FastQCFastQC Report
Fri 27 May 2016
SRR939852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176790.951812314391484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144740.7792596548731455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109640.5902862274443256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62450.33622195279002304No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40820.21976909708388698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.17109901776888603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29800.16043897827290132No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29160.15699330894086586No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23750.12786663536850357No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23160.12469015895303337No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.11149970604133512No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.1035315957110031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146750.026.4527241
TACATGG146450.026.1469422
GTATCAA209100.026.099911
TCGACCG1209.094947E-1225.69796418-19
GGTATCA153900.024.8845671
ACATGGG148700.024.821913
ATGGGAG31050.023.0718255
GAGTACT120200.022.83313412-13
CATGGGA98400.022.515854
AGTACTT120200.022.2805612-13
TATCAAC246800.021.8793222
CATGGGG50250.021.7149264
GTACTTT129250.021.39954814-15
ATCAACG254250.021.2161253
TCAACGC255200.021.1371484
TACACCG1805.2262185E-621.0855065
CAACGCA256050.021.066985
TAAGGTA3650.020.7966634
AACGCAG260900.020.7117236
TAGCCGG1150.003750402720.6271295
Produced by FastQC (version 0.11.5)