##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939849_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2424209 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.985874155239916 31.0 30.0 33.0 16.0 34.0 2 29.809837353132508 31.0 30.0 33.0 25.0 34.0 3 30.061354445924422 31.0 30.0 34.0 25.0 34.0 4 33.84558014593626 35.0 35.0 37.0 28.0 37.0 5 33.8190172547004 35.0 35.0 37.0 28.0 37.0 6 33.8594613748237 35.0 35.0 37.0 28.0 37.0 7 33.78180429162667 35.0 35.0 37.0 28.0 37.0 8 33.6529305022793 35.0 33.0 37.0 28.0 37.0 9 34.91742667402027 37.0 34.0 39.0 28.0 39.0 10-11 34.76390236980393 37.0 34.0 39.0 27.0 39.0 12-13 34.65294452747267 37.0 34.0 39.0 27.0 39.0 14-15 35.31928847718988 38.0 34.0 40.0 27.0 41.0 16-17 35.17348194813236 38.0 33.0 40.0 27.0 41.0 18-19 35.06236343483586 38.0 33.5 40.0 26.0 41.0 20-21 35.26780426110125 38.0 34.0 40.0 26.5 41.0 22-23 35.16420345770517 38.0 34.0 40.0 25.5 41.0 24-25 34.96177784176199 38.0 33.5 40.0 25.0 41.0 26-27 34.8608896757664 38.0 33.5 40.0 25.0 41.0 28-29 34.91409486558295 38.0 34.0 40.0 25.0 41.0 30-31 34.68087590632656 38.0 33.5 40.0 24.0 41.0 32-33 34.32831348287215 38.0 33.0 40.0 24.0 41.0 34-35 34.14487158491698 38.0 33.0 40.0 23.5 41.0 36-37 33.941855054576564 38.0 33.0 40.0 22.5 41.0 38-39 33.72247297984621 38.0 33.0 40.0 21.0 41.0 40-41 33.50033866716937 38.0 32.0 40.0 19.5 41.0 42-43 33.0861148935591 37.0 31.0 40.0 16.5 41.0 44-45 32.71939857495785 37.0 31.0 40.0 15.0 41.0 46-47 32.3009957062283 37.0 30.5 40.0 11.0 41.0 48-49 31.924671923914154 37.0 30.0 39.5 9.0 41.0 50-51 31.292948957783757 35.5 29.5 38.5 8.0 40.5 52-53 31.435857634387133 36.0 30.0 39.0 7.5 40.0 54-55 31.54502231449516 36.0 30.0 39.0 6.5 40.0 56-57 31.257413242835085 36.0 29.5 39.0 2.0 40.0 58-59 30.879785323790152 36.0 28.5 39.0 2.0 40.0 60-61 30.488133861395614 35.0 28.0 39.0 2.0 40.0 62-63 30.05906668938198 35.0 28.0 38.5 2.0 40.0 64-65 29.527012316182308 34.5 27.5 38.0 2.0 40.0 66-67 28.946301453381288 34.0 26.0 38.0 2.0 40.0 68-69 28.426801897031154 34.0 26.0 37.0 2.0 39.0 70-71 27.865742598926083 33.0 25.0 36.5 2.0 39.0 72-73 27.2148515247654 32.5 23.5 36.0 2.0 39.0 74-75 26.867143674493413 32.0 23.0 36.0 2.0 38.0 76-77 26.310406198475462 32.0 22.0 35.0 2.0 37.5 78-79 25.649447716760395 31.5 20.0 35.0 2.0 37.0 80-81 24.92442235797326 31.0 17.5 34.5 2.0 36.0 82-83 24.19529298010196 31.0 10.5 34.0 2.0 36.0 84-85 23.4292241304277 30.0 4.5 34.0 2.0 35.0 86-87 22.522805170676293 29.5 2.0 34.0 2.0 35.0 88-89 21.553294291045038 29.0 2.0 34.0 2.0 35.0 90-91 20.15887842178624 27.0 2.0 33.5 2.0 35.0 92-93 18.57797595009341 24.5 2.0 32.0 2.0 34.0 94-95 18.096629044772953 23.5 2.0 32.0 2.0 34.0 96-97 17.79733822455077 24.0 2.0 32.0 2.0 34.0 98-99 16.91500258434813 19.0 2.0 32.0 2.0 34.0 100-101 15.262485412767628 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8296282880536658 1101 2 0.9762133694407602 1101 3 1.129304199486846 1101 4 1.002956203852257 1101 5 1.0229154234872198 1101 6 1.2029511938157356 1101 7 1.0402696881974265 1101 8 1.2014806909458358 1101 9 1.2994148003767307 1101 10-11 1.2541819605882267 1101 12-13 1.3241285810857732 1101 14-15 1.5156771949629118 1101 16-17 1.200342721748278 1101 18-19 1.2006105383203192 1101 20-21 1.3622270768420037 1101 22-23 1.5043923253750293 1101 24-25 1.5121650637788306 1101 26-27 1.4770998428309738 1101 28-29 1.4750663255974032 1101 30-31 1.4682799701573472 1101 32-33 1.612524613993557 1101 34-35 1.4553173876727428 1101 36-37 1.272906639605452 1101 38-39 1.4120980180619043 1101 40-41 1.3070699513689732 1101 42-43 1.4222471248117898 1101 44-45 1.5579483813489432 1101 46-47 1.3691800298978762 1101 48-49 1.1357343008224987 1101 50-51 0.9831035702345758 1101 52-53 1.0183633067149493 1101 54-55 1.0581881125863575 1101 56-57 1.325314900544953 1101 58-59 1.0775385526842491 1101 60-61 1.3479338831632859 1101 62-63 0.8351548012142231 1101 64-65 1.088673342979181 1101 66-67 1.2198718761651968 1101 68-69 1.0443386149031646 1101 70-71 1.057640181319151 1101 72-73 0.814187169894339 1101 74-75 0.7833513945102268 1101 76-77 0.5695966076819445 1101 78-79 0.24790346363915816 1101 80-81 0.23537385723436444 1101 82-83 0.3052902048087809 1101 84-85 0.32953519868618386 1101 86-87 -0.18423654195392203 1101 88-89 -4.110818045296924 1101 90-91 -7.564908178767578 1101 92-93 -10.3992115710265 1101 94-95 -8.336393451625707 1101 96-97 -4.703160505730093 1101 98-99 -3.092755839727513 1101 100-101 -2.2380187132114155 1102 1 1.8572665198571414 1102 2 1.1990750644772596 1102 3 0.9675311256277368 1102 4 1.0973245076777118 1102 5 1.048797814682004 1102 6 1.115004714438733 1102 7 1.022345481983706 1102 8 1.206244221486088 1102 9 1.3699085492590086 1102 10-11 1.2654242178188753 1102 12-13 1.2724125720464414 1102 14-15 1.469383156692082 1102 16-17 1.4673483631991218 1102 18-19 1.4238055179167972 1102 20-21 1.5091324077691866 1102 22-23 1.4727018410556596 1102 24-25 1.6310053960879287 1102 26-27 1.5895386729208738 1102 28-29 1.4283739716329436 1102 30-31 1.5592869772619977 1102 32-33 1.5376615858188174 1102 34-35 1.5732228974355351 1102 36-37 1.690235810946234 1102 38-39 1.6241808026950437 1102 40-41 1.5761240735299609 1102 42-43 1.4786372465825934 1102 44-45 1.6130663315676017 1102 46-47 1.5928708718884792 1102 48-49 1.565216165265106 1102 50-51 1.6421168853591865 1102 52-53 1.7477281151169741 1102 54-55 1.6991471592299376 1102 56-57 1.626181440400913 1102 58-59 1.687002208409428 1102 60-61 1.4646119302783411 1102 62-63 1.4383388880958172 1102 64-65 1.4031273253232293 1102 66-67 1.5483075522451237 1102 68-69 1.4252991247472515 1102 70-71 1.4060566732146498 1102 72-73 1.4827559972204227 1102 74-75 1.1049262859895208 1102 76-77 1.0707427814126191 1102 78-79 1.0514809888793692 1102 80-81 1.0988572499086082 1102 82-83 0.9024467184897027 1102 84-85 1.080409054544674 1102 86-87 0.8483725071283281 1102 88-89 0.521569085041726 1102 90-91 -5.812310997867222 1102 92-93 -10.64361889353215 1102 94-95 -8.284774989286863 1102 96-97 -4.683200012143786 1102 98-99 -3.181698108338608 1102 100-101 -2.2989772721214923 1103 1 2.0994867268213753 1103 2 1.299251527723154 1103 3 1.0746874408290736 1103 4 0.9253529997185268 1103 5 0.8984544419328273 1103 6 0.8990416232380554 1103 7 0.905059241798476 1103 8 0.8691095042502468 1103 9 1.1384252109269681 1103 10-11 1.0543758321055918 1103 12-13 0.9514698217398632 1103 14-15 1.1190296682154397 1103 16-17 1.0469591384010357 1103 18-19 0.9552198800018701 1103 20-21 0.9172600102766992 1103 22-23 1.010264613285564 1103 24-25 0.988150027919815 1103 26-27 0.9492563080649248 1103 28-29 0.817598769717705 1103 30-31 0.879824802688205 1103 32-33 1.0312505216975296 1103 34-35 0.9419683756757777 1103 36-37 0.9085136882051899 1103 38-39 0.9788559547415261 1103 40-41 0.8425998428132857 1103 42-43 0.7871036372259539 1103 44-45 0.5662476687011448 1103 46-47 0.7128466532988469 1103 48-49 0.6879199394962612 1103 50-51 0.6559701079282618 1103 52-53 0.7388318947905113 1103 54-55 0.5368864538620386 1103 56-57 0.5043874900791927 1103 58-59 0.5392205992752288 1103 60-61 0.5435468447412894 1103 62-63 0.6406368737646098 1103 64-65 0.6323763301663554 1103 66-67 0.39688161918757103 1103 68-69 0.43816769068611805 1103 70-71 0.3177519922357064 1103 72-73 0.5243523418342697 1103 74-75 0.34232614176646337 1103 76-77 0.29821768878863963 1103 78-79 0.15504858708030866 1103 80-81 -0.14041342388757627 1103 82-83 -0.49879242854742145 1103 84-85 -0.4184421300898329 1103 86-87 -0.03763537130783945 1103 88-89 -0.8789489466861653 1103 90-91 -6.632497434865467 1103 92-93 -10.055953396385554 1103 94-95 -8.808701346790869 1103 96-97 -5.570111798859294 1103 98-99 -4.139099064422746 1103 100-101 -3.3294504574575914 1104 1 1.9636514600271333 1104 2 1.1668500941749613 1104 3 1.0164302693330107 1104 4 0.757930472504043 1104 5 0.7547681722923798 1104 6 0.8336627733811 1104 7 0.8743139589590925 1104 8 0.8912826375383816 1104 9 1.0562191595698422 1104 10-11 0.9904287613697988 1104 12-13 0.9101611833002252 1104 14-15 0.942624934181886 1104 16-17 0.8363656919072469 1104 18-19 0.9258125821507619 1104 20-21 0.8874699422409051 1104 22-23 0.7902809054265845 1104 24-25 0.9425711695846744 1104 26-27 0.8263077209350129 1104 28-29 0.8503243270834986 1104 30-31 0.8188363360286814 1104 32-33 0.8755852225559906 1104 34-35 0.9692769880800398 1104 36-37 0.890610357578872 1104 38-39 0.8534984271949284 1104 40-41 0.8582436861296898 1104 42-43 0.9437197284939884 1104 44-45 0.6541038152042375 1104 46-47 0.6306238291474884 1104 48-49 0.5288414184528349 1104 50-51 0.6350263119762332 1104 52-53 0.5235967235261008 1104 54-55 0.5138302037514677 1104 56-57 0.5233384944941903 1104 58-59 0.5621823042322625 1104 60-61 0.5876940908487498 1104 62-63 0.5241586632098034 1104 64-65 0.47572602573596967 1104 66-67 0.5244420571561079 1104 68-69 0.6002916838595098 1104 70-71 0.43648727014505084 1104 72-73 0.4300837307918641 1104 74-75 0.1293600541867761 1104 76-77 0.2841322967408466 1104 78-79 -0.08725996023549598 1104 80-81 -0.11172184870356716 1104 82-83 -0.344068575497797 1104 84-85 -0.3487128680226803 1104 86-87 -0.11723412935335986 1104 88-89 -0.4880276846911116 1104 90-91 -3.236053751232742 1104 92-93 -7.896649902937959 1104 94-95 -5.74529943359083 1104 96-97 -3.028010750200833 1104 98-99 -2.168782570974818 1104 100-101 -1.719348579004775 1105 1 1.8613321637237732 1105 2 1.1434727277478096 1105 3 0.8845635687316893 1105 4 0.9030702303408518 1105 5 0.8239337319849227 1105 6 0.7289940781663518 1105 7 0.6104260576159106 1105 8 0.7496555151550055 1105 9 0.8308007964473276 1105 10-11 0.6204828264512372 1105 12-13 0.5918737824710405 1105 14-15 0.8535524467030413 1105 16-17 0.7575111459815034 1105 18-19 0.818398602180622 1105 20-21 0.7487276531269558 1105 22-23 0.7892510706187963 1105 24-25 0.9365356963524007 1105 26-27 0.5963522036851643 1105 28-29 0.7716972120097978 1105 30-31 1.0416366693502752 1105 32-33 1.0692403503325991 1105 34-35 1.141516901214409 1105 36-37 0.9709271725767508 1105 38-39 0.9423461418886561 1105 40-41 0.7403326675783717 1105 42-43 0.6893962164964762 1105 44-45 0.6347009233603202 1105 46-47 0.6345240439857562 1105 48-49 0.5713100183758826 1105 50-51 0.4650862912566005 1105 52-53 0.4927066631680397 1105 54-55 0.44749149824335177 1105 56-57 0.6851911804376059 1105 58-59 0.7122918165899499 1105 60-61 0.5858158343014317 1105 62-63 0.7945345736807887 1105 64-65 0.578702861347157 1105 66-67 0.6544525963915007 1105 68-69 0.6354565803643517 1105 70-71 0.4814816981722423 1105 72-73 0.5523883678560786 1105 74-75 0.45079327723247786 1105 76-77 0.32561957157765775 1105 78-79 0.13601018641670137 1105 80-81 0.07714227955348107 1105 82-83 -0.3080143894743088 1105 84-85 -0.18706734517094858 1105 86-87 -0.09153328016742535 1105 88-89 -0.14576053301583158 1105 90-91 -2.9961839765770115 1105 92-93 -7.789845949329202 1105 94-95 -5.422634528779529 1105 96-97 -2.8173240585439068 1105 98-99 -1.7517199077113617 1105 100-101 -1.3117059388266323 1106 1 1.6549731258557756 1106 2 1.0827584259994047 1106 3 0.7545429962169408 1106 4 0.9107055791497913 1106 5 0.8933273763660239 1106 6 0.9748651568436983 1106 7 0.8321291800311741 1106 8 -0.18339868307811713 1106 9 -0.19607487849657446 1106 10-11 -0.5111842897463461 1106 12-13 0.030030444398349232 1106 14-15 0.7825594852866118 1106 16-17 0.7996237605557681 1106 18-19 0.8907278100749139 1106 20-21 0.7583936614409126 1106 22-23 0.751187256107805 1106 24-25 0.7874957355289283 1106 26-27 0.8375141726098505 1106 28-29 0.5808765182080435 1106 30-31 0.7926275655721753 1106 32-33 0.8070897196021534 1106 34-35 0.9258419538544231 1106 36-37 0.8441625622751587 1106 38-39 0.8082955908995828 1106 40-41 0.6458532986299872 1106 42-43 0.7112631903406808 1106 44-45 0.6519719759982578 1106 46-47 0.7240597240201225 1106 48-49 0.9022617587857624 1106 50-51 0.8109582851121289 1106 52-53 0.8065195337504498 1106 54-55 0.7859331086668533 1106 56-57 0.8263892729990658 1106 58-59 0.6910188451859867 1106 60-61 0.4431019994665739 1106 62-63 0.603279515594604 1106 64-65 0.5849620757236735 1106 66-67 0.6491613124448641 1106 68-69 0.6078542884819136 1106 70-71 0.6267740906931962 1106 72-73 0.7047132139766425 1106 74-75 0.5958332187163329 1106 76-77 0.5717702112872018 1106 78-79 0.4830488597744136 1106 80-81 0.14675055911865442 1106 82-83 -0.0010745070108164612 1106 84-85 0.10830062725352363 1106 86-87 0.28841735892049414 1106 88-89 0.08594194704796365 1106 90-91 -3.273951432219363 1106 92-93 -8.60250888989684 1106 94-95 -5.962894252893022 1106 96-97 -2.999529126835194 1106 98-99 -1.71476760299916 1106 100-101 -1.1910150034765454 1107 1 1.6975891464477897 1107 2 0.9686612180899772 1107 3 0.788529061250081 1107 4 0.7262731168243377 1107 5 0.5908675756478772 1107 6 0.6232101941376627 1107 7 0.60162636233526 1107 8 0.4842125855234727 1107 9 0.44573823897449927 1107 10-11 0.5365018276055693 1107 12-13 0.4960280990033965 1107 14-15 0.6327648991291497 1107 16-17 0.6499867785291116 1107 18-19 0.5753268264683058 1107 20-21 0.6574341940866262 1107 22-23 0.4026260735705094 1107 24-25 0.5172364447696864 1107 26-27 0.5260086278254761 1107 28-29 0.3715451603389184 1107 30-31 0.5062578765714107 1107 32-33 0.6432733095770615 1107 34-35 0.5783227997104277 1107 36-37 0.49880707673936797 1107 38-39 0.2726848991853714 1107 40-41 0.35507521155375343 1107 42-43 0.4123655498339147 1107 44-45 0.4010321899818834 1107 46-47 0.2912572157192628 1107 48-49 0.34681186931322827 1107 50-51 0.26865841039420246 1107 52-53 0.3780876633141723 1107 54-55 0.2769185615242975 1107 56-57 0.32823756287065464 1107 58-59 0.26380740522293067 1107 60-61 0.07288827667289155 1107 62-63 0.17341778615648806 1107 64-65 0.2911666665129431 1107 66-67 0.3274682673301932 1107 68-69 0.5246412678747241 1107 70-71 0.6034526854951103 1107 72-73 0.5969109656501494 1107 74-75 0.3440859778967109 1107 76-77 0.4832459570428469 1107 78-79 0.4638641155133243 1107 80-81 0.4648023436253048 1107 82-83 0.2815181380501919 1107 84-85 0.23712764167553857 1107 86-87 0.41661353063176776 1107 88-89 0.30316740516967045 1107 90-91 -2.9163838853391617 1107 92-93 -4.379572434279918 1107 94-95 -2.9221802217953154 1107 96-97 -1.4928133497326108 1107 98-99 -1.1851193593944878 1107 100-101 -0.678191704045572 1108 1 1.5374091103873795 1108 2 0.8914549562194587 1108 3 -0.48421071925893244 1108 4 -0.5012564157899391 1108 5 0.39818329802452723 1108 6 0.5761633605696872 1108 7 0.4993187241021033 1108 8 0.5354724832953153 1108 9 0.7568123122745334 1108 10-11 0.673868936382739 1108 12-13 0.6410206798305893 1108 14-15 0.6607848634487183 1108 16-17 0.6771739462416875 1108 18-19 0.5976885236507883 1108 20-21 0.6426936149794997 1108 22-23 0.4559930575865181 1108 24-25 0.5028960355150645 1108 26-27 0.5800515861976763 1108 28-29 0.43342780631423494 1108 30-31 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2.0388949133498073 2311 90-91 2.6031296510570066 2311 92-93 3.491619274055161 2311 94-95 3.064834505824141 2311 96-97 2.3146614656371653 2311 98-99 2.190748416547713 2311 100-101 2.18278349072863 2312 1 1.06410088974987 2312 2 0.4067315418714834 2312 3 -0.33474028784238996 2312 4 -0.7932197140797967 2312 5 -0.24844374229071775 2312 6 -0.2643773657630959 2312 7 0.1815948282783495 2312 8 0.08496383891530002 2312 9 0.14965094887096342 2312 10-11 0.03189766827193807 2312 12-13 0.03419070760719478 2312 14-15 0.03106493555505807 2312 16-17 -0.267658734077564 2312 18-19 -0.6688814461734367 2312 20-21 0.08979296988959362 2312 22-23 0.19922329722341914 2312 24-25 0.22028467849283118 2312 26-27 0.10671912744492573 2312 28-29 0.05337621336339282 2312 30-31 0.17530771548938873 2312 32-33 0.3156334792958049 2312 34-35 0.07688307758183299 2312 36-37 0.10752651670667035 2312 38-39 0.16982151272105028 2312 40-41 0.012557229821055671 2312 42-43 0.2554958171077715 2312 44-45 -0.010547378135093766 2312 46-47 0.055517273647133436 2312 48-49 0.08033674187002049 2312 50-51 -0.01359119725649549 2312 52-53 -0.10794651333820582 2312 54-55 -0.12999007372549443 2312 56-57 -0.16144724121185305 2312 58-59 -0.0862164622972088 2312 60-61 -0.10074402383314052 2312 62-63 -0.2077624367794435 2312 64-65 -0.17784858751359778 2312 66-67 -0.011473239200537222 2312 68-69 0.05105911326959145 2312 70-71 0.11266955175911875 2312 72-73 0.16945946011152913 2312 74-75 0.3134201230325644 2312 76-77 0.5900204899970447 2312 78-79 0.6302397255523857 2312 80-81 0.7405623528245648 2312 82-83 0.6702285014925415 2312 84-85 0.6940926107018264 2312 86-87 1.2811833622324222 2312 88-89 1.2962291674737187 2312 90-91 2.147430791658845 2312 92-93 3.1697751064742192 2312 94-95 2.893034544243811 2312 96-97 2.0553374544209078 2312 98-99 1.7706885426468375 2312 100-101 1.6792954573910617 2313 1 -7.251045483218167 2313 2 -2.589687907802112 2313 3 -1.459918538981583 2313 4 -0.6317997998423266 2313 5 -0.47714172123587417 2313 6 -0.0552467415104374 2313 7 -0.17714265879465785 2313 8 -0.08532634182024879 2313 9 -0.044083296354124 2313 10-11 -0.16224912987303952 2313 12-13 -0.31004285541594356 2313 14-15 -0.3174508838548249 2313 16-17 -0.29787179720977974 2313 18-19 -0.5980633542382705 2313 20-21 -0.335605330513566 2313 22-23 -1.1761272717663402 2313 24-25 -0.25076043116443714 2313 26-27 -0.20449675740787399 2313 28-29 -0.4678837065902286 2313 30-31 -6.586657397988915E-4 2313 32-33 -0.15326598908152533 2313 34-35 -0.09805899079793079 2313 36-37 0.010893127793991653 2313 38-39 -0.10204675867716162 2313 40-41 -0.18592049030142022 2313 42-43 -0.0990701893395709 2313 44-45 -0.23906918890408235 2313 46-47 -1.1758296030697295 2313 48-49 -0.7273285746999605 2313 50-51 -0.7152122348296146 2313 52-53 -1.0393493649524643 2313 54-55 -0.8897935707805651 2313 56-57 -0.6412592266568637 2313 58-59 -0.5280491516372869 2313 60-61 -0.39245851448934843 2313 62-63 -0.3598807218116029 2313 64-65 -0.4808907360811254 2313 66-67 -0.2751763586459681 2313 68-69 -0.38026067230387994 2313 70-71 -0.1673826182591398 2313 72-73 -0.39674537367559637 2313 74-75 -0.24174942510837383 2313 76-77 -0.0010555535548064654 2313 78-79 -0.05906399419036745 2313 80-81 0.12134535251351153 2313 82-83 0.2637095467814632 2313 84-85 0.4402694267426064 2313 86-87 1.2004786440813042 2313 88-89 1.586908236144339 2313 90-91 2.2529007409405857 2313 92-93 3.092364247192638 2313 94-95 2.8763551862306898 2313 96-97 1.8980043136459344 2313 98-99 1.4383483829176598 2313 100-101 1.3549662472304416 2314 1 0.5884881604074863 2314 2 0.035674305571358644 2314 3 -1.124098438205884 2314 4 -0.07070415319827106 2314 5 -0.12739624681115913 2314 6 -3.332084381679529 2314 7 -2.7417114714751953 2314 8 -2.495490071900413 2314 9 -3.044842968224561 2314 10-11 -2.531340627227877 2314 12-13 -1.119880390610362 2314 14-15 -1.2414512408701697 2314 16-17 -2.2448740346412137 2314 18-19 -2.929929281788496 2314 20-21 -2.9088909088175825 2314 22-23 -4.002330144725665 2314 24-25 -2.8436738666055206 2314 26-27 -1.7991945723116416 2314 28-29 -1.8907609739419868 2314 30-31 -2.086354077382559 2314 32-33 -2.2119696582256694 2314 34-35 -1.6265987295478865 2314 36-37 -2.048853885752308 2314 38-39 -3.3725475665258173 2314 40-41 -2.553435972799555 2314 42-43 -1.7554533094103277 2314 44-45 -2.4758920134432074 2314 46-47 -2.0352034620405526 2314 48-49 -2.4685593217343005 2314 50-51 -3.287579284645428 2314 52-53 -2.320434296064729 2314 54-55 -2.7187345968142864 2314 56-57 -1.7413821915679364 2314 58-59 -1.1121702599671046 2314 60-61 -1.5258164898268909 2314 62-63 -1.2200936175712336 2314 64-65 -1.5680782335455028 2314 66-67 -1.0611512336905982 2314 68-69 -1.0601918454238692 2314 70-71 -1.0351320751292512 2314 72-73 -2.235134881988376 2314 74-75 -2.3292325759108437 2314 76-77 -1.3239814541057484 2314 78-79 -2.2252343554249947 2314 80-81 -1.306435043996661 2314 82-83 -0.7758516463597864 2314 84-85 -1.6977087659077448 2314 86-87 -0.4756684959417363 2314 88-89 0.7076147335194634 2314 90-91 1.7245412848007362 2314 92-93 2.888788287105328 2314 94-95 2.6575387529763326 2314 96-97 1.7071405596295115 2314 98-99 1.7740728908044545 2314 100-101 1.656176650982351 2315 1 -2.9380481172147235 2315 2 -1.871679430595595 2315 3 -2.809293456216569 2315 4 -3.9360235571256474 2315 5 -4.025221469464704 2315 6 -3.1643643940463555 2315 7 -5.806543171110093 2315 8 -3.2975332502159276 2315 9 -2.788291814529323 2315 10-11 -3.721311278121732 2315 12-13 -3.254685864048337 2315 14-15 -2.1462017942699774 2315 16-17 -2.5943934940580817 2315 18-19 -2.2664040295180214 2315 20-21 -1.627341269772323 2315 22-23 -1.1067802773778013 2315 24-25 -2.461067235896941 2315 26-27 -3.8857883468271908 2315 28-29 -3.136093121731264 2315 30-31 -3.2127198301193935 2315 32-33 -4.1348155984306665 2315 34-35 -3.672974906716952 2315 36-37 -2.9398739745615003 2315 38-39 -1.911092483243472 2315 40-41 -1.6495951844846957 2315 42-43 -2.9981242886906934 2315 44-45 -6.327108790928516 2315 46-47 -3.1974351512224146 2315 48-49 -2.1390331770554525 2315 50-51 -3.2411046227244036 2315 52-53 -3.2014596451947988 2315 54-55 -1.6529755803564754 2315 56-57 -1.1008030414204875 2315 58-59 -2.4377026041798295 2315 60-61 -2.6452504111737802 2315 62-63 -4.653688502136703 2315 64-65 -3.9686269008745434 2315 66-67 -3.428456129221349 2315 68-69 -2.0490299151695375 2315 70-71 -3.357529158888486 2315 72-73 -2.3270811565084806 2315 74-75 -1.2429711143009143 2315 76-77 -2.884912832530837 2315 78-79 -1.5742017151729542 2315 80-81 -1.090111368626328 2315 82-83 -0.5881176719416388 2315 84-85 -2.2469333951305295 2315 86-87 -1.8758061275700193 2315 88-89 -1.3243706436725873 2315 90-91 -0.1274206614711879 2315 92-93 0.41555826286833053 2315 94-95 0.30884273009614915 2315 96-97 -0.059695522479337626 2315 98-99 -0.12312274450735572 2315 100-101 -0.4453900561529913 2316 1 -7.190966342152617 2316 2 -6.106593175860066 2316 3 -3.421862682369568 2316 4 -3.9905907531409994 2316 5 -1.9945505656408429 2316 6 -2.7592840359545185 2316 7 -3.216168488759781 2316 8 -2.3143559202778228 2316 9 -6.834054652982438 2316 10-11 -6.629902593969 2316 12-13 -5.854517177733328 2316 14-15 -4.693257624622127 2316 16-17 -4.643549775224052 2316 18-19 -3.7520259334862907 2316 20-21 -2.6608800629241713 2316 22-23 -3.0747467213491078 2316 24-25 -3.0238156333181934 2316 26-27 -3.935205772532221 2316 28-29 -3.953815082185102 2316 30-31 -6.516635405223649 2316 32-33 -4.746802080544747 2316 34-35 -5.368403793533513 2316 36-37 -4.234009164388773 2316 38-39 -6.430878229817846 2316 40-41 -3.649039730050113 2316 42-43 -4.323864888074866 2316 44-45 -2.9267756488400316 2316 46-47 -4.106984541097372 2316 48-49 -4.983275932318364 2316 50-51 -4.625775229365779 2316 52-53 -6.312448685741931 2316 54-55 -5.200498075999931 2316 56-57 -4.436382914175198 2316 58-59 -3.14139508350436 2316 60-61 -3.2480211677904656 2316 62-63 -4.017758591241829 2316 64-65 -2.9249143734837446 2316 66-67 -3.989707776716962 2316 68-69 -3.090650413657901 2316 70-71 -2.6180528268097802 2316 72-73 -2.1557601542461846 2316 74-75 -2.239875115218055 2316 76-77 -3.1070891876163813 2316 78-79 -5.0335701710540235 2316 80-81 -6.094424888836432 2316 82-83 -3.7748919675891166 2316 84-85 -2.439421663864973 2316 86-87 -2.403429082906211 2316 88-89 -1.4513212301640515 2316 90-91 -1.1288427893447839 2316 92-93 -1.0615221908024068 2316 94-95 -1.6393238002095458 2316 96-97 -3.4907719054290034 2316 98-99 -4.800140027314097 2316 100-101 -4.070619047270682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18371.0 3 5939.0 4 5578.0 5 6342.0 6 7104.0 7 8492.0 8 9949.0 9 12503.0 10 14716.0 11 17587.0 12 21177.0 13 24004.0 14 26857.0 15 30154.0 16 34486.0 17 37444.0 18 33761.0 19 31424.0 20 34028.0 21 36704.0 22 40829.0 23 46203.0 24 51774.0 25 58920.0 26 66802.0 27 75519.0 28 86636.0 29 99234.0 30 113082.0 31 128820.0 32 145080.0 33 163773.0 34 187968.0 35 220889.0 36 245902.0 37 208204.0 38 66710.0 39 1244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5298478394038 19.71474586821836 13.614401744123846 22.141004548254 2 15.469765686080555 20.66888068464121 40.638326080159786 23.22302754911845 3 19.27256055627722 25.760079633389026 29.825512836134067 25.141846974199687 4 12.415929123118346 16.84945189216787 37.69853367007122 33.03608531464256 5 13.260307670957449 38.12588852111262 34.003315768735455 14.610488039194468 6 30.88728509589229 36.6404506960334 18.06506060627341 14.4072036018009 7 27.99435219680867 33.606040521376364 20.933110638762077 17.466496643052885 8 26.396468010140506 34.816756955071746 20.069269771177563 18.717505263610192 9 27.326965374274266 16.540551216728637 20.273232465765368 35.85925094323172 10-11 24.859443118956932 27.12587182451973 27.294116577665385 20.720568478857952 12-13 26.13973795338075 24.56390115609289 27.830095431835307 21.466265458691066 14-15 22.93130353193541 25.577290705135226 26.74699357668419 24.74441218624517 16-17 21.874564274236576 29.13733167171411 27.09267658474041 21.8954274693089 18-19 21.68716068553144 27.38543313870248 29.895208411755203 21.032197764010878 20-21 22.944023556849576 26.38102278578093 29.71291949068147 20.962034166688014 22-23 22.320679544289156 26.66139497220063 29.578422207750094 21.439503275760117 24-25 21.970675473754454 26.608950087197115 29.99318216961637 21.42719226943206 26-27 21.797485334148206 26.906043856758245 29.89034363185497 21.40612717723858 28-29 21.503052625986076 27.189944641112028 29.923652822430842 21.383349910471054 30-31 21.679246152818894 26.919011620563477 29.839253661026344 21.562488565591284 32-33 21.577559833903642 27.025325208638552 29.680121667834985 21.716993289622824 34-35 22.02149282287552 26.962270668016313 29.554378115180846 21.461858393927326 36-37 21.992169758996518 26.730542591173478 29.61337280633663 21.663914843493373 38-39 21.66547161188534 27.027405785828957 29.72687227947622 21.58025032280948 40-41 21.85193545306506 26.841432706145092 29.73879352149394 21.56783831929591 42-43 21.62661237032984 26.83309560428272 29.58409025284712 21.95620177254032 44-45 21.724619059320865 27.059948335046336 29.29318962162314 21.922242984009657 46-47 21.779895211032134 27.20924699491889 28.917095914751627 22.093761879297347 48-49 21.821727539041703 27.2255418526743 28.835182950041638 22.117547658242362 50-51 21.718390906801247 27.475813518953608 28.665255297493818 22.140540276751324 52-53 21.88700212847309 27.542292882280943 28.511510153000675 22.059194836245293 54-55 21.42771327619624 27.348633811079516 28.71934095551855 22.5043119572057 56-57 21.613403656053755 27.71243597440465 28.47067516096328 22.203485208578314 58-59 21.776361951645896 27.208415183915207 28.50101693878066 22.51420592565824 60-61 21.647325706075268 27.282493764513987 28.817617924469808 22.252562604940934 62-63 21.607526141736383 27.188067158605396 28.77227766969344 22.43212902996478 64-65 21.377522192733405 27.177289322481485 28.719300413784893 22.725888071000213 66-67 21.439284914870964 27.438981919703803 28.334181761137707 22.787551404287527 68-69 21.477900666675485 27.209685313297793 28.536277761610602 22.776136258416116 70-71 21.433910021442365 27.24093784194824 28.42772947814487 22.897422658464524 72-73 21.540567575133675 27.111349476476054 28.402119981491307 22.945962966898964 74-75 21.40125544813213 27.310049374343535 28.257776252346726 23.030918925177605 76-77 21.572080015749048 27.13437229623429 28.230788064471835 23.062759623544828 78-79 21.602357172707897 27.25583301609214 28.174719285486677 22.967090525713285 80-81 21.506078614989065 27.40234756309417 28.138060170677715 22.953513651239053 82-83 21.653147844099205 27.274056496759187 28.00049717114993 23.07229848799168 84-85 21.639965694398956 27.088378274663143 27.96104974477559 23.310606286162304 86-87 21.5466116501522 26.875468068380265 28.336027504948962 23.24189277651857 88-89 21.595947273197208 26.878966141121737 28.091434479193587 23.433652106487465 90-91 21.439073284727897 26.94794932098354 28.383596556385438 23.229380837903125 92-93 21.862105803587635 26.591809323286437 28.310928335468212 23.235156537657716 94-95 21.571605865956645 26.448251948975955 28.4343645575792 23.545777627488203 96-97 21.987858975806894 26.340495577842503 27.96609713729476 23.705548309055835 98-99 21.947712642791064 26.334966748554685 27.698399117901985 24.01892149075227 100-101 22.05741596098236 26.380241515490976 27.911945002463746 23.650397521062917 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3543.0 1 2117.0 2 716.5 3 752.5 4 693.5 5 687.0 6 985.0 7 1764.5 8 2744.5 9 3205.0 10 2974.5 11 2711.5 12 2767.5 13 2971.0 14 3140.0 15 3259.5 16 3511.0 17 3853.0 18 4139.0 19 4507.0 20 5131.5 21 5915.0 22 6997.5 23 8189.0 24 9462.5 25 11519.0 26 14372.0 27 17748.5 28 21327.0 29 25324.5 30 29298.5 31 33323.5 32 38070.5 33 42919.0 34 48100.0 35 53134.0 36 58015.5 37 63058.0 38 67202.5 39 69954.5 40 72788.0 41 77062.0 42 81335.5 43 84944.0 44 88055.0 45 90706.0 46 92970.0 47 96048.5 48 101423.0 49 101846.5 50 96086.0 51 90785.0 52 85744.0 53 81509.5 54 76992.0 55 69914.5 56 61976.5 57 54362.0 58 47327.5 59 40986.5 60 34705.5 61 28378.5 62 22611.5 63 17381.5 64 12928.0 65 9580.0 66 7144.5 67 5458.5 68 3947.5 69 2696.0 70 1980.0 71 1523.0 72 1209.5 73 948.0 74 738.0 75 572.0 76 389.0 77 256.0 78 170.5 79 112.5 80 76.5 81 55.0 82 36.5 83 27.0 84 17.0 85 15.5 86 12.0 87 8.0 88 8.5 89 4.0 90 2.0 91 5.5 92 5.0 93 1.5 94 2.0 95 2.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13530186547447023 2 0.1225141891643831 3 0.1284542710632623 4 0.13757064675529215 5 0.12585548523250265 6 0.14107694509838056 7 0.17131361198642528 8 0.1574534208890405 9 0.16797231591830572 10-11 0.161929107597571 12-13 0.15202897109943903 14-15 0.15708216577036055 16-17 0.1517402171182435 18-19 0.16797231591830572 20-21 0.1728811335986295 22-23 0.20623221842671155 24-25 0.22898190708804397 26-27 0.24073831917957567 28-29 0.23585012678362305 30-31 0.2278062658788908 32-33 0.22656874881662434 34-35 0.21897864416805646 36-37 0.22727000848524198 38-39 0.2150185895688037 40-41 0.21937052457110753 42-43 0.21196604748187964 44-45 0.20491220022696063 46-47 0.22058741634900292 48-49 0.25515539295498035 50-51 0.2568260409890401 52-53 0.2589916958480065 54-55 0.2567229145671846 56-57 0.23285946054981232 58-59 0.23413822818082103 60-61 0.20588158859240271 62-63 0.19808523110012377 64-65 0.19948775043735914 66-67 0.20742848492023583 68-69 0.20794411702951357 70-71 0.21310043812229063 72-73 0.24280084761668652 74-75 0.24539963344744614 76-77 0.2592598245448309 78-79 0.25688791684215345 80-81 0.270314976967745 82-83 0.2693868391710451 84-85 0.2452346311724773 86-87 0.24857592724059682 88-89 0.2509684602276454 90-91 0.4269433864819411 92-93 0.5711760000891013 94-95 0.33547025029607597 96-97 0.2866914527584049 98-99 0.31752625289321174 100-101 0.33716152361450685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2424209.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.35454579069032 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61360335577312 42.61170272712011 2 12.89208772219051 14.53055495755563 3 4.905018530202705 8.292602740934784 4 2.341264526947057 5.277635959678274 5 1.2680842111232289 3.5731154871097703 6 0.7959582311345534 2.6913518750369434 7 0.4783774816701381 1.8871121987210442 8 0.34286204139512766 1.5457467689353024 9 0.23257767743328778 1.1796128435525954 >10 1.0271684942568824 10.040063644386322 >50 0.06376326624491381 2.4986678135474363 >100 0.03624378109510038 3.7312262180253417 >500 0.0024673114400921505 0.9013014589132109 >1k 2.9906805334410413E-4 0.25605202962973617 >5k 1.4953402667205207E-4 0.5414718829639197 >10k+ 7.476701333602603E-5 0.4417813938897126 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10485 0.43251221326214034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7634 0.314906841778081 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5217 0.21520421712814364 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.125056874221653E-5 0.0 10-11 0.0 0.0 0.0 6.18758531133248E-5 0.0 12-13 0.0 0.0 0.0 8.250113748443306E-5 0.0 14-15 0.0 0.0 0.0 8.250113748443306E-5 0.0 16-17 0.0 0.0 0.0 1.6500227496886612E-4 0.0 18-19 2.0625284371108265E-5 0.0 0.0 2.681286968244075E-4 0.0 20-21 4.125056874221653E-5 0.0 0.0 3.0937926556662396E-4 0.0 22-23 4.125056874221653E-5 0.0 0.0 9.28137796699872E-4 0.0 24-25 4.125056874221653E-5 0.0 4.125056874221653E-5 0.001629397465317553 0.0 26-27 4.125056874221653E-5 0.0 4.125056874221653E-5 0.003238169646263998 0.0 28-29 4.125056874221653E-5 0.0 4.125056874221653E-5 0.010023888204358618 0.0 30-31 4.125056874221653E-5 0.0 4.125056874221653E-5 0.033742965231133126 2.0625284371108265E-5 32-33 4.125056874221653E-5 0.0 4.125056874221653E-5 0.08726557817415909 4.125056874221653E-5 34-35 4.125056874221653E-5 0.0 4.125056874221653E-5 0.16875607672440784 4.125056874221653E-5 36-37 4.125056874221653E-5 0.0 4.125056874221653E-5 0.30123227824003623 4.125056874221653E-5 38-39 4.125056874221653E-5 0.0 4.125056874221653E-5 0.5022050491521152 4.125056874221653E-5 40-41 4.125056874221653E-5 0.0 4.125056874221653E-5 0.7197811739829363 4.125056874221653E-5 42-43 4.125056874221653E-5 0.0 4.125056874221653E-5 0.9392341996915283 4.125056874221653E-5 44-45 4.125056874221653E-5 0.0 4.125056874221653E-5 1.1953589810119507 4.125056874221653E-5 46-47 4.125056874221653E-5 0.0 4.125056874221653E-5 1.495621870886545 4.125056874221653E-5 48-49 4.125056874221653E-5 0.0 4.125056874221653E-5 1.815870661316743 4.125056874221653E-5 50-51 4.125056874221653E-5 0.0 4.125056874221653E-5 2.1562909798618852 4.125056874221653E-5 52-53 4.125056874221653E-5 0.0 4.125056874221653E-5 2.480932955863129 4.125056874221653E-5 54-55 4.125056874221653E-5 0.0 4.125056874221653E-5 2.8244676923483083 4.125056874221653E-5 56-57 4.125056874221653E-5 0.0 4.125056874221653E-5 3.214223691109141 4.125056874221653E-5 58-59 4.125056874221653E-5 0.0 4.125056874221653E-5 3.6378670320917053 4.125056874221653E-5 60-61 4.125056874221653E-5 0.0 4.125056874221653E-5 4.090076391928253 4.125056874221653E-5 62-63 4.125056874221653E-5 0.0 4.125056874221653E-5 4.526259905808452 4.125056874221653E-5 64-65 4.125056874221653E-5 0.0 4.125056874221653E-5 4.954770813902597 4.125056874221653E-5 66-67 4.125056874221653E-5 0.0 4.125056874221653E-5 5.439320619633043 4.125056874221653E-5 68-69 4.125056874221653E-5 0.0 4.125056874221653E-5 5.968194161477001 4.125056874221653E-5 70-71 4.125056874221653E-5 0.0 4.125056874221653E-5 6.533718833648419 4.125056874221653E-5 72-73 4.125056874221653E-5 0.0 4.125056874221653E-5 7.0955103293486665 4.125056874221653E-5 74-75 4.125056874221653E-5 0.0 4.125056874221653E-5 7.632902113637892 4.125056874221653E-5 76-77 8.250113748443306E-5 0.0 4.125056874221653E-5 8.204263741286333 4.125056874221653E-5 78-79 8.250113748443306E-5 0.0 4.125056874221653E-5 8.821929132347911 4.125056874221653E-5 80-81 8.250113748443306E-5 0.0 4.125056874221653E-5 9.454692231569142 4.125056874221653E-5 82-83 8.250113748443306E-5 0.0 4.125056874221653E-5 10.049360430556938 4.125056874221653E-5 84-85 8.250113748443306E-5 0.0 4.125056874221653E-5 10.610017535616773 4.125056874221653E-5 86-87 8.250113748443306E-5 0.0 4.125056874221653E-5 11.175129702100769 4.125056874221653E-5 88-89 8.250113748443306E-5 0.0 4.125056874221653E-5 11.780131168558487 4.125056874221653E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8220 0.0 34.805775 1 GTATCAA 13280 0.0 33.798615 1 GTACATG 13360 0.0 27.878475 1 TATCAAC 16465 0.0 27.779291 2 TACATGG 13595 0.0 27.466375 2 TCAACGC 16630 0.0 27.390108 4 ATCAACG 16635 0.0 27.324833 3 CAACGCA 16960 0.0 26.969072 5 AACGCAG 17300 0.0 26.548746 6 ACATGGG 13780 0.0 25.85863 3 ACGCAGA 19285 0.0 23.520851 7 CGCAGAG 19605 0.0 23.137415 8 CATGGGA 9435 0.0 21.976288 4 GAGTACT 9265 0.0 21.89256 12-13 CATGGGG 4595 0.0 21.78772 4 CGACGTA 55 0.0027831842 21.716803 90-91 ATGGGAT 3965 0.0 21.53992 5 GCAGAGT 20050 0.0 21.393303 9 AGTACTT 9670 0.0 21.270048 12-13 GTACTTT 9990 0.0 20.281479 14-15 >>END_MODULE