##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939847_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1598002 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.408399363705428 31.0 31.0 34.0 30.0 34.0 2 31.609423517617625 31.0 31.0 34.0 30.0 34.0 3 31.658444107078715 31.0 31.0 34.0 30.0 34.0 4 35.476367363745474 37.0 35.0 37.0 33.0 37.0 5 35.2101668208175 37.0 35.0 37.0 32.0 37.0 6 35.24422247281292 37.0 35.0 37.0 32.0 37.0 7 35.15808303118519 37.0 35.0 37.0 32.0 37.0 8 35.10105306501494 37.0 35.0 37.0 32.0 37.0 9 36.692425916863684 39.0 35.0 39.0 32.0 39.0 10-11 36.552605065575634 38.5 35.0 39.0 32.0 39.0 12-13 36.486069792152946 38.0 35.0 39.0 32.0 39.0 14-15 37.504073211422764 39.0 36.0 41.0 32.0 41.0 16-17 37.4303173588018 39.0 36.0 41.0 32.0 41.0 18-19 37.42709896483233 39.0 36.0 41.0 32.0 41.0 20-21 37.368099977346716 39.0 36.0 41.0 31.5 41.0 22-23 37.21701850185419 39.0 36.0 40.0 31.0 41.0 24-25 37.04551183290133 39.0 36.0 40.0 31.0 41.0 26-27 36.747803194238806 39.0 35.5 40.0 30.0 41.0 28-29 36.50731131750774 38.0 35.0 40.0 30.0 41.0 30-31 36.23149626846525 38.0 35.0 40.0 30.0 41.0 32-33 36.14955456876775 38.0 35.0 40.0 30.0 41.0 34-35 36.19747628601216 38.0 35.0 40.0 30.0 41.0 36-37 36.14531740260651 38.0 35.0 40.0 30.0 41.0 38-39 36.00226470304793 38.0 35.0 40.0 29.5 41.0 40-41 35.81048646997939 38.0 35.0 40.0 29.0 41.0 42-43 35.572192650572404 38.0 34.0 40.0 27.5 41.0 44-45 35.27796585986751 38.0 34.0 40.0 27.0 41.0 46-47 34.91093878480753 38.0 33.5 40.0 26.0 41.0 48-49 34.54902872461987 38.0 33.0 40.0 24.5 41.0 50-51 34.25382415040782 38.0 33.0 40.0 24.0 41.0 52-53 33.9473883637192 37.5 33.0 40.0 23.5 41.0 54-55 33.61608026773433 37.0 33.0 40.0 22.5 41.0 56-57 32.20797658576147 36.5 30.5 39.5 14.5 41.0 58-59 28.740129549274656 34.0 23.0 38.0 7.0 40.0 60-61 28.730261914565816 33.5 23.0 38.0 6.5 40.0 62-63 30.657584596264584 34.5 28.5 38.0 4.5 40.0 64-65 30.216360492665217 35.0 29.0 38.0 2.0 40.0 66-67 24.762102300247435 28.0 16.5 35.5 2.0 39.0 68-69 17.161869008924896 13.0 7.0 29.0 2.0 35.5 70-71 16.053461760373267 12.5 7.5 25.5 2.0 33.0 72-73 18.099489550075656 17.0 7.0 30.0 2.0 34.0 74-75 18.786053459257246 17.5 7.5 30.5 2.0 35.0 76-77 19.74223061047483 22.5 7.5 30.5 2.0 34.5 78-79 22.773803474588895 27.5 10.5 33.5 2.0 35.5 80-81 25.40663685026677 31.5 20.0 34.5 2.0 36.0 82-83 26.43559488661466 32.5 25.0 35.0 2.0 36.0 84-85 26.445137427863045 33.0 25.0 35.0 2.0 36.0 86-87 26.156170017309115 33.0 24.0 35.0 2.0 35.0 88-89 25.71578133193826 32.5 22.0 35.0 2.0 35.0 90-91 24.054731470924317 31.5 10.0 34.0 2.0 35.0 92-93 21.892592437306085 30.5 2.0 34.0 2.0 35.0 94-95 17.51272213676829 20.0 2.0 33.0 2.0 35.0 96-97 16.201086419165932 12.0 2.0 32.5 2.0 34.5 98-99 15.134021734641134 2.0 2.0 31.5 2.0 34.0 100-101 14.528723681196894 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36998991459728003 1101 2 0.38695914337393233 1101 3 0.37764346075372046 1101 4 0.11182556388071418 1101 5 0.29769741722751775 1101 6 0.5300923613930735 1101 7 0.49111741853401725 1101 8 0.41704621271585296 1101 9 0.5281450440505893 1101 10-11 0.503657883741667 1101 12-13 0.6465831119704291 1101 14-15 0.7435437532457385 1101 16-17 0.6344174552724482 1101 18-19 0.807932136044947 1101 20-21 0.7578568510146084 1101 22-23 0.6336560966054989 1101 24-25 0.47718696577162945 1101 26-27 0.5810557798408382 1101 28-29 0.7681701661783933 1101 30-31 0.3914741112031379 1101 32-33 0.6029691304968523 1101 34-35 0.6496400910341222 1101 36-37 0.5585550571510751 1101 38-39 0.5312825783622799 1101 40-41 0.5711754397301334 1101 42-43 0.6471724089098245 1101 44-45 0.3560247255333522 1101 46-47 0.003695446893274834 1101 48-49 -0.13108908576921863 1101 50-51 0.0166368615207233 1101 52-53 0.3953109463311151 1101 54-55 0.3782603876437918 1101 56-57 0.6746060070439697 1101 58-59 -8.470320032937718 1101 60-61 -9.608409091405953 1101 62-63 -2.812730869974615 1101 64-65 -0.07945272561000039 1101 66-67 -1.122641099057418 1101 68-69 -3.7057484465249217 1101 70-71 -4.294970189515382 1101 72-73 -6.433137519051499 1101 74-75 -7.249508374486281 1101 76-77 -8.362955027085878 1101 78-79 -11.828110343889291 1101 80-81 -7.767832793894783 1101 82-83 -3.8350246973765714 1101 84-85 -3.011472192637804 1101 86-87 -2.6690733068105494 1101 88-89 -2.559146482622456 1101 90-91 -7.995478396384991 1101 92-93 -14.136383933152134 1101 94-95 -11.925017980696712 1101 96-97 -11.547069441293376 1101 98-99 -11.205605470810003 1101 100-101 -11.072452280945026 1102 1 0.40499444414929187 1102 2 0.5036320642337522 1102 3 0.40666007416498573 1102 4 0.37053121122760757 1102 5 0.40272616756391955 1102 6 0.48273769557546586 1102 7 0.4996278592461607 1102 8 0.4990235232611795 1102 9 0.5954970186699384 1102 10-11 0.6428236312314581 1102 12-13 0.6407747669815578 1102 14-15 0.7888525398185564 1102 16-17 0.7833160539541382 1102 18-19 0.7561943231931494 1102 20-21 0.8260938887254952 1102 22-23 0.8151504128705724 1102 24-25 0.8001501835350524 1102 26-27 0.9921078867458419 1102 28-29 0.8487535497347949 1102 30-31 0.7501141842824381 1102 32-33 0.7674202966195907 1102 34-35 0.772940087622743 1102 36-37 0.7232592338149786 1102 38-39 0.6666759102552504 1102 40-41 0.7052691313339778 1102 42-43 0.7086563394238539 1102 44-45 0.548311315406103 1102 46-47 0.4319276328664472 1102 48-49 0.722099889414892 1102 50-51 0.6436993238534185 1102 52-53 0.6133937139442551 1102 54-55 0.6011337724510355 1102 56-57 1.4012693381409562 1102 58-59 -8.108766074412493 1102 60-61 -9.119385200388113 1102 62-63 -2.4353173199808715 1102 64-65 0.20236548205195604 1102 66-67 -1.2712393069467964 1102 68-69 -3.9220255527666055 1102 70-71 -4.064567608997383 1102 72-73 -6.872965073889404 1102 74-75 -7.704101510793578 1102 76-77 -8.683185351962782 1102 78-79 -11.6037885448043 1102 80-81 -7.789902988684471 1102 82-83 -3.9401131468294253 1102 84-85 -3.036886011751374 1102 86-87 -2.8160387912451874 1102 88-89 -3.050087378601958 1102 90-91 -8.422029057244734 1102 92-93 -14.06668331948312 1102 94-95 -12.133714720556389 1102 96-97 -12.140988944141876 1102 98-99 -11.599480815264428 1102 100-101 -11.289178078165131 1103 1 0.13191283172627166 1103 2 0.06836868637952875 1103 3 0.12670949960716982 1103 4 0.07189757774956007 1103 5 0.10729948887765062 1103 6 -0.018247532054431304 1103 7 0.12399018124030903 1103 8 0.2130122013464728 1103 9 0.18038029380489462 1103 10-11 0.21593986909527985 1103 12-13 0.08230914892175889 1103 14-15 0.14523380995915858 1103 16-17 0.18750849541901005 1103 18-19 0.26487356554360986 1103 20-21 0.2686864621297076 1103 22-23 0.21424747462992855 1103 24-25 0.22418360701541928 1103 26-27 0.02100070157831624 1103 28-29 0.3242549710184761 1103 30-31 0.2249774958216122 1103 32-33 0.1745792144407261 1103 34-35 0.1995855300167193 1103 36-37 -0.08833087695189334 1103 38-39 -0.06161756496467774 1103 40-41 -0.26521116929428956 1103 42-43 -0.04921555162603397 1103 44-45 0.04173032295980761 1103 46-47 -0.2872816291367428 1103 48-49 -0.10947324538773273 1103 50-51 0.030753899074390745 1103 52-53 -0.13531538745215954 1103 54-55 -0.19530187949161615 1103 56-57 0.49034911308422124 1103 58-59 -7.040937339959406 1103 60-61 -8.99325447682865 1103 62-63 -2.632512659275651 1103 64-65 -0.20186754284727826 1103 66-67 -1.916234851403793 1103 68-69 -4.0767041319664745 1103 70-71 -4.636784535225335 1103 72-73 -7.5113912828440075 1103 74-75 -7.9875737776287945 1103 76-77 -9.04673305217727 1103 78-79 -11.953606523783805 1103 80-81 -8.45216894315504 1103 82-83 -4.605301540312588 1103 84-85 -3.760832310369466 1103 86-87 -3.5480892563140785 1103 88-89 -3.6128190063948757 1103 90-91 -9.096224814462541 1103 92-93 -14.607723965087848 1103 94-95 -12.215292604484315 1103 96-97 -12.079278378287915 1103 98-99 -11.638313549344495 1103 100-101 -11.445118629478134 1104 1 0.3028744594647712 1104 2 0.271063497238611 1104 3 0.3193366555889199 1104 4 0.2036479111449836 1104 5 0.06045040833936355 1104 6 0.07287734855896133 1104 7 0.11522949558414552 1104 8 0.28344808445256575 1104 9 0.1448843995215583 1104 10-11 0.26230955509740284 1104 12-13 0.2992320347807649 1104 14-15 0.3580140173592028 1104 16-17 0.48712527548448037 1104 18-19 0.38110363943763303 1104 20-21 0.4042708429604289 1104 22-23 0.4523247070486818 1104 24-25 0.4403598645297535 1104 26-27 0.38439321814459504 1104 28-29 0.23431065027285314 1104 30-31 0.44265846818305477 1104 32-33 0.31395365816565146 1104 34-35 0.4109859534510889 1104 36-37 0.3711745150353849 1104 38-39 0.3866192832879136 1104 40-41 0.37840205356761913 1104 42-43 0.2970906340049879 1104 44-45 0.3501950185276428 1104 46-47 0.2856144313268416 1104 48-49 0.1407666366893494 1104 50-51 0.022996726267628276 1104 52-53 0.10951698339591331 1104 54-55 -0.07923369827825866 1104 56-57 0.8584993524488311 1104 58-59 -7.017943774269412 1104 60-61 -8.787689212640498 1104 62-63 -3.102117493287601 1104 64-65 -0.8316459642531875 1104 66-67 -2.1136762817367156 1104 68-69 -4.029485803053772 1104 70-71 -4.533793068965544 1104 72-73 -7.642741689571647 1104 74-75 -8.56179602744916 1104 76-77 -9.12680524596355 1104 78-79 -11.886719343022866 1104 80-81 -8.457783810077778 1104 82-83 -4.585053497070241 1104 84-85 -3.5737590343417054 1104 86-87 -3.3934776804699425 1104 88-89 -3.6743493135917795 1104 90-91 -9.123155551007708 1104 92-93 -14.66475528208111 1104 94-95 -12.376893286946974 1104 96-97 -12.258013811642833 1104 98-99 -11.612005249498553 1104 100-101 -11.168830658621774 1105 1 0.17368772317448844 1105 2 0.15079031553917233 1105 3 0.16246631342935558 1105 4 0.18292299780760146 1105 5 0.14282538962728353 1105 6 0.11585863060798118 1105 7 0.07958921955528098 1105 8 0.15300800567103323 1105 9 0.29926053141898024 1105 10-11 0.31565696285051814 1105 12-13 0.17107409601550216 1105 14-15 0.2544085296563452 1105 16-17 0.19722231413262392 1105 18-19 0.34932172103111014 1105 20-21 0.38134487528903094 1105 22-23 0.38227144733087215 1105 24-25 0.27964877611773886 1105 26-27 0.3662546078577904 1105 28-29 0.3952154637537717 1105 30-31 0.4390441014145381 1105 32-33 0.4143711210129979 1105 34-35 0.5190122619024891 1105 36-37 0.4227310666117461 1105 38-39 0.3544979791072649 1105 40-41 0.35710220238369317 1105 42-43 0.3645818063058215 1105 44-45 0.19646170163159127 1105 46-47 0.19455924609926 1105 48-49 0.3571703802795412 1105 50-51 0.2610866704689343 1105 52-53 0.17722206046033762 1105 54-55 0.1482963951500551 1105 56-57 0.899399924524829 1105 58-59 -6.724452863203897 1105 60-61 -8.418529950087258 1105 62-63 -2.4987732409049315 1105 64-65 -0.2539090106092452 1105 66-67 -1.7172564530953451 1105 68-69 -3.9669159079294793 1105 70-71 -4.477752589231827 1105 72-73 -5.987684584704745 1105 74-75 -6.4330481077922315 1105 76-77 -8.401314035504743 1105 78-79 -11.584190130163044 1105 80-81 -7.678426023357339 1105 82-83 -3.7632457046261045 1105 84-85 -2.841822429402889 1105 86-87 -2.5904538816387515 1105 88-89 -2.8407019108285567 1105 90-91 -8.17320376213281 1105 92-93 -11.005221954031187 1105 94-95 -11.19433309275227 1105 96-97 -11.607384498691726 1105 98-99 -11.122633344329909 1105 100-101 -10.914024222253122 1106 1 0.09106838412920126 1106 2 0.07123095201407992 1106 3 -0.012564286325847718 1106 4 -0.011079450172815086 1106 5 -0.09340472053183646 1106 6 -0.1185354942390191 1106 7 0.043481508416967074 1106 8 0.07222465193786576 1106 9 -0.007961010808685387 1106 10-11 0.12869245856655454 1106 12-13 0.0743788695773091 1106 14-15 0.21309886013370516 1106 16-17 0.04728351364303052 1106 18-19 0.11156162311189632 1106 20-21 0.022715744322084674 1106 22-23 0.05057010094164127 1106 24-25 0.048926621894977984 1106 26-27 0.2254957339287813 1106 28-29 -0.002275356319671573 1106 30-31 0.14039049064342635 1106 32-33 0.2356685506335623 1106 34-35 0.0909216866270981 1106 36-37 0.22138467738286494 1106 38-39 0.3701038542602646 1106 40-41 0.3662821289442775 1106 42-43 0.3125622224624962 1106 44-45 0.3721042964908321 1106 46-47 0.4102875202730587 1106 48-49 0.44560367281320623 1106 50-51 0.4863008687553574 1106 52-53 0.6071486210478483 1106 54-55 0.35698672562740796 1106 56-57 1.1114562280744025 1106 58-59 -6.001074650229601 1106 60-61 -8.70494365877759 1106 62-63 -2.22153945877519 1106 64-65 0.18121950836260226 1106 66-67 -1.1667668570121137 1106 68-69 -3.996291672923359 1106 70-71 -4.612697509672463 1106 72-73 -6.066019958021759 1106 74-75 -6.463953860546209 1106 76-77 -8.38938013097598 1106 78-79 -11.332048147298906 1106 80-81 -7.70565980548708 1106 82-83 -3.891764676474331 1106 84-85 -2.8990439716305545 1106 86-87 -2.6057537592397324 1106 88-89 -2.781644383288775 1106 90-91 -8.148723957971242 1106 92-93 -11.962076299196426 1106 94-95 -11.643231501565 1106 96-97 -11.988657448508128 1106 98-99 -11.416696991820729 1106 100-101 -11.182384075374296 1107 1 0.004857127608982381 1107 2 0.1596215994121266 1107 3 0.030640473170549853 1107 4 0.01280107760936744 1107 5 0.09898095309671362 1107 6 0.01638024795093429 1107 7 0.12268025869004617 1107 8 0.06709451693982516 1107 9 0.09141861469220913 1107 10-11 0.0948176317656575 1107 12-13 0.021418100109428906 1107 14-15 -0.0744849712249831 1107 16-17 -0.007417476621903063 1107 18-19 0.0011630930971975317 1107 20-21 0.07813908333145747 1107 22-23 0.00834468324376303 1107 24-25 0.09219609725506928 1107 26-27 0.15980613964113388 1107 28-29 0.07870139684429489 1107 30-31 0.0861219370167845 1107 32-33 -0.07931852115690674 1107 34-35 -0.15701563549779962 1107 36-37 -0.07615417458016083 1107 38-39 0.04657615886325317 1107 40-41 -0.1149951602017083 1107 42-43 -0.14543456344097194 1107 44-45 -0.10610815409318519 1107 46-47 -0.028082777165536754 1107 48-49 -0.1375427451450406 1107 50-51 -0.13565337076965278 1107 52-53 -0.08057788447506908 1107 54-55 -0.3978714531110725 1107 56-57 0.14155359365244635 1107 58-59 -7.005897665435214 1107 60-61 -9.098317726943968 1107 62-63 -2.8290579458155563 1107 64-65 -0.7698433281224801 1107 66-67 -2.127123714499554 1107 68-69 -4.2685075440961455 1107 70-71 -4.9586052974223875 1107 72-73 -6.287200450191513 1107 74-75 -6.826340626488754 1107 76-77 -9.074157388699238 1107 78-79 -12.3646309013068 1107 80-81 -8.639610720187832 1107 82-83 -4.630165573668133 1107 84-85 -3.6481963060402407 1107 86-87 -3.438988460110288 1107 88-89 -3.556383050905108 1107 90-91 -8.435159737389652 1107 92-93 -11.887938105015916 1107 94-95 -11.896961828690596 1107 96-97 -12.30513186292308 1107 98-99 -11.785180889915315 1107 100-101 -11.290660822852248 1108 1 -0.18605183930830904 1108 2 -0.08734513698143687 1108 3 -0.1737134140441583 1108 4 -0.18085939786394079 1108 5 -0.2829923946701385 1108 6 -0.030212346449566496 1108 7 -0.11145926497522396 1108 8 -0.17942248814397033 1108 9 -0.09064755072564168 1108 10-11 -0.03863753221219213 1108 12-13 -0.15460630382609963 1108 14-15 -0.18667014136196514 1108 16-17 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5.862624480887078 2312 80-81 3.899525188084514 2312 82-83 2.529577478561624 2312 84-85 2.0048275809021803 2312 86-87 1.6914224406702587 2312 88-89 1.8773145099885333 2312 90-91 3.291653838594012 2312 92-93 5.3164144213314195 2312 94-95 9.364925629054245 2312 96-97 9.920494927757398 2312 98-99 10.564963484792365 2312 100-101 9.577419763720764 2313 1 -0.33036056742055564 2313 2 -0.35983268925657796 2313 3 -0.32832680596256125 2313 4 -0.3254807904109924 2313 5 -0.32638444901473207 2313 6 -0.3150529621896183 2313 7 -0.39441394719973744 2313 8 -0.3800134670290092 2313 9 -0.4201139688080602 2313 10-11 -0.48774816270351096 2313 12-13 -0.48172027846744925 2313 14-15 -0.5358393922902636 2313 16-17 -0.4729712435735678 2313 18-19 -0.590680988196219 2313 20-21 -0.6241945270360674 2313 22-23 -0.6731925527741751 2313 24-25 -0.47872045562969845 2313 26-27 -0.5519294910511263 2313 28-29 -0.5488643907220165 2313 30-31 -0.6502135357383736 2313 32-33 -0.4922203185197418 2313 34-35 -0.5366017132873395 2313 36-37 -0.6487389725822013 2313 38-39 -0.902174357124423 2313 40-41 -1.3735940344857127 2313 42-43 -1.114099442730918 2313 44-45 -0.8195142733274068 2313 46-47 -0.769359996809925 2313 48-49 -0.7446201978294908 2313 50-51 -0.3516007724696877 2313 52-53 -0.6566902089529734 2313 54-55 -0.7330396202261937 2313 56-57 0.2891193398016938 2313 58-59 3.7630439144051664 2313 60-61 3.694769315173051 2313 62-63 1.0534934217071097 2313 64-65 1.1625533121246967 2313 66-67 2.088116490708231 2313 68-69 0.8007756230396836 2313 70-71 3.145771071929534 2313 72-73 8.394701083482637 2313 74-75 9.836590853108504 2313 76-77 8.852292277824308 2313 78-79 6.368872851770142 2313 80-81 3.602136238073424 2313 82-83 2.0603048069133365 2313 84-85 1.5275480622734925 2313 86-87 1.5065952030639949 2313 88-89 1.5007590469247738 2313 90-91 3.090992554562046 2313 92-93 4.779239832386324 2313 94-95 8.45174713216889 2313 96-97 9.282504761640832 2313 98-99 9.761300453764925 2313 100-101 8.937383297373225 2314 1 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4.113681895132672 2314 60-61 3.857808229451205 2314 62-63 1.6418662193452391 2314 64-65 1.4300552249939429 2314 66-67 2.148306134599075 2314 68-69 0.05815350127134522 2314 70-71 2.6614762969370815 2314 72-73 8.443533136285193 2314 74-75 9.829139550804065 2314 76-77 8.871926851738891 2314 78-79 6.406779210374914 2314 80-81 3.446570244965198 2314 82-83 1.9770444706134676 2314 84-85 1.6091507920796317 2314 86-87 1.4816869070885978 2314 88-89 1.7209230108062172 2314 90-91 2.9729287273438594 2314 92-93 4.95069200841321 2314 94-95 8.699717120699685 2314 96-97 9.410512626423957 2314 98-99 9.621642262440844 2314 100-101 8.764111133151841 2315 1 -0.7555015534129126 2315 2 -0.5648488846311501 2315 3 -0.5802162663293728 2315 4 -0.45469176499216957 2315 5 -0.7102679840716348 2315 6 -0.828289225653279 2315 7 -0.7666807562502456 2315 8 -0.9927254775943908 2315 9 -0.9120800707432366 2315 10-11 -0.9335757569757064 2315 12-13 -0.8463052114533269 2315 14-15 -0.7641870860736475 2315 16-17 -0.8480069773504297 2315 18-19 -0.8323618105574226 2315 20-21 -0.9444896585009275 2315 22-23 -0.9083810499237472 2315 24-25 -0.9642934600311577 2315 26-27 -1.0550657944421147 2315 28-29 -0.9077921647853202 2315 30-31 -1.0677836861628052 2315 32-33 -1.101729873908866 2315 34-35 -0.9644856358046994 2315 36-37 -1.0894846922073143 2315 38-39 -0.9872963481041594 2315 40-41 -0.9132803805209804 2315 42-43 -0.8447978874551865 2315 44-45 -0.7196073583395375 2315 46-47 -0.6827425029703136 2315 48-49 -0.8120394985578656 2315 50-51 -0.8583399855942062 2315 52-53 -0.7011902786486033 2315 54-55 -0.6453955924329904 2315 56-57 0.6496607449128646 2315 58-59 4.044882414271683 2315 60-61 3.549288545260776 2315 62-63 1.0275312085518813 2315 64-65 1.092422969482076 2315 66-67 1.9431826543063195 2315 68-69 5.88746163122833 2315 70-71 4.949227030197207 2315 72-73 8.971427704142311 2315 74-75 9.98681565105024 2315 76-77 8.765124306628689 2315 78-79 5.98970617525946 2315 80-81 3.179485394283777 2315 82-83 1.5289075158289442 2315 84-85 1.0307481548859911 2315 86-87 1.1386554295393587 2315 88-89 1.2537931694331554 2315 90-91 2.711931012138667 2315 92-93 4.59300838146504 2315 94-95 8.761876151775805 2315 96-97 9.23285075910344 2315 98-99 9.297813876670922 2315 100-101 8.53459452711129 2316 1 -0.4024073044289267 2316 2 -0.43864787804619 2316 3 -0.5498588970598703 2316 4 -0.26349815809587795 2316 5 -0.49643349630162703 2316 6 -0.3311363388868074 2316 7 -0.401887292268988 2316 8 -0.5062816291027943 2316 9 -0.6298658537517596 2316 10-11 -0.544968152148563 2316 12-13 -0.512979158831186 2316 14-15 -0.5744442940665166 2316 16-17 -0.8705864161248016 2316 18-19 -0.847882155883326 2316 20-21 -0.5998461842734031 2316 22-23 -0.8028043447900686 2316 24-25 -2.261039443831045 2316 26-27 -1.4537795591416014 2316 28-29 -1.1538141440093739 2316 30-31 -0.7459387823587633 2316 32-33 -0.6238453005283588 2316 34-35 -0.7506253991780341 2316 36-37 -0.8032453073954429 2316 38-39 -1.1434951278569656 2316 40-41 -0.7543692694808968 2316 42-43 -0.7597355627915832 2316 44-45 -0.6485204476021238 2316 46-47 -0.8021356894321627 2316 48-49 -0.8818917072237582 2316 50-51 -0.8052438897956691 2316 52-53 -0.8687597535033333 2316 54-55 -0.7346505412744548 2316 56-57 0.509324681490412 2316 58-59 3.8328135877554033 2316 60-61 3.7573442792825453 2316 62-63 1.3840422420257497 2316 64-65 0.9638988832912396 2316 66-67 6.022910171026208 2316 68-69 8.806318815283642 2316 70-71 5.699289448977321 2316 72-73 9.222092646168317 2316 74-75 10.008009091022906 2316 76-77 8.50753487552215 2316 78-79 6.195146180601018 2316 80-81 3.1607272634163657 2316 82-83 1.7426175211953598 2316 84-85 1.394762405191159 2316 86-87 1.390108819314186 2316 88-89 1.385571733527744 2316 90-91 2.8672184599439596 2316 92-93 4.597405043134653 2316 94-95 8.560708503278601 2316 96-97 9.226923372299849 2316 98-99 9.623813047735318 2316 100-101 8.498127753258347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 47.0 5 93.0 6 203.0 7 469.0 8 1165.0 9 1923.0 10 3152.0 11 4674.0 12 6756.0 13 9215.0 14 11816.0 15 16382.0 16 21784.0 17 28141.0 18 29199.0 19 29224.0 20 29755.0 21 30002.0 22 28378.0 23 27463.0 24 28214.0 25 30173.0 26 39954.0 27 57526.0 28 80460.0 29 104316.0 30 122750.0 31 128753.0 32 131814.0 33 135463.0 34 136103.0 35 130680.0 36 107391.0 37 64099.0 38 19607.0 39 834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35041444702946 19.287325112617054 14.219010046498934 23.143250393854554 2 16.27867972208628 20.95943065486956 38.568476493611016 24.193413129433143 3 19.552172022015917 24.964782672636872 29.961293794803918 25.521751510543293 4 13.359745482170862 16.893908768574757 35.64957991291625 34.09676583633813 5 13.49625253687192 37.92511586170361 33.64964522930317 14.928986372121303 6 32.56314142678348 35.759324155193994 16.81276595744681 14.86476846057572 7 28.654970394279854 33.093825915111495 20.10617007988726 18.145033610721388 8 27.1040675844806 34.84762202753442 19.195181476846056 18.853128911138924 9 27.049465520068182 15.940530737758776 19.589149450376155 37.42085429179688 10-11 25.580381000774715 26.554409819261803 26.45394060833466 21.41126857162882 12-13 26.248903318018375 23.873124063674513 27.502844176665608 22.3751284416415 14-15 22.960036620764512 25.092420183848503 25.94573656266065 26.00180663272634 16-17 22.297969589524918 28.55037102581849 26.532351023340397 22.619308361316193 18-19 22.17768329726692 26.564915161535392 29.38986018580982 21.86754135538787 20-21 22.934764787528426 25.612671323314988 29.541264654236976 21.911299234919607 22-23 22.637549890425667 25.73867241718095 29.40021351662889 22.223564175764484 24-25 22.44002197744433 26.0290662965378 29.4674537328489 22.06345799316897 26-27 22.156122174082142 26.32674561334069 29.41385739723786 22.10327481533931 28-29 21.915500502511303 26.422832912175043 29.61959956795292 22.042067017360733 30-31 22.06633355529632 26.173504128010894 29.51330651419645 22.24685580249633 32-33 21.920389787599927 26.27167672560394 29.339702133637797 22.46823135315834 34-35 22.42491865529275 26.397738149361366 29.142009288581516 22.035333906764368 36-37 22.511407136255617 26.034717003807962 29.18367813206833 22.27019772786809 38-39 22.129127602813725 26.27284836360586 29.29247532858704 22.30554870499337 40-41 22.209004160403214 26.028575425734786 29.50841016136155 22.25401025250045 42-43 22.110853633192868 26.146976491473183 29.172488087994854 22.569681787339096 44-45 22.045812268413684 26.440033013226888 29.045380041770436 22.468774676588996 46-47 22.216685863087786 26.60536331313536 28.535949471681104 22.642001352095747 48-49 22.193211896848574 26.718613997798457 28.407017021118104 22.68115708423486 50-51 22.20664669284983 26.8234401597655 28.260353162870484 22.70955998451419 52-53 22.412174350641628 26.79747930402953 28.132029838751073 22.65831650657777 54-55 21.982194107325427 26.770833235538305 28.259727319299046 22.987245337837226 56-57 22.08038632567555 26.986828951862847 28.124371296328217 22.808413426133388 58-59 20.709865548602682 26.396828649338776 29.86173415506833 23.031571646990212 60-61 22.08528265076622 26.59394166893167 28.516162726393357 22.80461295390875 62-63 21.86861894889402 26.63950968496107 28.496557869777906 22.995313496367007 64-65 21.154984360474536 26.540038156494266 28.92022886376803 23.38474861926317 66-67 21.294537608478368 26.5929780584157 29.052085481747074 23.060398851358855 68-69 22.04407954377415 25.87622237795508 29.272681098388166 22.8070169798826 70-71 21.210489407402267 26.276152928901 29.361534213494302 23.151823450202432 72-73 21.037861816003236 26.358050854209225 29.288971602841308 23.31511572694623 74-75 21.516386865848457 26.55543907559754 28.628054990544786 23.30011906800922 76-77 20.00797293000432 26.933063772366523 29.21547317915838 23.84349011847078 78-79 20.986469646725247 27.111073825292483 28.103494540036483 23.798961987945788 80-81 21.537267736651653 27.304955107877205 27.446726293376557 23.71105086209459 82-83 21.882377383486425 27.21457221070957 27.043765005079784 23.859285400724218 84-85 21.81163272477074 27.00684386519278 26.962507208226782 24.219016201809698 86-87 21.859493814265893 26.924355002124596 27.149205451230983 24.066945732378528 88-89 21.860485781619797 26.93745064148856 26.971055105062447 24.231008471829195 90-91 21.477496089521424 27.114543468089618 27.162209285552112 24.245751156836846 92-93 21.206291355545087 26.886546289531367 27.34149450567361 24.56566784924994 94-95 19.996361722147764 26.785045917411725 28.43951157070603 24.77908078973448 96-97 19.872643633148016 26.667655202502516 28.615102482967558 24.84459868138191 98-99 20.144233807266982 26.6895102685624 28.003222748815165 25.163033175355448 100-101 20.04007147273029 26.782954356971604 28.042711490422555 25.13426267987555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 804.0 1 601.0 2 410.0 3 468.5 4 533.0 5 573.5 6 760.5 7 1202.0 8 1664.0 9 1975.5 10 2138.5 11 2233.5 12 2439.0 13 2654.5 14 2861.0 15 3052.5 16 3121.5 17 3192.5 18 3377.0 19 3624.5 20 3931.5 21 4348.0 22 4901.5 23 5644.0 24 6557.5 25 7789.5 26 9440.5 27 11277.0 28 13116.0 29 15077.5 30 17203.0 31 19632.0 32 22533.0 33 25306.0 34 27924.5 35 30738.0 36 33618.5 37 36282.0 38 38534.0 39 40743.0 40 42961.0 41 45766.5 42 48618.0 43 51137.0 44 53851.0 45 56489.5 46 58674.5 47 60533.5 48 62837.5 49 64311.5 50 64092.0 51 63340.5 52 62263.5 53 60849.5 54 58332.0 55 54079.0 56 49019.5 57 43724.0 58 38209.5 59 32580.0 60 26954.5 61 21834.0 62 17274.0 63 12975.0 64 9605.0 65 7260.0 66 5366.5 67 3861.5 68 2729.0 69 1886.5 70 1353.5 71 983.5 72 742.0 73 581.0 74 431.5 75 277.5 76 151.0 77 75.0 78 45.0 79 28.0 80 15.0 81 8.5 82 7.5 83 4.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02071336581556218 2 0.006007501867957612 3 0.00419273567867875 4 0.0 5 0.06601994240307583 6 1.251562889157836E-4 7 0.0 8 1.251562889157836E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 9.386721668683769E-5 16-17 0.0 18-19 0.001501875466989403 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1902350560262126E-4 28-29 0.00175218804482097 30-31 0.008635783935189068 32-33 2.1902350560262126E-4 34-35 0.0011576956724709982 36-37 0.02675215675574874 38-39 0.025281570360988284 40-41 0.027565672633701337 42-43 0.028410477583882876 44-45 0.028817235522859173 46-47 0.030162665628703844 48-49 0.030444267278764356 50-51 0.02568832829996458 52-53 0.021777194271346344 54-55 0.01705254436477551 56-57 0.15375450093304013 58-59 0.7463695289492753 60-61 0.3080722051662013 62-63 5.632033001210262E-4 64-65 0.13801609760188033 66-67 1.3909244168655608 68-69 2.667831454528843 70-71 2.151843364401296 72-73 1.815204236290067 74-75 1.5351357507687724 76-77 1.1047858513318507 78-79 0.418710364567754 80-81 2.816016500605131E-4 82-83 9.386721668683769E-5 84-85 9.386721668683769E-5 86-87 0.0031914853673524813 88-89 0.0 90-91 0.22327881942575792 92-93 0.17305985849829975 94-95 1.272370122190085 96-97 1.2459621452288545 98-99 0.9700863953862385 100-101 0.3971209047297813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1598002.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.20241484139835 #Duplication Level Percentage of deduplicated Percentage of total 1 77.88854668790263 43.77524412347128 2 12.594730478089891 14.15708534290423 3 4.044545741361586 6.819397128030448 4 1.7560031088972596 3.9476646075611597 5 0.9639226316097784 2.7087389808372584 6 0.5781264257629019 1.9495260726900898 7 0.3900418033570644 1.5344903866432598 8 0.27265732357199113 1.2259200007150743 9 0.20259451562332284 1.0247670910488735 >10 1.1658871259594445 12.16278786735483 >50 0.09508061739139792 3.722100591302832 >100 0.04519221900560952 4.567682641925782 >500 0.002003491100375273 0.759681669398619 >1k 3.3391518339587884E-4 0.32566722421683225 >5k 3.3391518339587884E-4 1.319246271899496 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8442 0.5282846955135225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7600 0.4755938978799776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5047 0.31583189507897985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.11727144271408921 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1669 0.10444292310022141 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.10406745423347406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.25781444578918E-5 2 0.0 0.0 0.0 0.0 6.25781444578918E-5 3 0.0 0.0 0.0 0.0 6.25781444578918E-5 4 0.0 0.0 0.0 0.0 1.8773443337367538E-4 5 0.0 0.0 0.0 0.0 2.503125778315672E-4 6 0.0 0.0 0.0 0.0 2.503125778315672E-4 7 0.0 0.0 0.0 0.0 2.503125778315672E-4 8 0.0 0.0 0.0 0.0 2.503125778315672E-4 9 0.0 0.0 0.0 0.0 3.1289072228945895E-4 10-11 0.0 0.0 0.0 0.0 3.1289072228945895E-4 12-13 0.0 0.0 0.0 6.25781444578918E-5 3.1289072228945895E-4 14-15 0.0 0.0 0.0 6.25781444578918E-5 3.1289072228945895E-4 16-17 0.0 0.0 0.0 6.25781444578918E-5 3.1289072228945895E-4 18-19 0.0 0.0 0.0 6.25781444578918E-5 3.1289072228945895E-4 20-21 0.0 0.0 0.0 3.4417979451840486E-4 3.1289072228945895E-4 22-23 0.0 0.0 0.0 9.699612390973228E-4 3.7546886674735076E-4 24-25 0.0 0.0 0.0 0.0018460552615078077 4.380470112052425E-4 26-27 0.0 0.0 0.0 0.0034417979451840486 4.380470112052425E-4 28-29 0.0 0.0 0.0 0.010262815691094255 4.380470112052425E-4 30-31 0.0 0.0 0.0 0.03379219800726157 4.380470112052425E-4 32-33 0.0 0.0 0.0 0.08463694037929864 4.380470112052425E-4 34-35 0.0 0.0 0.0 0.16570692652449748 4.380470112052425E-4 36-37 0.0 0.0 0.0 0.2979971239084807 4.380470112052425E-4 38-39 0.0 0.0 0.0 0.49427347400065835 4.380470112052425E-4 40-41 0.0 0.0 0.0 0.7059440476294773 4.380470112052425E-4 42-43 0.0 0.0 0.0 0.909698485984373 4.380470112052425E-4 44-45 0.0 0.0 0.0 1.1503427404971958 4.693360834341885E-4 46-47 0.0 0.0 0.0 1.427845522095717 5.006251556631344E-4 48-49 0.0 0.0 0.0 1.694459706558565 5.006251556631344E-4 50-51 0.0 0.0 0.0 1.9584768980264107 5.006251556631344E-4 52-53 0.0 6.25781444578918E-5 0.0 2.2072563113187593 5.006251556631344E-4 54-55 0.0 6.25781444578918E-5 0.0 2.4667052982411786 5.006251556631344E-4 56-57 0.0 9.386721668683769E-5 0.0 2.6947400566457365 5.319142278920803E-4 58-59 0.0 1.251562889157836E-4 0.0 2.859727334508968 5.632033001210261E-4 60-61 0.0 1.5644536114472948E-4 3.12890722289459E-5 2.9856971393027045 5.632033001210261E-4 62-63 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.0902026405473837 5.632033001210261E-4 64-65 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.195771970247847 5.632033001210261E-4 66-67 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.284288755583535 5.632033001210261E-4 68-69 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.3875114048668276 5.632033001210261E-4 70-71 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.5759341978295396 5.632033001210261E-4 72-73 0.0 1.8773443337367538E-4 6.25781444578918E-5 3.8009339162278897 5.632033001210261E-4 74-75 0.0 1.8773443337367538E-4 6.25781444578918E-5 4.059100051188922 5.632033001210261E-4 76-77 0.0 1.8773443337367538E-4 6.25781444578918E-5 4.438792942687181 5.632033001210261E-4 78-79 0.0 1.8773443337367538E-4 6.25781444578918E-5 4.991983739694944 5.632033001210261E-4 80-81 0.0 1.8773443337367538E-4 6.25781444578918E-5 5.610756432094577 6.257814445789179E-4 82-83 0.0 2.190235056026213E-4 6.25781444578918E-5 6.1681399647810204 7.509377334947015E-4 84-85 0.0 2.503125778315672E-4 6.25781444578918E-5 6.6145411582713916 7.509377334947015E-4 86-87 0.0 2.503125778315672E-4 6.25781444578918E-5 7.017888588374733 7.509377334947015E-4 88-89 0.0 2.503125778315672E-4 6.25781444578918E-5 7.4367241092314025 7.509377334947015E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4480 0.0 52.8659 1 GTATCAA 7675 0.0 37.80477 1 TATCAAC 9875 0.0 29.327316 2 ATCAACG 9835 0.0 29.253843 3 TCAACGC 10010 0.0 28.74061 4 CAACGCA 10105 0.0 28.470411 5 AACGCAG 10420 0.0 27.720352 6 GTACATG 8505 0.0 27.45875 1 TACATGG 8465 0.0 27.358707 2 ACATGGG 8525 0.0 26.003448 3 ACGCAGA 11795 0.0 24.368248 7 CGCAGAG 11845 0.0 24.225603 8 GTGGTAT 2230 0.0 24.118475 1 TGGTATC 2170 0.0 23.474972 2 TACCTGG 1225 0.0 23.102352 2 GAGTACT 7310 0.0 22.367533 12-13 GCAGAGT 12660 0.0 22.256653 9 CATGGGA 4830 0.0 22.165573 4 CATGGGG 3815 0.0 22.005219 4 GTACTTT 7700 0.0 21.204697 14-15 >>END_MODULE