FastQCFastQC Report
Fri 27 May 2016
SRR939848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499562
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111130.44459789355095014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.3111345107662863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.21959847365258392No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26110.10445830109435174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85100.035.7711751
GTATCAA141750.035.3164521
TATCAAC170200.029.4130842
ATCAACG171350.029.1075953
TCAACGC172000.028.915534
CAACGCA175550.028.3307935
AACGCAG181150.027.4566356
GTACATG134200.025.3129751
TACATGG134300.024.9087582
ACGCAGA200900.024.6403277
CGCAGAG202650.024.3346638
ACATGGG137150.023.3976973
GAGTACT93050.022.7062312-13
GCAGAGT204200.022.4677499
ATGGGAG34400.022.158545
AGTACTT97800.021.1703912-13
CATGGGA93300.020.7778284
GTACTTT103650.020.4342314-15
AGAGTAC173850.019.79813210-11
CATGGGG47200.018.9407084
Produced by FastQC (version 0.11.5)