FastQCFastQC Report
Fri 27 May 2016
SRR939855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386723
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.25930952188419015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52290.21908700758320088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.14061120624387496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.051.6835021
GTATCAA66650.039.953291
ATCAACG81600.032.2213
TCAACGC82500.031.868834
TATCAAC84100.031.3224932
CAACGCA84900.030.9679475
AACGCAG88700.029.6750836
ACGCAGA105000.024.8897
CGCAGAG105950.024.621398
TGGTATC19000.022.3218122
GTACATG79500.022.2314821
TACATGG77300.022.1292972
GTGGTAT21350.022.0752891
GCAGAGT119600.021.1026679
ACATGGG79350.020.6052343
TAAGGTG14550.020.4015645
GAGTACT69150.020.08827812-13
GTACTTT70450.019.48406414-15
AGGTAAG13750.019.1922452
AGAGTAC107600.019.12374510-11
Produced by FastQC (version 0.11.5)