FastQCFastQC Report
Fri 27 May 2016
SRR939851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116759
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143241.2826402115407174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117431.0515249933065236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92480.8281106308523146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60560.5422835186463687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.26541088990552125No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26200.2346074667855822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.19458092569659166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.1694188271596647No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.1582257228282915No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.15419620526899716No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15900.14237628709506706No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14230.12742229970835248No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.1260791271885877No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12540.11228922265233592No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12440.11139377430582605No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA300.003460535532.53677466-67
CATTCCG3200.032.384029
GGTATCA87100.030.8479271
TCGATAG504.118885E-529.28309666-67
GTACATG94050.029.2700421
GTATCAA126200.028.760781
TACATGG94650.028.3336522
ACATGGG94600.027.3011463
GGACGTG3600.026.1689056
TAGGACG3450.025.9541324
CATGGGT11450.025.9302714
CATGGGA60550.025.2953824
GTTCGAC751.1407083E-625.12327416-17
GACGTGG2251.1277734E-1025.1221497
CTGGTCG3900.024.1559129
CATGGGG21800.023.77984
CTGTAGG6150.023.7605151
GTAGGAC5450.023.3484843
GGCATTC4450.023.2873867
TATCAAC156950.023.1227952
Produced by FastQC (version 0.11.5)