FastQCFastQC Report
Fri 27 May 2016
SRR939847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598002
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.5282846955135225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.4755938978799776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.31583189507897985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.11727144271408921No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16690.10444292310022141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.10406745423347406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.052.86591
GTATCAA76750.037.804771
TATCAAC98750.029.3273162
ATCAACG98350.029.2538433
TCAACGC100100.028.740614
CAACGCA101050.028.4704115
AACGCAG104200.027.7203526
GTACATG85050.027.458751
TACATGG84650.027.3587072
ACATGGG85250.026.0034483
ACGCAGA117950.024.3682487
CGCAGAG118450.024.2256038
GTGGTAT22300.024.1184751
TGGTATC21700.023.4749722
TACCTGG12250.023.1023522
GAGTACT73100.022.36753312-13
GCAGAGT126600.022.2566539
CATGGGA48300.022.1655734
CATGGGG38150.022.0052194
GTACTTT77000.021.20469714-15
Produced by FastQC (version 0.11.5)