FastQCFastQC Report
Fri 27 May 2016
SRR939852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161920.8717543410049724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116860.6291576845963506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91150.4907386868984885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52260.2813604363940209No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35340.19026555342833332No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27490.14800226552758583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.13637313153196612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.13411191103281783No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21360.11499921395668362No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20310.10934616270881295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.10762332804279522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18890.10170108387835926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG143150.028.4915141
TACATGG143550.028.1171862
GTATCAA209350.026.6052021
ACATGGG150450.025.6394943
ATGGGAG32350.025.0129785
CATGGGA99500.023.8277784
AGTACTT110050.023.061912-13
GAGTACT109800.023.0501412-13
TATCAAC245300.022.7636012
CATGGGG51850.022.1369174
ATCAACG252400.022.048713
GGTATCA151950.021.9808981
TCAACGC252750.021.980364
AATACCG1303.1403895E-421.7129655
CAACGCA254800.021.6945025
AACGCAG260200.021.3889126
GTACTTT120650.021.11913314-15
ATGGGAT33800.020.5994785
ACTTTTT137550.019.70881716-17
TACTTTT130450.019.58667214-15
Produced by FastQC (version 0.11.5)