474625345 24.270231503976447 100-101 20.05636609527997 27.092737638406362 28.533860277075558 24.317035989238114 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 529.0 1 403.0 2 282.0 3 313.5 4 350.5 5 395.0 6 520.0 7 810.0 8 1188.0 9 1485.5 10 1671.5 11 1850.5 12 2077.5 13 2287.0 14 2448.0 15 2640.5 16 2871.5 17 3083.5 18 3284.0 19 3625.0 20 4112.0 21 4692.5 22 5545.5 23 6629.0 24 7815.5 25 9500.5 26 11990.0 27 14803.0 28 17555.0 29 20728.0 30 24439.0 31 28822.5 32 33646.5 33 37968.0 34 42359.0 35 46970.5 36 51213.0 37 55089.5 38 58769.0 39 62041.0 40 65356.0 41 69382.5 42 73214.0 43 76330.0 44 79321.0 45 81876.5 46 83230.0 47 84085.0 48 84463.0 49 83754.0 50 81597.5 51 78671.0 52 75767.5 53 72577.0 54 68344.0 55 62729.0 56 56182.0 57 49209.5 58 42487.0 59 35690.5 60 28857.5 61 23103.5 62 18154.0 63 13661.5 64 10146.5 65 7506.0 66 5475.0 67 3942.5 68 2850.0 69 1968.5 70 1381.5 71 1042.5 72 750.0 73 564.0 74 415.0 75 247.5 76 142.0 77 88.0 78 54.0 79 30.5 80 14.0 81 7.0 82 2.0 83 3.0 84 2.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020321556507490658 2 0.0048543296575270425 3 0.003759725911221925 4 0.0 5 0.05977488284170555 6 9.518293446131456E-5 7 0.0 8 1.9036586892262912E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9036586892262912E-4 16-17 0.0 18-19 0.001261173881612418 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.518293446131456E-5 28-29 0.001784680021149648 30-31 0.008019162228365753 32-33 2.379573361532864E-4 34-35 8.090549429211738E-4 36-37 0.023748142148097985 38-39 0.023010474406022795 40-41 0.025009316029710398 42-43 0.025366252033940332 44-45 0.025961145374323546 46-47 0.02795998699801115 48-49 0.027079544854243996 50-51 0.023177044541330096 52-53 0.020345352241105986 54-55 0.014991312177657043 56-57 0.1509601340556449 58-59 0.7702916928618033 60-61 0.3118192932952665 62-63 1.4277440169197184E-4 64-65 0.1471528166771923 66-67 1.4556326167168836 68-69 2.7916916720167406 70-71 2.2496486559931697 72-73 1.907822942608974 74-75 1.596574746920475 76-77 1.1414575457936995 78-79 0.43022686376514185 80-81 1.665701353073005E-4 82-83 2.1416160253795774E-4 84-85 4.759146723065728E-5 86-87 0.0024747562959941787 88-89 0.0 90-91 0.23060445446614983 92-93 0.17665952636019983 94-95 1.3246608988981148 96-97 1.3011745098197853 98-99 1.0248108596113585 100-101 0.41152341714349355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2101217.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.75061607837632 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41410254943916 45.481262179341535 2 13.170748566995371 15.475791850487402 3 4.212446429317042 7.424514687585983 4 1.7434525947683532 4.097156561843382 5 0.9297206776552713 2.731083129652635 6 0.5572065532268212 1.96417369709906 7 0.37195475038285886 1.5296799516790147 8 0.2603429575622819 1.2236247318760516 9 0.19757400867727004 1.0446835257777676 >10 1.0430245506126625 11.165098277303544 >50 0.06809277386035167 2.722690516592453 >100 0.02816991277574603 2.885664000381178 >500 0.002433595943715675 0.9400623409377927 >1k 5.678390535336298E-4 0.5931566993820697 >5k 1.6223972958103708E-4 0.7213578500600198 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8086 0.3848246040270948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7050 0.3355198439761338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4756 0.226345018149006 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.759146723065728E-5 5 0.0 0.0 0.0 0.0 4.759146723065728E-5 6 0.0 0.0 0.0 0.0 9.518293446131456E-5 7 0.0 0.0 0.0 0.0 9.518293446131456E-5 8 0.0 0.0 0.0 0.0 9.518293446131456E-5 9 0.0 0.0 0.0 0.0 9.518293446131456E-5 10-11 0.0 0.0 0.0 0.0 9.518293446131456E-5 12-13 0.0 0.0 0.0 0.0 9.518293446131456E-5 14-15 0.0 0.0 0.0 4.759146723065728E-5 9.518293446131456E-5 16-17 0.0 0.0 0.0 1.4277440169197184E-4 9.518293446131456E-5 18-19 0.0 0.0 0.0 2.1416160253795777E-4 1.189786680766432E-4 20-21 0.0 0.0 0.0 4.5211893869124414E-4 1.6657013530730047E-4 22-23 0.0 0.0 0.0 8.804421437671597E-4 1.9036586892262912E-4 24-25 0.0 0.0 0.0 0.001261173881612418 1.9036586892262912E-4 26-27 4.759146723065728E-5 0.0 0.0 0.0022130032262255637 1.9036586892262912E-4 28-29 4.759146723065728E-5 0.0 0.0 0.005663384600448216 1.9036586892262912E-4 30-31 4.759146723065728E-5 0.0 0.0 0.0201073949049527 1.9036586892262912E-4 32-33 4.759146723065728E-5 0.0 0.0 0.05285032435964491 1.9036586892262912E-4 34-35 4.759146723065728E-5 0.0 0.0 0.10125084653322336 1.9036586892262912E-4 36-37 4.759146723065728E-5 0.0 0.0 0.18784352115940428 1.9036586892262912E-4 38-39 4.759146723065728E-5 0.0 0.0 0.317720635231868 2.1416160253795777E-4 40-41 4.759146723065728E-5 0.0 0.0 0.4608757686616851 2.379573361532864E-4 42-43 4.759146723065728E-5 0.0 0.0 0.606696024256419 2.379573361532864E-4 44-45 4.759146723065728E-5 0.0 0.0 0.7843311756948473 2.379573361532864E-4 46-47 4.759146723065728E-5 0.0 0.0 0.9830731428500722 2.379573361532864E-4 48-49 4.759146723065728E-5 4.759146723065728E-5 0.0 1.1857889975190568 2.855488033839437E-4 50-51 4.759146723065728E-5 4.759146723065728E-5 0.0 1.3923597610337248 2.855488033839437E-4 52-53 4.759146723065728E-5 4.759146723065728E-5 0.0 1.584248556907735 2.855488033839437E-4 54-55 4.759146723065728E-5 4.759146723065728E-5 0.0 1.7883921555936393 3.33140270614601E-4 56-57 4.759146723065728E-5 4.759146723065728E-5 0.0 1.9700011945458273 3.33140270614601E-4 58-59 4.759146723065728E-5 4.759146723065728E-5 0.0 2.099378598212369 3.5693600422992964E-4 60-61 4.759146723065728E-5 9.518293446131456E-5 0.0 2.1983688500521366 3.8073173784525824E-4 62-63 1.189786680766432E-4 9.518293446131456E-5 0.0 2.2811304115662496 3.8073173784525824E-4 64-65 1.4277440169197184E-4 9.518293446131456E-5 0.0 2.3648200066913603 3.8073173784525824E-4 66-67 1.4277440169197184E-4 9.518293446131456E-5 0.0 2.4373731984844973 3.8073173784525824E-4 68-69 1.4277440169197184E-4 9.518293446131456E-5 0.0 2.522871269364373 3.8073173784525824E-4 70-71 1.6657013530730047E-4 9.518293446131456E-5 0.0 2.677281784794241 3.8073173784525824E-4 72-73 1.9036586892262912E-4 9.518293446131456E-5 0.0 2.870503141750709 3.8073173784525824E-4 74-75 1.9036586892262912E-4 9.518293446131456E-5 0.0 3.0929932510540317 3.8073173784525824E-4 76-77 1.9036586892262912E-4 9.518293446131456E-5 0.0 3.4182809295755745 3.8073173784525824E-4 78-79 1.9036586892262912E-4 9.518293446131456E-5 0.0 3.8751352192562694 3.8073173784525824E-4 80-81 1.9036586892262912E-4 9.518293446131456E-5 0.0 4.3807469671147725 3.8073173784525824E-4 82-83 1.9036586892262912E-4 9.518293446131456E-5 0.0 4.86641789020363 3.8073173784525824E-4 84-85 1.9036586892262912E-4 9.518293446131456E-5 0.0 5.256453759892482 4.2832320507591554E-4 86-87 1.9036586892262912E-4 9.518293446131456E-5 0.0 5.600468680769287 4.2832320507591554E-4 88-89 1.9036586892262912E-4 9.518293446131456E-5 0.0 5.96340120987028 4.2832320507591554E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4420 0.0 51.39519 1 GTATCAA 7725 0.0 40.083405 1 ATCAACG 9880 0.0 31.236925 3 TATCAAC 10055 0.0 30.74086 2 TCAACGC 10060 0.0 30.63118 4 CAACGCA 10270 0.0 30.004833 5 AACGCAG 10650 0.0 28.874872 6 ACGCAGA 12175 0.0 25.180742 7 CGCAGAG 12170 0.0 24.997608 8 GTGGTAT 2210 0.0 24.311419 1 TGGTATC 2260 0.0 23.142424 2 GTACATG 8740 0.0 22.378674 1 GCAGAGT 13375 0.0 21.935665 9 TACATGG 8725 0.0 21.817762 2 ACATGGG 8535 0.0 21.364433 3 GAGTACT 7450 0.0 20.98667 12-13 GTACTTT 7855 0.0 19.964798 14-15 AGTACTT 7815 0.0 19.735312 12-13 CATGGGG 4025 0.0 19.666512 4 AGAGTAC 12525 0.0 19.626791 10-11 >>END_MODULE