##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939855_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2386723 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.426111869705867 31.0 31.0 34.0 30.0 34.0 2 31.636605504702473 31.0 31.0 34.0 30.0 34.0 3 31.698613119327213 31.0 31.0 34.0 30.0 34.0 4 35.504584738153525 37.0 35.0 37.0 33.0 37.0 5 35.228533851645125 37.0 35.0 37.0 32.0 37.0 6 35.253619712048696 37.0 35.0 37.0 32.0 37.0 7 35.175635379555985 37.0 35.0 37.0 32.0 37.0 8 35.11457131807922 37.0 35.0 37.0 32.0 37.0 9 36.73158594440997 39.0 35.0 39.0 32.0 39.0 10-11 36.585721300712315 38.5 35.0 39.0 32.0 39.0 12-13 36.52559848796865 38.0 35.0 39.0 32.0 39.0 14-15 37.57749014024669 39.0 36.0 41.0 32.0 41.0 16-17 37.50143627894816 39.0 36.0 41.0 32.0 41.0 18-19 37.467352935384625 39.0 36.0 41.0 32.0 41.0 20-21 37.38251673947919 39.0 36.0 41.0 32.0 41.0 22-23 37.240756258686076 39.0 36.0 40.0 31.0 41.0 24-25 37.08032666547396 39.0 36.0 40.0 31.0 41.0 26-27 36.786577453688594 39.0 36.0 40.0 30.5 41.0 28-29 36.56513407714259 38.0 35.0 40.0 30.0 41.0 30-31 36.312403450253754 38.0 35.0 40.0 30.0 41.0 32-33 36.28285561416218 38.0 35.0 40.0 30.0 41.0 34-35 36.37032910815373 38.0 35.0 40.0 30.0 41.0 36-37 36.34834918002633 38.0 35.0 40.0 30.0 41.0 38-39 36.21631123511191 38.0 35.0 40.0 30.0 41.0 40-41 36.04649534110159 38.0 35.0 40.0 29.5 41.0 42-43 35.854372082558385 38.0 35.0 40.0 29.0 41.0 44-45 35.61836145208305 38.0 34.0 40.0 28.0 41.0 46-47 35.33450404592406 38.0 34.0 40.0 27.0 41.0 48-49 35.033174147146525 38.0 34.0 40.0 26.0 41.0 50-51 34.764237408362845 38.0 33.0 40.0 26.0 41.0 52-53 34.47373511714598 38.0 33.0 40.0 24.5 41.0 54-55 34.16349676941983 37.5 33.0 40.0 24.0 41.0 56-57 33.0847421338798 36.5 32.0 39.5 18.0 41.0 58-59 30.025529146029932 35.0 26.0 39.0 8.0 40.0 60-61 30.002442889267 34.5 26.0 38.0 10.0 40.0 62-63 31.529714172947592 35.0 29.5 38.5 13.5 40.0 64-65 30.970647410696593 35.0 29.5 38.0 7.5 40.0 66-67 25.58203863623889 29.0 18.0 36.0 2.0 39.0 68-69 17.785142222201742 13.5 7.0 29.5 2.0 35.5 70-71 16.625409400252984 15.0 7.5 26.0 2.0 33.5 72-73 18.854806988494268 19.5 7.5 30.0 2.0 34.5 74-75 19.63311284971067 21.0 9.0 31.0 2.0 35.0 76-77 20.732847297319378 23.5 9.5 31.5 2.0 34.5 78-79 23.95450205155772 29.0 14.5 34.0 2.0 36.0 80-81 26.54817337412008 31.5 23.5 35.0 2.0 36.0 82-83 27.29537361478479 33.0 26.0 35.0 2.0 36.0 84-85 27.222076252669453 33.0 26.0 35.0 2.0 36.0 86-87 26.917416264895422 33.0 26.0 35.0 2.0 35.5 88-89 26.46954066307653 33.0 25.0 35.0 2.0 35.0 90-91 25.023987701966252 32.0 15.0 34.5 2.0 35.0 92-93 23.15324233268796 31.0 2.0 34.0 2.0 35.0 94-95 18.651053557534745 25.0 2.0 33.0 2.0 35.0 96-97 17.312635777172297 17.5 2.0 32.5 2.0 34.5 98-99 16.18351689743636 7.5 2.0 32.0 2.0 34.0 100-101 15.540467410755248 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34019150240667173 1101 2 0.3961039522601695 1101 3 0.4254199351293302 1101 4 0.30656370589181847 1101 5 0.43747621712425655 1101 6 0.4720844111982814 1101 7 0.40805120140991136 1101 8 0.428932998625811 1101 9 0.5750778236421112 1101 10-11 0.41190877696964634 1101 12-13 0.525870191688746 1101 14-15 0.5546421147225686 1101 16-17 0.3882718988910696 1101 18-19 0.36017488064313596 1101 20-21 0.7603066200228454 1101 22-23 0.6904576572562888 1101 24-25 0.5526996583222541 1101 26-27 0.7418494267493116 1101 28-29 0.5382210099310143 1101 30-31 0.730212707349466 1101 32-33 0.4693237245076176 1101 34-35 0.7945216692136512 1101 36-37 0.7239841024736506 1101 38-39 0.7056294887501977 1101 40-41 0.9071669747741353 1101 42-43 1.0097898415967208 1101 44-45 0.9095854000971286 1101 46-47 0.4764295186748413 1101 48-49 0.5195622396564801 1101 50-51 0.5378470464834564 1101 52-53 0.7794051612383086 1101 54-55 0.9937946083125482 1101 56-57 1.4267447865367089 1101 58-59 -7.868722290459278 1101 60-61 -9.132761488090974 1101 62-63 -1.9330935159919385 1101 64-65 0.5332556448010024 1101 66-67 -1.0948856106005778 1101 68-69 -3.444824730145191 1101 70-71 -4.0455276886131415 1101 72-73 -7.133237267554719 1101 74-75 -7.640424879636665 1101 76-77 -8.6639252754038 1101 78-79 -11.660167640036914 1101 80-81 -7.536345649256646 1101 82-83 -3.5986247739725137 1101 84-85 -2.636230160631875 1101 86-87 -2.4522603335721307 1101 88-89 -2.7517533207706855 1101 90-91 -8.4570732852535 1101 92-93 -14.18451604684137 1101 94-95 -12.204093568776642 1101 96-97 -12.083505969990355 1101 98-99 -11.598455821814213 1101 100-101 -11.390488661104982 1102 1 0.5901143475826593 1102 2 0.6224664084778695 1102 3 0.5241891319843539 1102 4 0.4700045661068728 1102 5 0.5987751124631231 1102 6 0.6208168676609205 1102 7 0.6512615768967223 1102 8 0.617211588774488 1102 9 0.7289086218883654 1102 10-11 0.7076052523413381 1102 12-13 0.7890048819672373 1102 14-15 0.8938967746290061 1102 16-17 0.8618347372088451 1102 18-19 0.8905695949253527 1102 20-21 0.9789015449499772 1102 22-23 0.9833820700223441 1102 24-25 0.8921772099425027 1102 26-27 0.9857811386066615 1102 28-29 0.9774468898634083 1102 30-31 1.0414117912890148 1102 32-33 0.9280305851635546 1102 34-35 0.9865851321181012 1102 36-37 1.122578782464835 1102 38-39 1.0895218883877007 1102 40-41 1.1180151879663924 1102 42-43 1.060511177967257 1102 44-45 1.2074599683975222 1102 46-47 1.1264387037729406 1102 48-49 1.1259728257882031 1102 50-51 1.0767447122438583 1102 52-53 1.0206021632222857 1102 54-55 1.127511266406458 1102 56-57 1.8451633575803825 1102 58-59 -9.262118204927646 1102 60-61 -9.885202887165118 1102 62-63 -2.649416047649911 1102 64-65 0.2532993658679459 1102 66-67 -1.0896948054961157 1102 68-69 -3.760996013854502 1102 70-71 -4.223522642932588 1102 72-73 -6.477137750078539 1102 74-75 -7.6642510240998885 1102 76-77 -8.496772410877874 1102 78-79 -11.532906268885469 1102 80-81 -7.4320219174125235 1102 82-83 -3.1161217735071354 1102 84-85 -2.1159059879030444 1102 86-87 -2.085216465543624 1102 88-89 -2.292139584762655 1102 90-91 -8.213084013448078 1102 92-93 -14.353397546829123 1102 94-95 -12.4591888488609 1102 96-97 -12.51226145043222 1102 98-99 -11.847164397021794 1102 100-101 -11.621980688439834 1103 1 0.258147196149384 1103 2 0.2226229314025474 1103 3 0.16203089509824053 1103 4 0.13498707679478628 1103 5 0.2344965565464605 1103 6 0.20243808395382246 1103 7 0.17435512678884635 1103 8 0.3405407198332213 1103 9 0.3234131002323437 1103 10-11 0.27404117753966517 1103 12-13 0.34417567167320584 1103 14-15 0.3984366476931669 1103 16-17 0.378821192841059 1103 18-19 0.35931337008477016 1103 20-21 0.2943006607016869 1103 22-23 0.3762589268507952 1103 24-25 0.26000630426084115 1103 26-27 0.09244535886487171 1103 28-29 0.41855525011756356 1103 30-31 0.3131768218720694 1103 32-33 0.28348358977514465 1103 34-35 0.3569326032724689 1103 36-37 -0.0011616417937432288 1103 38-39 0.06776836684321808 1103 40-41 0.2957665343025724 1103 42-43 0.3405451207779535 1103 44-45 0.19001680312860714 1103 46-47 -0.03126577864346558 1103 48-49 -0.02133036301226099 1103 50-51 0.060375871460784936 1103 52-53 0.11282917989357344 1103 54-55 0.07580004943186225 1103 56-57 0.6575130207639361 1103 58-59 -6.421753771221038 1103 60-61 -8.835618075731862 1103 62-63 -2.8636674245293143 1103 64-65 -0.5271213470373155 1103 66-67 -2.080298228815373 1103 68-69 -4.483320649305703 1103 70-71 -5.229618892136916 1103 72-73 -7.872367983968761 1103 74-75 -8.38148589790328 1103 76-77 -9.214229718984576 1103 78-79 -12.33542997971822 1103 80-81 -8.826577544579873 1103 82-83 -5.0923027115291895 1103 84-85 -4.1121596851816875 1103 86-87 -3.857448829491293 1103 88-89 -3.8338494472119358 1103 90-91 -9.192246235117473 1103 92-93 -14.728673321099695 1103 94-95 -12.755175315116842 1103 96-97 -12.541576963642385 1103 98-99 -12.075175604169438 1103 100-101 -11.705587767206808 1104 1 0.13432639976737448 1104 2 0.182481209763818 1104 3 0.1216773266008957 1104 4 0.22559323972833312 1104 5 0.06103437939209755 1104 6 0.14335742691868347 1104 7 0.1920712004484173 1104 8 0.21686226484698778 1104 9 0.1987145077758825 1104 10-11 0.26634446115149757 1104 12-13 0.14425993256659098 1104 14-15 0.2160025461120867 1104 16-17 0.3476504544092265 1104 18-19 0.3138481816062324 1104 20-21 0.3427373525196842 1104 22-23 0.35284007476826673 1104 24-25 0.25016252068463984 1104 26-27 0.4954049449134956 1104 28-29 0.3685572918405384 1104 30-31 0.24500246104719992 1104 32-33 0.3611358168749774 1104 34-35 0.337636583560716 1104 36-37 0.2650530414669703 1104 38-39 0.37855306614231665 1104 40-41 0.4223797850556963 1104 42-43 0.3657635127343255 1104 44-45 0.3392690691515057 1104 46-47 0.4206040775902764 1104 48-49 0.4305512946698613 1104 50-51 0.4573889755573788 1104 52-53 0.5173588653871448 1104 54-55 0.441904074203606 1104 56-57 0.8929539431477664 1104 58-59 -6.798198036817627 1104 60-61 -8.54470201979618 1104 62-63 -2.290315123927453 1104 64-65 0.057505680856962726 1104 66-67 -1.5674538364871538 1104 68-69 -4.092658968883715 1104 70-71 -4.704644638118776 1104 72-73 -7.451129836822222 1104 74-75 -8.578709096741612 1104 76-77 -8.91693755045468 1104 78-79 -12.072096153089172 1104 80-81 -8.226756767311866 1104 82-83 -4.309528496549955 1104 84-85 -3.3864448938979024 1104 86-87 -3.1504286908626504 1104 88-89 -3.1142122570397746 1104 90-91 -9.144561867533199 1104 92-93 -15.289231765636941 1104 94-95 -12.76648399872392 1104 96-97 -12.351543163715016 1104 98-99 -11.647423419525861 1104 100-101 -11.212023683459677 1105 1 0.12789460558478893 1105 2 0.22902398269016544 1105 3 0.07200167032025817 1105 4 0.01963497793369129 1105 5 0.07970578190721511 1105 6 0.22874551417201872 1105 7 0.13722311952143684 1105 8 0.17542503346133742 1105 9 0.09401254676322424 1105 10-11 0.1265021164343949 1105 12-13 0.303436019259685 1105 14-15 0.2464986849897386 1105 16-17 0.30420279555398366 1105 18-19 0.3206488164449368 1105 20-21 0.24834581567181147 1105 22-23 0.28638934873247734 1105 24-25 0.3062626926922505 1105 26-27 0.30474186580416784 1105 28-29 0.24687312016025942 1105 30-31 0.3089287432750254 1105 32-33 0.3538089693668809 1105 34-35 0.33450645421665826 1105 36-37 0.18040165195078117 1105 38-39 0.4515634511214728 1105 40-41 0.17544086742892517 1105 42-43 0.36843116507436235 1105 44-45 0.20923965632221808 1105 46-47 0.22763195761614696 1105 48-49 0.1496890691463264 1105 50-51 0.22307089996530038 1105 52-53 0.18376698295033833 1105 54-55 0.18142447512455107 1105 56-57 0.7627742400458075 1105 58-59 -7.084422123548492 1105 60-61 -8.91911738276686 1105 62-63 -3.144472971204209 1105 64-65 -0.6321273984254852 1105 66-67 -1.870070972410936 1105 68-69 -4.064394168439295 1105 70-71 -4.801502981274746 1105 72-73 -6.512495479679028 1105 74-75 -6.931198800677645 1105 76-77 -8.755376490900389 1105 78-79 -12.122955050194626 1105 80-81 -8.157860676904793 1105 82-83 -4.374604381334752 1105 84-85 -3.553592856638506 1105 86-87 -3.3368858062021722 1105 88-89 -3.367558182757037 1105 90-91 -8.613463583989748 1105 92-93 -11.432922446223687 1105 94-95 -11.806181089072767 1105 96-97 -11.870574357995489 1105 98-99 -11.39579206189324 1105 100-101 -11.068946950439724 1106 1 0.16030385239390554 1106 2 0.2422667462568242 1106 3 0.1248752079185067 1106 4 0.14441089856070022 1106 5 0.24206100448204637 1106 6 0.13981783531948366 1106 7 0.20880871268746404 1106 8 0.25651265079478947 1106 9 0.1880094206574583 1106 10-11 0.2786151633287659 1106 12-13 0.3003361036812535 1106 14-15 0.30487759575641604 1106 16-17 0.20981557636716275 1106 18-19 0.2634701965565469 1106 20-21 0.2139480646848071 1106 22-23 0.27922549565387556 1106 24-25 0.2788550758045858 1106 26-27 0.3278751878069883 1106 28-29 0.40946962683457855 1106 30-31 0.39695313915926533 1106 32-33 0.5101669168360417 1106 34-35 0.41299274476617853 1106 36-37 0.43469990807412984 1106 38-39 0.49259149970148997 1106 40-41 0.4082592663166764 1106 42-43 0.40891033412089683 1106 44-45 0.43092764199005984 1106 46-47 0.34629929482600375 1106 48-49 0.4817476738778339 1106 50-51 0.4510084502908711 1106 52-53 0.41346763464030545 1106 54-55 0.3643282707519049 1106 56-57 1.1451662124736686 1106 58-59 -6.743566958090565 1106 60-61 -9.016628200151576 1106 62-63 -2.6158863777957784 1106 64-65 0.04337139933330292 1106 66-67 -1.594862913633463 1106 68-69 -3.9902966852051964 1106 70-71 -4.702948178300208 1106 72-73 -6.129885209223513 1106 74-75 -6.730777250051169 1106 76-77 -8.528915203624642 1106 78-79 -12.185220836053597 1106 80-81 -8.138731667924901 1106 82-83 -4.101413663807108 1106 84-85 -3.0402418495699415 1106 86-87 -2.814806082146479 1106 88-89 -2.7037974663717286 1106 90-91 -8.384181975354785 1106 92-93 -12.089783161773138 1106 94-95 -12.061270066067191 1106 96-97 -12.290603027895994 1106 98-99 -11.86458059839094 1106 100-101 -11.450251194086512 1107 1 0.1050016821626798 1107 2 0.12764205925029515 1107 3 0.09101249984674453 1107 4 0.11948910218934827 1107 5 -0.02003403672786419 1107 6 0.07107623728828827 1107 7 0.02360963523974391 1107 8 0.12457708056327732 1107 9 0.04066046998193684 1107 10-11 0.1593477935333354 1107 12-13 0.244885471824567 1107 14-15 0.2079583534404108 1107 16-17 0.22040073278157735 1107 18-19 0.18365897331579362 1107 20-21 0.13840042453745838 1107 22-23 0.08880420536916489 1107 24-25 0.34685364988579437 1107 26-27 0.5441776200293162 1107 28-29 0.3998088838392633 1107 30-31 0.39893539534647715 1107 32-33 0.3545068352608922 1107 34-35 0.23260327995817676 1107 36-37 0.3173224533391732 1107 38-39 0.35137042072763336 1107 40-41 0.1327202160415908 1107 42-43 0.42615808178481274 1107 44-45 0.37254113496138785 1107 46-47 0.15920829801427772 1107 48-49 0.09363705922952903 1107 50-51 0.13597130625159792 1107 52-53 0.21121164785724744 1107 54-55 0.2442365595491367 1107 56-57 0.8708739821562901 1107 58-59 -6.820693130429174 1107 60-61 -9.34156288133131 1107 62-63 -2.959584919092304 1107 64-65 -0.46668046917865524 1107 66-67 -1.897733935269919 1107 68-69 -4.597845751271336 1107 70-71 -4.8795436396980385 1107 72-73 -6.381388365285744 1107 74-75 -7.115268665189674 1107 76-77 -9.430282138629373 1107 78-79 -12.638594293634661 1107 80-81 -8.572794520020185 1107 82-83 -4.391705247020777 1107 84-85 -3.3926097194391005 1107 86-87 -3.239161949100815 1107 88-89 -3.430854132327344 1107 90-91 -8.760192323287562 1107 92-93 -11.967212270273246 1107 94-95 -12.225023095786717 1107 96-97 -12.56283111191067 1107 98-99 -11.790763244844683 1107 100-101 -11.230830495071341 1108 1 0.019536711843358034 1108 2 0.026299747477633417 1108 3 -0.03824159592507215 1108 4 -0.051264090609542734 1108 5 -0.17655042386434872 1108 6 -0.013832777054005874 1108 7 0.033713213927967445 1108 8 0.009594208704449159 1108 9 -0.012881572923447493 1108 10-11 -0.01330386947554274 1108 12-13 -0.10653833021784465 1108 14-15 0.024534587840847166 1108 16-17 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62-63 1.5059528883663198 2314 64-65 1.4178004426519735 2314 66-67 1.8407679406458755 2314 68-69 0.02213502063925077 2314 70-71 2.757434379755594 2314 72-73 8.58837571091733 2314 74-75 10.15052976945248 2314 76-77 9.079280741285817 2314 78-79 6.657873399264197 2314 80-81 3.634473481348259 2314 82-83 2.05361079191686 2314 84-85 1.7107012975650733 2314 86-87 1.6800135326550958 2314 88-89 1.7123233702005187 2314 90-91 2.9869762418238572 2314 92-93 5.121305002542737 2314 94-95 8.941914994245849 2314 96-97 9.758721093073518 2314 98-99 9.989259476499518 2314 100-101 9.164794753870524 2315 1 -0.7181098029085895 2315 2 -0.5711806667551507 2315 3 -0.5552192766839497 2315 4 -0.4355653342988788 2315 5 -0.6758121381626481 2315 6 -0.7602498066239676 2315 7 -0.7897819990517689 2315 8 -0.8455762686762611 2315 9 -1.0382743893650357 2315 10-11 -0.9119704407137874 2315 12-13 -0.7706457945560601 2315 14-15 -0.9310721993225641 2315 16-17 -1.0486959699665945 2315 18-19 -0.7976827920007707 2315 20-21 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0.6463897300872716 2315 88-89 0.8059193231372497 2315 90-91 2.1451958452899227 2315 92-93 4.143207127958231 2315 94-95 8.207252877204088 2315 96-97 9.18118249556019 2315 98-99 9.331819876951867 2315 100-101 8.545236163682919 2316 1 -0.42428951262212067 2316 2 -0.4125138045012271 2316 3 -0.5421511122043441 2316 4 -0.43021314071460637 2316 5 -0.612372402505251 2316 6 -0.5323118620354776 2316 7 -0.6115611399918421 2316 8 -0.551783951293821 2316 9 -0.6824919751936633 2316 10-11 -0.5708342509498863 2316 12-13 -0.6661507362573147 2316 14-15 -0.8052549622317215 2316 16-17 -0.9218779988881707 2316 18-19 -0.9083801390987318 2316 20-21 -0.6276999970160304 2316 22-23 -0.763713106044122 2316 24-25 -2.237484807242623 2316 26-27 -1.4916840564037699 2316 28-29 -1.0578193009449635 2316 30-31 -0.6137960633482891 2316 32-33 -0.7789400830988527 2316 34-35 -0.6636536511040916 2316 36-37 -0.7293739667243457 2316 38-39 -0.9597547364788213 2316 40-41 -0.7670386684363777 2316 42-43 -1.0010108357133944 2316 44-45 -0.8827023942752632 2316 46-47 -0.8072342764466569 2316 48-49 -0.8869908137011961 2316 50-51 -0.9363234827235871 2316 52-53 -0.8018217220427459 2316 54-55 -0.744728964888445 2316 56-57 0.40971293423400823 2316 58-59 3.9464212087682107 2316 60-61 3.8263669823430355 2316 62-63 1.353137502938413 2316 64-65 0.9939724386583961 2316 66-67 6.25896093340117 2316 68-69 8.881359782238349 2316 70-71 5.524979279565766 2316 72-73 9.527980095929838 2316 74-75 10.465426283807314 2316 76-77 9.013400051936745 2316 78-79 6.285049832691211 2316 80-81 3.196654881354963 2316 82-83 1.9244903843526266 2316 84-85 1.4278064671752944 2316 86-87 1.3588719759929226 2316 88-89 1.3882446504202264 2316 90-91 2.9521078561857053 2316 92-93 4.956986754475796 2316 94-95 8.997050757580709 2316 96-97 9.810981521621297 2316 98-99 10.171375396875801 2316 100-101 9.11459116472956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 41.0 5 84.0 6 226.0 7 527.0 8 1288.0 9 2352.0 10 3875.0 11 5619.0 12 8069.0 13 10847.0 14 14263.0 15 19767.0 16 26210.0 17 33942.0 18 37539.0 19 39034.0 20 40480.0 21 40644.0 22 38897.0 23 37391.0 24 39149.0 25 43045.0 26 57272.0 27 81258.0 28 112171.0 29 146161.0 30 175132.0 31 194068.0 32 207246.0 33 217471.0 34 221204.0 35 213482.0 36 176626.0 37 106991.0 38 32986.0 39 1348.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.67422403561826 19.456565104568284 15.219269865962168 23.64994099385129 2 15.925893026241472 20.3856609642823 39.51795102999911 24.170494979477112 3 19.64824276411332 24.025599104349787 29.5674767016139 26.758681429922998 4 13.589763034922777 17.21184234617926 36.344058359516374 32.85433625938159 5 14.001729043315805 36.95905554991879 34.2929160374218 14.74629936934361 6 32.59435636225905 36.26269156496166 17.042111715519564 14.100840357259726 7 28.763874148780566 32.02240058858946 20.454279780267758 18.75944548236222 8 26.11997799491436 35.68393624558547 19.26136318405042 18.93472257544975 9 25.800941290631545 17.036916307422352 19.754533726787734 37.407608675158365 10-11 25.144748678418065 26.584442350452903 27.152019735847016 21.118789235282016 12-13 26.07175193769868 23.50597450981953 27.48530097543787 22.93697257704392 14-15 22.652739033266602 25.170443261415887 25.579414912025634 26.597402793291874 16-17 22.506046994142178 28.22114254565779 26.385885584544166 22.88692487565587 18-19 22.45065512100093 26.90528519231498 28.438534183127178 22.205525503556903 20-21 23.064700009175763 26.0981689119349 28.628898284384068 22.20823279450527 22-23 22.698046652250806 25.979721987008965 28.557440473821217 22.76479088691901 24-25 22.67171347491938 26.185338642146576 28.577363187935926 22.56558469499812 26-27 22.428912332979362 26.469082968938313 28.388978423722783 22.713026274359542 28-29 22.447172789731308 26.699348151034545 28.293178054383244 22.560301004850896 30-31 22.64259374450692 26.45903994780699 28.21278121152227 22.68558509616382 32-33 22.40210699820213 26.375584328739986 28.20590494123089 23.016403731826994 34-35 22.83117152466955 26.535055238462352 28.11267452201211 22.521098714855988 36-37 22.94177626216251 26.140668356157665 28.172814226257405 22.744741155422414 38-39 22.47868489986319 26.500878712894625 28.13922198443902 22.881214402803167 40-41 22.859128163659427 26.297875425668188 28.227277842120298 22.615718568552094 42-43 22.59329232363076 26.332109859511455 28.053746235492028 23.020851581365758 44-45 22.647345165566467 26.447006618802966 28.14191974203726 22.76372847359331 46-47 22.909557181166225 26.612046259843964 27.610959314027596 22.867437244962215 48-49 22.687289519515133 26.691110046128376 27.594608430597994 23.0269920037585 50-51 22.701311458852604 26.840179795189716 27.561799619849385 22.89670912610829 52-53 22.94048204747389 26.620165297845393 27.52248518379139 22.916867470889322 54-55 22.64880564510418 26.701059483705457 27.65094070402887 22.999194167161495 56-57 22.61358569352715 26.734387049053908 27.716115765342792 22.93591149207615 58-59 21.1181290333753 26.38215501085704 29.407447829838713 23.092268125928953 60-61 22.437632782292948 26.50391316300768 28.044818306322185 23.013635748377187 62-63 22.391587937481447 26.651919761697613 27.82654656577208 23.129945735048857 64-65 21.84199191373023 26.664444141860304 28.121848348978073 23.37171559543139 66-67 21.920188275599724 26.25477551796309 28.789406860840767 23.035629345596423 68-69 22.554524254385065 25.354864515489105 29.65371209123655 22.436899138889277 70-71 21.61321641519661 25.7201675209101 29.72050973288597 22.94610633100732 72-73 21.521366761789565 25.911639530506765 29.519765381560457 23.047228326143216 74-75 22.05468654661714 26.181744841975775 28.791377015754183 22.972191595652898 76-77 20.581388052474885 26.51497871207413 29.387152793971378 23.516480441479615 78-79 21.78789123456146 26.785016892545897 27.927662532888704 23.49942934000394 80-81 22.485766036331857 26.85713998410371 27.032568951293428 23.624525028271005 82-83 22.58721444829562 26.893568258497286 26.96087827738306 23.558339015824036 84-85 22.447126225844485 26.887190883563317 26.985212354015253 23.680470536576944 86-87 22.52161714740387 26.87751896279592 26.873035685629254 23.727828204170955 88-89 22.573517689432972 26.843915387698146 26.69241048861377 23.890156434255108 90-91 22.16355625514208 26.883077449015975 27.10043597698364 23.852930318858302 92-93 22.123934293975463 26.691514164605895 27.24931313996666 23.93523840145198 94-95 20.66541009144855 26.543141188882114 28.745389773323797 24.046058946345543 96-97 20.45977577629941 26.38418052708365 29.008622812664004 24.14742088395294 98-99 20.83907207463913 26.484913867014754 28.238862325673164 24.437151732672955 100-101 20.889573527218193 26.601242657839492 28.078163779803877 24.431020035138438 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 207.0 1 161.5 2 121.0 3 140.5 4 155.5 5 183.5 6 257.5 7 419.5 8 616.0 9 791.0 10 903.5 11 1020.5 12 1183.0 13 1357.5 14 1580.5 15 1811.0 16 1999.0 17 2192.0 18 2488.0 19 2790.5 20 3159.5 21 3790.5 22 4607.5 23 5617.0 24 6852.5 25 8532.0 26 10831.0 27 13487.5 28 16575.0 29 19967.5 30 23803.0 31 28868.5 32 34514.5 33 39609.5 34 44329.0 35 49086.0 36 54519.5 37 59586.5 38 63699.0 39 67958.5 40 72497.5 41 77145.0 42 81391.5 43 84964.0 44 88517.0 45 91771.0 46 93791.5 47 95120.0 48 95697.0 49 95520.0 50 95047.0 51 93876.0 52 91741.0 53 88825.0 54 85143.5 55 79585.5 56 72222.0 57 64100.0 58 55676.5 59 47016.5 60 38323.5 61 30609.0 62 23875.5 63 17795.5 64 12919.0 65 9502.0 66 7010.0 67 5157.0 68 3748.5 69 2640.5 70 1861.0 71 1274.5 72 859.5 73 620.0 74 447.5 75 299.5 76 193.5 77 109.5 78 69.0 79 41.5 80 20.5 81 11.0 82 7.0 83 5.0 84 3.0 85 1.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022248078222734686 2 0.0049859158352267945 3 0.003226180834558514 4 0.0 5 0.0665766408586166 6 0.0 7 0.0 8 8.379690479372763E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.094922619843191E-4 16-17 0.0 18-19 0.0011103089885168911 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.885430357858872E-4 28-29 0.0021158718460416227 30-31 0.00823304589598374 32-33 4.189845239686382E-4 34-35 0.0010684105361200274 36-37 0.02513907143811829 38-39 0.023567879473235895 40-41 0.026039888164650863 42-43 0.026018938938452432 44-45 0.02689880643878657 46-47 0.028469998403668966 48-49 0.027841521617716004 50-51 0.02360977792563276 52-53 0.020320749412478953 54-55 0.015586224291633338 56-57 0.16279643678801436 58-59 0.8016849881615922 60-61 0.3280229838150468 62-63 3.142383929764786E-4 64-65 0.1486347598778744 66-67 1.4780307559779664 68-69 2.8455543437592046 70-71 2.297501637182027 72-73 1.9374682357357766 74-75 1.6339348973466967 76-77 1.1777235984234449 78-79 0.4410650083817854 80-81 8.379690479372763E-5 82-83 2.3044148818275102E-4 84-85 4.189845239686382E-5 86-87 0.00318428238216165 88-89 6.284767859529572E-5 90-91 0.23605588080393072 92-93 0.18114795893784072 94-95 1.3480827058690934 96-97 1.322943634430975 98-99 1.0412603389668595 100-101 0.4241589828396508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2386723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.25349511402791 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21651330806436 47.12818302443755 2 12.789832677141874 15.41264241442805 3 3.9822256113808225 7.198290342548737 4 1.7299256879647598 4.16936275949664 5 0.8884462168060054 2.676599489169861 6 0.5467240770581958 1.976522190344845 7 0.3651012019211291 1.5399036440256328 8 0.24784735076475076 1.1946935310662936 9 0.1834719896627834 0.9949345769436748 >10 0.9624370968350267 10.37767348450442 >50 0.05901673134171297 2.420864919632747 >100 0.025602598067881295 2.8415209831182735 >500 0.0018107750672701815 0.7778804414805898 >1k 9.053875336319531E-4 0.8117870820739438 >5k 1.3929038978953125E-4 0.47914111672888543 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6189 0.25930952188419015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.21908700758320088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3356 0.14061120624387496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.379690479372763E-5 2 0.0 0.0 0.0 0.0 8.379690479372763E-5 3 0.0 0.0 0.0 0.0 8.379690479372763E-5 4 0.0 0.0 0.0 0.0 8.379690479372763E-5 5 0.0 0.0 0.0 0.0 1.6759380958745527E-4 6 0.0 0.0 0.0 0.0 2.513907143811829E-4 7 0.0 0.0 0.0 0.0 2.513907143811829E-4 8 0.0 0.0 0.0 0.0 2.513907143811829E-4 9 0.0 0.0 0.0 4.189845239686382E-5 2.513907143811829E-4 10-11 0.0 8.379690479372763E-5 0.0 4.189845239686382E-5 2.513907143811829E-4 12-13 0.0 8.379690479372763E-5 0.0 4.189845239686382E-5 2.723399405796148E-4 14-15 0.0 8.379690479372763E-5 0.0 8.379690479372763E-5 3.3518761917491054E-4 16-17 0.0 8.379690479372763E-5 0.0 1.6759380958745527E-4 4.189845239686382E-4 18-19 0.0 8.379690479372763E-5 0.0 1.6759380958745527E-4 4.3993375016707005E-4 20-21 0.0 8.379690479372763E-5 0.0 3.3518761917491054E-4 5.446798811592296E-4 22-23 0.0 8.379690479372763E-5 0.0 5.446798811592296E-4 7.122736907466849E-4 24-25 0.0 8.379690479372763E-5 0.0 9.636644051278678E-4 7.541721431435487E-4 26-27 0.0 8.379690479372763E-5 0.0 0.0016549888696761206 8.589182741357082E-4 28-29 0.0 8.379690479372763E-5 0.0 0.004650728216051884 8.798675003341401E-4 30-31 0.0 1.2569535719059144E-4 0.0 0.017366908518500052 9.00816726532572E-4 32-33 0.0 1.6759380958745527E-4 0.0 0.04598355150555804 9.846136313262997E-4 34-35 0.0 1.6759380958745527E-4 0.0 0.08913895747432776 0.0010474613099215955 36-37 0.0 1.6759380958745527E-4 0.0 0.1629430813714034 0.0010474613099215955 38-39 0.0 1.8854303578588717E-4 0.0 0.27787053629600084 0.0010474613099215955 40-41 0.0 2.094922619843191E-4 0.0 0.40748339878569906 0.0010893597623184593 42-43 0.0 2.513907143811829E-4 0.0 0.5383322656211047 0.001173156667112187 44-45 0.0 2.9328916677804674E-4 0.0 0.6967712633598453 0.0012150551195090507 46-47 0.0 2.9328916677804674E-4 0.0 0.8782963083692578 0.0012150551195090507 48-49 0.0 2.9328916677804674E-4 0.0 1.062670448141657 0.0012150551195090507 50-51 0.0 2.9328916677804674E-4 0.0 1.2506478548201865 0.0012150551195090507 52-53 0.0 2.9328916677804674E-4 0.0 1.4285277344710718 0.0012988520243027784 54-55 0.0 2.9328916677804674E-4 0.0 1.6229784520449169 0.0013407504766996421 56-57 0.0 2.9328916677804674E-4 0.0 1.7962495019321472 0.001361699702898074 58-59 0.0 2.9328916677804674E-4 0.0 1.9193262058479346 0.0013826489290965059 60-61 0.0 2.9328916677804674E-4 0.0 2.016551564634857 0.0014035981552949378 62-63 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.0951321121051754 0.0014454966076918017 64-65 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.1790966107084904 0.0014664458338902336 66-67 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.2503030305569602 0.0014664458338902336 68-69 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.3310203990995184 0.0014664458338902336 70-71 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.4758633490354764 0.0015083442862870973 72-73 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.6642806894641735 0.0015292935124855294 74-75 4.189845239686382E-5 2.9328916677804674E-4 0.0 2.8778371013309885 0.0015502427386839613 76-77 4.189845239686382E-5 2.9328916677804674E-4 0.0 3.1830882762683395 0.0015502427386839613 78-79 4.189845239686382E-5 2.9328916677804674E-4 0.0 3.6129454486339636 0.0015502427386839613 80-81 4.189845239686382E-5 2.9328916677804674E-4 0.0 4.0889537663147335 0.001592141191080825 82-83 4.189845239686382E-5 2.9328916677804674E-4 0.0 4.551889766847682 0.0016759380958745527 84-85 4.189845239686382E-5 2.9328916677804674E-4 0.0 4.92960431520541 0.0016759380958745527 86-87 6.284767859529572E-5 3.3518761917491054E-4 0.0 5.268520896643641 0.0016968873220729846 88-89 8.379690479372763E-5 3.3518761917491054E-4 0.0 5.618938603264811 0.0017178365482714165 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3675 0.0 51.683502 1 GTATCAA 6665 0.0 39.95329 1 ATCAACG 8160 0.0 32.221 3 TCAACGC 8250 0.0 31.86883 4 TATCAAC 8410 0.0 31.322493 2 CAACGCA 8490 0.0 30.967947 5 AACGCAG 8870 0.0 29.675083 6 ACGCAGA 10500 0.0 24.889 7 CGCAGAG 10595 0.0 24.62139 8 TGGTATC 1900 0.0 22.321812 2 GTACATG 7950 0.0 22.231482 1 TACATGG 7730 0.0 22.129297 2 GTGGTAT 2135 0.0 22.075289 1 GCAGAGT 11960 0.0 21.102667 9 ACATGGG 7935 0.0 20.605234 3 TAAGGTG 1455 0.0 20.401564 5 GAGTACT 6915 0.0 20.088278 12-13 GTACTTT 7045 0.0 19.484064 14-15 AGGTAAG 1375 0.0 19.192245 2 AGAGTAC 10760 0.0 19.123745 10-11 >>END_MODULE