FastQCFastQC Report
Fri 27 May 2016
SRR939856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291586
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81320.35486340028259905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60930.2658857228138067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.1828427997029132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44200.052.3589171
GTATCAA82000.043.2519151
ATCAACG104000.033.3831863
TATCAAC105850.033.1086652
TCAACGC106400.032.542464
CAACGCA108800.031.7381385
AACGCAG112650.030.8615676
GTGGTAT21400.029.4536341
TGGTATC20500.028.6835212
ACGCAGA128150.026.945257
CGCAGAG129700.026.4781518
GCAGAGT134600.024.3259019
GTACATG91400.023.8277281
TACATGG90450.023.61152
ACATGGG95850.021.1538733
GAGTACT78550.020.57241812-13
CAGAGTA131000.020.46865810-11
AGAGTAC126300.020.1688410-11
GTACTTT81250.019.6914-15
TACCGTA1200.00503826819.601697
Produced by FastQC (version 0.11.5)