FastQCFastQC Report
Fri 27 May 2016
SRR939850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199647
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149201.2436991881778556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109590.9135187267587882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86730.7229626715192052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54130.45121606605943254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.22331569203273963No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25920.21606355869685No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.17905267132748218No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.17421791577022241No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.14587624526214796No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.1372903862552901No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16060.13387271422343408No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14100.1175345747540735No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.11169952494358758No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12610.10511425444318202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3950.035.7286039
GCACCGT550.002704994834.2128456
GTACATG97600.029.1112521
TACATGG99650.028.3707562
CGGACTC505.246578E-528.26216948-49
CATGGGG25200.027.06824
GGCATTC5400.027.0059727
CGCGGAG1057.376145E-526.8815218
GGACCGC700.0087971626.8815216
ACATGGG101150.026.8350123
GTATCAA154550.026.7847391
GCATTCC5800.026.765658
CATGGGT10400.026.6876664
ACGCGGA1101.0121118E-425.6596327
GCGGAGT950.001297737824.7592969
TAGGCAT6150.024.4774825
CCGTATC1301.9226718E-924.00142194-95
AGGCTTA7500.023.834956
CATGGGA67300.023.626184
GAGTACT96350.023.50814412-13
Produced by FastQC (version 0.11.5)