##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939849_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2424209 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.458596597900595 31.0 31.0 34.0 30.0 34.0 2 31.664266983581037 31.0 31.0 34.0 30.0 34.0 3 31.72079346293987 31.0 31.0 34.0 30.0 34.0 4 35.52407940074474 37.0 35.0 37.0 33.0 37.0 5 35.26247571888398 37.0 35.0 37.0 33.0 37.0 6 35.29459712425785 37.0 35.0 37.0 32.0 37.0 7 35.21565426083312 37.0 35.0 37.0 32.0 37.0 8 35.160492762793965 37.0 35.0 37.0 32.0 37.0 9 36.76430786289466 39.0 35.0 39.0 32.0 39.0 10-11 36.61915185530621 39.0 35.0 39.0 32.0 39.0 12-13 36.56125276327248 38.5 35.0 39.0 32.0 39.0 14-15 37.59068793160986 39.0 36.0 41.0 32.0 41.0 16-17 37.531216574148516 39.0 36.0 41.0 32.0 41.0 18-19 37.533499380622715 39.0 36.0 41.0 32.0 41.0 20-21 37.477561546879826 39.0 36.0 41.0 32.0 41.0 22-23 37.33283103890795 39.0 36.0 41.0 31.5 41.0 24-25 37.17335015256523 39.0 36.0 40.5 31.0 41.0 26-27 36.89695814181038 39.0 36.0 40.0 31.0 41.0 28-29 36.66625835478707 38.5 35.0 40.0 30.0 41.0 30-31 36.405282094076874 38.0 35.0 40.0 30.0 41.0 32-33 36.3446555144379 38.0 35.0 40.0 30.0 41.0 34-35 36.40258966945507 38.0 35.0 40.0 30.0 41.0 36-37 36.35555618348088 38.0 35.0 40.0 30.0 41.0 38-39 36.225763331461934 38.0 35.0 40.0 30.0 41.0 40-41 36.045804631531354 38.0 35.0 40.0 29.5 41.0 42-43 35.821788467908505 38.0 35.0 40.0 29.0 41.0 44-45 35.54676948233424 38.0 34.5 40.0 27.5 41.0 46-47 35.201384245335284 38.0 34.0 40.0 26.5 41.0 48-49 34.875179285284396 38.0 34.0 40.0 26.0 41.0 50-51 34.60639965448524 38.0 33.0 40.0 24.5 41.0 52-53 34.32441241658619 38.0 33.0 40.0 24.0 41.0 54-55 34.02997245699525 38.0 33.0 40.0 23.5 41.0 56-57 32.695745911346755 36.5 31.5 40.0 15.5 41.0 58-59 29.33657576553837 34.5 23.5 39.0 7.0 40.0 60-61 29.31315348635369 34.0 24.0 38.0 7.5 40.0 62-63 31.269195642784926 35.0 29.5 39.0 8.0 40.0 64-65 30.885373332084818 35.0 29.5 39.0 2.0 40.0 66-67 25.44560803132073 28.5 18.0 36.0 2.0 39.0 68-69 17.771349128726115 13.5 7.0 29.5 2.0 35.5 70-71 16.621110019804398 15.0 7.5 26.5 2.0 33.5 72-73 18.80650410092529 19.5 7.5 30.0 2.0 35.0 74-75 19.505528813728517 20.0 7.5 31.5 2.0 35.5 76-77 20.408968038646833 23.5 9.0 31.5 2.0 34.5 78-79 23.46748712672876 29.0 12.0 34.0 2.0 36.0 80-81 26.11918753704817 31.5 22.0 35.0 2.0 36.0 82-83 27.121161583015326 33.0 26.0 35.0 2.0 36.0 84-85 27.109630811534814 33.0 26.0 35.0 2.0 36.0 86-87 26.819089236942855 33.0 25.5 35.0 2.0 36.0 88-89 26.384443338012524 33.0 25.0 35.0 2.0 35.0 90-91 24.748534057913325 32.0 12.5 34.5 2.0 35.0 92-93 22.62982791500238 31.0 2.0 34.0 2.0 35.0 94-95 18.257228852792807 24.0 2.0 33.0 2.0 35.0 96-97 16.959126255203245 15.0 2.0 32.5 2.0 35.0 98-99 15.889757648783583 4.5 2.0 32.0 2.0 34.0 100-101 15.266335122095496 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3942851415176847 1101 2 0.5260697211893763 1101 3 0.5531454679354404 1101 4 0.3542416388706684 1101 5 0.475738707524215 1101 6 0.58642104227701 1101 7 0.5724376221270262 1101 8 0.549205330860751 1101 9 0.6942232351390913 1101 10-11 0.6784536553309692 1101 12-13 0.6904937024505458 1101 14-15 0.8217675833191649 1101 16-17 0.6901387194929143 1101 18-19 0.7505567023037614 1101 20-21 0.8339764592431109 1101 22-23 0.7911640657747725 1101 24-25 0.744925432724699 1101 26-27 0.6056661703737518 1101 28-29 0.7556589200738202 1101 30-31 0.7830408346591966 1101 32-33 0.7155433116603405 1101 34-35 1.1071677556855022 1101 36-37 0.9275622587660592 1101 38-39 0.8237552902787186 1101 40-41 0.8768049017261248 1101 42-43 0.9828264819068124 1101 44-45 0.9595924356329704 1101 46-47 0.925792197349466 1101 48-49 0.9240550525907452 1101 50-51 0.6758598266659064 1101 52-53 0.7125753815177944 1101 54-55 0.6743754266404736 1101 56-57 1.1275366501607422 1101 58-59 -8.32403601973624 1101 60-61 -9.790625288086755 1101 62-63 -3.1031792000834457 1101 64-65 -0.47058108201093063 1101 66-67 -1.141182635216456 1101 68-69 -3.5389496085388004 1101 70-71 -4.5799319096115525 1101 72-73 -6.904118403034712 1101 74-75 -7.6906708546635905 1101 76-77 -8.722077512782604 1101 78-79 -11.934175519384363 1101 80-81 -7.588898213399272 1101 82-83 -3.8523545655896214 1101 84-85 -2.9450824611420714 1101 86-87 -2.6965600133845022 1101 88-89 -2.845734399478726 1101 90-91 -8.69205679142186 1101 92-93 -14.457855523284998 1101 94-95 -12.66897539239824 1101 96-97 -12.876532827231983 1101 98-99 -12.398941175777829 1101 100-101 -12.078411419975318 1102 1 0.5448484737129746 1102 2 0.5309633139969385 1102 3 0.4083942933105824 1102 4 0.43991202856973644 1102 5 0.5458097540377338 1102 6 0.5275353507521885 1102 7 0.5271353086444464 1102 8 0.6190568972981794 1102 9 0.7063840571326168 1102 10-11 0.7717530098969689 1102 12-13 0.7848782640594152 1102 14-15 0.8967606737612144 1102 16-17 0.9866034644895834 1102 18-19 0.8088855647466602 1102 20-21 0.8250509361489335 1102 22-23 0.8368850171802507 1102 24-25 0.871577629964122 1102 26-27 0.964585190354569 1102 28-29 0.8804577300040748 1102 30-31 0.9369573346835836 1102 32-33 0.8078143614254216 1102 34-35 0.8449703536792939 1102 36-37 0.9252983624256856 1102 38-39 0.8263606000707782 1102 40-41 0.8009664065465572 1102 42-43 0.8918601192606914 1102 44-45 0.8224377103082219 1102 46-47 0.7835772369371696 1102 48-49 0.7217513246810086 1102 50-51 0.7803038917620668 1102 52-53 0.7702498681782828 1102 54-55 0.7800550832760891 1102 56-57 1.3944772604779345 1102 58-59 -8.94342505223555 1102 60-61 -9.972675882308739 1102 62-63 -2.8632143168956183 1102 64-65 -0.02072821495082522 1102 66-67 -1.1399185925560325 1102 68-69 -3.8514323346439223 1102 70-71 -4.346673362081923 1102 72-73 -6.818529302252712 1102 74-75 -7.668048149043276 1102 76-77 -8.599871819306854 1102 78-79 -11.634146255374217 1102 80-81 -7.5210057098632035 1102 82-83 -3.2870836296290307 1102 84-85 -2.278509276730034 1102 86-87 -2.038900158729085 1102 88-89 -2.187517715741386 1102 90-91 -7.971211061528674 1102 92-93 -14.17522208752763 1102 94-95 -12.482084043164795 1102 96-97 -12.47705876652542 1102 98-99 -11.998995639352266 1102 100-101 -11.707697378127783 1103 1 0.21739231888436805 1103 2 0.28692168452479194 1103 3 0.24986428006549133 1103 4 0.20766261613970016 1103 5 0.16909000642217364 1103 6 0.18539955982949152 1103 7 0.22075654386350863 1103 8 0.30197794079166584 1103 9 0.2729286843503118 1103 10-11 0.36250025454435786 1103 12-13 0.39654554299379186 1103 14-15 0.4483470387070838 1103 16-17 0.4341968974302901 1103 18-19 0.41173944217599256 1103 20-21 0.4150584312522696 1103 22-23 0.4258043321266243 1103 24-25 0.3408882283478505 1103 26-27 0.3270180997146923 1103 28-29 0.3607373855014444 1103 30-31 0.41355746825851014 1103 32-33 0.39671119697740664 1103 34-35 0.46149525227060195 1103 36-37 0.12907377671204756 1103 38-39 0.27646640111512255 1103 40-41 0.29316664825919503 1103 42-43 0.4208715523989923 1103 44-45 0.37744478835963946 1103 46-47 0.31853654035990075 1103 48-49 0.19246718099509508 1103 50-51 0.1432987859817274 1103 52-53 0.08548137340444839 1103 54-55 -0.001125167747751732 1103 56-57 0.7539440729057318 1103 58-59 -6.293996235899893 1103 60-61 -8.636931633927997 1103 62-63 -2.3981265910590466 1103 64-65 -0.16768104430796882 1103 66-67 -1.7449239846528677 1103 68-69 -4.473811045001067 1103 70-71 -5.016823743314706 1103 72-73 -7.751789163460199 1103 74-75 -8.352469100548715 1103 76-77 -9.000789451848025 1103 78-79 -12.224170018460331 1103 80-81 -8.481202688514287 1103 82-83 -4.638699809350605 1103 84-85 -3.6007673673894303 1103 86-87 -3.3322535933734976 1103 88-89 -3.311852724972727 1103 90-91 -8.772930676042554 1103 92-93 -14.804873177748679 1103 94-95 -12.929571882108217 1103 96-97 -12.788566974166404 1103 98-99 -12.359580210087525 1103 100-101 -11.969533814504885 1104 1 0.2118035570904233 1104 2 0.14424128185756757 1104 3 0.17144086931694957 1104 4 0.19061594972003348 1104 5 0.07704041102202552 1104 6 0.1913599933920338 1104 7 0.1220151876938047 1104 8 0.23647542286980894 1104 9 0.152608168769639 1104 10-11 0.2860276616239119 1104 12-13 0.15247919015361333 1104 14-15 0.21426467076905453 1104 16-17 0.2785220019139487 1104 18-19 0.36011904004949713 1104 20-21 0.23403241689876353 1104 22-23 0.24254970575605483 1104 24-25 0.29466123920293796 1104 26-27 0.41461205656872835 1104 28-29 0.233583166250817 1104 30-31 0.37014672305578245 1104 32-33 0.35279551556637045 1104 34-35 0.21782401973225518 1104 36-37 0.26342744668287565 1104 38-39 0.3751629463868156 1104 40-41 0.4654014653756633 1104 42-43 0.41629191200102156 1104 44-45 0.2330166772232687 1104 46-47 0.37288698594878156 1104 48-49 0.3531883510652136 1104 50-51 0.2599224441999155 1104 52-53 0.4000518747157287 1104 54-55 0.29070543003463456 1104 56-57 0.8038972926647148 1104 58-59 -6.785730252428355 1104 60-61 -8.614636167722754 1104 62-63 -2.2017839280466767 1104 64-65 0.08272317168824017 1104 66-67 -1.7208741342394909 1104 68-69 -4.275113848844823 1104 70-71 -4.716381442227528 1104 72-73 -7.883060895076799 1104 74-75 -8.725408174750555 1104 76-77 -9.238101125078611 1104 78-79 -12.53649546874727 1104 80-81 -8.722776904540535 1104 82-83 -4.768395148650043 1104 84-85 -3.820452836608766 1104 86-87 -3.6128849714162605 1104 88-89 -3.679141119901434 1104 90-91 -9.102508488956971 1104 92-93 -14.951312605597792 1104 94-95 -12.412515035186583 1104 96-97 -12.30552852491387 1104 98-99 -11.816564167952931 1104 100-101 -11.409708261578459 1105 1 0.16208674089641661 1105 2 0.2328575785988427 1105 3 0.14243272034824628 1105 4 0.1542509359944404 1105 5 0.13281429724214178 1105 6 0.10477944366392222 1105 7 0.126165426953051 1105 8 0.2388796444117176 1105 9 0.23466435631753058 1105 10-11 0.2770694776683982 1105 12-13 0.2630700584975685 1105 14-15 0.33862067098967685 1105 16-17 0.22260208814164173 1105 18-19 0.2616418133702112 1105 20-21 0.2614811112230626 1105 22-23 0.24328216932558178 1105 24-25 0.37094726182441207 1105 26-27 0.38796410490833466 1105 28-29 0.32452975704739373 1105 30-31 0.25841986335586853 1105 32-33 0.34498046652171155 1105 34-35 0.3349103730172587 1105 36-37 0.13283728272666195 1105 38-39 0.2928338085906361 1105 40-41 0.22891158895583885 1105 42-43 0.1377985973716207 1105 44-45 0.16997164890489103 1105 46-47 0.0474987254342949 1105 48-49 0.12375248590097954 1105 50-51 0.10875817765693085 1105 52-53 0.23526036351829305 1105 54-55 0.03552219951311031 1105 56-57 0.7477898190443639 1105 58-59 -6.439212685596246 1105 60-61 -8.61265441269419 1105 62-63 -2.53944946747146 1105 64-65 -0.40220791460391325 1105 66-67 -1.5519056369842765 1105 68-69 -3.9462641206653934 1105 70-71 -4.506522572291395 1105 72-73 -5.875494541419663 1105 74-75 -6.4575153971835775 1105 76-77 -8.426084436405365 1105 78-79 -11.890330693848991 1105 80-81 -7.735859079344319 1105 82-83 -3.586526554176036 1105 84-85 -2.6419716161839126 1105 86-87 -2.366557921531655 1105 88-89 -2.4826584734236725 1105 90-91 -8.03764899769769 1105 92-93 -11.314816881430714 1105 94-95 -11.604079759448688 1105 96-97 -12.178238849443908 1105 98-99 -11.600284405079083 1105 100-101 -11.151280361976305 1106 1 0.06423519937374778 1106 2 0.1572041154782795 1106 3 0.12622595086159905 1106 4 0.1122326883776168 1106 5 0.09138987939043375 1106 6 0.0455107780869497 1106 7 0.043871914461213635 1106 8 0.13270671579665105 1106 9 0.10799454466119585 1106 10-11 0.18835167337770287 1106 12-13 0.2736933777210453 1106 14-15 0.3258118890089463 1106 16-17 0.10820939174699618 1106 18-19 0.119925781762376 1106 20-21 0.05840315979933308 1106 22-23 0.1306094035431684 1106 24-25 0.14970146948559915 1106 26-27 0.19277904724692263 1106 28-29 0.1980393548490298 1106 30-31 0.21819113442784044 1106 32-33 0.14829883352611972 1106 34-35 0.08165898669498972 1106 36-37 0.14905035485547558 1106 38-39 0.1291224191809377 1106 40-41 0.10221181816480396 1106 42-43 0.1410056061859919 1106 44-45 0.13097936023075363 1106 46-47 0.13235741328981732 1106 48-49 0.1085505129840314 1106 50-51 0.23172566189428068 1106 52-53 0.2822832543693039 1106 54-55 0.13376942609153986 1106 56-57 0.9534595378014785 1106 58-59 -6.405462554794834 1106 60-61 -8.846000759976338 1106 62-63 -2.5391714605154156 1106 64-65 -0.25378906621578423 1106 66-67 -1.521418030050885 1106 68-69 -3.8348511926167017 1106 70-71 -4.587791344236244 1106 72-73 -5.911579757976723 1106 74-75 -6.427331439462737 1106 76-77 -8.399574228369826 1106 78-79 -11.851723988735632 1106 80-81 -7.79337768249923 1106 82-83 -3.6865523345726423 1106 84-85 -2.5797067781238283 1106 86-87 -2.401971172828187 1106 88-89 -2.503135412487655 1106 90-91 -8.026373916203667 1106 92-93 -11.43159145854069 1106 94-95 -11.734343890231447 1106 96-97 -12.354659387285487 1106 98-99 -11.873038583730843 1106 100-101 -11.629882373779687 1107 1 0.057939046465861566 1107 2 0.0688892045250391 1107 3 0.06852715528447106 1107 4 0.02736205417825488 1107 5 -0.05598336520725411 1107 6 0.04213162278031035 1107 7 0.07818171150562137 1107 8 0.07088992629422819 1107 9 0.013381605575148114 1107 10-11 0.050012921496723095 1107 12-13 0.10208898812635425 1107 14-15 0.15896861024977227 1107 16-17 0.1958256109423786 1107 18-19 0.15623934187177468 1107 20-21 0.07815694958627972 1107 22-23 0.05073181140696903 1107 24-25 0.2894917306925038 1107 26-27 0.2485693752800202 1107 28-29 0.13151157415897075 1107 30-31 0.35527169562469396 1107 32-33 0.2956274989131984 1107 34-35 0.07333290281072635 1107 36-37 0.13986021847037478 1107 38-39 0.045222128640411086 1107 40-41 -0.06773487260020516 1107 42-43 0.07165689055311475 1107 44-45 0.07106208833523908 1107 46-47 -0.14136627027245652 1107 48-49 -0.0600722689871418 1107 50-51 0.07271272574040921 1107 52-53 0.004317215374513239 1107 54-55 -0.06635952119298594 1107 56-57 0.7237207890231403 1107 58-59 -6.85867569302977 1107 60-61 -8.97969278974195 1107 62-63 -2.6116384194785063 1107 64-65 -0.18443958344543532 1107 66-67 -1.5391562087608435 1107 68-69 -4.004691592223722 1107 70-71 -4.853492900826357 1107 72-73 -6.155831810026829 1107 74-75 -6.566458211997784 1107 76-77 -9.270245761756586 1107 78-79 -12.348817986771419 1107 80-81 -8.311462948294462 1107 82-83 -4.2366168337861545 1107 84-85 -3.27860054884826 1107 86-87 -3.0535388693854237 1107 88-89 -3.0628734299282705 1107 90-91 -8.269748789178486 1107 92-93 -11.46220459060443 1107 94-95 -12.250427268186609 1107 96-97 -12.66416602754217 1107 98-99 -11.936354189369677 1107 100-101 -11.460372610421137 1108 1 -0.010721550601807905 1108 2 -0.019868706251095603 1108 3 -0.043642314708570495 1108 4 -0.005197918260293477 1108 5 -0.1551333426932473 1108 6 -0.06008830265431442 1108 7 -0.03658383566708068 1108 8 -0.05651877164886088 1108 9 -0.006337941459491958 1108 10-11 0.01106907225381093 1108 12-13 -0.056272194812137855 1108 14-15 -0.04734075817374617 1108 16-17 -0.019837282490691166 1108 18-19 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1.4259288299723138 2312 86-87 1.0957308809326989 2312 88-89 1.2653950505238143 2312 90-91 2.743692197117742 2312 92-93 4.7437848200190516 2312 94-95 8.839911916524617 2312 96-97 9.330537238618746 2312 98-99 9.764377937861228 2312 100-101 8.80042070299118 2313 1 -0.32666434721197746 2313 2 -0.2829176276686596 2313 3 -0.349196532736304 2313 4 -0.3340572210337953 2313 5 -0.3487966708904793 2313 6 -0.2852243292336638 2313 7 -0.2970747351277865 2313 8 -0.48101198235764286 2313 9 -0.3507199233547169 2313 10-11 -0.43931433009596077 2313 12-13 -0.40199036700012414 2313 14-15 -0.5538050301305972 2313 16-17 -0.5804292990546358 2313 18-19 -0.4779385138665617 2313 20-21 -0.5531720423989199 2313 22-23 -0.458097717700376 2313 24-25 -0.5361854956788008 2313 26-27 -0.6985749593657573 2313 28-29 -0.6966153232833889 2313 30-31 -0.672193563665175 2313 32-33 -0.5597330238934788 2313 34-35 -0.5195279265058659 2313 36-37 -0.6126787053703779 2313 38-39 -0.8046716437786046 2313 40-41 -1.0156781764385343 2313 42-43 -0.6751713885775885 2313 44-45 -0.7071514930317591 2313 46-47 -0.5944268302106153 2313 48-49 -0.7382823181922049 2313 50-51 -0.7103711523048375 2313 52-53 -0.7192422171909669 2313 54-55 -0.6941628463293696 2313 56-57 0.33497228654304223 2313 58-59 3.983760328132842 2313 60-61 3.620119027762623 2313 62-63 1.013935702793848 2313 64-65 1.0397454942504503 2313 66-67 1.6777671380706032 2313 68-69 0.25840995538865386 2313 70-71 2.714289067866428 2313 72-73 8.160348133481932 2313 74-75 9.503273670638691 2313 76-77 8.506849204358016 2313 78-79 6.061272728488454 2313 80-81 3.1857821843280014 2313 82-83 1.6155475933146946 2313 84-85 1.192383543578785 2313 86-87 1.1414172033642664 2313 88-89 1.1906315110116452 2313 90-91 2.6942310407172663 2313 92-93 4.624280686410479 2313 94-95 8.652108561363516 2313 96-97 9.359546601600677 2313 98-99 9.781081029028856 2313 100-101 8.849311001281587 2314 1 -0.3846666637547713 2314 2 -0.3303063443607499 2314 3 -0.33917043613119446 2314 4 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1.0419112310205954 2314 66-67 1.5379726805310874 2314 68-69 -0.28542825884489886 2314 70-71 2.703415757096735 2314 72-73 8.481795260218327 2314 74-75 9.79226187696111 2314 76-77 8.726044903033 2314 78-79 6.372163877347628 2314 80-81 3.3587990375825214 2314 82-83 1.714964958460257 2314 84-85 1.2467621416241244 2314 86-87 1.2106502897619826 2314 88-89 1.3693352536887744 2314 90-91 2.643595969233054 2314 92-93 4.747031717900857 2314 94-95 8.615676640742631 2314 96-97 9.36007616592024 2314 98-99 9.818771504832954 2314 100-101 9.147011092158222 2315 1 -0.7728288095704485 2315 2 -0.5785356340250125 2315 3 -0.5457049871824395 2315 4 -0.4768594581721075 2315 5 -0.6326930821994878 2315 6 -0.5878434798163283 2315 7 -0.6412507163070202 2315 8 -0.7542873146591731 2315 9 -1.0017973686470398 2315 10-11 -0.8613187797500004 2315 12-13 -0.7469319245549144 2315 14-15 -0.9496126718243048 2315 16-17 -0.9813500081025026 2315 18-19 -0.8590593745813138 2315 20-21 -0.9803900413064284 2315 22-23 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0.7955342259110019 2315 90-91 2.156952126759773 2315 92-93 4.20270493366268 2315 94-95 8.302977542277905 2315 96-97 9.068064000875658 2315 98-99 9.19119201691206 2315 100-101 8.359751807743882 2316 1 -0.5154187679884039 2316 2 -0.4531307620602618 2316 3 -0.6156183420358268 2316 4 -0.3865012279277593 2316 5 -0.6357743266158877 2316 6 -0.46946235137421866 2316 7 -0.6689637665091865 2316 8 -0.7160382574562476 2316 9 -0.7661385780126224 2316 10-11 -0.7138379888894306 2316 12-13 -0.6883995098584279 2316 14-15 -0.8117647195761535 2316 16-17 -0.913803079775974 2316 18-19 -0.9789914404409288 2316 20-21 -0.7264000138940787 2316 22-23 -0.9577302533925476 2316 24-25 -2.244224853321647 2316 26-27 -1.5598381478456673 2316 28-29 -1.2255381766603008 2316 30-31 -0.8384367211385353 2316 32-33 -0.7647631467992042 2316 34-35 -0.912608800230565 2316 36-37 -0.9498598033934584 2316 38-39 -1.1207376773439037 2316 40-41 -1.0026571945576066 2316 42-43 -1.0520614869551466 2316 44-45 -0.9854415973172621 2316 46-47 -0.9815243129145799 2316 48-49 -1.1812077644461567 2316 50-51 -1.2639890459576648 2316 52-53 -1.1883666844568452 2316 54-55 -0.9481333325536596 2316 56-57 0.1753254062462588 2316 58-59 3.9171793224978764 2316 60-61 3.8270315457920567 2316 62-63 1.1503396379978987 2316 64-65 0.7476763697067916 2316 66-67 5.827707727521041 2316 68-69 8.69447180245831 2316 70-71 5.574733292234075 2316 72-73 9.220754058035375 2316 74-75 10.042040518482494 2316 76-77 8.572801471686642 2316 78-79 6.101592195795082 2316 80-81 2.9245474660557313 2316 82-83 1.5805681998370673 2316 84-85 1.42729426986957 2316 86-87 1.21343562394512 2316 88-89 1.432577918264979 2316 90-91 2.9106914554146144 2316 92-93 4.529976688765512 2316 94-95 8.779077920493538 2316 96-97 9.43020632099342 2316 98-99 9.867537766536602 2316 100-101 8.67227418456657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 47.0 5 110.0 6 255.0 7 681.0 8 1480.0 9 2587.0 10 4242.0 11 6627.0 12 9162.0 13 12733.0 14 16651.0 15 22921.0 16 30361.0 17 39076.0 18 41400.0 19 40055.0 20 41245.0 21 41030.0 22 38995.0 23 38310.0 24 39656.0 25 42924.0 26 56512.0 27 81367.0 28 113726.0 29 150534.0 30 179738.0 31 194861.0 32 200659.0 33 209857.0 34 214387.0 35 210789.0 36 181046.0 37 117606.0 38 40593.0 39 1957.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.62571992172302 19.85537820052886 14.682751709059822 22.8361501686883 2 15.469954906473527 21.45987352691111 39.64591825411198 23.424253312503378 3 19.025739692324954 25.806685818665443 30.361377095815545 24.806197393194054 4 12.738134377027723 17.4643357895297 37.31155193302227 32.4859779004203 5 13.041414354059086 38.55014674377423 34.124470091926405 14.28396881024028 6 30.999209225944814 37.04448506253797 17.62493879441813 14.331366917099075 7 27.72021719249454 33.7306313110792 20.85872133962047 17.690430156805785 8 26.257908269233226 35.285988838393486 19.8306824964741 18.62542039589919 9 26.737999900173627 16.48587229896432 20.161627978445754 36.6144998224163 10-11 24.695560490040254 27.30424645729803 27.298388876536634 20.701804176125076 12-13 25.80594329944324 24.59348595768764 27.970525643622313 21.630045099246807 14-15 22.550286651029904 25.726837120008977 26.715904862713586 25.006971366247534 16-17 21.65937425362252 29.255645862217328 27.158178193381843 21.926801690778312 18-19 21.493619813178412 27.463813088483985 29.903417004770056 21.13915009356755 20-21 22.605476673009626 26.471913106501955 29.7943576647063 21.12825255578211 22-23 22.011220979709258 26.744001857925614 29.667388414117763 21.577388748247365 24-25 21.76427444993398 26.776548556663226 29.963505621833765 21.495671371569035 26-27 21.663826562665648 27.05303218743284 29.817400081758695 21.46574116814282 28-29 21.447032847358937 27.28181942873172 29.88529051726005 21.38585720664929 30-31 21.553197357041004 27.012793388518396 29.823180490469266 21.610828763971334 32-33 21.39077845568658 27.081204197584896 29.70575696306024 21.822260383668286 34-35 21.884777770673445 27.01563618288208 29.575898704100556 21.523687342343923 36-37 21.877186232165865 26.81236905816783 29.637979036928304 21.672465672738 38-39 21.53851486063615 27.150802282900038 29.72609985872163 21.584582997742185 40-41 21.754848607259408 26.85736225367037 29.782948928649972 21.60484021042025 42-43 21.516414707990982 26.948241447073208 29.532816521474516 22.002527323461297 44-45 21.60476965431642 27.132298571315395 29.289354847228456 21.973576927139725 46-47 21.664135158989822 27.234896265953967 28.967049962099594 22.133918612956617 48-49 21.709991386291307 27.275953310604145 28.85945212686394 22.154603176240606 50-51 21.591260045820597 27.45866913958984 28.76933217664346 22.1807386379461 52-53 21.835640261544697 27.46239959730616 28.57199441757412 22.129965723575022 54-55 21.35863828389742 27.272258436100916 28.752767612930878 22.616335667070786 56-57 21.584127368419747 27.553055370442518 28.56136252743816 22.301454733699575 58-59 20.195233534377266 27.08221748064182 30.2011962910407 22.52135269394022 60-61 21.680953835026855 27.21227678626843 28.763632391530475 22.343136987174237 62-63 21.337501582997106 27.240956703636954 28.76374835564242 22.65779335772352 64-65 20.651398997116917 27.195748073126204 29.216094869104758 22.93675806065212 66-67 20.84577993536306 27.292933327338748 29.02451749703843 22.83676924025976 68-69 21.491893476609516 26.64169331841485 29.093541175309262 22.77287202966637 70-71 20.631917105477502 26.99350987764295 29.29528318129584 23.079289835583708 72-73 20.510688873513157 26.921346143952523 29.395058371192277 23.17290661134204 74-75 20.969979526498182 26.962829192110217 28.746706325133115 23.32048495625849 76-77 19.460461551228818 27.240605231870298 29.537477110723838 23.76145610617705 78-79 20.353195918389325 27.370581737621038 28.532777179295067 23.743445164694574 80-81 20.701767919062984 27.423205783666898 28.129622647580284 23.745403649689838 82-83 21.092701169683156 27.258788298919622 27.749939309963047 23.898571221434175 84-85 21.02731241406991 27.04488350633134 27.68971239690967 24.238091682689074 86-87 20.972372791580657 26.935558789702874 27.9089587530712 24.18310966564527 88-89 20.914805801563684 26.935781307589675 27.696895708434322 24.452517182412322 90-91 20.709164005415516 27.006872745687737 27.8477247594539 24.436238489442843 92-93 20.485702722150798 26.78519447136526 28.126012973916847 24.603089832567097 94-95 19.322203660933923 26.879267788154603 28.64708256993393 25.151445980977545 96-97 19.099770229175782 26.68275027638267 28.985636452655832 25.231843041785716 98-99 19.31409114911721 26.61698980365243 28.471668113134314 25.597250934096046 100-101 19.11858483300069 26.622332276043693 28.73309509809605 25.525987792859567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 574.0 1 432.5 2 315.5 3 379.5 4 443.5 5 509.0 6 692.5 7 1133.5 8 1624.0 9 1958.0 10 2184.5 11 2404.5 12 2736.0 13 3074.0 14 3364.5 15 3646.0 16 3883.0 17 4161.5 18 4480.0 19 4738.0 20 5211.0 21 6105.0 22 7087.0 23 8270.5 24 9858.5 25 11931.0 26 14511.0 27 17745.0 28 21152.0 29 24861.5 30 29239.5 31 33930.5 32 39008.0 33 44273.0 34 49611.0 35 54812.0 36 59944.0 37 64861.0 38 69043.0 39 72662.5 40 76558.5 41 81127.0 42 85339.0 43 89024.5 44 92241.5 45 94859.0 46 97185.5 47 99173.0 48 101300.0 49 101123.5 50 97540.0 51 92707.0 52 87288.0 53 82011.5 54 76096.0 55 68390.5 56 60254.0 57 52192.5 58 44450.0 59 37266.0 60 30350.0 61 23917.0 62 18227.5 63 13580.5 64 10018.0 65 7325.5 66 5335.0 67 3860.5 68 2632.0 69 1763.5 70 1268.0 71 962.5 72 723.5 73 546.5 74 397.5 75 255.5 76 152.5 77 86.5 78 45.5 79 24.5 80 13.0 81 10.0 82 8.0 83 5.5 84 5.0 85 2.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020955288921046 2 0.005610077348941448 3 0.0038363028930261376 4 0.0 5 0.06678467079364857 6 8.250113748443307E-5 7 0.0 8 2.475034124532992E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.887539811955158E-4 16-17 0.0 18-19 0.001010638934184305 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.250113748443307E-5 28-29 0.0017118986028019862 30-31 0.007569479364196734 32-33 2.2687812808219092E-4 34-35 9.900136498131968E-4 36-37 0.027287251222976237 38-39 0.025781605463885335 40-41 0.027967885607222808 42-43 0.028731021128953815 44-45 0.029741660063138123 46-47 0.03102042769414683 48-49 0.030793549566064643 50-51 0.025843481316998658 52-53 0.021924677286488087 54-55 0.017098360743648754 56-57 0.15534964188318748 58-59 0.7261750121379799 60-61 0.30339793309900265 62-63 4.125056874221653E-4 64-65 0.13154806371892852 66-67 1.3679307353450136 68-69 2.6328588005407125 70-71 2.1286737240889706 72-73 1.778373894330068 74-75 1.505171377550368 76-77 1.0829099306206684 78-79 0.4030180566114555 80-81 1.237517062266496E-4 82-83 2.2687812808219092E-4 84-85 0.0 86-87 0.0031762937931506733 88-89 2.062528437110827E-5 90-91 0.2159054767967613 92-93 0.16687917584663697 94-95 1.2391052091630712 96-97 1.2140661139365458 98-99 0.946989306615065 100-101 0.38070149892191635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2424209.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.66070502749675 #Duplication Level Percentage of deduplicated Percentage of total 1 70.63621792149131 35.0784438245716 2 14.931621127505526 14.830296647907806 3 5.855989749429323 8.724377387713627 4 2.927685437368243 5.8156369167376845 5 1.6658299037410846 4.136314373783465 6 1.0236620376097894 3.050146709855162 7 0.6526651566748009 2.2688268279146566 8 0.4366718090394079 1.734834392202354 9 0.31251548198732026 1.3967765250748456 >10 1.4078731163560043 12.140831659020666 >50 0.09116013672602281 3.135169733902548 >100 0.05353479711959906 4.904416246265645 >500 0.0037418113239342144 1.218985684077195 >1k 5.820595392780262E-4 0.4296181252414743 >5k 1.663027255080075E-4 0.6592515733833273 >10k+ 8.315136275400375E-5 0.4760733723480351 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11529 0.4755778070290144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9369 0.38647657854582673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6596 0.27208875142366024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.125056874221653E-5 0.0 10-11 0.0 0.0 0.0 6.18758531133248E-5 0.0 12-13 0.0 0.0 0.0 8.250113748443306E-5 0.0 14-15 0.0 0.0 0.0 8.250113748443306E-5 0.0 16-17 0.0 0.0 0.0 1.6500227496886612E-4 0.0 18-19 0.0 0.0 0.0 2.681286968244075E-4 0.0 20-21 0.0 0.0 0.0 3.0937926556662396E-4 0.0 22-23 0.0 0.0 0.0 0.001010638934184305 0.0 24-25 0.0 0.0 0.0 0.0017325238871730942 0.0 26-27 0.0 0.0 0.0 0.0033619213524906473 0.0 28-29 0.0 0.0 0.0 0.010477644460523 0.0 30-31 0.0 0.0 0.0 0.03504235814651294 0.0 32-33 0.0 0.0 0.0 0.09089562822347413 0.0 34-35 0.0 0.0 0.0 0.1763461813729757 0.0 36-37 0.0 0.0 0.0 0.3148449659249677 0.0 38-39 0.0 0.0 0.0 0.5245009815572832 0.0 40-41 0.0 0.0 0.0 0.7526578772704828 0.0 42-43 0.0 0.0 0.0 0.9806910212774558 0.0 44-45 0.0 0.0 0.0 1.246097180564877 0.0 46-47 0.0 0.0 0.0 1.5469788289706043 0.0 48-49 0.0 4.125056874221653E-5 0.0 1.845241066261201 0.0 50-51 0.0 6.18758531133248E-5 0.0 2.1402857591899047 2.0625284371108265E-5 52-53 0.0 8.250113748443306E-5 0.0 2.420191493390215 4.125056874221653E-5 54-55 0.0 8.250113748443306E-5 0.0 2.718123726130874 4.125056874221653E-5 56-57 0.0 8.250113748443306E-5 0.0 2.981632359256153 4.125056874221653E-5 58-59 0.0 8.250113748443306E-5 0.0 3.167940552980374 6.18758531133248E-5 60-61 0.0 8.250113748443306E-5 0.0 3.3110800265158655 8.250113748443306E-5 62-63 4.125056874221653E-5 1.237517062266496E-4 0.0 3.4312635585463136 8.250113748443306E-5 64-65 4.125056874221653E-5 1.237517062266496E-4 0.0 3.5541490028293765 8.250113748443306E-5 66-67 4.125056874221653E-5 1.237517062266496E-4 0.0 3.660162964496873 8.250113748443306E-5 68-69 4.125056874221653E-5 1.237517062266496E-4 0.0 3.778015839393386 8.250113748443306E-5 70-71 4.125056874221653E-5 1.237517062266496E-4 0.0 3.994498824152538 8.250113748443306E-5 72-73 4.125056874221653E-5 1.237517062266496E-4 0.0 4.2605031166867215 1.0312642185554133E-4 74-75 4.125056874221653E-5 1.237517062266496E-4 0.0 4.561364139808077 1.237517062266496E-4 76-77 4.125056874221653E-5 1.4437699059775786E-4 0.0 4.987296887355834 1.237517062266496E-4 78-79 4.125056874221653E-5 2.0625284371108266E-4 0.0 5.582851973571586 1.237517062266496E-4 80-81 4.125056874221653E-5 2.2687812808219092E-4 0.0 6.24492360188416 1.237517062266496E-4 82-83 6.18758531133248E-5 2.475034124532992E-4 0.0 6.866136541857571 1.237517062266496E-4 84-85 8.250113748443306E-5 2.475034124532992E-4 0.0 7.370053489612488 1.237517062266496E-4 86-87 8.250113748443306E-5 2.475034124532992E-4 0.0 7.8139508598474805 1.237517062266496E-4 88-89 8.250113748443306E-5 2.475034124532992E-4 0.0 8.27422470587313 1.237517062266496E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8430 0.0 37.667133 1 GTATCAA 13365 0.0 32.654865 1 GTACATG 13245 0.0 27.17989 1 ATCAACG 16175 0.0 26.799137 3 TATCAAC 16295 0.0 26.631277 2 TCAACGC 16305 0.0 26.524874 4 TACATGG 13385 0.0 26.218817 2 CAACGCA 16630 0.0 26.006498 5 AACGCAG 16985 0.0 25.47441 6 ACATGGG 13425 0.0 24.945583 3 ACGCAGA 19250 0.0 22.477032 7 CGCAGAG 19400 0.0 22.230814 8 CATGGGG 4545 0.0 21.584114 4 GAGTACT 9975 0.0 21.547276 12-13 CATGGGA 9025 0.0 21.164722 4 GCAGAGT 20280 0.0 20.84781 9 AGTACTT 10305 0.0 20.262648 12-13 GTACTTT 10670 0.0 20.18795 14-15 AGAGTAC 17850 0.0 18.906485 10-11 ATGGGAG 3550 0.0 18.732492 5 >>END_MODULE