##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939851_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1116759 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.994966684844268 31.0 30.0 33.0 16.0 34.0 2 29.818694991488762 31.0 30.0 33.0 25.0 34.0 3 30.046855230179474 31.0 30.0 34.0 25.0 34.0 4 33.852610097612825 35.0 35.0 37.0 28.0 37.0 5 33.89017684209395 35.0 35.0 37.0 28.0 37.0 6 33.96375762362336 36.0 35.0 37.0 29.0 37.0 7 33.91908281016764 36.0 35.0 37.0 29.0 37.0 8 33.80194025747722 35.0 35.0 37.0 28.0 37.0 9 35.05205509872766 37.0 35.0 39.0 28.0 39.0 10-11 34.91013369939262 37.0 34.0 39.0 27.5 39.0 12-13 34.81706527549811 37.0 34.0 39.0 27.0 39.0 14-15 35.391456437781116 38.0 34.0 40.0 27.0 41.0 16-17 35.29348409101695 38.0 34.0 40.0 27.0 41.0 18-19 35.36324712852101 38.0 34.0 40.0 27.0 41.0 20-21 35.63561251800971 38.0 34.0 40.0 27.0 41.0 22-23 35.50936012156607 38.0 34.0 40.0 27.0 41.0 24-25 35.30968364705366 38.0 34.0 40.0 26.5 41.0 26-27 35.13930355609402 38.0 34.0 40.0 25.5 41.0 28-29 35.10544486321579 38.0 34.0 40.0 25.5 41.0 30-31 34.7905788088567 38.0 34.0 40.0 25.0 41.0 32-33 34.34208902726551 38.0 33.0 40.0 24.0 41.0 34-35 34.099203140516444 37.5 33.0 40.0 24.0 41.0 36-37 33.832562352306994 37.0 33.0 40.0 22.5 41.0 38-39 33.557086622986695 37.0 33.0 40.0 20.5 41.0 40-41 33.28440021526578 37.0 32.5 40.0 19.0 41.0 42-43 32.80650077590599 37.0 31.0 40.0 15.5 41.0 44-45 32.30828988170232 36.5 31.0 40.0 12.0 41.0 46-47 31.753967507761296 36.0 30.0 39.5 8.5 41.0 48-49 31.305320574985295 36.0 30.0 39.0 4.5 41.0 50-51 30.656665851808672 35.5 28.5 38.5 2.0 40.0 52-53 30.83400939683495 35.5 29.5 39.0 2.0 40.0 54-55 30.940691769665612 36.0 29.5 39.0 2.0 40.0 56-57 30.660970719734518 36.0 28.5 39.0 2.0 40.0 58-59 30.29513126825036 35.0 28.0 39.0 2.0 40.0 60-61 29.921084584946257 35.0 27.5 39.0 2.0 40.0 62-63 29.50088156889714 35.0 27.0 38.5 2.0 40.0 64-65 28.98166972462277 34.5 26.0 38.0 2.0 40.0 66-67 28.393592082087544 34.0 25.5 38.0 2.0 40.0 68-69 27.85253667084841 34.0 24.0 37.5 2.0 39.5 70-71 27.285755476338224 33.0 22.5 37.0 2.0 39.0 72-73 26.623701264104433 32.5 21.5 36.0 2.0 39.0 74-75 26.280834987674154 32.0 20.5 36.0 2.0 38.5 76-77 25.709398804934636 32.0 18.5 35.5 2.0 38.0 78-79 25.038388318339052 31.5 14.0 35.0 2.0 37.0 80-81 24.289919758873673 31.0 7.5 35.0 2.0 36.5 82-83 23.527659503975343 30.5 2.0 34.0 2.0 36.0 84-85 22.74932102629126 30.0 2.0 34.0 2.0 35.5 86-87 21.89503017213204 29.0 2.0 34.0 2.0 35.0 88-89 20.945562560946453 28.5 2.0 34.0 2.0 35.0 90-91 19.58625764377095 26.0 2.0 33.0 2.0 35.0 92-93 18.06964439059815 22.5 2.0 32.0 2.0 34.0 94-95 17.57660291969888 20.5 2.0 32.0 2.0 34.0 96-97 17.24236025856966 20.5 2.0 32.0 2.0 34.0 98-99 16.413212698532092 10.0 2.0 32.0 2.0 34.0 100-101 14.808306895220902 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3733976447350074 1101 2 0.8373752057828057 1101 3 0.6018509051553487 1101 4 0.8481623514602532 1101 5 0.792711646131977 1101 6 0.833105785096933 1101 7 0.680681914418166 1101 8 1.0043495872574795 1101 9 1.077632721711062 1101 10-11 0.9545063293189813 1101 12-13 0.8495846012202648 1101 14-15 0.987267010407777 1101 16-17 1.04765204942359 1101 18-19 0.844361043837786 1101 20-21 1.1295856914474172 1101 22-23 1.1568655201831533 1101 24-25 1.255098578750932 1101 26-27 0.6696490364797754 1101 28-29 1.0800339355689843 1101 30-31 1.2528105927672541 1101 32-33 1.1482262762835944 1101 34-35 1.1775275277695627 1101 36-37 0.9744894657728551 1101 38-39 0.9069332956032738 1101 40-41 1.147718184216039 1101 42-43 1.270624705831139 1101 44-45 1.0193254787830313 1101 46-47 1.1541904475926863 1101 48-49 1.2204086645592582 1101 50-51 0.6838568447073605 1101 52-53 0.5488434501975696 1101 54-55 0.5435273932583335 1101 56-57 0.5793841938829587 1101 58-59 0.5140254740185348 1101 60-61 0.49459983183805534 1101 62-63 -0.06738619163552784 1101 64-65 0.32007179030708244 1101 66-67 0.14669687665350395 1101 68-69 -0.05689730592783704 1101 70-71 -0.11169634330601852 1101 72-73 -0.36635399362011967 1101 74-75 -0.5053761361211393 1101 76-77 -0.8817417701678032 1101 78-79 -0.8544957389680725 1101 80-81 -0.8904738922051685 1101 82-83 -1.5589430082861995 1101 84-85 -1.1779092634743122 1101 86-87 -1.3316031797885337 1101 88-89 -5.476398830246028 1101 90-91 -8.171575239188739 1101 92-93 -9.79179227268786 1101 94-95 -7.912428922452664 1101 96-97 -4.719083209669586 1101 98-99 -3.574782932341174 1101 100-101 -2.6536563300583715 1102 1 2.001982639986668 1102 2 1.3117500079353839 1102 3 1.1158901580834915 1102 4 1.0167283533595892 1102 5 1.1632719500256172 1102 6 0.962308064299215 1102 7 1.051542946390306 1102 8 1.0044287262255054 1102 9 1.351326928738608 1102 10-11 1.3135993967453814 1102 12-13 1.2556545600680025 1102 14-15 1.3809754624495554 1102 16-17 1.5083358101073472 1102 18-19 1.4409659821093967 1102 20-21 1.408392275809561 1102 22-23 1.4466882488947732 1102 24-25 1.6091584078107672 1102 26-27 1.4524046553465055 1102 28-29 1.3740114601020608 1102 30-31 1.5466614949514863 1102 32-33 1.5942060167077798 1102 34-35 1.7180229377094278 1102 36-37 1.772265660771268 1102 38-39 1.743234340237656 1102 40-41 1.529397513117594 1102 42-43 1.6170476244762781 1102 44-45 1.5902735636200092 1102 46-47 1.5163541069785644 1102 48-49 1.4889746664585957 1102 50-51 1.6355504186231578 1102 52-53 1.596358486601499 1102 54-55 1.2510772508081907 1102 56-57 1.3114117342438334 1102 58-59 1.3285870441356593 1102 60-61 1.330774254123586 1102 62-63 1.3877261856990728 1102 64-65 1.4419616288635844 1102 66-67 1.497701941547458 1102 68-69 1.5901745522551316 1102 70-71 1.6893245493815385 1102 72-73 1.702805550539427 1102 74-75 1.1692519107291268 1102 76-77 1.1281664286292852 1102 78-79 1.3284814690091373 1102 80-81 1.0558065762975204 1102 82-83 0.6788616767407092 1102 84-85 0.7250030505491125 1102 86-87 0.8579979598126037 1102 88-89 0.4982213027074387 1102 90-91 -5.070506863120363 1102 92-93 -9.796983788990488 1102 94-95 -7.427679001591631 1102 96-97 -3.923475422395132 1102 98-99 -2.5243001846362034 1102 100-101 -2.058547118282494 1103 1 1.9662895629433805 1103 2 1.2884625150979616 1103 3 0.9058755724711567 1103 4 0.7876300567507428 1103 5 0.855223817504907 1103 6 0.7530019227145957 1103 7 0.6706527031231317 1103 8 0.5310292367219418 1103 9 0.6931147080791291 1103 10-11 0.7710107110132327 1103 12-13 0.7251120273806038 1103 14-15 1.0478804476034895 1103 16-17 0.7051009296572772 1103 18-19 0.7418293982681732 1103 20-21 0.4717797842744318 1103 22-23 0.5361579577034377 1103 24-25 0.585494554083617 1103 26-27 0.6177989877942878 1103 28-29 0.724142991070444 1103 30-31 0.6883672302226245 1103 32-33 0.7286384801135242 1103 34-35 0.6496307410120252 1103 36-37 0.7483778137118406 1103 38-39 0.7599517960901281 1103 40-41 0.5671599239109497 1103 42-43 0.560189782105077 1103 44-45 0.6471248945571304 1103 46-47 0.7806753550902563 1103 48-49 0.8394122348286572 1103 50-51 0.7188941702510157 1103 52-53 0.718317646886959 1103 54-55 0.5855592011129254 1103 56-57 0.4598450832046055 1103 58-59 0.6931231695719262 1103 60-61 0.7405427075277657 1103 62-63 0.8278913156673013 1103 64-65 0.8148948996871503 1103 66-67 0.8697900282276692 1103 68-69 0.7184029212711245 1103 70-71 0.7161842149542856 1103 72-73 0.8299621375061399 1103 74-75 0.4381324649823988 1103 76-77 0.41855034278253456 1103 78-79 0.3265006907625363 1103 80-81 -0.00981826091662441 1103 82-83 -0.27664829877630126 1103 84-85 -0.28753276233831926 1103 86-87 -0.15477747384890606 1103 88-89 -1.0898197974644646 1103 90-91 -6.504859887030349 1103 92-93 -9.5833696826197 1103 94-95 -8.386171213916473 1103 96-97 -4.980394472246672 1103 98-99 -3.702923146557339 1103 100-101 -2.9008974855273735 1104 1 1.9355506432516414 1104 2 1.377955837272527 1104 3 1.0575406699360244 1104 4 1.003301152053595 1104 5 0.8355808980921395 1104 6 0.9616499681865704 1104 7 0.7671621008983465 1104 8 0.8949620051426237 1104 9 1.070131132391296 1104 10-11 1.0704736952863456 1104 12-13 0.8869124249117206 1104 14-15 0.9032767582357053 1104 16-17 0.8872155162597792 1104 18-19 0.9732866636725035 1104 20-21 0.9528639283620208 1104 22-23 1.0065052743683651 1104 24-25 1.0437084537244488 1104 26-27 0.8039359616757906 1104 28-29 0.9238779703221027 1104 30-31 1.1582036845239827 1104 32-33 1.0447297727870932 1104 34-35 1.1355271039509631 1104 36-37 1.3399058675528934 1104 38-39 1.0839623271777583 1104 40-41 0.8201169975239466 1104 42-43 1.0683042989652805 1104 44-45 0.8506032918790254 1104 46-47 0.8601345035367451 1104 48-49 0.8051617902214758 1104 50-51 0.8480865543916138 1104 52-53 0.7132003054635199 1104 54-55 0.5764369079860288 1104 56-57 0.7308357726072643 1104 58-59 0.46260568168964866 1104 60-61 0.5097619424547126 1104 62-63 0.5036140202737762 1104 64-65 0.6102709850517272 1104 66-67 0.4176123248416772 1104 68-69 0.258211827363116 1104 70-71 0.053677676613453684 1104 72-73 0.08532008987214823 1104 74-75 0.16431023811068712 1104 76-77 0.027285566132260186 1104 78-79 -0.18222407124185835 1104 80-81 -0.021571582393765 1104 82-83 -0.33259208648073013 1104 84-85 -0.2617193927389856 1104 86-87 -0.2510776447175438 1104 88-89 -0.6339004195658013 1104 90-91 -2.8345334930560853 1104 92-93 -7.499410412124181 1104 94-95 -5.266857826881568 1104 96-97 -3.0847115207524425 1104 98-99 -2.1672813430214024 1104 100-101 -1.713944526710204 1105 1 1.9350230250044547 1105 2 1.1757208526030283 1105 3 0.8958824856276095 1105 4 0.7817419545313768 1105 5 0.7518888154786438 1105 6 0.9574430297731666 1105 7 0.9096548247470082 1105 8 0.9297443431607135 1105 9 1.138939399966894 1105 10-11 0.927090712920311 1105 12-13 0.8676636972654634 1105 14-15 0.9525647070613985 1105 16-17 0.8200118929698803 1105 18-19 1.0147000268886401 1105 20-21 0.9911676123513757 1105 22-23 0.6427194967151024 1105 24-25 0.8528024756073762 1105 26-27 0.7879961319455191 1105 28-29 0.7550448004103529 1105 30-31 1.0267567031540352 1105 32-33 1.3202819042714324 1105 34-35 1.2222689445448864 1105 36-37 1.213928839957994 1105 38-39 1.1214776965883502 1105 40-41 0.9986815334844792 1105 42-43 1.2843071627339313 1105 44-45 1.2195282891553347 1105 46-47 1.342825823516197 1105 48-49 0.9752688702669232 1105 50-51 1.2598093291344163 1105 52-53 1.0023709020301084 1105 54-55 1.3190307975752908 1105 56-57 1.280521380613365 1105 58-59 1.2086002965594567 1105 60-61 1.3862339039805995 1105 62-63 1.348143288300733 1105 64-65 1.5226793522366755 1105 66-67 1.5331085817092749 1105 68-69 1.3850409817731624 1105 70-71 1.1927509984294602 1105 72-73 1.1002474839983094 1105 74-75 1.1128767973860327 1105 76-77 0.8689246067697596 1105 78-79 0.7417739988270888 1105 80-81 0.9995883995520352 1105 82-83 0.8217175740692966 1105 84-85 1.1203089025404616 1105 86-87 1.4198309792727422 1105 88-89 0.8187119911359808 1105 90-91 -2.400779932800212 1105 92-93 -7.3879895782072005 1105 94-95 -4.956808177848869 1105 96-97 -2.3203942338619665 1105 98-99 -1.4985189891280797 1105 100-101 -0.8673460301887488 1106 1 1.5711328537542641 1106 2 1.2401338608760888 1106 3 0.8115635660353249 1106 4 0.8754495908097439 1106 5 0.8174248058645972 1106 6 0.9063922262822075 1106 7 0.815481874509949 1106 8 -0.3448971452577325 1106 9 -0.3463720672742667 1106 10-11 -0.4516345466662557 1106 12-13 0.015083952711833604 1106 14-15 0.5513770572999164 1106 16-17 0.7882212402846065 1106 18-19 1.036092561347516 1106 20-21 0.6153185059740096 1106 22-23 0.6325439597721001 1106 24-25 0.7801484640148288 1106 26-27 0.986303231830469 1106 28-29 0.7719774654502558 1106 30-31 0.6892492837779258 1106 32-33 0.5504336992108634 1106 34-35 0.6178123726263962 1106 36-37 0.5642305612532539 1106 38-39 0.4646879598754481 1106 40-41 0.6269359832782015 1106 42-43 0.4694499078664549 1106 44-45 0.385420959178127 1106 46-47 0.3011674006007645 1106 48-49 0.07207982089967402 1106 50-51 0.4244709323058835 1106 52-53 0.34057995623368953 1106 54-55 0.03845655618360411 1106 56-57 0.3238294697484676 1106 58-59 0.39131021910483454 1106 60-61 0.03160970912030692 1106 62-63 0.4522087400216641 1106 64-65 0.2705332289980902 1106 66-67 0.11090669407343867 1106 68-69 0.5293368555008762 1106 70-71 0.49193779816858907 1106 72-73 0.3277695222932806 1106 74-75 0.21037014742669768 1106 76-77 0.313805970029744 1106 78-79 0.32554516694832003 1106 80-81 -0.041928047647648015 1106 82-83 -0.1566233435151716 1106 84-85 0.12350747802025097 1106 86-87 0.2112008708948956 1106 88-89 0.49959638076863655 1106 90-91 -2.5015487999988757 1106 92-93 -7.925888850558756 1106 94-95 -5.217025849520407 1106 96-97 -2.6829388417158793 1106 98-99 -1.5964007114492258 1106 100-101 -0.8996505433677591 1107 1 1.7522299026436094 1107 2 1.1427668295533167 1107 3 0.9788319825134302 1107 4 0.9313325585701406 1107 5 0.7308590155705872 1107 6 1.0243477194114448 1107 7 0.867136396160106 1107 8 0.6590415807807659 1107 9 0.7470809925730961 1107 10-11 0.5999484618016382 1107 12-13 0.7302946232120959 1107 14-15 0.5783361517596646 1107 16-17 0.4281179862514435 1107 18-19 0.953880365232699 1107 20-21 0.7717471743252133 1107 22-23 0.7744082350868524 1107 24-25 0.6767701996214299 1107 26-27 0.7177616056573797 1107 28-29 0.7483753647963667 1107 30-31 0.8673771603498466 1107 32-33 1.0448368758630906 1107 34-35 0.812669749669567 1107 36-37 0.4289482492137324 1107 38-39 0.449714658130155 1107 40-41 0.4080579700977651 1107 42-43 0.7288607676016028 1107 44-45 0.46279317979078627 1107 46-47 0.4952257553574917 1107 48-49 0.5505314321466841 1107 50-51 0.3550100866900081 1107 52-53 0.5682820543204379 1107 54-55 0.5644172165986738 1107 56-57 0.3804692853544758 1107 58-59 0.4542294422149631 1107 60-61 0.2320354876054722 1107 62-63 0.5323612648037326 1107 64-65 0.29422655480114557 1107 66-67 0.3988283926398708 1107 68-69 0.7627600081686872 1107 70-71 0.6352598421987068 1107 72-73 0.5609364193913144 1107 74-75 0.6962394176621807 1107 76-77 0.41899773537848617 1107 78-79 0.04931174792141135 1107 80-81 0.49988836884512367 1107 82-83 0.6184621972528426 1107 84-85 0.31659398696673335 1107 86-87 0.8058022674189864 1107 88-89 0.7426724377884817 1107 90-91 -2.2899408352640442 1107 92-93 -3.8286382573660784 1107 94-95 -2.0025060146975235 1107 96-97 -0.6922048414151547 1107 98-99 -0.06157333326918035 1107 100-101 0.20493015100690393 1108 1 1.755397730062878 1108 2 1.0384418041871726 1108 3 -0.4138664889314292 1108 4 -0.011259978843945362 1108 5 0.4072685811548027 1108 6 0.6753942786333482 1108 7 0.2945860912175178 1108 8 0.6145931983330399 1108 9 0.7276647196631956 1108 10-11 0.7691955225439457 1108 12-13 0.6349700698836003 1108 14-15 0.7719570569114609 1108 16-17 0.6398296167259474 1108 18-19 0.7124142884301321 1108 20-21 0.5682989471323907 1108 22-23 0.3044955385286485 1108 24-25 0.17005228838018382 1108 26-27 0.5148152125111736 1108 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-0.08998546610763114 2311 70-71 0.07732194252501756 2311 72-73 -0.008641254831683653 2311 74-75 0.25557493140363263 2311 76-77 0.517032364064498 2311 78-79 0.3374493013833444 2311 80-81 0.35024528237854824 2311 82-83 0.6951942886797831 2311 84-85 0.9235873143170856 2311 86-87 1.2871627820053142 2311 88-89 1.6755625549822888 2311 90-91 2.505312083316113 2311 92-93 3.2585857025905156 2311 94-95 2.9521026946685964 2311 96-97 2.260700220711456 2311 98-99 2.068674009123736 2311 100-101 2.0790998248093366 2312 1 1.345986167323531 2312 2 0.6899393083469079 2312 3 -0.12781942451498196 2312 4 -0.5061111528132543 2312 5 -0.045017291596956 2312 6 0.040614942606090665 2312 7 0.16853295226920295 2312 8 0.2652164981243885 2312 9 0.32055091462925844 2312 10-11 0.05557470538735032 2312 12-13 0.44720364883931296 2312 14-15 0.2225539456947061 2312 16-17 0.09127231804385616 2312 18-19 -0.18143260695585894 2312 20-21 0.32680791366964 2312 22-23 0.5400157033333386 2312 24-25 0.5012218998742526 2312 26-27 0.6298443966751393 2312 28-29 0.5432207487557932 2312 30-31 0.6109913009479584 2312 32-33 0.5702348232921466 2312 34-35 0.603046453288492 2312 36-37 0.7775419688253606 2312 38-39 0.8283313420013201 2312 40-41 0.564568001065858 2312 42-43 0.6097577949642314 2312 44-45 0.36666980112735104 2312 46-47 0.3984822668845034 2312 48-49 0.617325636476231 2312 50-51 0.5585515944021076 2312 52-53 0.6488129251670465 2312 54-55 0.4957862455171842 2312 56-57 0.49968333918210206 2312 58-59 0.5952831052761667 2312 60-61 0.44988066211888267 2312 62-63 0.5996162503669176 2312 64-65 0.3924466376819282 2312 66-67 0.47303997799660635 2312 68-69 0.36761429358376674 2312 70-71 0.4753915687818946 2312 72-73 0.4418267145605874 2312 74-75 0.5877557320107272 2312 76-77 0.811176422750389 2312 78-79 0.708904749432417 2312 80-81 0.858701931970657 2312 82-83 0.9808311064879156 2312 84-85 0.991993056428008 2312 86-87 1.253897430712076 2312 88-89 1.474059045211849 2312 90-91 2.3025090098955125 2312 92-93 3.464465358569772 2312 94-95 3.1341522741285317 2312 96-97 2.213120697534322 2312 98-99 1.952536969978727 2312 100-101 1.9091336577316156 2313 1 -7.089928408811865 2313 2 -2.446022836763504 2313 3 -1.335142543036838 2313 4 -0.4504504447866182 2313 5 -0.384231652420155 2313 6 -0.1743317300692837 2313 7 -0.13207793351518404 2313 8 0.142220654150627 2313 9 -0.06143546562210389 2313 10-11 -0.018356244489972084 2313 12-13 -0.2307518866872016 2313 14-15 -0.23660057836518433 2313 16-17 -0.42511143840298615 2313 18-19 -0.5640774419666243 2313 20-21 -0.4505930677534167 2313 22-23 -0.797091852622799 2313 24-25 -0.29651106292179463 2313 26-27 -0.2803165867508284 2313 28-29 -0.4230215525811474 2313 30-31 -0.13258939105544698 2313 32-33 -0.36669121946986394 2313 34-35 -0.1294994883268501 2313 36-37 0.1134302568429888 2313 38-39 -0.25465774217072834 2313 40-41 -0.43922875422931895 2313 42-43 -0.06254078798104246 2313 44-45 -0.24729670029971373 2313 46-47 -1.1023188947999394 2313 48-49 -0.6038613346318762 2313 50-51 -0.6874688852934518 2313 52-53 -0.9025477978813718 2313 54-55 -0.522947167932319 2313 56-57 -0.2847102815679854 2313 58-59 -0.03523239219516583 2313 60-61 -0.24307912957907618 2313 62-63 -0.32440107475451185 2313 64-65 -0.34954925636444045 2313 66-67 -0.27163605224724563 2313 68-69 -0.33580816898696497 2313 70-71 -0.1269555845904975 2313 72-73 -0.06176975031347709 2313 74-75 -0.1235068487313491 2313 76-77 0.018543354801867196 2313 78-79 0.0073136923997196845 2313 80-81 0.19551656318591526 2313 82-83 0.14799098831656465 2313 84-85 0.20489426317779547 2313 86-87 0.8987737513770391 2313 88-89 1.396520364124271 2313 90-91 2.0277230702830735 2313 92-93 2.8874987569974913 2313 94-95 2.7955525545351207 2313 96-97 1.5476319889068115 2313 98-99 1.3256417218689691 2313 100-101 1.5024364467757323 2314 1 0.6249713474935454 2314 2 -0.06464205026288639 2314 3 -1.56772012413132 2314 4 -0.3185650759306853 2314 5 -0.29489316611738303 2314 6 -4.021323671702188 2314 7 -3.0849275156462426 2314 8 -2.7361413593946047 2314 9 -2.9997642888613 2314 10-11 -2.6756127617005845 2314 12-13 -1.5053917020972705 2314 14-15 -1.3234815617152123 2314 16-17 -2.7044077391290884 2314 18-19 -3.6483719735397777 2314 20-21 -2.9300509594214574 2314 22-23 -4.002017697704801 2314 24-25 -3.1933034142008196 2314 26-27 -2.186062471601403 2314 28-29 -1.9411958614902005 2314 30-31 -1.894060229932954 2314 32-33 -2.244944218308696 2314 34-35 -1.5567293685726398 2314 36-37 -1.9682678558944176 2314 38-39 -3.0628990047977496 2314 40-41 -2.518754048132962 2314 42-43 -1.9178732890649606 2314 44-45 -2.102530153736108 2314 46-47 -1.638256994380825 2314 48-49 -2.4734909587242484 2314 50-51 -3.227262367833653 2314 52-53 -2.111826132983861 2314 54-55 -2.4004937514737144 2314 56-57 -1.4814251389642905 2314 58-59 -0.8292786941671508 2314 60-61 -1.2398940313287952 2314 62-63 -1.0079986597361454 2314 64-65 -1.7543844012107215 2314 66-67 -1.3253896466178539 2314 68-69 -1.2148934172414805 2314 70-71 -1.150364468230066 2314 72-73 -2.088422288674195 2314 74-75 -2.2574541528911 2314 76-77 -1.317511608544276 2314 78-79 -2.3298694174223193 2314 80-81 -1.1441134595888123 2314 82-83 -1.000235996498617 2314 84-85 -2.071814962830249 2314 86-87 -0.8318826791201666 2314 88-89 0.31184396960814453 2314 90-91 1.1790323680539423 2314 92-93 2.0103222005166614 2314 94-95 2.128912674339169 2314 96-97 1.1813238871830087 2314 98-99 0.7654892757035476 2314 100-101 0.8145293547149919 2315 1 -2.8556408168034544 2315 2 -2.00422735831976 2315 3 -2.722700376895933 2315 4 -3.6538889305910303 2315 5 -3.81632681540648 2315 6 -2.9250993431081938 2315 7 -5.135792444556195 2315 8 -2.9904581050502124 2315 9 -2.4359570218786786 2315 10-11 -3.8074808501682043 2315 12-13 -2.814998732113068 2315 14-15 -1.930104784464021 2315 16-17 -2.2780530787815394 2315 18-19 -1.8310556228288775 2315 20-21 -1.2524976418859097 2315 22-23 -0.7586152490476152 2315 24-25 -2.5719527033003473 2315 26-27 -3.9560881430074026 2315 28-29 -3.1436840131489703 2315 30-31 -2.9340483815917224 2315 32-33 -3.4662929544856347 2315 34-35 -3.4163186260765883 2315 36-37 -2.630831047047657 2315 38-39 -1.5946051659351852 2315 40-41 -1.2118010465531839 2315 42-43 -2.377067601861171 2315 44-45 -5.848270675063123 2315 46-47 -2.7518351934329566 2315 48-49 -1.8545592841586895 2315 50-51 -2.6940277706772555 2315 52-53 -3.0064450113408903 2315 54-55 -1.5697097862288487 2315 56-57 -1.164622216373452 2315 58-59 -2.404756577263516 2315 60-61 -2.6667463220080982 2315 62-63 -4.564629781379118 2315 64-65 -3.765152568667279 2315 66-67 -3.3401941489875213 2315 68-69 -1.8756793572098864 2315 70-71 -3.3778822407419185 2315 72-73 -2.6477963013124253 2315 74-75 -1.3761133156083183 2315 76-77 -2.8199148470908817 2315 78-79 -1.574527790250123 2315 80-81 -1.4090956870769666 2315 82-83 -0.8801609570041471 2315 84-85 -1.919832340397388 2315 86-87 -1.7515390772244324 2315 88-89 -1.223738573835771 2315 90-91 -0.07314575200924978 2315 92-93 0.3352590093634227 2315 94-95 0.21026338523964228 2315 96-97 0.016910380074001807 2315 98-99 -0.24160985541809765 2315 100-101 -0.584040945442986 2316 1 -7.266266698824339 2316 2 -6.078519624760627 2316 3 -3.37602312597863 2316 4 -4.0219498958746875 2316 5 -2.1514121950282714 2316 6 -2.6685681548496554 2316 7 -3.2746514623377365 2316 8 -2.5218559317507463 2316 9 -7.001068509318703 2316 10-11 -6.743820816298598 2316 12-13 -5.943867275720184 2316 14-15 -4.791107465807517 2316 16-17 -5.025345627469093 2316 18-19 -4.069525492995403 2316 20-21 -2.8235613590747306 2316 22-23 -3.1298363187625426 2316 24-25 -2.9755979192190622 2316 26-27 -4.093679520371218 2316 28-29 -4.356236934402144 2316 30-31 -6.902031023420683 2316 32-33 -5.321196637804853 2316 34-35 -5.634560229237749 2316 36-37 -4.289149562430097 2316 38-39 -6.849623511969625 2316 40-41 -4.2324442161834455 2316 42-43 -4.721106551903503 2316 44-45 -3.426857677603344 2316 46-47 -4.573230903283363 2316 48-49 -5.925303356978333 2316 50-51 -5.323977671890919 2316 52-53 -6.552985725425 2316 54-55 -5.624867491563311 2316 56-57 -4.73372290787783 2316 58-59 -3.489020619036026 2316 60-61 -3.7535308266651697 2316 62-63 -4.820084904156424 2316 64-65 -3.6469042621473804 2316 66-67 -4.819065515061471 2316 68-69 -3.931154237255047 2316 70-71 -3.5754319867199023 2316 72-73 -3.0311024684024623 2316 74-75 -2.9352020116286432 2316 76-77 -3.5736521459901205 2316 78-79 -5.399130880851306 2316 80-81 -6.488873770621982 2316 82-83 -4.176740832815604 2316 84-85 -2.816404454425051 2316 86-87 -2.7845287752204797 2316 88-89 -1.8682668690658026 2316 90-91 -1.5387423509367117 2316 92-93 -1.3254480185789994 2316 94-95 -2.2220383364651255 2316 96-97 -3.540177892576292 2316 98-99 -4.983126578534101 2316 100-101 -4.078199850325854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8593.0 3 2390.0 4 2118.0 5 2170.0 6 2531.0 7 3286.0 8 4251.0 9 5190.0 10 6537.0 11 8628.0 12 11100.0 13 13325.0 14 15326.0 15 17735.0 16 20481.0 17 21956.0 18 18138.0 19 15976.0 20 16299.0 21 17669.0 22 19531.0 23 21569.0 24 24238.0 25 27183.0 26 30030.0 27 34233.0 28 38721.0 29 43965.0 30 49739.0 31 56615.0 32 64137.0 33 72830.0 34 84559.0 35 97122.0 36 107417.0 37 97919.0 38 32662.0 39 590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22235452771721 21.868185469987374 14.157006771490877 19.752453230804544 2 14.689657583802001 22.90888356343078 41.96380302260092 20.4376558301663 3 18.363509861690662 29.922013832726936 31.162547990453014 20.551928315129388 4 10.57862592818758 19.3352648063909 40.88724017248754 29.198869092933982 5 10.497757192182137 41.22929000316495 35.09162652206541 13.181326282587507 6 25.595672046120367 40.6036215138692 19.707685561560627 14.093020878449813 7 24.828658843410192 36.87604327277978 22.415934974225948 15.87936290958407 8 24.8693278230392 35.323550408787774 21.232210826149505 18.574910942023525 9 27.708030427922935 16.979954202047367 22.0260705697477 33.285944800281996 10-11 22.961394753977206 28.923171578273294 28.61921939266963 19.49621427507987 12-13 25.199001673443544 27.99834269899719 28.567187951206474 18.23546767635279 14-15 22.154429800763225 28.588974937332118 28.904175317599474 20.352419944305183 16-17 18.34931661332928 31.985572225637547 29.72244380741504 19.94266735361813 18-19 17.845705524086117 29.878113041532334 34.35271293668237 17.923468497699176 20-21 19.905961666193512 28.56826995567623 33.91528746318499 17.61048091494527 22-23 19.177480535036935 29.228512273413475 33.49032415808848 18.103683033461117 24-25 18.62646245966499 29.41015192573736 34.30307876562189 17.660306848975758 26-27 18.269655397739513 29.538702230328447 34.5620126093263 17.629629762605738 28-29 17.680302075648157 29.89409940062361 34.58706036307238 17.83853816065585 30-31 18.28075394822159 29.841218558380284 34.410498376736285 17.467529116661844 32-33 17.766479694862014 30.283374467130358 34.23046892528607 17.71967691272156 34-35 18.155003205845812 30.164474023675247 34.05622277330564 17.624299997173292 36-37 18.185477284924286 29.795599318664394 33.81101013926457 18.207913257146753 38-39 17.747710237729684 30.620405108680142 33.998404546951456 17.633480106638718 40-41 17.858012978557966 30.332118453682394 34.26135601387861 17.548512553881025 42-43 17.357358596889195 30.532026007727666 33.5680854385798 18.54252995680334 44-45 18.035925779707856 30.41080465132972 33.15185012382012 18.401419445142302 46-47 18.101445809405142 30.377522134932338 32.68979417306011 18.831237882602412 48-49 17.913875864396857 30.56039578895206 32.616006255347074 18.90972209130401 50-51 17.89572955110417 30.84142269894094 32.037748078616694 19.225099671338196 52-53 17.79943468207937 31.335912590735234 31.81975901494452 19.044893712240878 54-55 18.042239669343584 30.66162255236194 31.783261686022733 19.512876092271743 56-57 18.38551237779827 31.00106130673111 30.932222214742964 19.681204100727655 58-59 18.348324131527708 30.606313950978453 31.191809966670657 19.85355195082318 60-61 18.362577769323668 30.78090539912464 31.43262444437665 19.423892387175037 62-63 18.45826370742539 30.405374185517065 31.75416914329292 19.382192963764624 64-65 18.32642335032941 30.36082941328996 31.660926042396582 19.651821193984055 66-67 18.531730026823965 30.512113853126344 30.938273477025557 20.01788264302413 68-69 18.462268010896445 30.237902111455263 31.33768568998252 19.96214418766577 70-71 18.66925513839605 29.96755736236107 31.412827292195054 19.950360207047826 72-73 18.53411501658526 29.660804341474645 31.490860490363676 20.31422015157642 74-75 18.207596200194427 30.263936225884315 31.289242830483538 20.239224743437724 76-77 18.191069181768846 29.6365556752069 31.67442405384041 20.497951089183843 78-79 18.085890473800774 29.62478016566913 31.57898280556635 20.710346554963746 80-81 18.329829457072016 29.674173177232394 31.222702424610542 20.773294941085048 82-83 18.519083832389093 29.562521885128884 31.24361403162233 20.67478025085969 84-85 18.372926876229467 29.18842475132389 31.16203543917932 21.276612933267327 86-87 18.26654734024457 29.283161779160544 31.339926806345602 21.11036407424928 88-89 18.978407533731637 28.727132035550124 31.186923602068806 21.107536828649437 90-91 18.825067951280147 28.635371002297124 31.455461556034848 21.084099490387885 92-93 19.388941786692634 28.752408551608088 31.025567805625943 20.83308185607333 94-95 18.8878286254943 28.36406749385182 31.45406139284466 21.294042487809218 96-97 19.24690926757252 28.228824206090135 30.722693029901098 21.801573496436237 98-99 19.316724156516116 28.221542524523013 30.49921849735906 21.96251482160181 100-101 19.499820761319665 27.97704666764297 31.000385430545684 21.52274714049168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4351.0 1 3127.0 2 2123.5 3 2358.5 4 1987.0 5 1840.5 6 2968.0 7 5274.0 8 7590.0 9 8065.0 10 6481.5 11 4894.5 12 4189.5 13 3849.0 14 3584.0 15 3251.0 16 3083.5 17 2985.0 18 3006.5 19 3147.0 20 3417.0 21 3829.5 22 4308.5 23 4817.0 24 5590.5 25 7029.5 26 9308.0 27 11802.0 28 13365.5 29 15496.0 30 19075.0 31 23013.0 32 27841.5 33 33387.0 34 39136.0 35 44077.0 36 49106.5 37 53033.0 38 51763.0 39 48562.5 40 45963.0 41 43602.5 42 41599.0 43 40551.0 44 40083.0 45 38751.0 46 36862.5 47 36951.5 48 39729.5 49 37712.0 50 31245.0 51 25529.5 52 20885.0 53 17786.0 54 15324.5 55 12909.5 56 10725.5 57 8765.5 58 6864.5 59 5189.0 60 3850.5 61 2944.0 62 2258.0 63 1601.5 64 1123.0 65 875.0 66 716.5 67 582.0 68 447.0 69 325.5 70 247.0 71 201.5 72 159.0 73 118.5 74 99.0 75 75.0 76 53.0 77 40.0 78 21.5 79 15.5 80 9.5 81 3.0 82 5.0 83 6.0 84 2.5 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.5 95 1.0 96 0.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13386952780322345 2 0.12196006479464234 3 0.12831774805486235 4 0.13861540403972566 5 0.12679548586579556 6 0.14237628709506706 7 0.1681651994745509 8 0.15715118481247967 9 0.16673248212013514 10-11 0.1594793505134053 12-13 0.1515994050641186 14-15 0.1570616399778287 16-17 0.15110690847353817 18-19 0.16409090949793106 20-21 0.16964268924629217 22-23 0.20205791938994896 24-25 0.22363822454083648 26-27 0.2384578946755746 28-29 0.2329061149272135 30-31 0.22310095553293055 32-33 0.2246679901393228 34-35 0.21387783756387904 36-37 0.22180255543049124 38-39 0.2097140027526082 40-41 0.21334056855597314 42-43 0.2078335612249375 44-45 0.2004908847835567 46-47 0.21495237557969085 48-49 0.2535909717315911 50-51 0.2550236890860069 52-53 0.2572175375349561 54-55 0.25551618567658735 56-57 0.2298168181317545 58-59 0.23062272164361333 60-61 0.2010281537914626 62-63 0.19444660844461517 64-65 0.19592409821635645 66-67 0.20331154707506274 68-69 0.2029981401537843 70-71 0.2086842371541219 72-73 0.23886084643150404 74-75 0.24195014322696304 76-77 0.25739662720425804 78-79 0.25538186842461086 80-81 0.26796291769307434 82-83 0.2676495107717959 84-85 0.24262172948684543 86-87 0.24427830892788863 88-89 0.2491585024163674 90-91 0.4417246693333118 92-93 0.595652240098356 94-95 0.3427776270439728 96-97 0.28546893286734204 98-99 0.3151082731368183 100-101 0.3329276952323644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1116759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.65023965381606 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9475108106293 41.29308093095054 2 11.088576206439178 11.454552369643721 3 3.7917634496002566 5.8753647264730064 4 1.6905541435275961 3.492701066438083 5 0.9184574941446274 2.3719274842206683 6 0.5413799003347121 1.6777440957628158 7 0.3600279916629957 1.3016872436033073 8 0.24645112569903568 1.0183407764246353 9 0.17787057955893534 0.8268352255423951 >10 1.005751668601832 10.230287831090626 >50 0.13378791708615845 4.8219329061857135 >100 0.0903678263488895 8.907675538478891 >500 0.00482199837850845 1.674034512785278 >1k 0.0019645178579108503 1.6733888084878628 >5k 5.3577759761205E-4 2.087193019660155 >10k+ 1.7859253253735003E-4 1.293253464252237 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14020 1.2554185818068178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9881 0.8847925111863884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7701 0.6895847716472399 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5045 0.4517536908142222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2550 0.2283393283600132 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2344 0.2098930924219102 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1994 0.17855240029406524 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.17344834471895906 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.15016668770970282 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.13243681044880767 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1460 0.13073545859043895 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1235 0.11058787079396717 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.10897606377024945 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1162 0.10405109786444523 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7908966930197114E-4 0.0 12-13 0.0 0.0 0.0 3.134069212784495E-4 0.0 14-15 0.0 0.0 0.0 4.0295175592943507E-4 0.0 16-17 0.0 0.0 0.0 4.924965905804206E-4 0.0 18-19 0.0 0.0 0.0 7.611310945333774E-4 0.0 20-21 0.0 0.0 0.0 0.0016118070237177405 0.0 22-23 0.0 0.0 0.0 0.003357931299411959 0.0 24-25 0.0 0.0 0.0 0.007297904024055325 0.0 26-27 0.0 0.0 0.0 0.016744884079734304 0.0 28-29 0.0 0.0 0.0 0.035638844191092256 0.0 30-31 0.0 0.0 0.0 0.09048505541482092 0.0 32-33 0.0 0.0 0.0 0.18276100752266156 0.0 34-35 0.0 0.0 0.0 0.3053926585771863 0.0 36-37 0.0 0.0 0.0 0.49240704574576966 0.0 38-39 0.0 0.0 0.0 0.7522213834856043 0.0 40-41 0.0 0.0 0.0 1.0502265932040844 0.0 42-43 0.0 0.0 0.0 1.318144738479833 0.0 44-45 0.0 0.0 0.0 1.62085105201749 0.0 46-47 0.0 0.0 0.0 1.9788513009521302 0.0 48-49 0.0 0.0 0.0 2.3699383662903095 0.0 50-51 0.0 0.0 0.0 2.766845845880803 0.0 52-53 0.0 0.0 0.0 3.1288308399574127 0.0 54-55 0.0 0.0 0.0 3.5057698214207362 0.0 56-57 0.0 0.0 0.0 3.9426142972655693 0.0 58-59 0.0 0.0 0.0 4.415411024222773 0.0 60-61 0.0 0.0 0.0 4.923130236693861 0.0 62-63 0.0 0.0 0.0 5.401433970982101 0.0 64-65 0.0 0.0 0.0 5.86563439381281 0.0 66-67 0.0 0.0 0.0 6.386561469394918 0.0 68-69 0.0 0.0 0.0 6.942366258073586 0.0 70-71 0.0 0.0 0.0 7.556330416858069 0.0 72-73 0.0 0.0 0.0 8.130491896640189 0.0 74-75 0.0 0.0 0.0 8.669641346073773 0.0 76-77 0.0 0.0 0.0 9.244608729367751 0.0 78-79 0.0 0.0 0.0 9.880197965720447 0.0 80-81 0.0 0.0 0.0 10.538621134909143 0.0 82-83 0.0 0.0 0.0 11.131273623046692 0.0 84-85 0.0 0.0 0.0 11.695629943434529 0.0 86-87 0.0 0.0 0.0 12.266120084995958 0.0 88-89 0.0 0.0 0.0 12.863249814866055 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 280 0.0 38.967125 9 GAGGCGC 190 0.0 34.95449 9 CGTATCA 120 1.5236401E-7 31.624075 1 GTACATG 9105 0.0 30.842588 1 GATAGTC 170 1.09139364E-10 30.69533 7 GCGCAGA 110 2.7621809E-6 30.186615 1 TACATGG 9335 0.0 29.879412 2 GGAGGCG 240 0.0 29.64757 8 ACATGGG 9480 0.0 28.574312 3 GTATCAA 13285 0.0 28.565214 1 CATGGGG 2370 0.0 28.023844 4 CCGCGAC 45 8.6015224E-4 26.569082 90-91 CATGGGT 1205 0.0 26.377632 4 GGGATCG 90 9.015601E-4 26.354576 9 GGTATCA 9195 0.0 25.794514 1 TATCAAC 15805 0.0 24.971111 2 CATGGGA 6000 0.0 24.589922 4 ATCAACG 16125 0.0 24.271816 3 TCAACGC 16120 0.0 24.161625 4 CAACGCA 16205 0.0 24.005613 5 >>END_MODULE