FastQCFastQC Report
Fri 27 May 2016
SRR939853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800109
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173960.9663859244079108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140480.780397187059228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107840.5990748338017309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60610.336701833055665No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40160.22309760131192058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.17471164246165094No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.15782377622688404No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28280.1571015977365815No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23390.12993657606289397No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23130.12849221908228892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.10993778710067001No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.10449367232762015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194200.028.0335671
GGTATCA137300.027.2473581
TACATGG139300.026.6808872
GTACATG140950.026.6756481
ACATGGG138700.025.4013753
CATGGGG47700.023.6289464
AGTACTT123150.023.17250812-13
GAGTACT122950.023.13353512-13
CATGGGA89650.023.0403274
TATCAAC235650.022.9773332
GATAGTC3900.022.9602327
ATCAACG235650.022.9366653
TCAACGC237350.022.789724
CAACGCA240050.022.533395
AACGCAG242950.022.3277876
ATGGGAT32850.021.9644765
GTACTTT130250.021.89157514-15
ATGGGAG28700.021.6898635
ATGGGGA27150.021.5385595
TACTTTT138150.020.63971914-15
Produced by FastQC (version 0.11.5)