FastQCFastQC Report
Fri 27 May 2016
SRR939855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386723
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.22784378413414544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.16700723125389919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.1053746077781125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33150.051.9387051
GTATCAA63450.044.1798131
TATCAAC81450.034.8246882
TCAACGC82150.034.300594
ATCAACG82600.034.1704333
CAACGCA84650.033.17555
AACGCAG89300.031.6073596
ACGCAGA102250.027.4187017
CGCAGAG102250.027.2325538
GCAGAGT110850.023.5364289
TGGTATC16250.023.0595532
GTACATG75900.021.3098641
TACATGG76350.020.8119582
AGAGTAC100150.020.57752610-11
CAGAGTA106000.019.88933410-11
GAGTACT65350.019.88737912-13
ACATGGG78600.019.3729653
GTGGTAT19900.019.3063411
GTACTTT68700.018.919814-15
GTATAGG7550.018.8470041
Produced by FastQC (version 0.11.5)