FastQCFastQC Report
Fri 27 May 2016
SRR939847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598002
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77680.4861070261489035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.4006252808194233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40790.25525625124374063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.045.9665031
GTATCAA78550.034.9065251
TATCAAC96850.028.800672
ATCAACG97900.028.494463
TCAACGC99550.028.1651444
CAACGCA100800.027.7688085
AACGCAG103450.027.1491956
TACCTGG11650.026.4674642
TACATGG82300.025.7651442
GTACATG81450.025.6845761
GGGCGTA2850.024.9704159
ACGCAGA117050.023.6704867
ACATGGG83900.023.4666943
CGCAGAG117250.023.3468748
GAGTACT65200.022.5565712-13
GCAGAGT121500.021.3985169
GTACTTT68800.021.17140814-15
AGTACAT62400.020.297922
CAGAGTA117600.019.9283710-11
AGTACTT67900.019.87794312-13
Produced by FastQC (version 0.11.5)