##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939852_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857404 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.377327172763707 31.0 31.0 34.0 30.0 34.0 2 31.577303591464215 31.0 31.0 34.0 30.0 34.0 3 31.602811773852107 31.0 31.0 34.0 30.0 34.0 4 35.427457354458156 37.0 35.0 37.0 33.0 37.0 5 35.17879255132432 37.0 35.0 37.0 32.0 37.0 6 35.22498820935026 37.0 35.0 37.0 32.0 37.0 7 35.119644406924934 37.0 35.0 37.0 32.0 37.0 8 35.09034491150014 37.0 35.0 37.0 32.0 37.0 9 36.637134409100014 39.0 35.0 39.0 32.0 39.0 10-11 36.50197722197217 38.0 35.0 39.0 32.0 39.0 12-13 36.40411025280445 38.0 35.0 39.0 32.0 39.0 14-15 37.33618991883295 39.0 36.0 41.0 32.0 41.0 16-17 37.29143282775314 39.0 36.0 41.0 32.0 41.0 18-19 37.34168576141755 39.0 36.0 41.0 32.0 41.0 20-21 37.296286376038815 39.0 36.0 41.0 31.0 41.0 22-23 37.15418455004942 39.0 36.0 40.0 31.0 41.0 24-25 36.96786751832127 39.0 36.0 40.0 31.0 41.0 26-27 36.649221171053796 38.5 35.0 40.0 30.0 41.0 28-29 36.388744721126905 38.0 35.0 40.0 30.0 41.0 30-31 36.0405108420139 38.0 35.0 40.0 30.0 41.0 32-33 35.90912289410382 38.0 34.5 40.0 30.0 41.0 34-35 35.67824878163286 38.0 34.0 40.0 29.0 41.0 36-37 35.60879162530069 38.0 34.0 40.0 28.5 41.0 38-39 35.444769420115385 38.0 34.0 40.0 27.0 41.0 40-41 35.279677442279656 38.0 34.0 40.0 27.0 41.0 42-43 34.96382962457279 38.0 33.5 40.0 26.0 41.0 44-45 34.60625744318414 38.0 33.0 40.0 24.5 41.0 46-47 34.15389220654203 38.0 33.0 40.0 23.5 41.0 48-49 33.72678695641875 37.0 33.0 40.0 22.5 41.0 50-51 33.438694005181425 37.0 33.0 40.0 21.0 41.0 52-53 33.13977196129652 37.0 32.0 40.0 20.0 41.0 54-55 32.84431846814156 36.5 32.0 40.0 18.5 41.0 56-57 32.4625735704241 36.0 31.0 39.0 17.0 41.0 58-59 32.07742984294208 35.5 31.0 39.0 13.5 40.5 60-61 31.727428173946002 35.0 31.0 39.0 12.5 40.0 62-63 31.419780241670633 35.0 30.0 38.5 8.0 40.0 64-65 30.949417035819884 35.0 29.5 38.0 7.5 40.0 66-67 30.042657655523517 34.5 28.5 37.5 4.0 40.0 68-69 28.222188603017976 33.0 24.5 36.0 2.0 39.0 70-71 27.580968114637418 33.0 23.5 36.0 2.0 39.0 72-73 28.102769779757125 33.0 25.5 35.5 2.0 38.5 74-75 28.185880400817485 33.0 26.0 35.0 2.0 38.0 76-77 27.704212976821413 32.5 26.0 35.0 2.0 37.0 78-79 28.206462083639316 33.0 27.0 35.0 2.0 37.0 80-81 28.023824380694776 33.0 27.0 35.0 2.0 36.5 82-83 27.595080822481272 33.0 26.0 35.0 2.0 36.0 84-85 27.25290701430599 33.0 26.0 35.0 2.0 36.0 86-87 26.869476430545 33.0 25.0 35.0 2.0 35.0 88-89 26.42245521168254 32.5 24.5 35.0 2.0 35.0 90-91 25.94155714104201 32.0 23.5 34.5 2.0 35.0 92-93 25.458447112206066 32.0 21.5 34.0 2.0 35.0 94-95 24.674766771257087 31.0 18.0 34.0 2.0 35.0 96-97 24.15463733253509 31.0 11.0 34.0 2.0 35.0 98-99 23.512905377613055 31.0 2.0 34.0 2.0 35.0 100-101 22.133090054721535 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30099506862637426 1101 2 0.2721193003208988 1101 3 0.33723881291671987 1101 4 0.2459051097054683 1101 5 0.1994560011272739 1101 6 0.22797239348274445 1101 7 0.161980468310297 1101 8 0.16530174036510203 1101 9 0.29275763857120296 1101 10-11 0.380921450318219 1101 12-13 0.2843966092576906 1101 14-15 0.40903010433961384 1101 16-17 0.5696777953772099 1101 18-19 0.4301574407850879 1101 20-21 0.4065141053708814 1101 22-23 0.3162839536616673 1101 24-25 0.28337532490699147 1101 26-27 0.1518586585372077 1101 28-29 0.20672207878705962 1101 30-31 0.40659082026095206 1101 32-33 0.44972013541948286 1101 34-35 0.1979731213080953 1101 36-37 0.3208317536106762 1101 38-39 0.41013403656600644 1101 40-41 0.4530742100427787 1101 42-43 0.29605990870076226 1101 44-45 0.10223649837918458 1101 46-47 0.2034443982556482 1101 48-49 0.32285099680959206 1101 50-51 0.2784804881502154 1101 52-53 0.06998170849645646 1101 54-55 0.05925766050811632 1101 56-57 0.08838858127800364 1101 58-59 0.17462574349066529 1101 60-61 0.12776597273941448 1101 62-63 0.19066667270944038 1101 64-65 -0.03902385642291861 1101 66-67 -0.13533336109468763 1101 68-69 -8.65008790470474 1101 70-71 -6.455009892611525 1101 72-73 -1.399719253140681 1101 74-75 -0.3718156838783564 1101 76-77 -0.21235539951538485 1101 78-79 -0.38181058341455554 1101 80-81 -0.37844075907246477 1101 82-83 -0.2679460563990368 1101 84-85 -0.06575736806440702 1101 86-87 -0.2751817370905094 1101 88-89 -0.07086954039750282 1101 90-91 -0.3749368781342426 1101 92-93 -0.8096641607705237 1101 94-95 -0.518233154361571 1101 96-97 -0.9453859907434854 1101 98-99 -0.5204672480528316 1101 100-101 -0.5679647955948077 1102 1 0.17404392663641843 1102 2 0.06166128072516841 1102 3 0.1716372872790224 1102 4 0.11731223591378637 1102 5 0.16086500752289368 1102 6 0.20472880178779462 1102 7 0.23372483446333803 1102 8 0.22883953660651457 1102 9 0.26923172033845333 1102 10-11 0.256002104384649 1102 12-13 0.11100573622405108 1102 14-15 0.2681949728906403 1102 16-17 0.2693405554324855 1102 18-19 0.29261054801843756 1102 20-21 0.15909443861169592 1102 22-23 0.20725845772495433 1102 24-25 0.19418684955982712 1102 26-27 0.2558686802339736 1102 28-29 0.039038917207349755 1102 30-31 0.002212968270484339 1102 32-33 0.09966611733984365 1102 34-35 -0.00453215366183457 1102 36-37 -0.20323076410933538 1102 38-39 0.20816177977420125 1102 40-41 0.12071379902786816 1102 42-43 0.25496916131138647 1102 44-45 -0.026872382372175707 1102 46-47 -0.08322044791219696 1102 48-49 0.1857435605195903 1102 50-51 0.07971633824855218 1102 52-53 -0.013761833955484803 1102 54-55 -0.1298432219861425 1102 56-57 -0.09899163139207445 1102 58-59 -0.24076925998619458 1102 60-61 0.045473755819521955 1102 62-63 0.1314662895093619 1102 64-65 -0.030848026081468305 1102 66-67 0.36409691230842256 1102 68-69 -4.165341744912695 1102 70-71 0.16884535684157953 1102 72-73 0.5168131144329493 1102 74-75 0.19586210573763907 1102 76-77 0.11116589285970946 1102 78-79 -0.026411610653397588 1102 80-81 0.024951039132698583 1102 82-83 -0.07401562929807781 1102 84-85 -0.2579895085233481 1102 86-87 -0.1903973766675584 1102 88-89 -0.15370208733503787 1102 90-91 -0.2576548231790561 1102 92-93 -0.32103183288973725 1102 94-95 -0.2837015746759519 1102 96-97 -0.7110870469312438 1102 98-99 -0.2701623891116469 1102 100-101 -0.22528155940448613 1103 1 0.2509368116891473 1103 2 0.2196149248343886 1103 3 0.21341905341563105 1103 4 0.23770935469526933 1103 5 0.32338458101826006 1103 6 0.16385231895832675 1103 7 0.20723015113688348 1103 8 0.3024897337317398 1103 9 0.30930155564303874 1103 10-11 0.376736399490035 1103 12-13 0.33554321635786977 1103 14-15 0.3079151926406283 1103 16-17 0.41259015079162253 1103 18-19 0.32147468527730183 1103 20-21 0.47132805614486983 1103 22-23 0.34177922538647465 1103 24-25 0.25163282671261555 1103 26-27 0.24121043712165147 1103 28-29 0.20375833206593796 1103 30-31 0.2761210233309086 1103 32-33 0.27326524044725176 1103 34-35 0.15809498259310573 1103 36-37 0.31587121124049133 1103 38-39 0.3867983522832148 1103 40-41 0.36839498008160376 1103 42-43 0.30760700993912593 1103 44-45 0.3551519048599019 1103 46-47 0.4211626979546068 1103 48-49 0.2798075596298162 1103 50-51 0.13578214616926942 1103 52-53 0.2095235278505143 1103 54-55 0.24750947557078717 1103 56-57 0.29948039204636956 1103 58-59 0.38443979226150304 1103 60-61 0.18422620342108686 1103 62-63 0.0653968522601609 1103 64-65 0.06830142322647248 1103 66-67 0.5157290857200785 1103 68-69 -3.5084580209875185 1103 70-71 0.7206281181166467 1103 72-73 0.8034664052680185 1103 74-75 0.5552797741282376 1103 76-77 0.3352703414212961 1103 78-79 0.20245062413488668 1103 80-81 0.09614324427171184 1103 82-83 0.07343769431802016 1103 84-85 0.4092042888371914 1103 86-87 0.2542271195436001 1103 88-89 -0.04113831798471779 1103 90-91 -0.030556471303953003 1103 92-93 -0.0038782800991015165 1103 94-95 0.08838684044898315 1103 96-97 -0.09595611305233831 1103 98-99 0.1881111627423806 1103 100-101 0.10451961778576901 1104 1 0.22179367851019904 1104 2 0.27877115215585846 1104 3 0.2919973269792173 1104 4 0.14445496267833846 1104 5 0.28965905705687334 1104 6 0.2339957171510889 1104 7 0.23356911387057266 1104 8 0.27526823026475 1104 9 0.3039562717148456 1104 10-11 0.365631398109052 1104 12-13 0.3250804821085822 1104 14-15 0.3667047895624407 1104 16-17 0.32826739527414617 1104 18-19 0.2946768404993563 1104 20-21 0.37787588219293156 1104 22-23 0.3397264865190053 1104 24-25 0.4607069221153566 1104 26-27 0.5392496423933011 1104 28-29 0.3252585003552966 1104 30-31 0.13413511938220068 1104 32-33 0.3643352253403478 1104 34-35 0.5193173392480261 1104 36-37 0.44344377414773106 1104 38-39 0.37531386118241983 1104 40-41 0.4312380583568398 1104 42-43 0.26483118031728026 1104 44-45 0.42054881616302 1104 46-47 0.31185610045245227 1104 48-49 0.5170376956143343 1104 50-51 0.4907787747909893 1104 52-53 0.43392118052139494 1104 54-55 0.44920665423594386 1104 56-57 0.41954311198267646 1104 58-59 0.4615217721047955 1104 60-61 0.46860853113695455 1104 62-63 0.5712092649705376 1104 64-65 0.5354801051573368 1104 66-67 0.4712015930526867 1104 68-69 -3.8268964410578974 1104 70-71 0.7416581384790994 1104 72-73 1.0323737656074137 1104 74-75 0.8378501956791169 1104 76-77 0.641122142788408 1104 78-79 0.2466809546865072 1104 80-81 0.44666664655709454 1104 82-83 0.40409051168324694 1104 84-85 0.4484438054162112 1104 86-87 0.6487706304511001 1104 88-89 0.3407184951626512 1104 90-91 0.3665956645173054 1104 92-93 0.321724877926286 1104 94-95 0.5201868769023612 1104 96-97 0.29250507919673296 1104 98-99 0.39110853049545824 1104 100-101 0.2885710136929305 1105 1 0.1766762940549853 1105 2 0.09802362003661358 1105 3 0.14729965940589196 1105 4 0.18757433124972067 1105 5 0.22410745640931395 1105 6 0.25042926833747003 1105 7 0.23375907664114237 1105 8 0.21019467079305088 1105 9 0.3122898321676999 1105 10-11 0.20786758969618546 1105 12-13 0.27585614070978437 1105 14-15 0.3404538152196821 1105 16-17 0.4064091396611147 1105 18-19 0.2670808643305591 1105 20-21 0.3103528386459402 1105 22-23 0.3394689329778515 1105 24-25 0.25207111099884827 1105 26-27 0.17879310489404787 1105 28-29 0.39301743024078206 1105 30-31 0.3859450802994786 1105 32-33 0.2603225684234687 1105 34-35 0.4053417209832588 1105 36-37 0.3179023666244376 1105 38-39 0.5136198402401817 1105 40-41 0.4856811976210835 1105 42-43 0.6323365085413357 1105 44-45 0.5975979339399373 1105 46-47 0.6225671935151311 1105 48-49 0.5286761176968966 1105 50-51 0.5322795079328202 1105 52-53 0.5290194869365195 1105 54-55 0.6775801968099628 1105 56-57 0.7531856004132038 1105 58-59 0.7028345865517736 1105 60-61 0.4350555732231065 1105 62-63 0.6508666233705931 1105 64-65 0.5365434333486867 1105 66-67 0.9686240135653961 1105 68-69 2.2474683964789612 1105 70-71 2.5575076290954293 1105 72-73 1.6726799408964474 1105 74-75 1.2514585616722371 1105 76-77 0.9690059248190757 1105 78-79 0.7876776187549268 1105 80-81 0.9210959406150998 1105 82-83 0.7156125577212826 1105 84-85 0.7234983141021694 1105 86-87 0.5250522659297125 1105 88-89 0.7087749062017537 1105 90-91 0.8114351909458435 1105 92-93 0.8002254257385815 1105 94-95 1.2197854204302736 1105 96-97 0.41652855608777983 1105 98-99 0.47359911411995625 1105 100-101 0.5316346471329183 1106 1 0.39340811466286496 1106 2 0.335157038959629 1106 3 0.34935669675280323 1106 4 0.2078807230677313 1106 5 0.4237367280796107 1106 6 0.3919219908105305 1106 7 0.3211716237034423 1106 8 0.30328931470632625 1106 9 0.4512653525577619 1106 10-11 0.3744847297507121 1106 12-13 0.4562688464495821 1106 14-15 0.6795858493209224 1106 16-17 0.5517440213021203 1106 18-19 0.596772308728525 1106 20-21 0.5657197442905328 1106 22-23 0.503935992972373 1106 24-25 0.5872354864586598 1106 26-27 0.5248449203088441 1106 28-29 0.5227088746387452 1106 30-31 0.5749660401540737 1106 32-33 0.7416671438169118 1106 34-35 0.6085931671122538 1106 36-37 0.7102115569364571 1106 38-39 0.7201926948483219 1106 40-41 0.8450020579416133 1106 42-43 0.8714093992125456 1106 44-45 0.969278328614152 1106 46-47 0.7912731064156091 1106 48-49 0.6877467003318074 1106 50-51 0.7406317142712595 1106 52-53 0.7126533458289543 1106 54-55 0.8069665476603234 1106 56-57 0.7234595354708304 1106 58-59 0.820686526023529 1106 60-61 0.9109207262026366 1106 62-63 0.813279531063575 1106 64-65 0.7599800993902583 1106 66-67 1.166934819502817 1106 68-69 2.5710695101064758 1106 70-71 2.9106018402519 1106 72-73 1.7977055456740842 1106 74-75 1.2742381586313982 1106 76-77 0.9177127032331676 1106 78-79 0.7670747885254237 1106 80-81 0.6938502934272996 1106 82-83 0.8189303598937236 1106 84-85 0.8502751414177787 1106 86-87 0.8238585784089771 1106 88-89 0.902470360812778 1106 90-91 0.8152008964115609 1106 92-93 0.7984244361680197 1106 94-95 1.1124566839358927 1106 96-97 1.1439004240104005 1106 98-99 1.405066303941517 1106 100-101 1.5794477827444737 1107 1 0.08926751665390498 1107 2 0.04350588042251147 1107 3 0.004951381504707086 1107 4 -0.0029666450018766 1107 5 -0.0029878678622452526 1107 6 -0.02763683314582721 1107 7 -0.08396681719969479 1107 8 0.028811970123911124 1107 9 0.04776288625709668 1107 10-11 -0.016412748206455774 1107 12-13 -0.14913916010012684 1107 14-15 -0.10597939659785283 1107 16-17 -0.01075586982644694 1107 18-19 -0.22397887506676994 1107 20-21 -0.20304170535425925 1107 22-23 -0.16048990623301762 1107 24-25 -0.15594495268512532 1107 26-27 -0.12657563403541872 1107 28-29 -0.16378061709138336 1107 30-31 -0.11714760419346959 1107 32-33 0.09901988432840625 1107 34-35 -0.09125702580791284 1107 36-37 -0.31239087655761466 1107 38-39 -0.06523929244710303 1107 40-41 -0.15107305463143916 1107 42-43 -0.18785134930027425 1107 44-45 -0.12956269878370819 1107 46-47 -0.014916016312973568 1107 48-49 0.035292302942394826 1107 50-51 -0.18832750394481934 1107 52-53 -0.10340286666483678 1107 54-55 -0.05848680746664314 1107 56-57 -0.17371503332574179 1107 58-59 -0.27022677458063527 1107 60-61 -0.13953696926511938 1107 62-63 0.020011123808959752 1107 64-65 -0.22964256676931072 1107 66-67 0.494496999602287 1107 68-69 1.5865626395937937 1107 70-71 1.7903039970673937 1107 72-73 0.8878242300427601 1107 74-75 0.45780829940941103 1107 76-77 0.11887661133987848 1107 78-79 0.10039191211336274 1107 80-81 0.04488907197701408 1107 82-83 0.06900976345250953 1107 84-85 0.09268027085864716 1107 86-87 -0.1843710851347744 1107 88-89 0.19056839154644933 1107 90-91 0.0869214713688109 1107 92-93 0.04914008433041417 1107 94-95 0.31551306772072607 1107 96-97 0.28045277084282105 1107 98-99 0.5235851880529694 1107 100-101 0.678255277743645 1108 1 -0.11321592833144578 1108 2 -0.17670693592947018 1108 3 -0.13765061181710436 1108 4 -0.073789939568222 1108 5 -0.027034088603855366 1108 6 0.005359450995619852 1108 7 -0.021719025728430097 1108 8 0.06775935651922538 1108 9 -0.17367992003683952 1108 10-11 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-0.7761249462594613 2316 66-67 -0.24631459064123717 2316 68-69 0.9938826466746136 2316 70-71 1.2068597355286563 2316 72-73 0.38848840541293583 2316 74-75 -0.07389481907864237 2316 76-77 -0.5390790666277603 2316 78-79 -0.6773153517210737 2316 80-81 -0.6612935434940184 2316 82-83 -0.6359592161427408 2316 84-85 -0.6310729541901772 2316 86-87 -0.6072191360274743 2316 88-89 -0.7069372919269412 2316 90-91 -0.6451292763686034 2316 92-93 -0.7890522993273876 2316 94-95 -0.2903343238751219 2316 96-97 -0.4856627162214373 2316 98-99 -0.6096588451711327 2316 100-101 -0.5833268116837687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 56.0 5 166.0 6 392.0 7 938.0 8 1715.0 9 3092.0 10 4897.0 11 7312.0 12 9866.0 13 12742.0 14 15516.0 15 19211.0 16 23505.0 17 27794.0 18 23774.0 19 18285.0 20 17501.0 21 17998.0 22 19028.0 23 21539.0 24 25105.0 25 29534.0 26 35730.0 27 43310.0 28 52060.0 29 62576.0 30 73571.0 31 87159.0 32 104017.0 33 125548.0 34 156929.0 35 199493.0 36 235296.0 37 242441.0 38 130309.0 39 8974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72027446699778 19.040690238618488 14.512103247152233 21.7269320472315 2 16.262389367425353 21.89419661969859 37.9232444862098 23.920169526666253 3 19.74067031189768 26.35377117740675 30.022547598691506 23.883010912004067 4 13.142482733966332 17.4579682180075 35.5271658723681 33.87238317565807 5 13.486672797086689 38.68415272067897 32.21447784111588 15.614696641118464 6 31.258752139411854 35.837887631278726 17.026461139558833 15.876899089750582 7 28.377132815477946 34.46008515110337 19.356424342792415 17.806357690626275 8 27.696236252317753 33.08946249711964 19.140854655206947 20.07344659535567 9 28.073375528425693 16.394871551907933 19.360839106624084 36.17091381304229 10-11 25.865132195257466 26.78714485378518 25.584094790363327 21.763628160594035 12-13 27.481043434815472 24.491171549108326 26.625225314471162 21.402559701605036 14-15 23.710727445402295 25.286044393142255 26.41665464271639 24.58657351873906 16-17 22.049283393646117 29.000880531689315 26.500477414537144 22.44935866012742 18-19 21.772484607549032 26.466374574406 30.348674817110332 21.41246600093464 20-21 23.011256570999095 25.327150151501776 30.071944498881237 21.5896487786179 22-23 22.53893067959367 25.744856800136105 30.05845793376131 21.65775458650891 24-25 22.509256878894046 25.84986291339653 30.249178624450007 21.39170158325942 26-27 22.149827137442895 26.241917130958026 30.21499671180233 21.393259019796755 28-29 21.517334018878515 26.68463082180626 30.53759214817467 21.26044301114056 30-31 21.529607547763053 26.391193486802507 30.326941237150034 21.75225772828441 32-33 21.353945699389026 26.533463553724523 29.965989434488876 22.14660131239757 34-35 22.325183225988816 26.21166050865823 29.80672602582998 21.656430239522972 36-37 22.252719566055074 25.93413759373981 29.875696227772586 21.93744661243253 38-39 21.9703905764585 26.12026881601644 30.151835459852467 21.757505147672596 40-41 21.707761100239665 26.144461696244132 30.310456943945184 21.837320259571023 42-43 21.81238825496529 26.34494875391234 29.57533181622817 22.2673311748942 44-45 21.61827896290161 26.488485381836092 29.398780299517224 22.494455355745078 46-47 21.91806252195899 26.781318638682738 28.689649151924957 22.610969687433315 48-49 22.283318900410677 26.70510117146474 28.351289613522795 22.660290314601788 50-51 21.743809243853025 27.05285061597037 28.465879070263707 22.737461069912897 52-53 22.25301863346006 26.99662975497233 28.07653050366724 22.673821107900373 54-55 21.243619334571108 26.685894968543465 28.534311556132213 23.536174140753214 56-57 21.92238269335323 27.141094884476676 27.93641928716206 23.000103135008036 58-59 22.221261958646924 26.363300556934927 27.959581698417292 23.455855786000864 60-61 21.789393853325045 26.45522288608942 28.679385887098185 23.075997373487354 62-63 21.98908130604378 26.59369590791255 28.249024828184012 23.168197957859658 64-65 21.617448161197782 26.735183837148195 28.13423385394681 23.513134147707206 66-67 21.43507093824842 26.948149550116458 28.220867165929146 23.395912345705973 68-69 21.25249127496242 26.657730108928153 28.590118805399023 23.49965981071041 70-71 21.154291020373307 27.056031729044854 28.119125527114864 23.670551723466975 72-73 21.28349451043181 26.88120628915654 27.88332789918536 23.951971301226283 74-75 21.422462749947087 27.34183773210767 27.264690660438173 23.97100885750707 76-77 21.473721342287664 26.999967686864174 27.55817710829748 23.96813386255069 78-79 21.336497047747265 27.127989638781454 27.51618536588255 24.01932794758873 80-81 21.216463440897265 27.64351429112899 27.37151766793584 23.768504600037904 82-83 21.376594094011907 27.44934728758379 26.915995074843746 24.258063543560553 84-85 21.106306000734897 27.169117003764676 26.848480198772744 24.87609679672768 86-87 21.541891512834468 26.91440259160614 27.028972515601268 24.514733379958123 88-89 21.278722946053623 26.94400775277269 26.784295251426727 24.99297404974696 90-91 21.087788391521986 27.13661759698071 27.050609927627033 24.724984083870265 92-93 21.252386117165855 27.11732818408509 26.866003520591203 24.764282178157853 94-95 20.872222024658154 26.81611670217432 27.08118482406201 25.23047644910552 96-97 21.040024080789106 26.70679054559788 26.96981070302765 25.283374670585356 98-99 21.051765741053366 26.816211183502336 26.543572859805316 25.58845021563898 100-101 20.764430770423626 26.719809047109322 26.981383923860065 25.53437625860699 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3805.0 1 2516.5 2 1598.0 3 2059.0 4 1844.0 5 1811.0 6 3203.0 7 6518.5 8 9390.5 9 9862.0 10 8017.5 11 5882.0 12 5229.5 13 5018.0 14 4917.0 15 4784.5 16 4560.5 17 4460.0 18 4505.5 19 4565.0 20 4844.0 21 5400.0 22 6004.5 23 6721.0 24 7579.5 25 8634.0 26 9760.5 27 10774.5 28 12005.0 29 13672.5 30 15798.0 31 18084.0 32 20691.0 33 22695.5 34 24207.5 35 25956.0 36 27963.5 37 31215.0 38 34696.5 39 36145.0 40 38112.0 41 44661.0 42 50899.5 43 54800.0 44 60162.0 45 65352.0 46 68346.0 47 73423.0 48 86896.0 49 89018.5 50 78661.0 51 72451.5 52 67920.5 53 67651.5 54 67768.0 55 63301.5 56 57717.5 57 52403.5 58 47163.0 59 42209.0 60 37217.0 61 31186.0 62 24723.5 63 18889.0 64 14202.0 65 11304.0 66 8976.0 67 7273.5 68 5117.5 69 3125.5 70 2295.0 71 1939.0 72 1662.0 73 1461.5 74 1218.0 75 979.0 76 632.0 77 322.0 78 187.0 79 103.5 80 60.0 81 43.0 82 27.0 83 17.0 84 12.5 85 9.0 86 7.0 87 3.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022773720741421897 2 0.02525029557382239 3 0.0 4 0.0 5 0.0 6 5.383858331305413E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8843504159568947E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.07578749695812E-5 26-27 1.8843504159568947E-4 28-29 8.07578749695812E-5 30-31 3.230314998783248E-4 32-33 0.002153543332522165 34-35 0.0035802657903180996 36-37 0.10062431221209817 38-39 0.09211781604863563 40-41 0.10127037521185482 42-43 0.10210487325320716 44-45 0.0960749519221451 46-47 0.09001811129942651 48-49 0.09114872154900065 50-51 0.09009886917439609 52-53 0.07039394768181828 54-55 0.059895423935772726 56-57 0.03308380944587177 58-59 0.014805610411089886 60-61 0.010148572954510705 62-63 0.0020997047492091114 64-65 0.005814566997809846 66-67 0.15443597623349578 68-69 0.4144763336355472 70-71 0.3896836660198858 72-73 0.18237820097297086 74-75 0.03020344523862337 76-77 0.030903346821693073 78-79 0.005033907539770561 80-81 0.0015074803327655157 82-83 5.383858331305413E-5 84-85 3.4995079153485185E-4 86-87 0.001238287416200245 88-89 2.1535433325221652E-4 90-91 6.191437081001225E-4 92-93 0.0024496555407439633 94-95 0.07892736313693736 96-97 0.01733602382680343 98-99 0.019651082909264758 100-101 0.010337007996106394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857404.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.26506823763329 #Duplication Level Percentage of deduplicated Percentage of total 1 67.66801393085852 23.863171287768456 2 14.004433272866498 9.877345899940384 3 6.336073444778245 6.70326187166283 4 3.3763868149031815 4.762740456968241 5 2.0975815957374735 3.698567905384287 6 1.3608920623372431 2.8795170865425828 7 0.9549474912359585 2.357340190925495 8 0.7131741801797241 2.012010890348492 9 0.5130984032553896 1.6285005183079797 >10 2.562002360373709 16.101867220964923 >50 0.21357465092371863 5.28017984929411 >100 0.18129453187295153 12.2108613544446 >500 0.013321253544888506 3.1694687588137858 >1k 0.004593535705133967 2.7897610341944943 >5k 1.531178568377989E-4 0.3372119937780822 >10k+ 4.593535705133967E-4 2.3281936806612604 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17679 0.951812314391484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14474 0.7792596548731455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10964 0.5902862274443256 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6245 0.33622195279002304 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4082 0.21976909708388698 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.17109901776888603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2980 0.16043897827290132 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2916 0.15699330894086586 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2375 0.12786663536850357 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2316 0.12469015895303337 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.11149970604133512 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1923 0.1035315957110031 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6919291656527066E-5 0.0 12-13 0.0 0.0 0.0 1.0767716662610826E-4 0.0 14-15 0.0 0.0 0.0 2.1535433325221652E-4 0.0 16-17 0.0 0.0 0.0 5.114665414740142E-4 0.0 18-19 1.0767716662610826E-4 0.0 0.0 7.806594580392848E-4 0.0 20-21 2.1535433325221652E-4 0.0 0.0 0.0015343996244220428 0.0 22-23 2.1535433325221652E-4 0.0 0.0 0.0039302165818529515 0.0 24-25 3.230314998783248E-4 0.0 0.0 0.007968110330332012 0.0 26-27 3.230314998783248E-4 0.0 0.0 0.014724852536120304 0.0 28-29 4.3070866650443305E-4 0.0 0.0 0.029395866488927558 0.0 30-31 5.383858331305413E-4 0.0 0.0 0.07222445951446212 0.0 32-33 5.383858331305413E-4 0.0 0.0 0.14829837773580762 0.0 34-35 6.729822914131767E-4 0.0 0.0 0.24757672536507944 0.0 36-37 8.07578749695812E-4 0.0 0.0 0.40263184530667534 0.0 38-39 8.07578749695812E-4 0.0 0.0 0.6314458243871555 0.0 40-41 8.07578749695812E-4 0.0 0.0 0.8868829829159408 0.0 42-43 8.614173330088661E-4 0.0 0.0 1.1240419424099442 0.0 44-45 9.690944996349744E-4 0.0 0.0 1.4001531169309422 0.0 46-47 0.0010498523746045557 0.0 0.0 1.7294029731819247 0.0 48-49 0.0010767716662610826 0.0 0.0 2.076957947759346 0.0 50-51 0.0010767716662610826 0.0 0.0 2.447609674578067 0.0 52-53 0.0011036909579176097 0.0 0.0 2.791234432573635 0.0 54-55 0.001184448832887191 0.0 0.0 3.14573458439844 0.0 56-57 0.001238287416200245 0.0 0.0 3.5475050123721066 0.0 58-59 0.001238287416200245 0.0 0.0 3.958670273133901 0.0 60-61 0.001238287416200245 0.0 0.0 4.393147640470248 0.0 62-63 0.001238287416200245 0.0 0.0 4.806385686689595 0.0 64-65 0.001238287416200245 0.0 0.0 5.210175061537501 0.0 66-67 0.0012921259995132993 0.0 0.0 5.642175854041447 0.0 68-69 0.0012921259995132993 0.0 0.0 6.125000269192917 0.0 70-71 0.0013728838744828803 0.0 0.0 6.676603474526813 0.0 72-73 0.0014536417494524616 0.0 0.0 7.234640390566619 0.0 74-75 0.0015343996244220428 0.0 0.0 7.795073123563856 0.0 76-77 0.001588238207735097 0.0 0.0 8.364550738557686 0.0 78-79 0.0016420767910481512 0.0 0.0 8.990181995947031 0.0 80-81 0.0020458661658960572 0.0 0.0 9.666825311025494 0.0 82-83 0.0020458661658960572 0.0 0.0 10.325917247943906 0.0 84-85 0.0021266240408656383 0.0 0.0 10.988158741986126 0.0 86-87 0.0022612204991482735 0.0 0.0 11.646712293071406 0.0 88-89 0.0023150590824613277 0.0 0.0 12.328443354272952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14675 0.0 26.452724 1 TACATGG 14645 0.0 26.146942 2 GTATCAA 20910 0.0 26.09991 1 TCGACCG 120 9.094947E-12 25.697964 18-19 GGTATCA 15390 0.0 24.884567 1 ACATGGG 14870 0.0 24.82191 3 ATGGGAG 3105 0.0 23.071825 5 GAGTACT 12020 0.0 22.833134 12-13 CATGGGA 9840 0.0 22.51585 4 AGTACTT 12020 0.0 22.28056 12-13 TATCAAC 24680 0.0 21.879322 2 CATGGGG 5025 0.0 21.714926 4 GTACTTT 12925 0.0 21.399548 14-15 ATCAACG 25425 0.0 21.216125 3 TCAACGC 25520 0.0 21.137148 4 TACACCG 180 5.2262185E-6 21.085506 5 CAACGCA 25605 0.0 21.06698 5 TAAGGTA 365 0.0 20.796663 4 AACGCAG 26090 0.0 20.711723 6 TAGCCGG 115 0.0037504027 20.627129 5 >>END_MODULE