##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1199647 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.238622694842732 31.0 30.0 33.0 26.0 34.0 2 30.27018031137493 31.0 30.0 33.0 26.0 34.0 3 30.17825410308199 31.0 30.0 34.0 26.0 34.0 4 33.85532994289154 35.0 35.0 37.0 28.0 37.0 5 33.81373770784239 35.0 35.0 37.0 28.0 37.0 6 33.83584921230995 35.0 35.0 37.0 28.0 37.0 7 33.857573102754394 35.0 35.0 37.0 28.0 37.0 8 33.7327463828943 35.0 35.0 37.0 28.0 37.0 9 35.00109365505019 37.0 34.0 39.0 28.0 39.0 10-11 34.829365221602686 37.0 34.0 39.0 27.0 39.0 12-13 34.75679512389895 37.0 34.0 39.0 27.0 39.0 14-15 35.266016586545874 38.0 33.5 40.0 27.0 41.0 16-17 35.15961737077657 38.0 33.5 40.0 27.0 41.0 18-19 35.385121206488236 38.0 34.0 40.0 27.0 41.0 20-21 35.59150525112804 38.0 34.0 40.0 27.0 41.0 22-23 35.49304628778299 38.0 34.0 40.0 27.0 41.0 24-25 35.28858614242356 38.0 34.0 40.0 26.5 41.0 26-27 35.11840107965093 38.0 34.0 40.0 25.5 41.0 28-29 34.967412913965525 38.0 34.0 40.0 25.0 41.0 30-31 34.69128752041225 38.0 33.5 40.0 24.5 41.0 32-33 34.28204880268946 38.0 33.0 40.0 24.0 41.0 34-35 33.96667644732159 37.0 33.0 40.0 23.5 41.0 36-37 33.68977165782935 37.0 33.0 40.0 21.5 41.0 38-39 33.359244010946554 37.0 32.0 40.0 19.0 41.0 40-41 33.03033225607199 37.0 31.5 40.0 17.5 41.0 42-43 32.63101854128756 37.0 31.0 40.0 15.5 41.0 44-45 32.07977263311624 36.0 30.5 39.5 9.5 41.0 46-47 31.52865217851585 36.0 30.0 39.0 8.5 40.5 48-49 31.10236136130045 36.0 30.0 39.0 2.0 40.0 50-51 30.351653027932386 35.0 28.5 38.5 2.0 40.0 52-53 30.479773633410495 35.0 28.5 38.5 2.0 40.0 54-55 30.576980144992653 36.0 28.5 39.0 2.0 40.0 56-57 30.387570260251557 35.0 28.0 39.0 2.0 40.0 58-59 29.89363037626902 35.0 27.0 39.0 2.0 40.0 60-61 29.44544686895395 35.0 26.5 38.5 2.0 40.0 62-63 29.029317374194243 34.5 26.0 38.0 2.0 40.0 64-65 28.528996446454666 34.0 25.5 38.0 2.0 40.0 66-67 28.012998407031404 34.0 24.0 38.0 2.0 39.5 68-69 27.466745634340768 33.0 22.5 37.0 2.0 39.0 70-71 26.968565336303094 33.0 22.0 36.5 2.0 39.0 72-73 26.491023192655838 32.5 20.5 36.0 2.0 39.0 74-75 25.905827714319294 32.0 19.0 36.0 2.0 38.0 76-77 25.24487911860739 31.5 15.5 35.0 2.0 37.5 78-79 24.596356261466916 31.0 10.0 35.0 2.0 37.0 80-81 23.889608359792504 31.0 4.5 34.5 2.0 36.0 82-83 23.13399816779436 30.0 2.0 34.0 2.0 36.0 84-85 22.375827639297228 29.0 2.0 34.0 2.0 35.0 86-87 21.61769712257022 29.0 2.0 34.0 2.0 35.0 88-89 20.788081410614957 28.5 2.0 33.5 2.0 35.0 90-91 19.899620054899483 26.5 2.0 33.0 2.0 35.0 92-93 18.958927501173264 25.5 2.0 32.5 2.0 34.0 94-95 17.6365801773355 22.5 2.0 32.0 2.0 34.0 96-97 15.062408775248052 2.0 2.0 31.0 2.0 34.0 98-99 13.735518865132827 2.0 2.0 30.5 2.0 34.0 100-101 12.48059929295868 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49175432011809406 1101 2 0.34809199622036147 1101 3 0.5673539745617759 1101 4 0.6346459361976358 1101 5 0.48212004362027727 1101 6 0.5167105324542618 1101 7 0.4392599281575045 1101 8 0.188438045170102 1101 9 0.4191642017868773 1101 10-11 0.44967108947431456 1101 12-13 0.36803503001630133 1101 14-15 0.5406148610055794 1101 16-17 0.6964653821070854 1101 18-19 0.35746940822338047 1101 20-21 0.46601615055655543 1101 22-23 0.5192530137328291 1101 24-25 0.6608844907708331 1101 26-27 0.5089051390885899 1101 28-29 0.962382959123012 1101 30-31 0.8583356376068494 1101 32-33 0.6300908680833786 1101 34-35 0.3352382843076711 1101 36-37 0.44465209236475545 1101 38-39 0.3398491819180123 1101 40-41 0.21358193827923344 1101 42-43 0.5193171829181367 1101 44-45 0.3879348340052431 1101 46-47 0.18385361937796674 1101 48-49 0.06432835816763927 1101 50-51 0.3155727614243524 1101 52-53 0.4152508259503591 1101 54-55 0.27821718834474396 1101 56-57 0.3615898782431124 1101 58-59 0.04702833678772933 1101 60-61 0.30390608420442433 1101 62-63 0.015019180146090605 1101 64-65 0.4089376940710494 1101 66-67 0.3345527436184881 1101 68-69 0.4557742632046846 1101 70-71 0.1981458951286541 1101 72-73 -0.04389577124866406 1101 74-75 -0.047096049981757204 1101 76-77 -0.5532867144811462 1101 78-79 -0.06171300478945341 1101 80-81 -0.4856878336182717 1101 82-83 -0.6084616977576225 1101 84-85 -0.5589660825612626 1101 86-87 -0.4411728024564141 1101 88-89 -0.3123583268943051 1101 90-91 -0.43273518535532673 1101 92-93 -0.7956805428390368 1101 94-95 -0.7824242100108805 1101 96-97 0.7725519888978365 1101 98-99 0.8038637328796323 1101 100-101 0.2460552311524733 1102 1 0.9461248372224844 1102 2 0.8191072303558542 1102 3 0.8410060673960373 1102 4 1.1018611623514403 1102 5 1.1514946785488576 1102 6 1.0870455092892328 1102 7 0.8844333665588451 1102 8 0.8068960560614755 1102 9 1.1926969321401444 1102 10-11 1.154926666677163 1102 12-13 0.8690719235442472 1102 14-15 0.933609117819941 1102 16-17 1.0405681862183869 1102 18-19 1.0329210083699536 1102 20-21 0.9670312758508786 1102 22-23 0.7751545632682522 1102 24-25 0.7582033699970481 1102 26-27 1.1389282496966118 1102 28-29 1.2665178350985045 1102 30-31 0.9946831456883132 1102 32-33 1.3759819325862779 1102 34-35 1.02709442530778 1102 36-37 0.9091136369660617 1102 38-39 0.9267592174365191 1102 40-41 1.0984200007613154 1102 42-43 1.237939906777207 1102 44-45 1.077922172149357 1102 46-47 1.175277298136887 1102 48-49 0.9738748170202349 1102 50-51 0.753984623660461 1102 52-53 0.8697425195552135 1102 54-55 0.6955722753995381 1102 56-57 0.8057311395671292 1102 58-59 1.0555513258564488 1102 60-61 1.0603554676192068 1102 62-63 0.9108944626796251 1102 64-65 0.7382624071838713 1102 66-67 0.7628862764939619 1102 68-69 0.631322006383062 1102 70-71 1.0865474174537901 1102 72-73 0.8437913902099545 1102 74-75 0.8788233857856298 1102 76-77 0.8806583062168158 1102 78-79 0.8824220888683705 1102 80-81 0.3959746063830316 1102 82-83 0.28265359769172704 1102 84-85 0.3197391429032166 1102 86-87 0.18733487628799494 1102 88-89 0.0765046819835078 1102 90-91 0.052004194467698994 1102 92-93 -0.04601196419584852 1102 94-95 0.22779561286825967 1102 96-97 1.6003736054794242 1102 98-99 1.5810186973067122 1102 100-101 1.213998604720759 1103 1 0.7474802666649651 1103 2 0.8570091299483309 1103 3 0.8658350079432218 1103 4 0.7525874569273228 1103 5 1.037552775248514 1103 6 0.8621465313544476 1103 7 0.8180976608806674 1103 8 0.7723422182529589 1103 9 0.7673406512627494 1103 10-11 0.7073469047356227 1103 12-13 0.548414327405915 1103 14-15 0.939796783686667 1103 16-17 1.0807497341133114 1103 18-19 0.8267374623495556 1103 20-21 0.8464952151792815 1103 22-23 0.8449080247974692 1103 24-25 0.9794106222998664 1103 26-27 1.156368245851425 1103 28-29 1.0626266402400688 1103 30-31 1.0269405124885296 1103 32-33 1.232322547747998 1103 34-35 1.1460996197473605 1103 36-37 1.1278585944458115 1103 38-39 1.1465743050004136 1103 40-41 1.1851843077860025 1103 42-43 1.236732791802602 1103 44-45 1.26662074702584 1103 46-47 1.2432605990264314 1103 48-49 1.1533744616834873 1103 50-51 1.3168373425873767 1103 52-53 1.4593663432532544 1103 54-55 1.299533164325048 1103 56-57 1.1040001174675886 1103 58-59 0.917491498821331 1103 60-61 1.2361091420071517 1103 62-63 1.0083768932516257 1103 64-65 0.8985628813095197 1103 66-67 1.1695614575097117 1103 68-69 0.8232048791519091 1103 70-71 0.9969802416427811 1103 72-73 1.0067371489998145 1103 74-75 0.8643307963592122 1103 76-77 0.8654883453896893 1103 78-79 0.6908502644974206 1103 80-81 0.4161103553195993 1103 82-83 0.5981411300067698 1103 84-85 0.2691845085910778 1103 86-87 0.1677326474511318 1103 88-89 0.15013800879374983 1103 90-91 -0.07044999799092366 1103 92-93 0.16410122858206222 1103 94-95 0.4114988740699488 1103 96-97 1.8298390897501928 1103 98-99 1.7455121450344446 1103 100-101 1.244052540375625 1104 1 0.6198577013434985 1104 2 0.6398471151537244 1104 3 0.7109852329684578 1104 4 0.893012658403407 1104 5 1.0423152748686917 1104 6 1.2191462848901935 1104 7 0.8688443125118894 1104 8 0.8777121003215029 1104 9 1.0416735940796684 1104 10-11 0.8298133028800478 1104 12-13 1.000573928201355 1104 14-15 1.1203328603134182 1104 16-17 1.1236625364118495 1104 18-19 1.2262067201545364 1104 20-21 1.1278993186402388 1104 22-23 0.9462699930002074 1104 24-25 1.114055393037475 1104 26-27 1.389337059683129 1104 28-29 1.2255965682252423 1104 30-31 1.2042057046462418 1104 32-33 1.3235649639079483 1104 34-35 1.1616690703346535 1104 36-37 1.0273096206486727 1104 38-39 0.7252325266089201 1104 40-41 0.9870783686727975 1104 42-43 0.8561656539277962 1104 44-45 0.953218493734866 1104 46-47 1.0665075798393566 1104 48-49 0.9177846862360575 1104 50-51 1.2744093658563038 1104 52-53 1.2417333454039117 1104 54-55 1.2407129515554978 1104 56-57 1.242574811328602 1104 58-59 0.9038819454618228 1104 60-61 0.9514159224750287 1104 62-63 0.7388861289085931 1104 64-65 0.6872285643699954 1104 66-67 0.6203000062066586 1104 68-69 0.46810965279306416 1104 70-71 0.8266160485093437 1104 72-73 1.086137801961268 1104 74-75 0.7791963045313608 1104 76-77 0.5606870192094426 1104 78-79 0.48199139397893376 1104 80-81 0.5345060418575862 1104 82-83 0.5778004016205038 1104 84-85 0.034099140802268835 1104 86-87 0.34343025944887273 1104 88-89 0.3644697361705269 1104 90-91 0.2442726959646251 1104 92-93 0.3243121074465698 1104 94-95 0.4370643069523297 1104 96-97 1.979367600262064 1104 98-99 2.1080509293917284 1104 100-101 1.8826279094811635 1105 1 0.5446688342777577 1105 2 0.8745818968589241 1105 3 0.7361684021812813 1105 4 0.7786741791268312 1105 5 0.7727894032647811 1105 6 0.8448248321387979 1105 7 0.802704976546373 1105 8 0.7703317049575489 1105 9 0.9095343519611419 1105 10-11 0.9689870471738189 1105 12-13 0.4488528414532169 1105 14-15 0.7022525675108113 1105 16-17 0.93266514401747 1105 18-19 0.9983770658466256 1105 20-21 0.8229415162685925 1105 22-23 0.7744391723082842 1105 24-25 0.8594347694912585 1105 26-27 1.2221818300596539 1105 28-29 1.2525866271477426 1105 30-31 1.1520602954398456 1105 32-33 1.157722251461827 1105 34-35 1.2452733309879633 1105 36-37 1.1681437863154258 1105 38-39 1.1396226217295435 1105 40-41 1.02486049618404 1105 42-43 1.2992075700151773 1105 44-45 1.3595578578544831 1105 46-47 1.1778791535863355 1105 48-49 1.29464637588406 1105 50-51 1.3542845493606812 1105 52-53 1.2930944619149471 1105 54-55 1.168084766623302 1105 56-57 0.8549646249082592 1105 58-59 0.7325357774354906 1105 60-61 0.738549165089605 1105 62-63 0.7179093158182788 1105 64-65 0.9222375133507512 1105 66-67 0.9215384512961116 1105 68-69 0.5255644554127379 1105 70-71 0.8568213723149718 1105 72-73 0.9131352345757797 1105 74-75 0.839619688519722 1105 76-77 0.8040845483522894 1105 78-79 0.5607538203258073 1105 80-81 0.6982690379897498 1105 82-83 0.5614924762422682 1105 84-85 0.2875946059816812 1105 86-87 0.2661124063998521 1105 88-89 0.559234414845136 1105 90-91 0.5546789863942436 1105 92-93 0.41423629248601657 1105 94-95 0.6682215073625777 1105 96-97 1.939285216012271 1105 98-99 2.029985316939575 1105 100-101 1.484818901510872 1106 1 0.8668251553466639 1106 2 0.9735183298493766 1106 3 1.0726286490219543 1106 4 0.9763837906948964 1106 5 0.9361772588399901 1106 6 1.0784546733419447 1106 7 1.0327148285952887 1106 8 0.8412252886332396 1106 9 1.145561903627609 1106 10-11 1.1444827017672239 1106 12-13 1.1691327304970045 1106 14-15 1.389294567902624 1106 16-17 1.3791205358138683 1106 18-19 1.126671610877871 1106 20-21 1.1549811175303404 1106 22-23 1.2388135104181188 1106 24-25 1.2786919761088882 1106 26-27 1.297851670013543 1106 28-29 1.4895440191655993 1106 30-31 1.2516774283516554 1106 32-33 1.3321390073269228 1106 34-35 1.323485680971352 1106 36-37 1.5444814077980027 1106 38-39 1.6628956029822604 1106 40-41 1.5419759468655911 1106 42-43 1.4264629652186258 1106 44-45 1.6860106091882017 1106 46-47 1.5733956195683696 1106 48-49 1.2665446130203257 1106 50-51 1.4504804376208433 1106 52-53 1.6134727294875084 1106 54-55 1.442055483040452 1106 56-57 1.4880403948302678 1106 58-59 1.3450194874842971 1106 60-61 1.6218357461447148 1106 62-63 1.6573220542052098 1106 64-65 1.6989303196515202 1106 66-67 1.3850248273604606 1106 68-69 1.4208005496857687 1106 70-71 1.4628089763364258 1106 72-73 1.5061029442725236 1106 74-75 1.3904393676325242 1106 76-77 1.5898223589260603 1106 78-79 1.273783793836003 1106 80-81 1.4483371789620634 1106 82-83 1.2285613639260724 1106 84-85 1.1683209319624126 1106 86-87 1.5604033286027388 1106 88-89 1.8314519208287692 1106 90-91 1.7509441513095894 1106 92-93 1.969440417286208 1106 94-95 2.1178688778308477 1106 96-97 3.794252125052589 1106 98-99 3.9337880090758244 1106 100-101 3.3060759037055814 1107 1 0.47999331514776955 1107 2 0.5219373219479095 1107 3 0.6348930695571973 1107 4 0.3024786368806218 1107 5 0.7677445499083291 1107 6 0.654247320547853 1107 7 0.5825912342134743 1107 8 0.4914489015899335 1107 9 0.6634595131836747 1107 10-11 0.7788383678194961 1107 12-13 0.6664579480373973 1107 14-15 1.008714603052006 1107 16-17 0.823628801634662 1107 18-19 0.8753363629032478 1107 20-21 0.7973389839912457 1107 22-23 0.7803876599030204 1107 24-25 0.6247105224103393 1107 26-27 0.9195549863437265 1107 28-29 0.8760115059845788 1107 30-31 0.7503278920091887 1107 32-33 0.7900140412617915 1107 34-35 0.6817615036241307 1107 36-37 0.4987143367705258 1107 38-39 0.6278655259679553 1107 40-41 0.5687452040133465 1107 42-43 0.3787240994292631 1107 44-45 0.29928164523775536 1107 46-47 0.294665998353004 1107 48-49 0.4084688275173072 1107 50-51 0.5959574547811748 1107 52-53 0.6321897769391818 1107 54-55 0.24314354225144896 1107 56-57 0.3658062656348484 1107 58-59 0.256292295291459 1107 60-61 0.4107722629263968 1107 62-63 0.35227040175546875 1107 64-65 0.3596115890301199 1107 66-67 0.36007162176513674 1107 68-69 0.15631428795144942 1107 70-71 0.3425523572500211 1107 72-73 0.39902448684471636 1107 74-75 0.3726332036306417 1107 76-77 0.4962907030471122 1107 78-79 0.3763914209667547 1107 80-81 0.22643668315570054 1107 82-83 0.34431357505099314 1107 84-85 0.47151438217459685 1107 86-87 0.34269146782859394 1107 88-89 0.5046512292717651 1107 90-91 0.6504090519339911 1107 92-93 0.5355255473817984 1107 94-95 0.48501491923853735 1107 96-97 1.9262252057675777 1107 98-99 2.2897919812420806 1107 100-101 2.006434541738356 1108 1 0.6410655581998341 1108 2 0.6014111214402611 1108 3 0.5180273383008362 1108 4 0.7471064698467487 1108 5 0.6417398857412451 1108 6 0.5906497273611464 1108 7 0.5199947619729954 1108 8 0.5833883962622153 1108 9 0.8683427157399422 1108 10-11 0.6267910303889082 1108 12-13 0.4611643760842483 1108 14-15 0.6191741910076374 1108 16-17 0.7819869375139561 1108 18-19 0.8070301513149829 1108 20-21 0.633142264369539 1108 22-23 0.6461111856010362 1108 24-25 0.8203162843316676 1108 26-27 0.8990384491656727 1108 28-29 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0.5276870688035977 2311 78-79 0.5682257098275727 2311 80-81 0.5190872192228149 2311 82-83 0.8360652674683173 2311 84-85 0.997877223492253 2311 86-87 1.1264442615318302 2311 88-89 1.1364589577076138 2311 90-91 1.381002126789042 2311 92-93 1.4594396470805648 2311 94-95 1.9202981764535778 2311 96-97 3.4122518309740695 2311 98-99 3.3615035091143337 2311 100-101 2.6555875233080126 2312 1 -0.12134271095134608 2312 2 -0.653926175540569 2312 3 -0.6871759584579245 2312 4 -0.4139045126196663 2312 5 -2.0407363677033956 2312 6 -0.6635961365491383 2312 7 0.0034731150068054717 2312 8 0.1796136781421538 2312 9 0.17347711972916358 2312 10-11 0.0017191093657586975 2312 12-13 0.22534795891203174 2312 14-15 0.17965409349786654 2312 16-17 -0.314143711440785 2312 18-19 -0.14064351902933225 2312 20-21 0.19882129976982554 2312 22-23 0.2937764020706979 2312 24-25 0.34159986009353105 2312 26-27 0.45249217240348116 2312 28-29 0.4316643722266136 2312 30-31 0.35233452035573265 2312 32-33 -0.5061850162701802 2312 34-35 -0.10443289335168515 2312 36-37 0.16378152645516053 2312 38-39 0.17856590399084382 2312 40-41 0.5757554572368946 2312 42-43 0.2908978604980845 2312 44-45 0.4327580317532096 2312 46-47 0.16139221657060077 2312 48-49 0.28528838631799047 2312 50-51 0.05336514987723362 2312 52-53 -0.12903633771050238 2312 54-55 -0.035816635885364434 2312 56-57 -0.276905789670419 2312 58-59 0.12137243096542605 2312 60-61 0.1800281274595683 2312 62-63 0.09890509182848461 2312 64-65 -0.1850935013328865 2312 66-67 -0.03518173124610158 2312 68-69 -0.08876688340166439 2312 70-71 0.03953594173555075 2312 72-73 -0.029598448269982924 2312 74-75 0.13361658098033757 2312 76-77 0.17515896465966918 2312 78-79 0.35432304025110284 2312 80-81 -0.011089208822962604 2312 82-83 0.2469364324897576 2312 84-85 0.3272060863846349 2312 86-87 0.3790243868902685 2312 88-89 0.1962240375157691 2312 90-91 0.4914361867276327 2312 92-93 0.4363955178649448 2312 94-95 0.9560592721979901 2312 96-97 2.3350006446366205 2312 98-99 2.55803870590678 2312 100-101 2.3071148534777794 2313 1 0.01948228146600428 2313 2 -0.3836956629533681 2313 3 -0.5411619052782655 2313 4 -0.13919770845426171 2313 5 -0.3797389963557052 2313 6 0.23421247734227535 2313 7 0.2650341300710011 2313 8 0.32038811955354163 2313 9 0.23163605209192184 2313 10-11 -0.7006138195595284 2313 12-13 0.017067704134966277 2313 14-15 0.03807968241708437 2313 16-17 -0.17125118287946606 2313 18-19 0.17441688942279399 2313 20-21 -1.9247961302170324 2313 22-23 -0.16553762077835898 2313 24-25 0.23400204389699297 2313 26-27 0.2093923847177095 2313 28-29 -0.039464684491612445 2313 30-31 0.4886554181415974 2313 32-33 0.61428814114565 2313 34-35 0.29474059770078753 2313 36-37 -0.26680056837852106 2313 38-39 0.26691895221649986 2313 40-41 0.4611184134597295 2313 42-43 0.4820729186317365 2313 44-45 0.15738269053392173 2313 46-47 0.3349970088059635 2313 48-49 0.14642856425752981 2313 50-51 0.4258451822298781 2313 52-53 0.45877707852648086 2313 54-55 0.33117102966917855 2313 56-57 0.5112766489101084 2313 58-59 0.15834417295309322 2313 60-61 0.15508120601583286 2313 62-63 0.10255108313370798 2313 64-65 0.20712417129880123 2313 66-67 0.21412698374732742 2313 68-69 0.2588888136967036 2313 70-71 0.37647608934448584 2313 72-73 0.33484523790937004 2313 74-75 0.7247044386287058 2313 76-77 0.702785089419546 2313 78-79 0.3620282773353196 2313 80-81 0.7468586730898856 2313 82-83 0.8608834550355091 2313 84-85 0.7250083814003041 2313 86-87 0.7051769498262708 2313 88-89 0.7867166570688973 2313 90-91 1.0183242668004269 2313 92-93 1.2084736697201635 2313 94-95 1.4834566536558818 2313 96-97 3.196666553038197 2313 98-99 3.407726553444947 2313 100-101 2.890580883201773 2314 1 -0.06909996961624643 2314 2 -0.026444580702285947 2314 3 -1.0766777782941404 2314 4 -0.20512483688138872 2314 5 -0.31276569188239733 2314 6 -0.12336328023347676 2314 7 -0.24766428262741158 2314 8 -2.010528186362766 2314 9 -1.8002903548344875 2314 10-11 -0.48518272287842734 2314 12-13 -0.3573641140468524 2314 14-15 -0.5607659164285081 2314 16-17 -0.5956108509891394 2314 18-19 -1.5768096618037504 2314 20-21 -1.0905068084277119 2314 22-23 -1.895416769407511 2314 24-25 -1.608124579479636 2314 26-27 -1.6568143252390044 2314 28-29 -2.7674138186907484 2314 30-31 -1.5093424173562369 2314 32-33 -1.1379034172959095 2314 34-35 -1.736130614420432 2314 36-37 -3.5284257487537047 2314 38-39 -3.4869771516796 2314 40-41 -4.144897098805785 2314 42-43 -3.077432853374031 2314 44-45 -3.283282893881662 2314 46-47 -3.2825625150035584 2314 48-49 -1.4701659523369877 2314 50-51 -1.0326324512477534 2314 52-53 -2.308313469814067 2314 54-55 -1.9046695186713691 2314 56-57 -2.0793889355054738 2314 58-59 -3.910288583179664 2314 60-61 -2.1965151143304915 2314 62-63 -2.3836953093626825 2314 64-65 -1.7448870119623834 2314 66-67 -2.674608586238243 2314 68-69 -2.6734073623494687 2314 70-71 -2.2013467822283843 2314 72-73 -1.6742366524225183 2314 74-75 -2.1022796883554236 2314 76-77 -1.1542675801331193 2314 78-79 -0.8122772782202361 2314 80-81 -0.6142434192622055 2314 82-83 -0.5358878292357758 2314 84-85 -0.6864995551076305 2314 86-87 -0.24371374281442115 2314 88-89 0.13565244350468575 2314 90-91 0.5009541369302966 2314 92-93 1.0269711444676375 2314 94-95 1.5411857301349592 2314 96-97 3.0592747781464222 2314 98-99 3.032762628481022 2314 100-101 2.704839541210431 2315 1 -2.3747705920972138 2315 2 -1.8100503427563233 2315 3 -1.3479216206586706 2315 4 -1.5783055354931221 2315 5 -1.8282064293058973 2315 6 -3.2832752073867866 2315 7 -2.136416335172566 2315 8 -1.45057520830634 2315 9 -1.0680355406831197 2315 10-11 -3.562586999636153 2315 12-13 -1.9627763024331841 2315 14-15 -0.8963757089348547 2315 16-17 -1.7605095298964315 2315 18-19 -1.2480105873150222 2315 20-21 -0.9962110180112305 2315 22-23 -1.086569366810103 2315 24-25 -2.9006449183361838 2315 26-27 -3.7019376645433795 2315 28-29 -5.731377656576999 2315 30-31 -8.246402053122772 2315 32-33 -6.367485257869127 2315 34-35 -5.936006528735994 2315 36-37 -3.3263106942679634 2315 38-39 -6.182957895074139 2315 40-41 -3.5135668629496877 2315 42-43 -3.207144377482109 2315 44-45 -2.477551628021086 2315 46-47 -1.8248962588459214 2315 48-49 -2.3633962852224926 2315 50-51 -1.9454739201237068 2315 52-53 -1.054017119612542 2315 54-55 -2.198864547780193 2315 56-57 -2.0788711343411244 2315 58-59 -1.1732102879117114 2315 60-61 -1.665936856381542 2315 62-63 -2.1820000936588464 2315 64-65 -1.6268796601188242 2315 66-67 -2.398256705360499 2315 68-69 -1.4784948754111191 2315 70-71 -1.9571172330419415 2315 72-73 -1.300444855139343 2315 74-75 -1.910982479816834 2315 76-77 -1.3219138706242397 2315 78-79 -1.2133959153302527 2315 80-81 -0.988417736324454 2315 82-83 -0.8708352094417755 2315 84-85 -0.9664667143851347 2315 86-87 -1.5442483375300498 2315 88-89 -1.929553184200941 2315 90-91 -1.5665279499483447 2315 92-93 -0.731113084521759 2315 94-95 -0.2573616276796429 2315 96-97 1.1277831376030587 2315 98-99 1.1152936032533756 2315 100-101 0.8811391754842024 2316 1 -3.1058771642466816 2316 2 -2.1559993194874707 2316 3 -2.1372403759151872 2316 4 -2.8551888708289397 2316 5 -2.213444124047374 2316 6 -5.629324308271883 2316 7 -3.191224297045288 2316 8 -4.1629385388361975 2316 9 -6.032531515692135 2316 10-11 -5.462442820854463 2316 12-13 -3.4181509670172687 2316 14-15 -4.857307264063216 2316 16-17 -4.119544733548963 2316 18-19 -2.637153124060589 2316 20-21 -3.398259558201069 2316 22-23 -4.136671493447135 2316 24-25 -2.8757240164178057 2316 26-27 -3.9527887198519593 2316 28-29 -3.4861836159666417 2316 30-31 -3.514824897449735 2316 32-33 -3.624213601014631 2316 34-35 -5.550758821579123 2316 36-37 -5.661966907972957 2316 38-39 -5.361859733353604 2316 40-41 -4.952070607593043 2316 42-43 -4.028172452294875 2316 44-45 -3.718590516242479 2316 46-47 -3.982681913057146 2316 48-49 -4.395946647671931 2316 50-51 -5.216139545499171 2316 52-53 -3.5952282213787363 2316 54-55 -3.681232684269439 2316 56-57 -3.130114676333193 2316 58-59 -3.7894043685717307 2316 60-61 -4.110337913692607 2316 62-63 -3.054752306402648 2316 64-65 -3.4841927283234 2316 66-67 -4.2237102677779 2316 68-69 -3.1166461830325716 2316 70-71 -2.719292330162368 2316 72-73 -3.1282813724207728 2316 74-75 -4.0149614136948415 2316 76-77 -4.039874141359949 2316 78-79 -3.9745197088341975 2316 80-81 -4.112952159008582 2316 82-83 -3.0092468398635006 2316 84-85 -2.8255419676992446 2316 86-87 -2.476484310136083 2316 88-89 -2.9735377250315693 2316 90-91 -2.4953997509816226 2316 92-93 -2.24765359475445 2316 94-95 -2.006911068986863 2316 96-97 -0.13416167396328582 2316 98-99 0.2942354911944225 2316 100-101 -0.08075357152208618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9647.0 3 2479.0 4 2207.0 5 2491.0 6 2914.0 7 3713.0 8 4690.0 9 6009.0 10 7413.0 11 9610.0 12 12477.0 13 14943.0 14 17179.0 15 19957.0 16 22436.0 17 23774.0 18 19945.0 19 17420.0 20 18227.0 21 19929.0 22 21852.0 23 24285.0 24 26802.0 25 30016.0 26 33901.0 27 37853.0 28 42530.0 29 48346.0 30 54958.0 31 61700.0 32 71721.0 33 82891.0 34 95960.0 35 107241.0 36 108563.0 37 89998.0 38 23339.0 39 231.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22883687081461 21.165883279809318 14.526512748953444 20.078767100422628 2 14.703469570841419 22.916358932632324 41.961569756525 20.41860174000125 3 18.371184458823915 29.93150324789949 31.136435147588926 20.560877145687677 4 10.710192579459582 19.30736169228856 40.844024076814755 29.138421651437103 5 10.589705364342224 41.19724011746028 35.01939029500293 13.193664223194565 6 25.596670514672926 40.58060654928995 19.72324404456342 14.099478891473701 7 24.817999610997905 36.849099743976524 22.47238377310326 15.860516871922314 8 24.916670631750556 35.25401996899722 21.26653961703133 18.562769782220897 9 27.716988865986952 16.94103072059279 22.01273770495909 33.329242708461166 10-11 23.010843078803276 28.99273482832975 28.548292981007705 19.44812911185927 12-13 25.255851324700263 27.95262592217712 28.549038431438024 18.242484321684596 14-15 22.21086132912433 28.65816352507831 28.853561612163592 20.277413533633762 16-17 18.381799332566516 31.85095933129776 29.78931104771031 19.977930288425423 18-19 17.856870038616197 29.904446727307715 34.261427175432566 17.97725605864352 20-21 19.938058673476846 28.560154364767353 33.93477746177522 17.567009499980582 22-23 19.228004849505382 29.187783021803142 33.48167507562213 18.10253705306934 24-25 18.68257044753924 29.38286869564491 34.258682194504544 17.6758786623113 26-27 18.31306086252579 29.49649723952148 34.56423203475066 17.62620986320207 28-29 17.73535299189285 29.847038517320428 34.57715661571571 17.84045187507101 30-31 18.256864484802307 29.8605715488764 34.38224231760847 17.50032164871282 32-33 17.701351568684377 30.270835900638502 34.285899280876066 17.741913249801055 34-35 18.20979018642764 30.127302992313542 34.085455537078815 17.57745128418 36-37 18.27202641654241 29.76859219086854 33.79157805686589 18.16780333572317 38-39 17.777776849747156 30.61230114797852 33.97906168030245 17.630860321971873 40-41 17.884591776145193 30.258664677944815 34.26703601663244 17.589707529277554 42-43 17.380823130501597 30.544313417628487 33.560565568157145 18.514297883712775 44-45 18.059250115465147 30.35459701619686 33.151150350237316 18.435002518100678 46-47 18.12191124432371 30.37965589716344 32.71031940949841 18.78811344901444 48-49 17.98255471494755 30.49570343015411 32.64912669136968 18.87261516352866 50-51 17.943682662827165 30.78459374788478 32.09304385539028 19.178679733897784 52-53 17.82022682271838 31.335978957591728 31.78318028218692 19.060613937502975 54-55 17.976678248841957 30.627213445630986 31.787492504319808 19.60861580120725 56-57 18.39461907011777 30.931031039937974 30.96695737757787 19.70739251236639 58-59 18.379499227006203 30.57776325963193 31.182624118659664 19.860113394702203 60-61 18.459409162590543 30.753640111725872 31.36292866116056 19.42402206452303 62-63 18.459743465965563 30.38858441920715 31.712846579017263 19.438825535810032 64-65 18.265064788864134 30.36729557017207 31.64378150502577 19.723858135938027 66-67 18.443888745765626 30.516124749110958 30.92982288392026 20.110163621203156 68-69 18.471479072905975 30.27787265777476 31.167494419246726 20.08315385007253 70-71 18.624579495762386 29.902904026484666 31.44327744426962 20.02923903348333 72-73 18.598691259678223 29.557146169125343 31.524266835985554 20.31989573521088 74-75 18.204811122901717 30.19581220076704 31.20979938336077 20.38957729297048 76-77 18.141852873878285 29.63640496537707 31.619639212907174 20.602102947837466 78-79 18.039385428878692 29.500968048953112 31.57159843808623 20.888048084081966 80-81 18.38734099785908 29.719257079157202 31.05737363810937 20.836028284874345 82-83 18.584597309561683 29.494422042926516 31.188720497994726 20.732260149517074 84-85 18.386190117321508 29.16415319137559 31.103107908716147 21.346548782586755 86-87 18.322819469820576 29.230963307159367 31.1445950721434 21.301622150876657 88-89 18.93620816340682 28.8156238682299 31.006208627080106 21.241959341283174 90-91 18.746049841032864 28.68576723000337 31.287138804565455 21.281044124398313 92-93 19.12784285547182 28.80082048025667 31.075230185652458 20.996106478619055 94-95 18.92562819024892 28.47421188475039 31.127805476386833 21.47235444861386 96-97 19.274734135693873 28.162005660874446 30.702687203127134 21.86057300030455 98-99 19.390839942279364 28.083171558373586 30.40913597958709 22.116852519759956 100-101 19.51461075881629 27.887660873869535 30.89501558570129 21.702712781612888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4635.0 1 3323.0 2 2262.0 3 2573.5 4 2101.0 5 1882.5 6 3176.0 7 5856.0 8 8398.0 9 8795.5 10 6850.5 11 4862.5 12 4187.5 13 3865.0 14 3651.5 15 3510.0 16 3342.0 17 3209.5 18 3252.0 19 3404.5 20 3646.5 21 4076.0 22 4612.0 23 5244.0 24 6067.5 25 7723.0 26 10298.5 27 12686.5 28 14183.0 29 16519.0 30 20482.0 31 24829.0 32 30208.5 33 36038.5 34 42075.0 35 47820.5 36 53273.5 37 56990.0 38 55577.5 39 52252.5 40 49654.0 41 47030.5 42 44254.5 43 43196.0 44 43139.0 45 41635.0 46 39498.5 47 39357.5 48 42740.5 49 40631.0 50 33285.5 51 27389.5 52 22341.0 53 18893.5 54 16184.0 55 13849.0 56 11620.5 57 9377.5 58 7297.5 59 5590.0 60 4221.0 61 3183.0 62 2432.0 63 1764.0 64 1275.0 65 994.0 66 774.0 67 587.5 68 470.5 69 366.0 70 267.5 71 210.0 72 179.0 73 145.0 74 111.5 75 82.0 76 63.0 77 47.5 78 30.5 79 17.0 80 10.0 81 7.0 82 5.0 83 4.5 84 3.0 85 3.5 86 2.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11928508969721925 2 0.11436697628552399 3 0.1240364874000435 4 0.1373737441097256 5 0.13545651345770882 6 0.13537315560327332 7 0.14254193108472743 8 0.14087477399601717 9 0.14887712802182643 10-11 0.1450426667177928 12-13 0.1445841985183975 14-15 0.13170541000811073 16-17 0.13545651345770882 18-19 0.15283662610751328 20-21 0.1853461893373634 22-23 0.20101746597123987 24-25 0.22148181923515833 26-27 0.22194028743455366 28-29 0.22331569203273963 30-31 0.22414927057709477 32-33 0.2254413173208452 34-35 0.19555752650571376 36-37 0.1846793265018793 38-39 0.19743307823051282 40-41 0.18572129968232323 42-43 0.18897225600530823 44-45 0.19305679087264835 46-47 0.2136461809182201 48-49 0.23023439395088724 50-51 0.24707268054686088 52-53 0.26724528132025505 54-55 0.2604516161837607 56-57 0.22952585218818533 58-59 0.22319065525108636 60-61 0.20264294413273237 62-63 0.19180642305611567 64-65 0.1849710789924036 66-67 0.17967785523574853 68-69 0.18572129968232323 70-71 0.18963911884079232 72-73 0.2246077387764901 74-75 0.23648623303355068 76-77 0.2534912353383954 78-79 0.24257135640734315 80-81 0.24715603840129635 82-83 0.24403011885996465 84-85 0.23048446751419374 86-87 0.2213984613807228 88-89 0.22369080237769945 90-91 0.22519124375753868 92-93 0.23231834031177506 94-95 0.23640287517911518 96-97 8.718106242919792 98-99 0.2951701625561519 100-101 0.30667354646825273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1199647.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.424175508615384 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24869546367364 39.96050128889092 2 11.316446801304226 11.41244999285747 3 3.9825337161679313 6.024479372190901 4 1.7686645751100534 3.5673381180487995 5 0.9716386741947084 2.449703951927617 6 0.6053710068261537 1.831520033761751 7 0.3866942733509323 1.3649117935336736 8 0.26419625033910826 1.0657502476653826 9 0.1828798452315757 0.8299408871650853 >10 1.0252763306144836 10.077913287392235 >50 0.141797728338494 4.901554424763222 >100 0.09719916081320157 9.327997202771517 >500 0.0055687008465493855 1.8395616850914613 >1k 0.002362479147020951 1.9457540613805655 >5k 3.3749702100299304E-4 1.198578375327181 >10k+ 3.3749702100299304E-4 2.2020452772321537 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14920 1.2436991881778556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10959 0.9135187267587882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8673 0.7229626715192052 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5413 0.45121606605943254 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2679 0.22331569203273963 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2592 0.21606355869685 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2148 0.17905267132748218 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2090 0.17421791577022241 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1750 0.14587624526214796 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1647 0.1372903862552901 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1606 0.13387271422343408 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1410 0.1175345747540735 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.11169952494358758 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1261 0.10511425444318202 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.335785443551311E-5 0.0 12-13 0.0 0.0 0.0 8.335785443551311E-5 0.0 14-15 0.0 0.0 0.0 1.2503678165326967E-4 0.0 16-17 0.0 0.0 0.0 1.6671570887102623E-4 0.0 18-19 0.0 0.0 0.0 2.917524905242959E-4 0.0 20-21 0.0 0.0 0.0 7.085417627018614E-4 0.0 22-23 0.0 0.0 0.0 0.0028758459780252026 0.0 24-25 0.0 0.0 0.0 0.008669216861293363 0.0 26-27 0.0 0.0 0.0 0.01633813946936057 0.0 28-29 0.0 0.0 0.0 0.034301757100213645 0.0 30-31 0.0 0.0 0.0 0.09361087053108122 0.0 32-33 0.0 0.0 0.0 0.18292881155873353 0.0 34-35 0.0 0.0 0.0 0.3003383495311538 0.0 36-37 0.0 0.0 0.0 0.476973643080006 0.0 38-39 0.0 0.0 0.0 0.7405511788050985 0.0 40-41 0.0 0.0 0.0 1.029094391933627 0.0 42-43 0.0 0.0 0.0 1.297715077852068 0.0 44-45 0.0 0.0 0.0 1.6118491522922993 0.0 46-47 0.0 0.0 0.0 1.9672453646781096 0.0 48-49 0.0 0.0 0.0 2.3525670468062687 0.0 50-51 0.0 0.0 0.0 2.7566859251096365 0.0 52-53 0.0 0.0 0.0 3.120501280793434 0.0 54-55 0.0 0.0 0.0 3.499321050275623 0.0 56-57 0.0 0.0 0.0 3.9318649569415003 0.0 58-59 0.0 0.0 0.0 4.407879984695498 0.0 60-61 0.0 0.0 0.0 4.9229064883253155 0.0 62-63 0.0 0.0 0.0 5.395837275465199 0.0 64-65 0.0 0.0 0.0 5.842510338457897 0.0 66-67 0.0 0.0 0.0 6.352535370821584 0.0 68-69 0.0 0.0 0.0 6.91495081469799 0.0 70-71 0.0 0.0 0.0 7.530090101504859 0.0 72-73 0.0 0.0 0.0 8.111261062629257 0.0 74-75 0.0 0.0 0.0 8.651920106497995 0.0 76-77 0.0 0.0 0.0 9.227297696739125 0.0 78-79 0.0 0.0 0.0 9.8567745345089 0.0 80-81 0.0 0.0 0.0 10.516010126312157 0.0 82-83 0.0 0.0 0.0 11.162325250677908 0.0 84-85 0.0 0.0 0.0 11.783257908368046 0.0 86-87 0.0 8.335785443551311E-5 0.0 12.328668349939608 0.0 88-89 0.0 8.335785443551311E-5 0.0 12.87186980836863 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 395 0.0 35.728603 9 GCACCGT 55 0.0027049948 34.212845 6 GTACATG 9760 0.0 29.111252 1 TACATGG 9965 0.0 28.370756 2 CGGACTC 50 5.246578E-5 28.262169 48-49 CATGGGG 2520 0.0 27.0682 4 GGCATTC 540 0.0 27.005972 7 CGCGGAG 105 7.376145E-5 26.881521 8 GGACCGC 70 0.00879716 26.881521 6 ACATGGG 10115 0.0 26.835012 3 GTATCAA 15455 0.0 26.784739 1 GCATTCC 580 0.0 26.76565 8 CATGGGT 1040 0.0 26.687666 4 ACGCGGA 110 1.0121118E-4 25.659632 7 GCGGAGT 95 0.0012977378 24.759296 9 TAGGCAT 615 0.0 24.477482 5 CCGTATC 130 1.9226718E-9 24.001421 94-95 AGGCTTA 750 0.0 23.83495 6 CATGGGA 6730 0.0 23.62618 4 GAGTACT 9635 0.0 23.508144 12-13 >>END_MODULE