FastQCFastQC Report
Fri 27 May 2016
SRR939851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116759
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140201.2554185818068178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98810.8847925111863884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77010.6895847716472399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50450.4517536908142222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.2283393283600132No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23440.2098930924219102No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.17855240029406524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.17344834471895906No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.15016668770970282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.13243681044880767No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14600.13073545859043895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12350.11058787079396717No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.10897606377024945No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11620.10405109786444523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG2800.038.9671259
GAGGCGC1900.034.954499
CGTATCA1201.5236401E-731.6240751
GTACATG91050.030.8425881
GATAGTC1701.09139364E-1030.695337
GCGCAGA1102.7621809E-630.1866151
TACATGG93350.029.8794122
GGAGGCG2400.029.647578
ACATGGG94800.028.5743123
GTATCAA132850.028.5652141
CATGGGG23700.028.0238444
CCGCGAC458.6015224E-426.56908290-91
CATGGGT12050.026.3776324
GGGATCG909.015601E-426.3545769
GGTATCA91950.025.7945141
TATCAAC158050.024.9711112
CATGGGA60000.024.5899224
ATCAACG161250.024.2718163
TCAACGC161200.024.1616254
CAACGCA162050.024.0056135
Produced by FastQC (version 0.11.5)