FastQCFastQC Report
Fri 27 May 2016
SRR939854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621780
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58660.22374112244353075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.17320293846165583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.11072630045236442No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26960.10283090114349792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39400.048.8303151
GTATCAA75400.046.166061
ATCAACG91700.037.2965773
TATCAAC92200.037.1935272
TCAACGC92850.036.834644
CAACGCA94550.036.025155
AACGCAG99950.034.314176
ACGCAGA110600.030.414437
CGCAGAG111100.030.0234788
GCAGAGT121450.026.1477959
TGGTATC21100.024.0775362
GTGGTAT23200.023.3169781
GTACATG82400.021.6358721
CAGAGTA113300.021.23991610-11
GAGTACT69100.021.20965812-13
AGAGTAC107450.021.1265210-11
TACATGG82350.020.9068322
GTACTTT71300.020.52693414-15
ACATGGG84900.019.393863
AGTACTT73500.019.2038212-13
Produced by FastQC (version 0.11.5)