FastQCFastQC Report
Fri 27 May 2016
SRR939845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590850
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139960.38976843922748095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105780.29458206274280463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.19716780149546764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93550.040.724291
GTATCAA160800.037.7959141
ATCAACG201350.030.8447953
TATCAAC202150.030.815752
TCAACGC202350.030.6697734
CAACGCA206200.030.0046735
AACGCAG211950.029.3697576
GTACATG171000.026.912711
TACATGG174050.026.877242
ACGCAGA236250.025.9873737
CGCAGAG239050.025.5245598
ACATGGG177750.025.4376623
GCAGAGT246400.023.4537669
CATGGGA115250.022.7661824
GAGTACT126650.022.36391812-13
AGTACTT131800.021.05810212-13
GTACTTT136450.020.75887314-15
AGAGTAC215600.020.6745410-11
CATGGGG63700.020.4087854
CAGAGTA240150.019.4994510-11
Produced by FastQC (version 0.11.5)