FastQCFastQC Report
Fri 27 May 2016
SRR939856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291586
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89310.3897300821352548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77270.33719005090797377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52350.22844440487941542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.055.0461921
GTATCAA85100.043.7209431
TATCAAC110250.033.3111462
ATCAACG110650.032.9662443
TCAACGC111050.032.8474964
CAACGCA113100.032.2101825
AACGCAG118650.030.7834646
ACGCAGA134150.026.8023627
CGCAGAG135900.026.2128948
GTGGTAT23100.024.4477021
TGGTATC22500.024.2517192
GCAGAGT146300.023.6686279
GTACATG90500.023.4926761
TACATGG92450.022.5825482
GAGTACT86100.021.62374912-13
ACATGGG94250.021.5908853
AGTACTT90000.020.66036812-13
AGAGTAC136900.020.52954910-11
GTACTTT90550.020.32533814-15
CAGAGTA142350.019.96015410-11
Produced by FastQC (version 0.11.5)