FastQCFastQC Report
Fri 27 May 2016
SRR939850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199647
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152301.2695401230528647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125611.0470580095644804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100320.8362459956970676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65230.543743284482852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32250.2688290805545298No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28940.24123763073637494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.2058105426012819No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.16379818396578325No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.1563793349210226No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.14912720158513296No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17090.14245857323029193No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15490.12912131652060982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.12870452724843226No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13350.11128273567141002No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13180.10986565214600628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG2700.035.1406369
TCGACCG658.398274E-932.84297618-19
TATAACG650.00587328429.2010572
GTACATG103900.027.8168561
GTATCAA150550.026.510721
TACATGG107500.026.4405422
CGTCACT908.994482E-426.3653661
ACATGGG104900.026.0037333
CGCTCCG651.0034721E-525.57438534-35
CGACCGT751.0726981E-625.30125618-19
GGTATCA108850.025.15671
CATGGGT11000.024.5824724
CATGGGG23950.024.5617624
ATGGGTA7200.024.3788155
CATGGGA70200.023.7199274
GTCCTAC5650.022.678881
GACTCCC10250.022.6785669
GAGTACT101250.022.65399412-13
GTGTAGC5050.022.5541341
AGTACTT105900.022.37602212-13
Produced by FastQC (version 0.11.5)