FastQCFastQC Report
Fri 27 May 2016
SRR939846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659813
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81240.4894527275060504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64880.39088740719587084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42790.25780012567680816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.051.4244881
GTATCAA78400.038.702881
TATCAAC99900.030.4676132
ATCAACG99450.030.4162623
TCAACGC101200.029.7973184
CAACGCA103000.029.5058465
AACGCAG105600.028.8239236
GTACATG87400.027.0742361
TACATGG88950.026.4966772
ACGCAGA120400.025.2026377
CGCAGAG119950.025.1410648
ACATGGG92100.024.7221013
GAGTACT68200.023.00579812-13
GCAGAGT124600.022.8812879
CATGGGG36850.021.7025664
GTACTTT73550.021.49454714-15
CATGGGA53650.021.0446224
CAGAGTA122700.020.64716510-11
TACCTGG12950.020.3431382
AGTACTT72200.019.5157612-13
Produced by FastQC (version 0.11.5)