##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1199647 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345434948780767 31.0 31.0 34.0 30.0 34.0 2 31.542927211087928 31.0 31.0 34.0 30.0 34.0 3 31.55910280274114 31.0 31.0 34.0 30.0 34.0 4 35.38617776729321 37.0 35.0 37.0 33.0 37.0 5 35.15906762572657 37.0 35.0 37.0 32.0 37.0 6 35.21489821589184 37.0 35.0 37.0 32.0 37.0 7 35.12058797296205 37.0 35.0 37.0 32.0 37.0 8 35.09612827773503 37.0 35.0 37.0 32.0 37.0 9 36.62091265180507 39.0 35.0 39.0 32.0 39.0 10-11 36.49364313002075 38.0 35.0 39.0 32.0 39.0 12-13 36.41821052359569 38.0 35.0 39.0 32.0 39.0 14-15 37.301184014964406 39.0 36.0 41.0 32.0 41.0 16-17 37.265260947595415 39.0 36.0 40.5 32.0 41.0 18-19 37.39224997019957 39.0 36.0 40.5 32.0 41.0 20-21 37.37687586431675 39.0 36.0 41.0 32.0 41.0 22-23 37.220570301096906 39.0 36.0 40.0 31.0 41.0 24-25 37.06949044177162 39.0 36.0 40.0 31.0 41.0 26-27 36.74292062581743 39.0 35.0 40.0 30.5 41.0 28-29 36.48312045126608 38.0 35.0 40.0 30.0 41.0 30-31 36.12623296686442 38.0 35.0 40.0 30.0 41.0 32-33 35.92341497123738 38.0 34.5 40.0 30.0 41.0 34-35 35.69722885148715 38.0 34.0 40.0 29.5 41.0 36-37 35.5500522236958 38.0 34.0 40.0 28.5 41.0 38-39 35.358529634134044 38.0 34.0 40.0 27.0 41.0 40-41 35.21674125805341 38.0 34.0 40.0 27.0 41.0 42-43 34.89240876691227 38.0 34.0 40.0 25.5 41.0 44-45 34.487060360255974 38.0 33.0 40.0 24.0 41.0 46-47 34.016654482526945 38.0 33.0 40.0 23.0 41.0 48-49 33.600075688931824 38.0 33.0 40.0 20.0 41.0 50-51 33.36081405613484 37.5 33.0 40.0 19.0 41.0 52-53 33.131901717755305 37.0 33.0 40.0 17.0 41.0 54-55 32.89336446471337 37.0 32.0 40.0 14.5 41.0 56-57 32.54098664023667 37.0 31.5 40.0 11.0 41.0 58-59 32.20914610714652 36.5 31.0 40.0 8.5 41.0 60-61 31.924732442126725 36.0 31.0 39.0 7.5 41.0 62-63 31.63801018132834 36.0 31.0 39.0 4.0 40.5 64-65 31.206432808984644 35.5 30.0 39.0 2.0 40.0 66-67 30.331546696653266 35.0 28.5 38.5 2.0 40.0 68-69 28.49882507104173 34.0 24.5 38.0 2.0 40.0 70-71 27.870035518781776 33.0 23.0 37.0 2.0 39.0 72-73 28.357248423911365 33.5 26.0 36.5 2.0 39.0 74-75 28.40344367968244 34.0 26.0 36.0 2.0 39.0 76-77 27.862035248702327 32.5 26.0 35.5 2.0 38.0 78-79 28.32514106232917 34.0 27.0 36.0 2.0 38.0 80-81 28.071439348408326 34.0 26.5 35.5 2.0 37.0 82-83 27.576492084754932 33.0 26.0 35.0 2.0 37.0 84-85 27.16202516240194 33.0 26.0 35.0 2.0 36.5 86-87 26.75937421591518 33.0 25.0 35.0 2.0 36.0 88-89 26.254840382212436 32.5 24.5 35.0 2.0 35.5 90-91 25.73598483553912 32.0 22.5 35.0 2.0 35.0 92-93 25.235013299745674 32.0 20.0 34.5 2.0 35.0 94-95 24.445701110409978 31.0 14.0 34.0 2.0 35.0 96-97 23.912296700612764 31.0 4.5 34.0 2.0 35.0 98-99 23.26917126454699 31.0 2.0 34.0 2.0 35.0 100-101 21.901055893942136 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09470135759496046 1101 2 0.22918440747174884 1101 3 0.2264662723674924 1101 4 0.1367553308803764 1101 5 0.15706017336790978 1101 6 0.04023187451979737 1101 7 0.019667686406947382 1101 8 0.11748977213697032 1101 9 0.16935796471440057 1101 10-11 0.24346800623204246 1101 12-13 0.26739591185882006 1101 14-15 -0.004250318497739158 1101 16-17 0.16610561285614978 1101 18-19 0.03707558399250388 1101 20-21 0.3232446270191929 1101 22-23 0.22153630501826171 1101 24-25 0.0745420132959751 1101 26-27 0.1848612758062913 1101 28-29 0.283922874626505 1101 30-31 -0.013023195458345072 1101 32-33 0.1222057882436971 1101 34-35 0.26731128060757214 1101 36-37 0.4865845040592305 1101 38-39 0.6727985234516396 1101 40-41 0.6630180714892688 1101 42-43 0.40880304888587204 1101 44-45 0.27372529457480965 1101 46-47 0.44387861204796053 1101 48-49 0.49755637968060284 1101 50-51 0.2690198856583095 1101 52-53 0.34667783321452816 1101 54-55 0.3129518094830033 1101 56-57 0.39759925337231294 1101 58-59 0.0660565837519016 1101 60-61 -0.03113686966296214 1101 62-63 0.12639804510184405 1101 64-65 0.10138784232267284 1101 66-67 -0.137835132629462 1101 68-69 -8.620493764844788 1101 70-71 -6.248354088724923 1101 72-73 -1.4722609389066612 1101 74-75 -0.3724742701681727 1101 76-77 -0.2629224115813962 1101 78-79 -0.48974826322505294 1101 80-81 -0.7076187760054502 1101 82-83 -0.47803309055969834 1101 84-85 -0.38729744876056316 1101 86-87 -0.39907788486722495 1101 88-89 -0.3074418727625847 1101 90-91 -0.7441072161667108 1101 92-93 -0.8790572540925226 1101 94-95 -0.7506473338103099 1101 96-97 -1.4348720941188624 1101 98-99 -1.0805629123207794 1101 100-101 -0.7949947767942511 1102 1 0.16340117728142545 1102 2 0.1946280622130523 1102 3 0.22359431674566466 1102 4 0.18858912316063936 1102 5 0.3991592115747835 1102 6 0.3772788685081352 1102 7 0.28632782260353196 1102 8 0.24339288172914308 1102 9 0.38150028542578696 1102 10-11 0.3380506184926375 1102 12-13 0.24960981377842018 1102 14-15 0.5149064526927276 1102 16-17 0.5285401721038383 1102 18-19 0.5153523380586336 1102 20-21 0.3332496699933145 1102 22-23 0.5216901338691571 1102 24-25 0.2837702931338768 1102 26-27 0.45731974383057405 1102 28-29 0.20843136605163437 1102 30-31 0.22440546822606677 1102 32-33 0.23924197067601227 1102 34-35 0.32857470888668416 1102 36-37 0.2076644986897307 1102 38-39 0.5299419507149068 1102 40-41 0.6321888106296498 1102 42-43 0.5964488507006109 1102 44-45 0.2110585408352179 1102 46-47 0.5005725252870405 1102 48-49 0.7222800138146468 1102 50-51 0.5061292819163441 1102 52-53 0.3385912972298257 1102 54-55 0.2417936306134294 1102 56-57 0.08790694735449023 1102 58-59 0.12483698218314032 1102 60-61 0.12919016176673992 1102 62-63 0.21904654980559712 1102 64-65 0.07680642731492782 1102 66-67 0.5810328466616923 1102 68-69 -4.285280381734761 1102 70-71 0.6042256283605987 1102 72-73 0.9807356398526252 1102 74-75 0.7492282959437659 1102 76-77 0.6301487596584145 1102 78-79 0.22400998602848077 1102 80-81 0.40248349841215614 1102 82-83 0.19687865739318156 1102 84-85 0.22520622462885953 1102 86-87 0.24086355858435127 1102 88-89 0.08151909316650219 1102 90-91 0.10987836600655854 1102 92-93 -0.015138101312174967 1102 94-95 0.12722634513001196 1102 96-97 -0.5230885864582611 1102 98-99 -0.10525062886935288 1102 100-101 -0.2742931866247851 1103 1 0.20473216230234925 1103 2 0.24476796532800194 1103 3 0.35078701912453525 1103 4 0.2679751287292973 1103 5 0.26093493400700396 1103 6 0.18278847633309425 1103 7 0.20191079802521017 1103 8 0.285582168068764 1103 9 0.32734490542146233 1103 10-11 0.4027366690362797 1103 12-13 0.4893409251412919 1103 14-15 0.5116994956455585 1103 16-17 0.3609427634871807 1103 18-19 0.40589076764187126 1103 20-21 0.3147563664602018 1103 22-23 0.2626647521825589 1103 24-25 0.21270843707443987 1103 26-27 0.16174480210923292 1103 28-29 0.2734490642248275 1103 30-31 0.21078388297786432 1103 32-33 0.3163177724152675 1103 34-35 0.16502607925057333 1103 36-37 0.2135490125306987 1103 38-39 0.36982446643040134 1103 40-41 0.1991466131036148 1103 42-43 0.020748390994427268 1103 44-45 0.42117825670315057 1103 46-47 0.3389533570153418 1103 48-49 0.44010521560128524 1103 50-51 0.3352720448912905 1103 52-53 0.5275178369365534 1103 54-55 0.2971118372443229 1103 56-57 0.5257879043947895 1103 58-59 0.3268086719724437 1103 60-61 0.4558139820315752 1103 62-63 0.3825944928006564 1103 64-65 0.23736267523354826 1103 66-67 0.4993297708530875 1103 68-69 -3.3632247844085406 1103 70-71 0.6722487544313331 1103 72-73 0.9597185871220226 1103 74-75 0.7037280264371546 1103 76-77 0.5445373229650023 1103 78-79 0.4023677466173403 1103 80-81 0.4851506182619225 1103 82-83 0.5116424700363069 1103 84-85 0.2692937301649039 1103 86-87 0.2717860951737343 1103 88-89 0.37055343551886466 1103 90-91 0.2928486378820807 1103 92-93 0.2976983098896895 1103 94-95 0.13490264880388736 1103 96-97 -0.02243078432761436 1103 98-99 0.11409548285401172 1103 100-101 -0.042680575775168705 1104 1 0.24133244117833996 1104 2 0.2670618384968222 1104 3 0.18810575817291308 1104 4 0.16903627549707068 1104 5 0.22960899009957103 1104 6 0.2645558613473966 1104 7 0.11946681799894776 1104 8 0.24099779795373166 1104 9 0.329008032681422 1104 10-11 0.3216036762512644 1104 12-13 0.3020162777033448 1104 14-15 0.3994627144011531 1104 16-17 0.38007649228872253 1104 18-19 0.26686939952894306 1104 20-21 0.28020241611093155 1104 22-23 0.2321231047884993 1104 24-25 0.3655375428143586 1104 26-27 0.37868466145580015 1104 28-29 0.31137619858835563 1104 30-31 0.23917690229998811 1104 32-33 0.54826730463963 1104 34-35 0.5756885278897883 1104 36-37 0.5977935320773398 1104 38-39 0.6308908801054471 1104 40-41 0.5238112697924109 1104 42-43 0.486250485940559 1104 44-45 0.4529812608660251 1104 46-47 0.4187807645152901 1104 48-49 0.33884250834341856 1104 50-51 0.33514341080967114 1104 52-53 0.35933468361216114 1104 54-55 0.35755601309150364 1104 56-57 0.0978864630853522 1104 58-59 0.20783328801152834 1104 60-61 0.2001900392112823 1104 62-63 0.4853613072333367 1104 64-65 0.4584826357992675 1104 66-67 0.7410430012566422 1104 68-69 -3.6664926302772294 1104 70-71 1.0016607177385417 1104 72-73 1.0147115139356444 1104 74-75 0.8369990121928694 1104 76-77 0.775408489686253 1104 78-79 0.6291030406356874 1104 80-81 0.46676033368011716 1104 82-83 0.18142966672490246 1104 84-85 0.244739813577862 1104 86-87 0.1518042592513993 1104 88-89 0.1956357309450958 1104 90-91 0.35913165311897544 1104 92-93 0.11197466721558413 1104 94-95 0.5066055142040184 1104 96-97 -0.11920007690768486 1104 98-99 0.05539681409858588 1104 100-101 0.14341782760915578 1105 1 0.22903446358095536 1105 2 0.20108941610493147 1105 3 0.16400317000701747 1105 4 0.2640033955340684 1105 5 0.27075352116627727 1105 6 0.24129119272781452 1105 7 0.13254841795604477 1105 8 0.3086205866840501 1105 9 0.27675805111564955 1105 10-11 0.3307249332646549 1105 12-13 0.23476485491595156 1105 14-15 0.3681449686058329 1105 16-17 0.3083207491881481 1105 18-19 0.25015189907647084 1105 20-21 0.1577907495895019 1105 22-23 0.24902461237334705 1105 24-25 0.36376081820834827 1105 26-27 0.16979835708319513 1105 28-29 0.35290114512908843 1105 30-31 0.4398690238452474 1105 32-33 0.3943672919032579 1105 34-35 0.520736423371055 1105 36-37 0.33571743557582323 1105 38-39 0.4727369174163343 1105 40-41 0.5711011028118094 1105 42-43 0.46644810088636035 1105 44-45 0.7808846520940165 1105 46-47 0.826778874168788 1105 48-49 0.6095887259745822 1105 50-51 0.5702240857035861 1105 52-53 0.5232753186881425 1105 54-55 0.5118729745941266 1105 56-57 0.4871533840326734 1105 58-59 0.5184581078588266 1105 60-61 0.7588310007407166 1105 62-63 0.7727901678638496 1105 64-65 0.6551889416466068 1105 66-67 1.2600533847556648 1105 68-69 2.1940564416430846 1105 70-71 2.377840005708027 1105 72-73 1.3978951389767005 1105 74-75 0.8066385056872605 1105 76-77 0.5342284308307832 1105 78-79 0.5265450398446951 1105 80-81 0.5802112885732313 1105 82-83 0.548871800001038 1105 84-85 0.3080419539595489 1105 86-87 0.49232671884691825 1105 88-89 0.7497767293429938 1105 90-91 0.5972596738189395 1105 92-93 0.9033011944077494 1105 94-95 0.9847405063946084 1105 96-97 0.56721188456142 1105 98-99 0.8246867275582588 1105 100-101 0.7175934823594865 1106 1 0.37844348260648175 1106 2 0.28037995501484403 1106 3 0.17706068382685913 1106 4 0.20191845213366832 1106 5 0.28103429036428196 1106 6 0.3182308680928685 1106 7 0.4181016800631312 1106 8 0.49487115807449555 1106 9 0.5612849398272459 1106 10-11 0.4545037192764525 1106 12-13 0.4164577889810488 1106 14-15 0.6914071608712646 1106 16-17 0.6220041662307736 1106 18-19 0.34645212820048243 1106 20-21 0.47199445134782536 1106 22-23 0.530549039492314 1106 24-25 0.6004491953459166 1106 26-27 0.8266737811897684 1106 28-29 0.8249612170746019 1106 30-31 0.7782244414784287 1106 32-33 0.6963311715095131 1106 34-35 0.7315056781643463 1106 36-37 0.658017846409102 1106 38-39 0.592738848077218 1106 40-41 0.6109657010214065 1106 42-43 0.5951344565084185 1106 44-45 0.7434489956267285 1106 46-47 0.4699387981916061 1106 48-49 0.5374813812295329 1106 50-51 0.44377360573561475 1106 52-53 0.348397901458128 1106 54-55 0.3045263840560679 1106 56-57 0.47511301618499147 1106 58-59 0.4725963942837197 1106 60-61 0.5353598428268533 1106 62-63 0.6500555002924209 1106 64-65 0.8302672327845855 1106 66-67 1.3420752409725338 1106 68-69 2.5861807648470716 1106 70-71 3.004215246685739 1106 72-73 1.8921933011578957 1106 74-75 1.5908410149785652 1106 76-77 1.2308172653646245 1106 78-79 1.1452338371849216 1106 80-81 1.2348728408813656 1106 82-83 1.328555001263915 1106 84-85 1.1697235879800623 1106 86-87 1.1454274170079657 1106 88-89 1.3954472223145338 1106 90-91 1.310341462860169 1106 92-93 1.2306206137330982 1106 94-95 1.624012845986499 1106 96-97 1.576967400431169 1106 98-99 1.6173869839386654 1106 100-101 2.05852477070653 1107 1 0.1140443799159847 1107 2 0.04331592883855606 1107 3 0.017522483875438866 1107 4 0.07882273435750875 1107 5 0.0727827579368352 1107 6 0.18653069520361498 1107 7 0.2821392870472863 1107 8 0.17225981596810414 1107 9 0.13469571611427256 1107 10-11 0.12761015332848302 1107 12-13 0.12356267988936054 1107 14-15 0.21307975721057915 1107 16-17 0.10474167462519546 1107 18-19 0.14832033229553332 1107 20-21 0.19101441219769555 1107 22-23 0.054138420377341845 1107 24-25 0.20272676117956934 1107 26-27 0.2337836730268279 1107 28-29 0.18626403045423245 1107 30-31 0.21743315166746413 1107 32-33 0.26574191921979917 1107 34-35 0.2797839298396525 1107 36-37 0.23895502045571249 1107 38-39 0.21841575950362113 1107 40-41 0.14675091827603381 1107 42-43 0.23545265846020413 1107 44-45 0.14320323569988602 1107 46-47 -0.027990817308413796 1107 48-49 0.04821908966109589 1107 50-51 0.04122241765783485 1107 52-53 0.09000634302584842 1107 54-55 -0.08840087386325735 1107 56-57 -0.11367275831598533 1107 58-59 0.03979530265242204 1107 60-61 -0.059530636833653006 1107 62-63 -0.12878199810013413 1107 64-65 -0.27557599524347864 1107 66-67 0.39867718133288577 1107 68-69 1.5700556273215334 1107 70-71 1.6568035845199098 1107 72-73 0.6761560370945716 1107 74-75 0.12847086052131118 1107 76-77 0.06741334749416694 1107 78-79 0.2977364448120561 1107 80-81 0.1989433111115524 1107 82-83 0.23965420540182691 1107 84-85 0.28279601571664514 1107 86-87 0.2331708550750342 1107 88-89 0.18590830794472524 1107 90-91 0.16641330477366623 1107 92-93 0.03954441386741081 1107 94-95 0.40352046883074166 1107 96-97 -0.13372458522330888 1107 98-99 0.15200984246953553 1107 100-101 0.42978729088885004 1108 1 -0.13204841381702437 1108 2 -0.20050791510511345 1108 3 -0.12243602822949029 1108 4 -0.11450956822066871 1108 5 -0.21378521392355054 1108 6 -0.020146021325828656 1108 7 -0.08614437677191944 1108 8 -0.07159237374238359 1108 9 -0.021015311677466286 1108 10-11 -0.01000918879203283 1108 12-13 -0.06805921335018184 1108 14-15 -0.05682283642708086 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0.3804733906129343 2316 74-75 -0.1342429045473743 2316 76-77 -0.5303158366942284 2316 78-79 -0.31894423923616344 2316 80-81 -0.39802893447297194 2316 82-83 -0.305579884870113 2316 84-85 -0.5337444945160463 2316 86-87 -0.5504428258893626 2316 88-89 -0.5659139516571834 2316 90-91 -0.6616354757141387 2316 92-93 -0.7349579690874535 2316 94-95 -0.23809713700048718 2316 96-97 -0.6938731694677749 2316 98-99 -0.9800058569978027 2316 100-101 -1.126296252705206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 35.0 5 92.0 6 274.0 7 573.0 8 1135.0 9 2062.0 10 3534.0 11 5134.0 12 7377.0 13 9062.0 14 10832.0 15 14025.0 16 17256.0 17 19857.0 18 16725.0 19 12304.0 20 11518.0 21 12005.0 22 12804.0 23 14192.0 24 16177.0 25 18365.0 26 22044.0 27 26349.0 28 32044.0 29 37902.0 30 44623.0 31 52167.0 32 61968.0 33 73779.0 34 92600.0 35 117580.0 36 140128.0 37 167710.0 38 115001.0 39 10396.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.987897261793 22.121430917843785 15.479015306011847 20.411656514351368 2 14.487868204030619 23.847039568306336 40.96253946867061 20.702552758992436 3 17.98470716802526 29.833609386761275 31.92364087102289 20.258042574190576 4 10.707233044387223 20.198775139686926 40.207994518387494 28.885997297538356 5 10.267770435803198 41.77203794116103 35.135752433840956 12.824439189194822 6 25.565875266536963 41.02760314292716 19.303361158208336 14.103160432327536 7 24.38559009441944 37.001217858253305 22.288806623948545 16.32438542337871 8 24.828387017180887 36.0451866257324 20.976753995133567 18.149672361953144 9 26.780794683769475 16.993082131660397 22.048652645319834 34.1774705392503 10-11 22.772615611092263 29.381476384303053 28.424945004655537 19.42096299994915 12-13 24.746112814853035 28.159825348623386 28.691148312795345 18.40291352372823 14-15 21.544796094184374 29.043251889930954 28.875660923588352 20.536291092296317 16-17 18.08899894010179 32.162221526645205 29.769885803574148 19.97889372967886 18-19 17.540784914228936 30.175710021364615 34.14070972544423 18.142795338962213 20-21 19.480897297288287 28.83981704618109 33.81715621345279 17.86212944307784 22-23 18.75330826484791 29.550025965971656 33.40174234587341 18.294923423307022 24-25 18.39443402238411 29.71690386868454 34.10574207130356 17.782920037627783 26-27 18.044466599202096 29.857699213598725 34.39163517115939 17.706199016039786 28-29 17.556247796226724 30.055341279559737 34.44546604126047 17.942944882953068 30-31 18.075377424783873 30.12971358979296 34.246980143259286 17.547928842163877 32-33 17.619883596666284 30.42504222163315 34.171097228960456 17.78397695274011 34-35 17.948311829592843 30.377480780011627 33.93767492846559 17.736532461929936 36-37 18.025696635504815 29.975666886688302 33.71446309853325 18.28417337927363 38-39 17.51386666722293 30.893828994111228 33.8866093573668 17.705694981299033 40-41 17.647323891007982 30.518715948339526 34.11220139473598 17.721758765916505 42-43 17.25667907678096 30.696152753305483 33.45623838768373 18.59092978222982 44-45 17.80363557472871 30.616136878512396 33.03458260147609 18.545644945282806 46-47 17.894050460386257 30.61298476559746 32.56504251757086 18.927922256445427 48-49 17.72200746428682 30.6532210491887 32.47907605045277 19.145695436071712 50-51 17.6946987993023 30.894029802801228 31.926560571175163 19.48471082672131 52-53 17.69491745992287 31.347085424746258 31.691777525107383 19.26621959022349 54-55 17.81162068236404 30.663842980118115 31.700955579901834 19.823580757616007 56-57 18.271643748746605 30.946092265665815 30.75684796290328 20.0254160226843 58-59 18.09433057008598 30.776381217254432 31.0274453113374 20.101842901322183 60-61 18.284978293898323 30.951379582717646 31.133781696848185 19.62986042653584 62-63 18.245978624073196 30.68191148784136 31.502295934422598 19.569813953662845 64-65 18.069036001857324 30.545684020283815 31.395233486470648 19.990046491388213 66-67 18.175351978337613 30.654350572887033 30.850412389336938 20.319885059438413 68-69 18.227587041013578 30.265702494730018 31.11007136708158 20.39663909717482 70-71 18.448634334100582 30.01866579523359 31.06664496452411 20.46605490614172 72-73 18.27077884554361 29.67718176625615 31.16839357469963 20.88364581350061 74-75 18.009010988369596 30.257393083626592 30.677550115128362 21.05604581287545 76-77 17.674624934125823 29.80231076600826 31.22490110535199 21.298163194513933 78-79 17.619154402631594 29.683313631960573 31.04862710053035 21.648904864877483 80-81 17.994449100990266 29.843813756876624 30.507145771742934 21.654591370390175 82-83 18.03010303451892 29.72038012864623 30.511821607448525 21.737695229386322 84-85 17.843835337816884 29.23679858357979 30.500506817022384 22.418859261580938 86-87 17.69361482556504 29.52892578173029 30.52531592019376 22.25214347251091 88-89 18.292710882265535 28.945283373532742 30.49911869724739 22.262887046954326 90-91 18.049289179329204 28.722164959410552 30.691139676293073 22.53740618496717 92-93 18.21520789091432 29.037039198237757 30.45671188284501 22.29104102800291 94-95 17.977868336204416 28.471763984533695 30.639757744622457 22.910609934639435 96-97 18.20405570233616 28.277531228974684 30.38204272468896 23.13637034400019 98-99 18.260661497885064 28.231277062006622 30.08546873156699 23.42259270854133 100-101 18.04673090029199 27.89469186825646 30.59375462421662 23.464822607234932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4287.0 1 3064.0 2 2127.5 3 2558.5 4 2154.5 5 1923.0 6 3392.5 7 6407.0 8 8711.0 9 8875.5 10 7054.5 11 4899.0 12 4157.0 13 3870.0 14 3559.0 15 3340.5 16 3172.0 17 3049.5 18 3098.0 19 3293.5 20 3568.5 21 3924.0 22 4454.0 23 5020.0 24 5658.5 25 7174.0 26 9829.5 27 12458.5 28 13861.0 29 15967.5 30 19987.5 31 24390.0 32 30062.5 33 36281.0 34 42322.5 35 47800.5 36 53172.0 37 56714.0 38 54640.0 39 50772.5 40 48102.5 41 45877.5 42 43322.5 43 42691.5 44 42890.0 45 41201.0 46 39285.0 47 39864.0 48 44233.0 49 41538.0 50 33507.0 51 28634.0 52 24550.0 53 21184.5 54 17625.0 55 14348.5 56 11934.0 57 9863.0 58 7869.5 59 6003.5 60 4371.5 61 3128.0 62 2257.5 63 1543.5 64 1073.0 65 870.0 66 712.0 67 567.0 68 414.0 69 304.0 70 205.5 71 163.5 72 151.0 73 120.5 74 95.0 75 85.0 76 63.0 77 34.0 78 22.0 79 9.0 80 7.0 81 5.5 82 4.0 83 2.0 84 2.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020756105754442766 2 0.023173483533072647 3 0.0 4 0.0 5 0.0 6 8.335785443551311E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.917524905242959E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2503678165326967E-4 26-27 4.1678927217756557E-4 28-29 0.0 30-31 3.3343141774205246E-4 32-33 0.002083946360887828 34-35 0.004126213794557899 36-37 0.10669805367745679 38-39 0.09832058930668772 40-41 0.10703148509519883 42-43 0.10807345827564276 44-45 0.10102971957584189 46-47 0.09523634869257373 48-49 0.09502795405648494 50-51 0.09465284371152514 52-53 0.07468863757421976 54-55 0.06180984906393297 56-57 0.03363489426472954 58-59 0.01271207280141575 60-61 0.008294106516333555 62-63 0.0022089831425410977 64-65 0.005835049810485918 66-67 0.15091939545549649 68-69 0.40916202849671607 70-71 0.39015643768541913 72-73 0.17763558780207844 74-75 0.027799844454243623 76-77 0.03259292108428563 78-79 0.005751691956050404 80-81 0.001792193870363532 82-83 4.167892721775656E-5 84-85 2.5007356330653934E-4 86-87 0.0013754045981859665 88-89 2.0839463608878279E-4 90-91 0.001041973180443914 92-93 0.0032926352502027677 94-95 0.07602236324518796 96-97 0.016671570887102624 98-99 0.016879965523191404 100-101 0.009961263605043817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1199647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.05654668191993 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64928465675115 32.88789694252087 2 13.20945740421692 11.639261535434315 3 4.973023360157143 6.572827074511246 4 2.3385092422073113 4.121065663816305 5 1.292439069439647 2.847020109815248 6 0.7844261149543039 2.073546345120083 7 0.5104915884690407 1.5743347548679631 8 0.3386565708893456 1.1936031219620307 9 0.24802304534503106 0.9834334987891775 >10 1.330836478422066 11.266192344995208 >50 0.18260512384011715 5.553709004031981 >100 0.12905885070668693 10.617252794590938 >500 0.009420353286612226 2.7330439287218318 >1k 0.0030145130517159127 2.2558444648871174 >5k 1.8840706573224454E-4 0.5414458345092786 >10k+ 5.652211971967335E-4 3.1395225814264056 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15230 1.2695401230528647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12561 1.0470580095644804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10032 0.8362459956970676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6523 0.543743284482852 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3225 0.2688290805545298 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2894 0.24123763073637494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2469 0.2058105426012819 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.16379818396578325 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1876 0.1563793349210226 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1789 0.14912720158513296 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1709 0.14245857323029193 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1549 0.12912131652060982 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1544 0.12870452724843226 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1335 0.11128273567141002 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1318 0.10986565214600628 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.335785443551311E-5 0.0 5 0.0 0.0 0.0 8.335785443551311E-5 0.0 6 0.0 0.0 0.0 8.335785443551311E-5 0.0 7 0.0 0.0 0.0 8.335785443551311E-5 0.0 8 0.0 0.0 0.0 8.335785443551311E-5 0.0 9 0.0 0.0 0.0 8.335785443551311E-5 0.0 10-11 0.0 0.0 0.0 1.6671570887102623E-4 0.0 12-13 0.0 0.0 0.0 1.6671570887102623E-4 0.0 14-15 0.0 0.0 0.0 2.0839463608878279E-4 0.0 16-17 0.0 0.0 0.0 2.917524905242959E-4 0.0 18-19 0.0 0.0 0.0 4.584681993953221E-4 0.0 20-21 0.0 0.0 0.0 0.0010002942532261574 0.0 22-23 0.0 0.0 0.0 0.0032509563229850113 0.0 24-25 0.0 0.0 0.0 0.008919290424599904 0.0 26-27 0.0 0.0 0.0 0.01721339694093346 0.0 28-29 0.0 0.0 0.0 0.036010593116141666 0.0 30-31 0.0 0.0 0.0 0.09836226823390548 0.0 32-33 0.0 0.0 0.0 0.19193145983776894 0.0 34-35 0.0 0.0 0.0 0.31609298401946573 0.0 36-37 0.0 0.0 0.0 0.5015642101384824 0.0 38-39 0.0 0.0 0.0 0.7765617719212401 0.0 40-41 8.335785443551311E-5 0.0 0.0 1.0766917268163052 0.0 42-43 8.335785443551311E-5 0.0 0.0 1.3568157966468468 0.0 44-45 8.335785443551311E-5 0.0 0.0 1.683495228179623 0.0 46-47 8.335785443551311E-5 0.0 0.0 2.053312349382777 0.0 48-49 8.335785443551311E-5 0.0 0.0 2.4536801242365462 0.0 50-51 8.335785443551311E-5 0.0 0.0 2.8716780853034267 0.0 52-53 8.335785443551311E-5 0.0 0.0 3.2504561758583983 0.0 54-55 8.335785443551311E-5 0.0 0.0 3.6409043660343414 0.0 56-57 8.335785443551311E-5 0.0 0.0 4.083242820596392 0.0 58-59 8.335785443551311E-5 0.0 0.0 4.548004538001596 0.0 60-61 8.335785443551311E-5 0.0 0.0 5.034355939705597 0.0 62-63 8.335785443551311E-5 0.0 0.0 5.470025765912807 0.0 64-65 8.335785443551311E-5 0.0 0.0 5.896026081005496 0.0 66-67 8.335785443551311E-5 0.0 0.0 6.359037283467554 0.0 68-69 8.335785443551311E-5 0.0 0.0 6.875064081350597 0.0 70-71 8.335785443551311E-5 0.0 0.0 7.477991442482663 0.0 72-73 8.335785443551311E-5 0.0 0.0 8.065205848053635 0.0 74-75 8.335785443551311E-5 0.0 0.0 8.622578141736694 0.0 76-77 8.335785443551311E-5 0.0 0.0 9.222254546545775 0.0 78-79 8.335785443551311E-5 0.0 0.0 9.885574673216372 0.0 80-81 8.335785443551311E-5 0.0 0.0 10.581321005262382 0.0 82-83 8.335785443551311E-5 0.0 0.0 11.263438328108185 0.0 84-85 8.335785443551311E-5 0.0 0.0 11.91638040190156 0.0 86-87 8.335785443551311E-5 0.0 0.0 12.573573726271146 0.0 88-89 8.335785443551311E-5 0.0 0.0 13.26010901540203 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 270 0.0 35.140636 9 TCGACCG 65 8.398274E-9 32.842976 18-19 TATAACG 65 0.005873284 29.201057 2 GTACATG 10390 0.0 27.816856 1 GTATCAA 15055 0.0 26.51072 1 TACATGG 10750 0.0 26.440542 2 CGTCACT 90 8.994482E-4 26.365366 1 ACATGGG 10490 0.0 26.003733 3 CGCTCCG 65 1.0034721E-5 25.574385 34-35 CGACCGT 75 1.0726981E-6 25.301256 18-19 GGTATCA 10885 0.0 25.1567 1 CATGGGT 1100 0.0 24.582472 4 CATGGGG 2395 0.0 24.561762 4 ATGGGTA 720 0.0 24.378815 5 CATGGGA 7020 0.0 23.719927 4 GTCCTAC 565 0.0 22.67888 1 GACTCCC 1025 0.0 22.678566 9 GAGTACT 10125 0.0 22.653994 12-13 GTGTAGC 505 0.0 22.554134 1 AGTACTT 10590 0.0 22.376022 12-13 >>END_MODULE