FastQCFastQC Report
Fri 27 May 2016
SRR939846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659813
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88510.5332528423382634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78130.4707156770069882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52770.3179273809760497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.1124825507451743No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17810.10730124417630178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.10507207739667059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.050.72681
GTATCAA79100.037.3268051
ATCAACG99100.029.5372313
TATCAAC99300.029.5344122
TCAACGC100100.029.2421534
CAACGCA102100.028.6693425
AACGCAG104750.027.9893446
GTACATG85450.025.1092221
GAGTACG1551.4194011E-624.4999491
CGTACAC1751.5047954E-724.3984783
TACATGG87350.024.3390832
ACGCAGA121050.023.867717
CGCAGAG122300.023.5849748
TGGTATC22350.022.9316522
TATAACG1050.002208550722.597992
ACATGGG88600.022.5427363
TACCTGG12000.022.5414962
GAGTACT74800.022.26197812-13
GCAGAGT129850.021.8482939
GTGGTAT23900.021.8474981
Produced by FastQC (version 0.11.5)