##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939848_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2499562 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42819942053848 31.0 31.0 34.0 30.0 34.0 2 31.654360643984827 31.0 31.0 34.0 30.0 34.0 3 31.69234809938701 31.0 31.0 34.0 30.0 34.0 4 35.49971595023448 37.0 35.0 37.0 33.0 37.0 5 35.24216762776838 37.0 35.0 37.0 33.0 37.0 6 35.26986287997657 37.0 35.0 37.0 32.0 37.0 7 35.17605484480881 37.0 35.0 37.0 32.0 37.0 8 35.144073241631936 37.0 35.0 37.0 32.0 37.0 9 36.721365983320275 39.0 35.0 39.0 32.0 39.0 10-11 36.58979793259779 39.0 35.0 39.0 32.0 39.0 12-13 36.50310334370582 38.0 35.0 39.0 32.0 39.0 14-15 37.52242512888258 39.0 36.0 41.0 32.0 41.0 16-17 37.45556061421961 39.0 36.0 41.0 32.0 41.0 18-19 37.467301871287845 39.0 36.0 41.0 32.0 41.0 20-21 37.4029966050052 39.0 36.0 41.0 32.0 41.0 22-23 37.2727169800149 39.0 36.0 40.5 31.0 41.0 24-25 37.121232439923475 39.0 36.0 40.0 31.0 41.0 26-27 36.83779118101491 39.0 36.0 40.0 30.5 41.0 28-29 36.607833292392826 38.5 35.0 40.0 30.0 41.0 30-31 36.33707585568992 38.0 35.0 40.0 30.0 41.0 32-33 36.29889376618783 38.0 35.0 40.0 30.0 41.0 34-35 36.136362890778464 38.0 35.0 40.0 30.0 41.0 36-37 36.15685888167607 38.0 35.0 40.0 30.0 41.0 38-39 36.06439368177305 38.0 35.0 40.0 30.0 41.0 40-41 35.96958747172505 38.0 35.0 40.0 29.5 41.0 42-43 35.73162398052139 38.0 35.0 40.0 28.5 41.0 44-45 35.46043446811881 38.0 34.0 40.0 27.0 41.0 46-47 35.13115337807184 38.0 34.0 40.0 26.5 41.0 48-49 34.804997835620796 38.0 33.5 40.0 25.5 41.0 50-51 34.58138285827677 38.0 33.0 40.0 24.0 41.0 52-53 34.334774052413984 38.0 33.0 40.0 24.0 41.0 54-55 34.08587824586868 38.0 33.0 40.0 23.5 41.0 56-57 33.73588752749482 37.0 32.5 40.0 23.0 41.0 58-59 33.388728305199066 37.0 32.0 40.0 22.0 41.0 60-61 33.08443319269536 36.0 32.0 39.0 22.0 41.0 62-63 32.806910970802086 36.0 31.0 39.0 20.5 40.5 64-65 32.3810621620908 35.5 31.0 39.0 19.5 40.0 66-67 31.533960949958434 35.0 30.0 38.5 13.5 40.0 68-69 29.756014453732295 34.0 27.0 37.5 7.5 40.0 70-71 29.110754404171608 34.0 26.0 37.0 7.5 39.0 72-73 29.630296828004266 34.0 27.5 36.5 7.5 39.0 74-75 29.68292884913437 34.0 28.0 36.0 8.0 39.0 76-77 29.152119251292824 32.5 27.5 35.0 7.0 38.0 78-79 29.662499269872082 34.0 29.0 36.0 7.0 37.5 80-81 29.464748423923872 34.0 29.0 35.0 4.0 37.0 82-83 29.023646342839264 34.0 29.0 35.0 2.0 37.0 84-85 28.66572643527146 33.5 28.5 35.0 2.0 36.0 86-87 28.277597835140718 33.0 27.5 35.0 2.0 36.0 88-89 27.832701489300923 33.0 26.5 35.0 2.0 35.5 90-91 27.350984492483082 33.0 26.0 35.0 2.0 35.0 92-93 26.87662898539824 32.5 25.5 35.0 2.0 35.0 94-95 26.103881400021287 32.0 24.0 34.0 2.0 35.0 96-97 25.59225436296439 32.0 23.0 34.0 2.0 35.0 98-99 24.945638075790878 32.0 19.0 34.0 2.0 35.0 100-101 23.480718021797422 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2703801700273374 1101 2 0.24514261367616896 1101 3 0.26558945853704685 1101 4 0.2547248141066447 1101 5 0.13986205550342845 1101 6 0.20956932748610058 1101 7 0.16741697821588986 1101 8 0.2595035371479355 1101 9 0.3769451641626773 1101 10-11 0.29794253601268395 1101 12-13 0.31178027670892305 1101 14-15 0.2847412480533791 1101 16-17 0.377501706668653 1101 18-19 0.3254717277091643 1101 20-21 0.29579954048222845 1101 22-23 0.22249863033112405 1101 24-25 0.37107859621801254 1101 26-27 0.3647204832235502 1101 28-29 0.2675732139702873 1101 30-31 0.04180545467080776 1101 32-33 0.07590407235621655 1101 34-35 0.11129235805111648 1101 36-37 0.3496081994744955 1101 38-39 0.3760164145306675 1101 40-41 0.3787077948964068 1101 42-43 0.2897382446809402 1101 44-45 0.20284715057695024 1101 46-47 0.08043951013057438 1101 48-49 0.13554673278631668 1101 50-51 0.18831016106022958 1101 52-53 0.12441350881768898 1101 54-55 -0.1278088147353742 1101 56-57 -0.1931455019965611 1101 58-59 -0.19161836616851957 1101 60-61 -0.25452125978130624 1101 62-63 -0.24594884844061937 1101 64-65 -0.3272973379068773 1101 66-67 -0.34365690438016827 1101 68-69 -9.00000907624408 1101 70-71 -6.487114132879004 1101 72-73 -1.8291832768927634 1101 74-75 -0.7581184238436656 1101 76-77 -0.6329108862357913 1101 78-79 -0.8159675466378218 1101 80-81 -0.7807616698859121 1101 82-83 -0.8095392856920292 1101 84-85 -0.8299130799223704 1101 86-87 -0.7586159097731695 1101 88-89 -0.7596059174613892 1101 90-91 -1.1320333590347538 1101 92-93 -1.3995857076621832 1101 94-95 -1.1277748962078071 1101 96-97 -1.4443054620499538 1101 98-99 -1.1178307755648156 1101 100-101 -1.0740170582482342 1102 1 0.19533142397268577 1102 2 0.07866191598100514 1102 3 0.2285562630756317 1102 4 0.18249250676507245 1102 5 0.2350276646485696 1102 6 0.2572055863379177 1102 7 0.2218483025651281 1102 8 0.1777889362708578 1102 9 0.3393951260012784 1102 10-11 0.36782303206840083 1102 12-13 0.21308084410308226 1102 14-15 0.31166715971434655 1102 16-17 0.3089989858104403 1102 18-19 0.1817340558235685 1102 20-21 0.2521799636135569 1102 22-23 0.2372546664166606 1102 24-25 0.165010402717769 1102 26-27 0.12682026643281574 1102 28-29 0.03214693910342703 1102 30-31 0.04667634311633151 1102 32-33 -0.1724685811425246 1102 34-35 -0.18361472354347086 1102 36-37 -0.08235052107786345 1102 38-39 0.08133872613442605 1102 40-41 -0.04072687123029084 1102 42-43 -0.1713776611885507 1102 44-45 0.01954499300647683 1102 46-47 -0.03161092522090314 1102 48-49 -0.1035220441089777 1102 50-51 -0.10325868309541875 1102 52-53 -0.14315684196981948 1102 54-55 -0.4405805996551848 1102 56-57 -0.3929374197095399 1102 58-59 -0.5281603132636548 1102 60-61 -0.4377133227766521 1102 62-63 -0.443122745477055 1102 64-65 -0.44298248724609834 1102 66-67 -0.027167569039882267 1102 68-69 -4.939713534260168 1102 70-71 -0.21447305960203877 1102 72-73 0.025929607216944817 1102 74-75 -0.3079956914025139 1102 76-77 -0.5145528459333875 1102 78-79 -0.44126225317737067 1102 80-81 -0.5295644356315634 1102 82-83 -0.5755099021225298 1102 84-85 -0.5603930639498067 1102 86-87 -0.5440428218433588 1102 88-89 -0.5689755676084438 1102 90-91 -0.7614943133387762 1102 92-93 -0.7768051222564054 1102 94-95 -0.5442612579325612 1102 96-97 -1.2112494065651198 1102 98-99 -1.1126858896879313 1102 100-101 -0.9939037847364958 1103 1 0.21534930138248498 1103 2 0.16825396703078965 1103 3 0.2226867756901818 1103 4 0.17655377348282997 1103 5 0.24474688194082717 1103 6 0.1919219226589135 1103 7 0.15282946391969432 1103 8 0.2609484709521084 1103 9 0.3634273846724909 1103 10-11 0.32235822796247504 1103 12-13 0.30377501626582415 1103 14-15 0.3622190580606741 1103 16-17 0.42580535821111454 1103 18-19 0.3824409794899708 1103 20-21 0.33763187943380046 1103 22-23 0.2679207674896773 1103 24-25 0.2188443001059568 1103 26-27 0.1598186718964456 1103 28-29 0.2935222446830039 1103 30-31 0.22969444519058868 1103 32-33 0.22891269497215916 1103 34-35 0.20984918190458757 1103 36-37 0.1636130415602679 1103 38-39 0.2899899494273086 1103 40-41 0.1410102672420166 1103 42-43 0.10565867256094208 1103 44-45 0.16761255374880335 1103 46-47 0.1539732930066009 1103 48-49 0.09081904442283673 1103 50-51 -0.0626280974734641 1103 52-53 -0.054046605579721074 1103 54-55 -0.08170501965668109 1103 56-57 0.026774014685877034 1103 58-59 -0.20814826618738635 1103 60-61 -0.14595753407613898 1103 62-63 -0.08380989046360554 1103 64-65 -0.22468676383848418 1103 66-67 -0.17839949628826446 1103 68-69 -4.4252676433458795 1103 70-71 0.17855528060630022 1103 72-73 0.3376253075767828 1103 74-75 0.28377743441881975 1103 76-77 0.061292184827177465 1103 78-79 -0.18048468501803683 1103 80-81 -0.15020181590027448 1103 82-83 -0.15984088930229134 1103 84-85 -0.18889464087549968 1103 86-87 -0.22330830924385126 1103 88-89 -0.3379760432679113 1103 90-91 -0.3549353248965197 1103 92-93 -0.4082591390484467 1103 94-95 -0.5312184085716218 1103 96-97 -0.818272359613669 1103 98-99 -0.50014976641177 1103 100-101 -0.5624709007662894 1104 1 0.17961548576138142 1104 2 0.27990596625936703 1104 3 0.21986384540525705 1104 4 0.1810157286223344 1104 5 0.20315154458479867 1104 6 0.18102830707221074 1104 7 0.13756908156980074 1104 8 0.06666701181345758 1104 9 0.22861188899636886 1104 10-11 0.20730299639443928 1104 12-13 0.31169721761601465 1104 14-15 0.32566696944427065 1104 16-17 0.26607207405844235 1104 18-19 0.254840961047222 1104 20-21 0.23752841165183725 1104 22-23 0.2650901527264651 1104 24-25 0.22216647359370967 1104 26-27 0.404526917044187 1104 28-29 0.3309498680523504 1104 30-31 0.2832168365209071 1104 32-33 0.41753664288031445 1104 34-35 0.3731597146471586 1104 36-37 0.37541263234940203 1104 38-39 0.4427412653322449 1104 40-41 0.32528232157077497 1104 42-43 0.42568100216755766 1104 44-45 0.30482387144844125 1104 46-47 0.34632099036507213 1104 48-49 0.4219724647841332 1104 50-51 0.4397327725681279 1104 52-53 0.34069810365696895 1104 54-55 0.36631469028648667 1104 56-57 0.3873795614910378 1104 58-59 0.293732695790311 1104 60-61 0.28880496348837426 1104 62-63 0.46047352697758015 1104 64-65 0.4880409136196633 1104 66-67 0.6541279583695996 1104 68-69 -3.8948566923198022 1104 70-71 0.8342728051334198 1104 72-73 0.9962527705140474 1104 74-75 0.8311916570495583 1104 76-77 0.8418911576851045 1104 78-79 0.4159897259171288 1104 80-81 0.24475483620554428 1104 82-83 0.34435706682478795 1104 84-85 0.37232511884325703 1104 86-87 0.3567252843206212 1104 88-89 0.26719248725656897 1104 90-91 0.3689318714537997 1104 92-93 0.18879532605484783 1104 94-95 0.4745092545091225 1104 96-97 -0.045368276280211006 1104 98-99 0.34221010216878156 1104 100-101 0.2239566249358127 1105 1 0.18464296908257083 1105 2 0.20933699221435376 1105 3 0.20293916102160736 1105 4 0.11077527658113695 1105 5 0.26093088841811607 1105 6 0.17644362118819856 1105 7 0.06082557858115223 1105 8 0.20511257347641276 1105 9 0.22173168143785205 1105 10-11 0.1234384173921299 1105 12-13 0.10642667251425308 1105 14-15 0.08335831006834127 1105 16-17 0.31330692644446145 1105 18-19 0.17523580412591144 1105 20-21 0.1833619357091294 1105 22-23 0.13510280090307702 1105 24-25 0.267904540314035 1105 26-27 0.24346419683749332 1105 28-29 0.407779037881852 1105 30-31 0.25746632188412377 1105 32-33 0.26774562864849116 1105 34-35 0.37852334893017314 1105 36-37 0.17917048222930987 1105 38-39 0.4351583194864048 1105 40-41 0.3920792526952752 1105 42-43 0.33093482713637457 1105 44-45 0.23423757331130446 1105 46-47 0.21580970395746135 1105 48-49 0.4200719800910022 1105 50-51 0.3933032520608535 1105 52-53 0.27935317557897577 1105 54-55 0.36307659495757605 1105 56-57 0.38811122624411354 1105 58-59 0.43772888067556437 1105 60-61 0.3252535818205118 1105 62-63 0.4064458027084683 1105 64-65 0.4109637231868888 1105 66-67 0.9211720041586062 1105 68-69 2.1608551244275382 1105 70-71 2.526450468943043 1105 72-73 1.6887764393562392 1105 74-75 1.144220995479035 1105 76-77 0.7336493559540926 1105 78-79 0.5495292318403777 1105 80-81 0.6313681764557977 1105 82-83 0.5659718597557948 1105 84-85 0.43441037372038593 1105 86-87 0.34288746706722506 1105 88-89 0.4162304597592623 1105 90-91 0.33005986693236977 1105 92-93 0.30993070255028243 1105 94-95 0.6121401070324595 1105 96-97 0.1399133657767102 1105 98-99 0.41003217452436047 1105 100-101 0.32077501568341305 1106 1 0.4693827167166411 1106 2 0.3875449905861892 1106 3 0.38959200522634774 1106 4 0.3518597886397572 1106 5 0.3948705444677785 1106 6 0.39694199302090283 1106 7 0.3179475384875303 1106 8 0.26837450488987 1106 9 0.39553599608118617 1106 10-11 0.3997676633471485 1106 12-13 0.4376397843489954 1106 14-15 0.5405349662197665 1106 16-17 0.49580603604047013 1106 18-19 0.520060012938373 1106 20-21 0.4611857883599839 1106 22-23 0.5098712958659277 1106 24-25 0.5384385283063011 1106 26-27 0.5336614849213461 1106 28-29 0.6117794958039013 1106 30-31 0.729443400341431 1106 32-33 0.7599660417959413 1106 34-35 0.6090533937047695 1106 36-37 0.6314842605950375 1106 38-39 0.7907341564661579 1106 40-41 0.7552464196841839 1106 42-43 0.770510740436464 1106 44-45 0.8621956225633696 1106 46-47 0.7152760975668997 1106 48-49 0.7726859518015985 1106 50-51 0.869804218785184 1106 52-53 0.87889143751039 1106 54-55 0.8203448355192933 1106 56-57 0.8374466032665993 1106 58-59 0.9938230599774869 1106 60-61 0.9968369745141032 1106 62-63 0.919872458859885 1106 64-65 0.9807459558112868 1106 66-67 1.272546406315925 1106 68-69 2.7659183431107586 1106 70-71 3.1200802644045247 1106 72-73 1.9847810694569823 1106 74-75 1.573120964950899 1106 76-77 1.123943952473887 1106 78-79 0.934976850645711 1106 80-81 1.0918189752753769 1106 82-83 1.0580116480940482 1106 84-85 1.185642947242318 1106 86-87 0.9904503041487338 1106 88-89 1.1597807548645847 1106 90-91 1.0774955034440232 1106 92-93 0.9789478237469851 1106 94-95 1.3533689917378666 1106 96-97 1.1982030269143884 1106 98-99 1.4082621785947786 1106 100-101 1.5151807346210333 1107 1 0.14089513425641442 1107 2 0.10152066873156684 1107 3 0.06624324385090574 1107 4 0.022477191971958632 1107 5 0.07375321624165565 1107 6 0.08295677014358915 1107 7 0.0745100893406132 1107 8 0.06336086061733681 1107 9 -0.027612951807711283 1107 10-11 0.015611918357350874 1107 12-13 0.059890353903490734 1107 14-15 0.10359763599080907 1107 16-17 0.05029339005301381 1107 18-19 0.007711789823218851 1107 20-21 0.10293460230968776 1107 22-23 0.21425796078012382 1107 24-25 0.1672379575000349 1107 26-27 0.09744196547512729 1107 28-29 0.21670160453248855 1107 30-31 0.1060261828967981 1107 32-33 0.12355476712046709 1107 34-35 0.23828695173048686 1107 36-37 0.04439468406819458 1107 38-39 0.14718537819184263 1107 40-41 0.06166087072127624 1107 42-43 0.03178729356775989 1107 44-45 0.13851944558838625 1107 46-47 0.2039725531854657 1107 48-49 0.30386342748600015 1107 50-51 0.13709019773044417 1107 52-53 0.08223131133215844 1107 54-55 0.16336669764955047 1107 56-57 0.15495375399199673 1107 58-59 0.2694742923432045 1107 60-61 0.3678654272035331 1107 62-63 0.343012253194388 1107 64-65 0.29858944098409523 1107 66-67 0.7396676995175682 1107 68-69 2.0681543921550336 1107 70-71 2.3994494045650043 1107 72-73 1.3358657265351042 1107 74-75 0.6278429472509011 1107 76-77 0.3447513116186798 1107 78-79 0.4363798480044778 1107 80-81 0.514824595714245 1107 82-83 0.4994278046652063 1107 84-85 0.358274445483584 1107 86-87 0.4402515859592917 1107 88-89 0.5409935147629419 1107 90-91 0.4248769610028269 1107 92-93 0.46799336620070164 1107 94-95 0.7863345399497135 1107 96-97 0.6733139648642457 1107 98-99 0.9273174535456441 1107 100-101 0.9602251410951759 1108 1 -0.16239531707752164 1108 2 -0.1786992490451489 1108 3 -0.13992028356013364 1108 4 -0.09481839876423948 1108 5 0.005008793315703031 1108 6 -0.06909103309276787 1108 7 -0.09770146304856553 1108 8 -0.08535053351258881 1108 9 -0.12873435722142546 1108 10-11 -0.14558563926421186 1108 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2316 68-69 1.2590313092958816 2316 70-71 1.5441939689307311 2316 72-73 0.5873063104194571 2316 74-75 0.13873659259805393 2316 76-77 -0.1797354472376469 2316 78-79 -0.26647278762439797 2316 80-81 -0.4824718738741005 2316 82-83 -0.4033195980425397 2316 84-85 -0.3645479339591766 2316 86-87 -0.5103870547651645 2316 88-89 -0.3751320464815997 2316 90-91 -0.3435028596137393 2316 92-93 -0.3120391207801312 2316 94-95 -0.1164467377889089 2316 96-97 -0.03211503210710731 2316 98-99 -0.35143198221476624 2316 100-101 -0.6544897592089534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 40.0 5 114.0 6 281.0 7 706.0 8 1470.0 9 2633.0 10 4313.0 11 6762.0 12 9085.0 13 11956.0 14 14970.0 15 18670.0 16 22730.0 17 27428.0 18 24722.0 19 19584.0 20 18787.0 21 19451.0 22 21334.0 23 23897.0 24 28554.0 25 34583.0 26 42810.0 27 52878.0 28 65420.0 29 78794.0 30 94354.0 31 113068.0 32 134730.0 33 163762.0 34 203861.0 35 258386.0 36 330099.0 37 382992.0 38 244506.0 39 21810.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.65145239838814 19.821766567825943 14.686250735302945 22.840530298482975 2 15.468690914693074 21.480451454261345 39.587200659454545 23.463656971591035 3 19.008200649874897 25.820725104198424 30.438061449264232 24.73301279666245 4 12.780999231065282 17.486383614409245 37.265288878611535 32.46732827591394 5 13.117578199700588 38.494704272188486 34.106655486041156 14.28106204206977 6 31.024035411004007 37.07541561281536 17.62584804857811 14.274700927602515 7 27.751862126244518 33.64521464160521 20.92122539868985 17.681697833460422 8 26.24363788535751 35.37339741922785 19.837315497675192 18.545649197739444 9 26.74508573902148 16.477926932798624 20.182776022359118 36.59421130582078 10-11 24.714970062754997 27.310104730348755 27.31780607962515 20.6571191272711 12-13 25.83080555713361 24.603090461448847 27.987443400083695 21.57866058133385 14-15 22.524066216401113 25.737949288715384 26.728062756594955 25.009921738288547 16-17 21.654394482217835 29.24602579259108 27.181398288895426 21.91818143629566 18-19 21.47356646355771 27.49242217084877 29.89094287137964 21.143068494213885 20-21 22.610461352829013 26.513165106526664 29.75807361449726 21.11829992614706 22-23 22.050623269196766 26.76736964316148 29.63755249919786 21.544454588443894 24-25 21.712132650493427 26.809107679308486 29.977384028635427 21.50137564156266 26-27 21.653817687183693 27.073873297193497 29.835830449480905 21.436478566141908 28-29 21.437565874133373 27.24175089320774 29.90496121615077 21.415722016508116 30-31 21.55823029993157 27.02334636966162 29.78732001535474 21.63110331505207 32-33 21.32651838533662 27.12136460591905 29.681490998919585 21.870626009824743 34-35 21.911454785688495 27.030445606696652 29.56431545645405 21.493784151160803 36-37 21.869296876595673 26.827232180223604 29.618676428832032 21.68479451434869 38-39 21.579858497063903 27.09667498534354 29.760767293824426 21.562699223768135 40-41 21.693935486764808 26.895920059608475 29.77850470602158 21.63163974760514 42-43 21.50593746032649 26.94526867453522 29.54745062262134 22.00134324251695 44-45 21.629226644454278 27.12418981180207 29.301791474527505 21.944792069216152 46-47 21.704462045046917 27.223804621299397 28.94144509408901 22.130288239564674 48-49 21.699276943046623 27.295585194000825 28.818203423813728 22.18693443913882 50-51 21.585846722346382 27.484954504118313 28.729879439817818 22.199319333717487 52-53 21.813104145180613 27.49232907775977 28.570742237795386 22.12382453926423 54-55 21.35029728277241 27.303918968552377 28.739523046545447 22.60626070212977 56-57 21.491427381114605 27.6556128977779 28.468982673470894 22.3839770476366 58-59 21.671271721296797 27.23314807082855 28.51775525143278 22.577824956441873 60-61 21.583689894988026 27.255020737524475 28.79271037804471 22.36857898944279 62-63 21.421435758537154 27.26594959422128 28.658839814924097 22.65377483231747 64-65 21.184614328989877 27.23059402155572 28.589324083755912 22.995467565698494 66-67 21.108825924749354 27.46618856686634 28.49358102545757 22.931404482926734 68-69 20.977111081751072 27.196297261578284 28.784610769521763 23.04198088714888 70-71 20.90067716917987 27.304653856732443 28.56188378708821 23.23278518699948 72-73 21.110699273518488 27.137530603985432 28.463389418935 23.28838070356108 74-75 21.155597707773083 27.285944102958126 28.011209021641697 23.547249167627097 76-77 21.087561631380463 27.22081567422494 28.060048938932784 23.631573755461808 78-79 21.12042787218567 27.292555366446376 27.931799068430585 23.655217692937374 80-81 21.00469929474572 27.423641461334235 27.881908715419417 23.68975052850063 82-83 21.093892525084414 27.287182544127763 27.688473171278144 23.930451759509673 84-85 21.02626472765098 27.04548818787382 27.671560880958573 24.256686203516633 86-87 20.97999263061161 26.941015687355495 27.92202050866383 24.15697117336906 88-89 20.928413628991414 26.90185929169764 27.71512437818907 24.454602701121882 90-91 20.8845568124285 26.977824568506552 27.760186801960597 24.377431817104352 92-93 20.99367873574715 26.7378075615123 27.768333666733348 24.5001800360072 94-95 20.640399206567338 26.551428493072475 27.941036639109246 24.867135661250934 96-97 20.746337246419174 26.332892988636065 27.997179873306365 24.923589891638393 98-99 20.666483137453245 26.296141725099915 27.74875677630937 25.288618361137477 100-101 20.44233810427384 26.286698548512454 28.022341965304737 25.24862138190897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1017.0 1 705.0 2 485.5 3 636.0 4 586.5 5 584.0 6 986.0 7 2041.5 8 3202.5 9 3692.5 10 3436.5 11 3083.5 12 3113.0 13 3282.0 14 3472.0 15 3629.0 16 3829.5 17 4056.5 18 4319.0 19 4659.0 20 5085.5 21 5952.5 22 7058.0 23 8177.5 24 9642.0 25 11630.0 26 14680.0 27 18482.5 28 21877.0 29 25890.5 30 30319.0 31 34342.0 32 39143.5 33 44366.0 34 49421.5 35 53909.5 36 59136.0 37 64413.5 38 68119.5 39 70826.0 40 73771.0 41 78502.0 42 83453.0 43 87344.0 44 90134.0 45 92618.5 46 94860.0 47 98234.0 48 105201.5 49 105807.0 50 99674.5 51 95091.0 52 89546.0 53 85342.5 54 81731.0 55 74496.0 56 65902.0 57 57331.0 58 50025.5 59 43775.0 60 37102.0 61 29831.0 62 22975.0 63 17270.5 64 12714.5 65 9465.5 66 6847.5 67 5257.5 68 3696.5 69 2239.5 70 1636.0 71 1273.5 72 1021.0 73 854.0 74 654.5 75 468.5 76 293.0 77 151.5 78 84.5 79 48.5 80 26.0 81 15.5 82 8.0 83 5.0 84 2.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023684149462985915 2 0.021083693863164825 3 1.60028036912067E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8004906459611723E-4 18-19 2.0003504614008376E-5 20-21 0.0 22-23 0.0 24-25 4.000700922801675E-5 26-27 4.80084110736201E-4 28-29 1.4002453229805862E-4 30-31 3.4005957843814236E-4 32-33 0.002260396021382946 34-35 0.00418073246432775 36-37 0.10301804876214311 38-39 0.09481661187039969 40-41 0.10407823450668557 42-43 0.10477835716817586 44-45 0.09829722167323715 46-47 0.0919761142152105 48-49 0.09271624388592882 50-51 0.09213614225212256 52-53 0.07273274277653445 54-55 0.06079065052197145 56-57 0.03286575808081576 58-59 0.012622211411439285 60-61 0.009121598103987818 62-63 0.0018803294337167871 64-65 0.005300928722712219 66-67 0.13166306736940311 68-69 0.3762659217894975 70-71 0.36194341248586753 72-73 0.15890784065368252 74-75 0.02728478029350742 76-77 0.029345141268750284 78-79 0.004960869144274077 80-81 0.0015202663506646364 82-83 8.00140184560335E-5 84-85 4.80084110736201E-4 86-87 0.0013202313045245525 88-89 3.20056073824134E-4 90-91 6.601156522622763E-4 92-93 0.0024804345721370384 94-95 0.07231266917964027 96-97 0.015342688038944422 98-99 0.016562901820398934 100-101 0.009181608617829844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2499562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.59905595121564 #Duplication Level Percentage of deduplicated Percentage of total 1 70.80164898563069 35.11694949476627 2 14.813099454129858 14.694314972726172 3 5.841803743503941 8.692438521902188 4 2.8921303767247193 5.737877454935188 5 1.6362350585814847 4.057785710996183 6 0.9992491209626612 2.973708783589805 7 0.65031822636197 2.2578619066795866 8 0.47239006314849874 1.874408093832054 9 0.315867314506207 1.410004854481823 >10 1.4248533164037696 12.176575390637007 >50 0.0939733970867893 3.222372793187278 >100 0.05383103335304218 4.950583460477623 >500 0.00371220775276103 1.2383005032562333 >1k 6.456013483048381E-4 0.47107213442796664 >5k 1.6140033707620952E-4 0.6488699440234317 >10k+ 8.070016853810476E-5 0.4768759800811279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11914 0.4766435079425916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9598 0.38398727457050474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6613 0.2645663520248748 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0007009228016746E-5 0.0 12-13 0.0 0.0 0.0 4.0007009228016746E-5 0.0 14-15 0.0 0.0 0.0 1.0001752307004187E-4 0.0 16-17 0.0 0.0 0.0 1.2002102768405025E-4 0.0 18-19 0.0 0.0 0.0 1.4002453229805862E-4 0.0 20-21 0.0 0.0 0.0 2.400420553681005E-4 0.0 22-23 0.0 0.0 0.0 8.201436891743433E-4 0.0 24-25 0.0 0.0 0.0 0.001980346956786829 0.0 26-27 0.0 0.0 0.0 0.0037806623720475826 0.0 28-29 0.0 0.0 0.0 0.011842074731492958 0.0 30-31 0.0 0.0 0.0 0.03770660619740579 0.0 32-33 0.0 0.0 0.0 0.09017579879994975 0.0 34-35 0.0 0.0 0.0 0.1731903429480845 0.0 36-37 0.0 0.0 0.0 0.3087941007264473 0.0 38-39 0.0 0.0 0.0 0.5170305837582745 0.0 40-41 0.0 0.0 0.0 0.7443504101918657 0.0 42-43 0.0 0.0 0.0 0.969729896677898 0.0 44-45 0.0 0.0 0.0 1.234936360850421 0.0 46-47 0.0 0.0 0.0 1.5447306368075686 0.0 48-49 0.0 0.0 0.0 1.8760486837293895 0.0 50-51 0.0 0.0 0.0 2.233531314686333 0.0 52-53 0.0 0.0 0.0 2.5679899118325533 0.0 54-55 0.0 0.0 0.0 2.9170710708516134 0.0 56-57 0.0 0.0 0.0 3.317521229719447 0.0 58-59 0.0 0.0 0.0 3.7308336420540877 2.0003504614008373E-5 60-61 0.0 4.0007009228016746E-5 0.0 4.165749839371857 4.0007009228016746E-5 62-63 0.0 4.0007009228016746E-5 0.0 4.574361428122208 4.0007009228016746E-5 64-65 0.0 4.0007009228016746E-5 0.0 5.000496086914428 4.0007009228016746E-5 66-67 0.0 4.0007009228016746E-5 0.0 5.436412459462898 4.0007009228016746E-5 68-69 0.0 4.0007009228016746E-5 0.0 5.923097726721721 4.0007009228016746E-5 70-71 0.0 4.0007009228016746E-5 0.0 6.492577499577926 4.0007009228016746E-5 72-73 0.0 4.0007009228016746E-5 0.0 7.05237557620095 4.0007009228016746E-5 74-75 0.0 4.0007009228016746E-5 0.0 7.616734451875969 4.0007009228016746E-5 76-77 0.0 4.0007009228016746E-5 0.0 8.196696061149915 4.0007009228016746E-5 78-79 0.0 4.0007009228016746E-5 0.0 8.839068604819564 4.0007009228016746E-5 80-81 0.0 4.0007009228016746E-5 0.0 9.538311112106841 4.0007009228016746E-5 82-83 0.0 4.0007009228016746E-5 0.0 10.230112315677706 4.0007009228016746E-5 84-85 0.0 4.0007009228016746E-5 0.0 10.893488539192067 4.0007009228016746E-5 86-87 0.0 4.0007009228016746E-5 0.0 11.556204647054162 4.0007009228016746E-5 88-89 0.0 4.0007009228016746E-5 0.0 12.2605280445134 4.0007009228016746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8445 0.0 35.12708 1 GTATCAA 13215 0.0 32.540386 1 GTACATG 13490 0.0 26.740118 1 ATCAACG 16085 0.0 26.398615 3 TCAACGC 16205 0.0 26.232409 4 TACATGG 13510 0.0 26.202911 2 TATCAAC 16235 0.0 26.101488 2 CAACGCA 16610 0.0 25.592787 5 AACGCAG 16845 0.0 25.235748 6 ACATGGG 13505 0.0 25.048063 3 ACGCAGA 19325 0.0 21.923563 7 CGCAGAG 19465 0.0 21.741507 8 CATGGGG 4610 0.0 21.612131 4 GAGTACT 9715 0.0 21.194641 12-13 CATGGGA 9315 0.0 20.67866 4 TGCACCG 515 0.0 20.267242 5 GCAGAGT 20675 0.0 20.10193 9 GTACTTT 10400 0.0 19.79865 14-15 ATTCGCG 325 0.0 19.708004 80-81 AGTACTT 10015 0.0 19.659676 12-13 >>END_MODULE