FastQCFastQC Report
Fri 27 May 2016
SRR939849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424209
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115290.4755778070290144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93690.38647657854582673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65960.27208875142366024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84300.037.6671331
GTATCAA133650.032.6548651
GTACATG132450.027.179891
ATCAACG161750.026.7991373
TATCAAC162950.026.6312772
TCAACGC163050.026.5248744
TACATGG133850.026.2188172
CAACGCA166300.026.0064985
AACGCAG169850.025.474416
ACATGGG134250.024.9455833
ACGCAGA192500.022.4770327
CGCAGAG194000.022.2308148
CATGGGG45450.021.5841144
GAGTACT99750.021.54727612-13
CATGGGA90250.021.1647224
GCAGAGT202800.020.847819
AGTACTT103050.020.26264812-13
GTACTTT106700.020.1879514-15
AGAGTAC178500.018.90648510-11
ATGGGAG35500.018.7324925
Produced by FastQC (version 0.11.5)