FastQCFastQC Report
Fri 27 May 2016
SRR939848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499562
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119140.4766435079425916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95980.38398727457050474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.2645663520248748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.035.127081
GTATCAA132150.032.5403861
GTACATG134900.026.7401181
ATCAACG160850.026.3986153
TCAACGC162050.026.2324094
TACATGG135100.026.2029112
TATCAAC162350.026.1014882
CAACGCA166100.025.5927875
AACGCAG168450.025.2357486
ACATGGG135050.025.0480633
ACGCAGA193250.021.9235637
CGCAGAG194650.021.7415078
CATGGGG46100.021.6121314
GAGTACT97150.021.19464112-13
CATGGGA93150.020.678664
TGCACCG5150.020.2672425
GCAGAGT206750.020.101939
GTACTTT104000.019.7986514-15
ATTCGCG3250.019.70800480-81
AGTACTT100150.019.65967612-13
Produced by FastQC (version 0.11.5)