FastQCFastQC Report
Fri 27 May 2016
SRR939853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800109
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159090.8837798155556137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111980.6220734411082884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84620.47008264499538643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51720.2873159347572841No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35030.19459932704075142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25570.1420469538233518No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25300.140547044651185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23580.1309920677025669No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19350.10749349067195377No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19210.10671575999008948No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.1042159113698115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA192400.028.9450871
TACATGG141900.027.4121632
GTACATG141500.027.2214621
GGTATCA133850.025.8357451
ACATGGG147350.025.2722233
TATCAAC228100.025.0179772
GAGTACT112800.024.30695512-13
ATCAACG235750.024.1062163
GTAAGGT4150.024.0053044
TCAACGC238000.023.9985794
CAACGCA240250.023.7935735
AGTACTT113650.023.7286412-13
AACGCAG241800.023.6606676
CATGGGG51500.023.489244
ATGGGAG31700.022.896455
CATGGGA94400.022.6139554
GTACTTT123650.022.37056214-15
GTACTAG1503.065985E-522.1369931
TCGACCG1205.989932E-921.75470218-19
ATGGGGA28450.021.6768975
Produced by FastQC (version 0.11.5)