FastQCFastQC Report
Fri 27 May 2016
SRR939849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424209
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104850.43251221326214034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76340.314906841778081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52170.21520421712814364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82200.034.8057751
GTATCAA132800.033.7986151
GTACATG133600.027.8784751
TATCAAC164650.027.7792912
TACATGG135950.027.4663752
TCAACGC166300.027.3901084
ATCAACG166350.027.3248333
CAACGCA169600.026.9690725
AACGCAG173000.026.5487466
ACATGGG137800.025.858633
ACGCAGA192850.023.5208517
CGCAGAG196050.023.1374158
CATGGGA94350.021.9762884
GAGTACT92650.021.8925612-13
CATGGGG45950.021.787724
CGACGTA550.002783184221.71680390-91
ATGGGAT39650.021.539925
GCAGAGT200500.021.3933039
AGTACTT96700.021.27004812-13
GTACTTT99900.020.28147914-15
Produced by FastQC (version 0.11.5)