##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2291586 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.375094803337078 31.0 31.0 34.0 30.0 34.0 2 31.603820672669496 31.0 31.0 34.0 30.0 34.0 3 31.64197372474784 31.0 31.0 34.0 30.0 34.0 4 35.46157988397555 37.0 35.0 37.0 33.0 37.0 5 35.19165678268239 37.0 35.0 37.0 32.0 37.0 6 35.22008425605672 37.0 35.0 37.0 32.0 37.0 7 35.127495542388544 37.0 35.0 37.0 32.0 37.0 8 35.08742285910282 37.0 35.0 37.0 32.0 37.0 9 36.66681983569458 39.0 35.0 39.0 32.0 39.0 10-11 36.53007960425661 38.0 35.0 39.0 32.0 39.0 12-13 36.44590405945926 38.0 35.0 39.0 32.0 39.0 14-15 37.461948842417435 39.0 36.0 41.0 32.0 41.0 16-17 37.38244778943491 39.0 36.0 41.0 32.0 41.0 18-19 37.370867381804565 39.0 36.0 41.0 32.0 41.0 20-21 37.29154066223131 39.0 36.0 40.0 31.0 41.0 22-23 37.16534291097956 39.0 36.0 40.0 31.0 41.0 24-25 37.011330362465124 39.0 36.0 40.0 31.0 41.0 26-27 36.719116585631085 39.0 35.5 40.0 30.0 41.0 28-29 36.48504114617562 38.0 35.0 40.0 30.0 41.0 30-31 36.20762410836862 38.0 35.0 40.0 30.0 41.0 32-33 36.17826016566693 38.0 35.0 40.0 30.0 41.0 34-35 36.03041845254771 38.0 35.0 40.0 30.0 41.0 36-37 36.06607956236423 38.0 35.0 40.0 30.0 41.0 38-39 35.969564092292416 38.0 35.0 40.0 29.0 41.0 40-41 35.87673340647045 38.0 35.0 40.0 29.0 41.0 42-43 35.64390535637764 38.0 34.0 40.0 28.0 41.0 44-45 35.38172776409002 38.0 34.0 40.0 27.0 41.0 46-47 35.086094521436245 38.0 34.0 40.0 26.5 41.0 48-49 34.77477978133921 38.0 33.0 40.0 25.5 41.0 50-51 34.54456258678488 38.0 33.0 40.0 24.5 41.0 52-53 34.286376989560935 38.0 33.0 40.0 24.0 41.0 54-55 34.01891615675781 37.0 33.0 40.0 24.0 41.0 56-57 33.65304640541529 37.0 32.5 40.0 23.0 41.0 58-59 33.28484224462883 36.5 32.0 39.0 22.0 41.0 60-61 32.973269604544626 36.0 31.0 39.0 22.0 40.5 62-63 32.6798416031517 36.0 31.0 39.0 21.0 40.0 64-65 32.235405086259036 35.0 31.0 39.0 19.5 40.0 66-67 31.318436008947515 35.0 29.5 38.0 13.0 40.0 68-69 29.405669261376183 34.0 26.0 37.0 7.5 39.5 70-71 28.750732025767306 33.5 25.0 36.5 7.5 39.0 72-73 29.307506896970047 33.5 26.5 36.0 7.5 39.0 74-75 29.41273641923105 34.0 27.5 36.0 8.0 39.0 76-77 28.977768017434215 32.5 27.5 35.0 7.5 37.5 78-79 29.508466843487437 34.0 29.0 35.0 7.0 37.0 80-81 29.318705254788604 34.0 29.0 35.0 6.5 37.0 82-83 28.862433703120892 33.5 28.5 35.0 2.0 36.5 84-85 28.495303907424812 33.0 27.5 35.0 2.0 36.0 86-87 28.092728573136682 33.0 27.0 35.0 2.0 36.0 88-89 27.62258104212541 33.0 26.0 35.0 2.0 35.0 90-91 27.112347081890007 32.5 25.5 35.0 2.0 35.0 92-93 26.60663531719953 32.0 25.0 34.0 2.0 35.0 94-95 25.776691994103647 32.0 23.0 34.0 2.0 35.0 96-97 25.220785517104748 32.0 20.0 34.0 2.0 35.0 98-99 24.523587375730173 31.0 17.0 34.0 2.0 35.0 100-101 23.060264157661983 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18591566303406282 1101 2 0.15806815221537818 1101 3 0.1507946515931664 1101 4 0.1595038986419297 1101 5 0.09485752688076587 1101 6 0.17016364609496293 1101 7 0.14242154882074232 1101 8 0.21099343889481048 1101 9 0.2616283919681237 1101 10-11 0.2842244289216609 1101 12-13 0.3309112420277103 1101 14-15 0.29343392043099215 1101 16-17 0.4026677057811625 1101 18-19 0.22210076640479315 1101 20-21 0.3754159394368415 1101 22-23 0.2588862076515994 1101 24-25 0.18938011209912275 1101 26-27 0.25804802406572236 1101 28-29 0.1963801960504128 1101 30-31 0.03202917763660196 1101 32-33 -7.587214640523143E-4 1101 34-35 0.0233550173452457 1101 36-37 0.21149206264582432 1101 38-39 0.20728906111933298 1101 40-41 0.3659815131889488 1101 42-43 -0.029723429604828766 1101 44-45 -0.01168662848242974 1101 46-47 -0.020194277297498786 1101 48-49 0.03804333077950872 1101 50-51 0.021772087062842616 1101 52-53 -0.07776419989255601 1101 54-55 -0.20529350287527848 1101 56-57 -0.12480919753762265 1101 58-59 -0.1726983590508553 1101 60-61 -0.27183376287931793 1101 62-63 -0.27260503578416007 1101 64-65 -0.4257885200236089 1101 66-67 -0.5761260224359326 1101 68-69 -9.284555255424397 1101 70-71 -7.320919038667075 1101 72-73 -2.1098449642112698 1101 74-75 -0.9663141048888733 1101 76-77 -0.8190440147043319 1101 78-79 -1.0387528824897139 1101 80-81 -0.8817436619771719 1101 82-83 -0.7445637962509863 1101 84-85 -0.6781237657051555 1101 86-87 -0.9463927798606342 1101 88-89 -0.763136217171521 1101 90-91 -0.9543626225238135 1101 92-93 -1.2754647202593041 1101 94-95 -0.9171414438477932 1101 96-97 -0.9915694099841481 1101 98-99 -0.8565694137380433 1101 100-101 -1.0980772337518623 1102 1 0.12539637718897367 1102 2 0.07247698456794538 1102 3 0.19105355823461068 1102 4 0.15138101898990897 1102 5 0.1966884125514099 1102 6 0.17611864988020898 1102 7 0.22549029293285372 1102 8 0.17362566270916346 1102 9 0.18697534083811718 1102 10-11 0.26125066507155026 1102 12-13 0.16723708606072307 1102 14-15 0.3041939135590326 1102 16-17 0.31627956103962873 1102 18-19 0.17734110554719962 1102 20-21 0.22557461745356022 1102 22-23 0.23864281955027877 1102 24-25 0.2881108767553684 1102 26-27 0.1428633591019377 1102 28-29 0.0798571041984033 1102 30-31 -0.013589949813649582 1102 32-33 -0.039147285319444336 1102 34-35 -0.15595661646252523 1102 36-37 -0.10666359095468891 1102 38-39 0.10340347920239168 1102 40-41 0.05297973289901137 1102 42-43 0.14054327406468303 1102 44-45 0.09606763864413637 1102 46-47 0.07011598023097321 1102 48-49 0.2554933816584253 1102 50-51 0.01761606343388422 1102 52-53 0.03295595854003608 1102 54-55 -0.19676500785272566 1102 56-57 -0.2883464807358749 1102 58-59 -0.35450334272996287 1102 60-61 -0.1952942274160634 1102 62-63 -0.17539525797113953 1102 64-65 -0.11561481432082843 1102 66-67 0.26968349975539496 1102 68-69 -4.557543650501511 1102 70-71 0.30935438592735665 1102 72-73 0.5411256053115991 1102 74-75 0.17627664175680025 1102 76-77 0.13337089291126603 1102 78-79 0.0014281482660720712 1102 80-81 0.10220843066586838 1102 82-83 0.06849716971205666 1102 84-85 -0.18042275624488724 1102 86-87 -0.5242691374969048 1102 88-89 -0.5433478130324865 1102 90-91 -0.757849479906163 1102 92-93 -0.7024471571231992 1102 94-95 -0.44913927653829333 1102 96-97 -0.9998347317106706 1102 98-99 -0.6909619535113798 1102 100-101 -0.536887365734966 1103 1 0.18201696530832123 1103 2 0.21156513272283206 1103 3 0.16477142530884237 1103 4 0.21158220893541824 1103 5 0.3681941606189625 1103 6 0.19954153237460304 1103 7 0.19748798521488453 1103 8 0.207830151993889 1103 9 0.2979042503883136 1103 10-11 0.31115677188159196 1103 12-13 0.30909635317073025 1103 14-15 0.3163954795321757 1103 16-17 0.1740291038931261 1103 18-19 0.19741688724495532 1103 20-21 0.3244566175566632 1103 22-23 0.2307208422514293 1103 24-25 0.1699021177375286 1103 26-27 0.20088343715799795 1103 28-29 0.19467346473611968 1103 30-31 0.26641227905980713 1103 32-33 0.20488138072252582 1103 34-35 0.014238341734731819 1103 36-37 0.21014840735925588 1103 38-39 0.2554746257531022 1103 40-41 0.1905520196625261 1103 42-43 0.11596198386534695 1103 44-45 0.1735780400536342 1103 46-47 0.16128884528860965 1103 48-49 0.17494614015468102 1103 50-51 -0.08207030222872902 1103 52-53 0.1371072732721288 1103 54-55 0.2688781206971669 1103 56-57 0.3260819117763276 1103 58-59 0.18985199731729097 1103 60-61 -0.06243289973527055 1103 62-63 0.0031846994528308414 1103 64-65 -0.10155926533564497 1103 66-67 0.1506368283580528 1103 68-69 -4.2870095851272865 1103 70-71 0.40629692496296954 1103 72-73 0.5353375469181465 1103 74-75 0.3780152767764946 1103 76-77 0.1606937353769169 1103 78-79 0.03149500688877538 1103 80-81 0.04291395251034302 1103 82-83 0.03425818595706076 1103 84-85 -0.07104609785689675 1103 86-87 -0.13635910817281527 1103 88-89 -0.22010089024248458 1103 90-91 -0.10553952548434964 1103 92-93 -0.16507713683590453 1103 94-95 -0.040323323869255745 1103 96-97 -0.6902032691148072 1103 98-99 -0.2919481163010218 1103 100-101 -0.3722479125519236 1104 1 0.19711525765210425 1104 2 0.22746741397223502 1104 3 0.26433868685111506 1104 4 0.22005948122703245 1104 5 0.1813439687085463 1104 6 0.20730126023486406 1104 7 0.19884289636692642 1104 8 0.18998664856339076 1104 9 0.13787875571948405 1104 10-11 0.22528211231431072 1104 12-13 0.26719962869748315 1104 14-15 0.3544559309455906 1104 16-17 0.3373244667829809 1104 18-19 0.24185450788016283 1104 20-21 0.27971137255570255 1104 22-23 0.26430424402765595 1104 24-25 0.1790281843775574 1104 26-27 0.3693939454146431 1104 28-29 0.22676915526711383 1104 30-31 0.09431681339503939 1104 32-33 0.12847054792125334 1104 34-35 0.13310483902674974 1104 36-37 0.27748312331267044 1104 38-39 0.4554775563866613 1104 40-41 0.26155187073474195 1104 42-43 0.17429523118428847 1104 44-45 0.24975949087575344 1104 46-47 0.12499571336659443 1104 48-49 0.2127236656235425 1104 50-51 -0.009004078020147688 1104 52-53 -0.14669749271217114 1104 54-55 -0.08971475495106773 1104 56-57 0.09225163637786693 1104 58-59 0.1424092792228535 1104 60-61 0.16638064709072609 1104 62-63 0.11379796125167019 1104 64-65 0.2627918444713231 1104 66-67 0.3786604864965142 1104 68-69 -3.954709100448415 1104 70-71 0.6681769577270806 1104 72-73 0.9526384776431307 1104 74-75 0.6952199573853548 1104 76-77 0.539811644165276 1104 78-79 0.26588477525444887 1104 80-81 -0.008433143618862715 1104 82-83 -0.03596200599759314 1104 84-85 0.04897366825274574 1104 86-87 0.08064140496453476 1104 88-89 0.06702655066193586 1104 90-91 0.035477308517190664 1104 92-93 -0.2469037435972261 1104 94-95 0.181480110612096 1104 96-97 -0.28546729155129213 1104 98-99 -0.009905048152550222 1104 100-101 0.03996578851018029 1105 1 0.02631313844387151 1105 2 0.12171636283372322 1105 3 0.141987361916879 1105 4 0.10695488550302912 1105 5 0.21028650468200283 1105 6 0.1195604039375695 1105 7 0.022963380789050802 1105 8 0.18148117381100803 1105 9 0.2358132017211716 1105 10-11 0.1251448211184183 1105 12-13 0.027099246466917748 1105 14-15 0.16318823385297776 1105 16-17 0.10100419622688861 1105 18-19 0.023879838186260827 1105 20-21 0.126022022537164 1105 22-23 0.057196121534367705 1105 24-25 0.0025393597807763513 1105 26-27 0.07711075116932165 1105 28-29 0.07411838391372783 1105 30-31 0.06486946990548148 1105 32-33 0.23461752477055597 1105 34-35 0.1644983777408484 1105 36-37 0.14109221367402114 1105 38-39 0.32336137503069295 1105 40-41 0.1888580687197603 1105 42-43 0.13574105998310415 1105 44-45 -0.006489993991728227 1105 46-47 0.20362464649833356 1105 48-49 0.09888505634992129 1105 50-51 0.3280750397189891 1105 52-53 0.2683662067196977 1105 54-55 0.2384806623228073 1105 56-57 0.22153867867424992 1105 58-59 0.2485912090983291 1105 60-61 0.22487413411725043 1105 62-63 0.17772025218206267 1105 64-65 0.40091744329890133 1105 66-67 0.9140328595888718 1105 68-69 2.1991972086353826 1105 70-71 2.269319573046591 1105 72-73 1.386492602127035 1105 74-75 0.8716206395683059 1105 76-77 0.6029327867055088 1105 78-79 0.3439900387511017 1105 80-81 0.3218257537141831 1105 82-83 0.42016104009695354 1105 84-85 0.29245689553805576 1105 86-87 0.1895545875475193 1105 88-89 0.2646220864006459 1105 90-91 0.22809217211725397 1105 92-93 0.2974877617625644 1105 94-95 0.5841817024140319 1105 96-97 0.11876374097897724 1105 98-99 0.40306674395078446 1105 100-101 0.6067880184917946 1106 1 0.3897193164900976 1106 2 0.28432506875856234 1106 3 0.3003589346459883 1106 4 0.2701087032264198 1106 5 0.3655685480017965 1106 6 0.3317005893655889 1106 7 0.2658368456580291 1106 8 0.3431791098680961 1106 9 0.3428064404221942 1106 10-11 0.3172424439471726 1106 12-13 0.3524122919654644 1106 14-15 0.5743150401335271 1106 16-17 0.4251749821723436 1106 18-19 0.4683375025762757 1106 20-21 0.45803983064768516 1106 22-23 0.515274267726717 1106 24-25 0.5594537569765734 1106 26-27 0.5286011453318409 1106 28-29 0.5291084070739345 1106 30-31 0.545515750853518 1106 32-33 0.6111061047065007 1106 34-35 0.6264541871193572 1106 36-37 0.6184841378061563 1106 38-39 0.6693135683321785 1106 40-41 0.6266523179871797 1106 42-43 0.4524076985222365 1106 44-45 0.5881614410474256 1106 46-47 0.6404622780016282 1106 48-49 0.6451647105161484 1106 50-51 0.5993747628876989 1106 52-53 0.6706421187974954 1106 54-55 0.742815116820033 1106 56-57 0.6130608682122585 1106 58-59 0.5438086265735222 1106 60-61 0.6441936500759695 1106 62-63 0.7364736281905095 1106 64-65 0.5912691894849473 1106 66-67 1.2052356327396723 1106 68-69 2.653304026920761 1106 70-71 2.9794837985237344 1106 72-73 1.7515068318514366 1106 74-75 1.3841407718577727 1106 76-77 1.0491489851329519 1106 78-79 0.9054467529926953 1106 80-81 0.9257092111743646 1106 82-83 1.0014594862751771 1106 84-85 0.9714406136280616 1106 86-87 0.8464286498455316 1106 88-89 1.0123273007409672 1106 90-91 1.0425642996902909 1106 92-93 0.9125263218123401 1106 94-95 1.0395123501294812 1106 96-97 0.8507251252366679 1106 98-99 1.0947925206507172 1106 100-101 1.1904868692566133 1107 1 0.060782111701787045 1107 2 0.0346773590476559 1107 3 0.0175263787645612 1107 4 0.026555686495349562 1107 5 -0.01749672294232596 1107 6 0.033557861876914785 1107 7 0.18642526744485366 1107 8 0.028833584309666094 1107 9 -0.1080268952770922 1107 10-11 0.02255817081029221 1107 12-13 0.02527179131104873 1107 14-15 0.019077626289671912 1107 16-17 -0.03456663691502371 1107 18-19 0.01605800222753828 1107 20-21 -0.04320030688038656 1107 22-23 -0.08483768913345813 1107 24-25 -0.04554720360003017 1107 26-27 -0.011414394242493131 1107 28-29 -0.03540155216543184 1107 30-31 -0.06350477330900617 1107 32-33 -0.08128404649677634 1107 34-35 0.08197977394649314 1107 36-37 -0.11014255940968809 1107 38-39 0.05408544794874359 1107 40-41 -0.024568371413444368 1107 42-43 0.009331373849235547 1107 44-45 -0.0018646506739159463 1107 46-47 0.10793128639318894 1107 48-49 -0.10655055754151022 1107 50-51 0.05713740488032926 1107 52-53 -0.0914953581699578 1107 54-55 -0.02042839550232145 1107 56-57 0.06491648455701693 1107 58-59 0.16032108047294003 1107 60-61 0.12862227734101594 1107 62-63 0.18027204148829412 1107 64-65 0.02814586753657977 1107 66-67 0.4686311338341582 1107 68-69 1.8093279779285147 1107 70-71 2.1057801274175922 1107 72-73 1.057458432039578 1107 74-75 0.4101743854144857 1107 76-77 0.0847647999148542 1107 78-79 0.2379793534486616 1107 80-81 0.19529707175731303 1107 82-83 0.2764496675733703 1107 84-85 0.41093034397575323 1107 86-87 0.34488926667767217 1107 88-89 0.16918482404323143 1107 90-91 0.1604720975849716 1107 92-93 0.09293159996787992 1107 94-95 0.458718487977432 1107 96-97 0.24186028073178178 1107 98-99 0.3664194535154017 1107 100-101 0.3924148364209792 1108 1 -0.1318866557127194 1108 2 -0.18225944869617905 1108 3 -0.06857912406264433 1108 4 -0.049555539926267045 1108 5 0.08453295169334751 1108 6 0.05171486447454754 1108 7 0.11697731127122779 1108 8 -0.020645945978216673 1108 9 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-0.6501661821449147 2316 54-55 -0.7220374096530193 2316 56-57 -0.8204579164225265 2316 58-59 -0.700822007971432 2316 60-61 -0.842031250420213 2316 62-63 -0.7538475946340277 2316 64-65 -0.7046782047102695 2316 66-67 -0.13953325913244186 2316 68-69 1.1761723735018457 2316 70-71 1.3943733827753881 2316 72-73 0.5183607649724173 2316 74-75 0.024576760110100793 2316 76-77 -0.451827376035844 2316 78-79 -0.5656914567583442 2316 80-81 -0.7190349946188768 2316 82-83 -0.6502248131684851 2316 84-85 -0.6332252081327177 2316 86-87 -0.5840056168776186 2316 88-89 -0.606062700709245 2316 90-91 -0.7170813748291103 2316 92-93 -0.722042328128552 2316 94-95 -0.3475541889797036 2316 96-97 -0.2665902101873385 2316 98-99 -0.4701040662695988 2316 100-101 -0.7711328699766149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 48.0 5 100.0 6 218.0 7 575.0 8 1329.0 9 2355.0 10 3860.0 11 5798.0 12 8125.0 13 10530.0 14 12950.0 15 16140.0 16 19979.0 17 23309.0 18 21336.0 19 18342.0 20 18148.0 21 19061.0 22 20376.0 23 23385.0 24 27994.0 25 34215.0 26 42146.0 27 52329.0 28 63782.0 29 77550.0 30 91973.0 31 109064.0 32 129875.0 33 157825.0 34 194697.0 35 244203.0 36 303391.0 37 333058.0 38 188816.0 39 14683.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.569436909086704 20.08567247084417 15.362338071042503 22.982552549026625 2 15.760830898095385 21.066994323476713 39.49681691302763 23.675357865400272 3 19.2267881081383 24.93188117913199 30.246938362285654 25.59439235044406 4 13.390682261106502 17.635428039794274 36.75807933893819 32.21581036016104 5 13.550746077171008 37.5949669792013 34.286952355268355 14.56733458835933 6 31.647513992492538 36.903742648104846 17.215195065775408 14.233548293627207 7 27.998338268779786 32.91253306661849 20.755406953961142 18.333721710640578 8 26.050909719294847 35.82318097597035 19.499988217767083 18.625921086967715 9 26.053702544875033 16.85478965223212 20.10878055634831 36.982727246544535 10-11 24.84100531247791 27.072582045797105 27.191909882500592 20.894502759224398 12-13 25.817076033803666 24.183665810491075 27.78477438769481 22.214483768010453 14-15 22.476594812501034 25.635978750088366 26.146105797469527 25.741320639941073 16-17 21.98934053535587 28.83370567493106 26.8678991359013 22.30905465381177 18-19 21.884472151601557 27.230005768930337 29.34598570596958 21.53953637349853 20-21 22.59197341928254 26.394383627758245 29.48340145209475 21.530241500864467 22-23 22.26047811428417 26.415417095409033 29.363004486848848 21.961100303457954 24-25 22.132708670663757 26.59815799860664 29.439010186624227 21.830123144105375 26-27 21.837649934826597 26.859842056503396 29.240078565914594 22.06242944275541 28-29 21.823014920253815 27.047198377715315 29.27685796237797 21.852928739652892 30-31 21.958029721056356 26.69874505470912 29.248629980965045 22.094595243269474 32-33 21.748603880986117 26.805790539163553 29.200805051295397 22.244800528554926 34-35 22.284646728161157 26.880717650041742 28.957084049210142 21.877551572586963 36-37 22.284015668835856 26.544807650515505 29.053721566927265 22.11745511372137 38-39 21.87709795394755 26.829415842263128 29.107206893584568 22.186279310204753 40-41 22.228022369249885 26.64720292641929 29.171257034073705 21.953517670257128 42-43 22.069292543154674 26.662796282596574 28.911441375148332 22.35646979910042 44-45 22.00199402230116 26.85482840597246 28.949054149826857 22.19412342189952 46-47 22.18220611969015 27.024802412759147 28.455445414818552 22.33754605273215 48-49 22.058132383824336 27.14268492537991 28.36812459160747 22.43105809918828 50-51 22.093335604591434 27.225046735503263 28.298543774132117 22.38307388577319 52-53 22.36290006155171 27.13502445146596 28.14670835908905 22.355367127893274 54-55 22.009161350636603 27.145408113696632 28.29643896759381 22.54899156807295 56-57 22.048622592688385 27.318249132674833 28.176112571658685 22.457015702978097 58-59 22.097577698670804 27.022485113614863 28.122507524752088 22.75742966296225 60-61 21.9687115470683 27.08875724925388 28.376837039544046 22.565694164133777 62-63 21.89817286668073 27.11154352279613 28.401396353239228 22.58888725728391 64-65 21.68491883025848 27.021333745879 28.340185213510928 22.953562210351595 66-67 21.515194163427186 27.195555085453236 28.330560798070557 22.958689953049017 68-69 21.591371207911696 26.94729332494299 28.665356697756515 22.795978769388796 70-71 21.473349939943247 27.023369228642615 28.427709218859153 23.07557161255498 72-73 21.560092188040695 27.07469544085413 28.260098286983915 23.105114084121258 74-75 21.77894219690649 27.342239771931382 27.72558431997702 23.15323371118511 76-77 21.720470896781304 27.234106731627378 27.818853550507338 23.226568821083973 78-79 21.815294024975742 27.322058588321397 27.67635181620829 23.18629557049458 80-81 21.772236855656157 27.436968592519666 27.51885618330951 23.271938368514668 82-83 21.77306934434696 27.459896215960523 27.486886262807996 23.28014817688452 84-85 21.81164561999743 27.350349585045038 27.424425307670624 23.413579487286903 86-87 21.786288443311008 27.349888023683473 27.447136356257495 23.416687176748027 88-89 21.72691842611579 27.307611913008458 27.34119137939386 23.624278281481896 90-91 21.62436964668795 27.41700893535093 27.3964335399455 23.562187878015614 92-93 21.71044721993905 27.155231006795262 27.437946850643254 23.696374922622436 94-95 21.40096360958788 27.035201622553632 27.65778757614455 23.906047191713935 96-97 21.664301797722306 26.867260800889674 27.540691967490943 23.927745433897076 98-99 21.51662410454787 26.939049678307196 27.30089679424536 24.24342942289957 100-101 21.2960301972365 26.913081912273434 27.609007934455693 24.181879956034376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1008.0 1 677.0 2 431.0 3 549.0 4 488.0 5 464.5 6 781.0 7 1714.5 8 2714.5 9 3127.5 10 2847.0 11 2392.5 12 2364.5 13 2421.0 14 2554.0 15 2703.5 16 2867.5 17 3068.0 18 3388.0 19 3786.5 20 4174.5 21 4919.5 22 5811.0 23 6821.0 24 8211.0 25 10187.5 26 12785.0 27 16024.5 28 19060.5 29 22140.5 30 26244.5 31 30747.5 32 36356.5 33 41076.5 34 44642.5 35 48603.5 36 53312.0 37 58181.0 38 61823.0 39 64254.5 40 67115.0 41 71499.0 42 75055.0 43 78466.5 44 82438.5 45 85681.0 46 86887.5 47 88121.0 48 90457.5 49 89807.5 50 86929.0 51 84111.0 52 81263.0 53 79391.0 54 77508.5 55 71868.5 56 64160.5 57 57452.5 58 51586.0 59 44870.0 60 36894.0 61 29941.0 62 24282.5 63 18518.0 64 13532.0 65 10220.0 66 7650.5 67 5913.5 68 4297.0 69 2891.5 70 2028.0 71 1483.5 72 1177.5 73 939.5 74 674.0 75 473.0 76 333.0 77 188.0 78 105.0 79 59.5 80 37.0 81 25.5 82 16.0 83 8.0 84 3.5 85 2.0 86 2.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023346276334381518 2 0.024917240723237095 3 8.727579938086548E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.2728424767824554E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0909474922608185E-4 26-27 3.0546529783302917E-4 28-29 1.3091369907129822E-4 30-31 5.236547962851929E-4 32-33 0.002400084482973801 34-35 0.004669255266876303 36-37 0.10161084942917264 38-39 0.0939523980335017 40-41 0.10350909806570646 42-43 0.10505824350471682 44-45 0.09790162795548585 46-47 0.09124684825269486 48-49 0.09259962314309828 50-51 0.09190141674805136 52-53 0.07163161234184534 54-55 0.062031274409950134 56-57 0.033972104909001884 58-59 0.013724119452641095 60-61 0.010014897978954313 62-63 0.0019855244359146896 64-65 0.0053456427120780105 66-67 0.16006381606450729 68-69 0.4188365612287735 70-71 0.3997886180138996 72-73 0.1878393392174677 74-75 0.0303937971343864 76-77 0.03340481221302626 78-79 0.0053456427120780105 80-81 0.0013091369907129822 82-83 2.181894984521637E-5 84-85 5.236547962851929E-4 86-87 0.0013091369907129822 88-89 3.0546529783302917E-4 90-91 6.109305956660583E-4 92-93 0.0027273687306520462 94-95 0.08125376922358576 96-97 0.01784790097338699 98-99 0.018546107368433914 100-101 0.010931293872453401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2291586.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.97156219615134 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02641056362548 44.65341350735503 2 13.327149784600367 15.451913852707703 3 4.249291739832582 7.390142411558901 4 1.7964075228069964 4.165622017521598 5 0.9937950565359929 2.880592596510202 6 0.5604537392547065 1.949422728196188 7 0.3853080184205797 1.5635835428181124 8 0.2703828297886532 1.2539612027091447 9 0.21465093374998914 1.1199284960714508 >10 1.0698446723299024 11.302430165302011 >50 0.07327005774129335 2.9015512239650754 >100 0.030096997475640798 3.107265065541712 >500 0.0020340580415437117 0.7990440693990377 >1k 6.780193471810833E-4 0.5049927073928424 >5k 2.260064490603611E-4 0.956136412950921 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8931 0.3897300821352548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7727 0.33719005090797377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5235 0.22844440487941542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.727579938086548E-5 0.0 3 0.0 0.0 0.0 8.727579938086548E-5 0.0 4 0.0 0.0 0.0 8.727579938086548E-5 4.363789969043274E-5 5 0.0 0.0 0.0 1.3091369907129822E-4 4.363789969043274E-5 6 0.0 0.0 0.0 1.3091369907129822E-4 8.727579938086548E-5 7 0.0 0.0 0.0 1.3091369907129822E-4 8.727579938086548E-5 8 0.0 0.0 0.0 1.3091369907129822E-4 8.727579938086548E-5 9 0.0 0.0 0.0 1.7455159876173095E-4 8.727579938086548E-5 10-11 0.0 0.0 0.0 1.7455159876173095E-4 8.727579938086548E-5 12-13 0.0 0.0 0.0 1.7455159876173095E-4 8.727579938086548E-5 14-15 0.0 0.0 0.0 2.181894984521637E-4 1.0909474922608185E-4 16-17 0.0 0.0 0.0 2.836463479878128E-4 1.3091369907129822E-4 18-19 0.0 0.0 0.0 3.0546529783302917E-4 1.3091369907129822E-4 20-21 0.0 0.0 0.0 3.9274109721389465E-4 1.3091369907129822E-4 22-23 0.0 0.0 0.0 5.236547962851929E-4 1.5273264891651459E-4 24-25 0.0 0.0 0.0 0.0010691285424156022 2.181894984521637E-4 26-27 0.0 0.0 0.0 0.002007343385759906 2.4000844829738006E-4 28-29 0.0 0.0 0.0 0.006152943856351016 2.836463479878128E-4 30-31 0.0 0.0 0.0 0.02109892450032423 3.0546529783302917E-4 32-33 0.0 0.0 0.0 0.05498375360994526 3.0546529783302917E-4 34-35 0.0 0.0 0.0 0.10438185605951511 3.0546529783302917E-4 36-37 0.0 0.0 0.0 0.19122127644347625 3.0546529783302917E-4 38-39 0.0 0.0 0.0 0.3240332241513083 3.0546529783302917E-4 40-41 0.0 0.0 0.0 0.4667727940387138 3.0546529783302917E-4 42-43 0.0 0.0 0.0 0.6150761961366494 3.0546529783302917E-4 44-45 0.0 0.0 0.0 0.7901514496946656 3.0546529783302917E-4 46-47 0.0 0.0 0.0 0.9987842481146245 3.0546529783302917E-4 48-49 0.0 0.0 0.0 1.2206611490906298 3.0546529783302917E-4 50-51 0.0 0.0 0.0 1.4568512811650969 3.0546529783302917E-4 52-53 0.0 0.0 0.0 1.6837465406055019 3.0546529783302917E-4 54-55 0.0 0.0 0.0 1.9243877384483934 3.0546529783302917E-4 56-57 0.0 0.0 0.0 2.203517563818246 3.0546529783302917E-4 58-59 0.0 0.0 0.0 2.4863129727620956 3.0546529783302917E-4 60-61 0.0 0.0 0.0 2.78368344020255 3.0546529783302917E-4 62-63 0.0 0.0 0.0 3.0693153126262773 3.491031975234619E-4 64-65 0.0 0.0 0.0 3.358940052871679 3.491031975234619E-4 66-67 0.0 0.0 0.0 3.677147617414315 3.491031975234619E-4 68-69 0.0 0.0 0.0 4.0300473122108444 3.491031975234619E-4 70-71 0.0 0.0 0.0 4.436839813125058 3.491031975234619E-4 72-73 0.0 0.0 0.0 4.850396188491289 4.363789969043274E-4 74-75 0.0 0.0 0.0 5.261421565675476 4.363789969043274E-4 76-77 0.0 0.0 0.0 5.702797102094357 4.363789969043274E-4 78-79 0.0 0.0 0.0 6.198545461527519 4.363789969043274E-4 80-81 0.0 0.0 0.0 6.719931087028808 4.5819794674954376E-4 82-83 0.0 0.0 0.0 7.248080586982116 4.800168965947601E-4 84-85 0.0 0.0 0.0 7.758425823861727 4.800168965947601E-4 86-87 0.0 0.0 0.0 8.270058378782206 4.800168965947601E-4 88-89 0.0 0.0 0.0 8.830303553957826 4.800168965947601E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4440 0.0 55.046192 1 GTATCAA 8510 0.0 43.720943 1 TATCAAC 11025 0.0 33.311146 2 ATCAACG 11065 0.0 32.966244 3 TCAACGC 11105 0.0 32.847496 4 CAACGCA 11310 0.0 32.210182 5 AACGCAG 11865 0.0 30.783464 6 ACGCAGA 13415 0.0 26.802362 7 CGCAGAG 13590 0.0 26.212894 8 GTGGTAT 2310 0.0 24.447702 1 TGGTATC 2250 0.0 24.251719 2 GCAGAGT 14630 0.0 23.668627 9 GTACATG 9050 0.0 23.492676 1 TACATGG 9245 0.0 22.582548 2 GAGTACT 8610 0.0 21.623749 12-13 ACATGGG 9425 0.0 21.590885 3 AGTACTT 9000 0.0 20.660368 12-13 AGAGTAC 13690 0.0 20.529549 10-11 GTACTTT 9055 0.0 20.325338 14-15 CAGAGTA 14235 0.0 19.960154 10-11 >>END_MODULE