FastQCFastQC Report
Fri 27 May 2016
SRR939854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621780
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67030.255665997909817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56890.21698998390406518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.1385318371488073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.053.4237171
GTATCAA72900.044.2700921
ATCAACG93750.033.9026573
TATCAAC94750.033.603242
TCAACGC95050.033.389064
CAACGCA96600.032.8042075
AACGCAG101150.031.2816876
ACGCAGA116500.027.037867
CGCAGAG117450.026.6979968
TGGTATC19150.024.2826022
GCAGAGT131000.023.1398079
GTGGTAT23800.021.5365351
TAAGGTG14900.021.331375
AGAGTAC119250.021.0441610-11
GTAAGGT13850.020.8934564
AGTACTT80700.020.86859912-13
TACATGG86200.020.5323872
GTACATG87150.020.5306631
GAGTACT77650.020.31368412-13
CAGAGTA125000.019.56379110-11
Produced by FastQC (version 0.11.5)