##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939845_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3590850 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.770938078727877 31.0 28.0 33.0 16.0 34.0 2 29.61446844061991 31.0 30.0 33.0 25.0 34.0 3 29.864158346909505 31.0 30.0 33.0 25.0 34.0 4 33.64735146274559 35.0 33.0 37.0 28.0 37.0 5 33.61025745993288 35.0 33.0 37.0 28.0 37.0 6 33.63493406853531 35.0 33.0 37.0 28.0 37.0 7 33.55477198991882 35.0 33.0 37.0 28.0 37.0 8 33.4202924098751 35.0 33.0 37.0 27.0 37.0 9 34.6601968893159 37.0 34.0 39.0 27.0 39.0 10-11 34.49841388529178 37.0 33.5 39.0 27.0 39.0 12-13 34.37000111394238 37.0 33.0 39.0 27.0 39.0 14-15 35.00717253575059 38.0 33.0 40.0 25.5 41.0 16-17 34.84771864600304 38.0 33.0 40.0 25.5 41.0 18-19 34.716147012545775 38.0 33.0 40.0 25.0 41.0 20-21 34.90698441873094 38.0 33.0 40.0 25.0 41.0 22-23 34.79567595416127 38.0 33.0 40.0 25.0 41.0 24-25 34.57222315050754 38.0 33.0 40.0 24.5 41.0 26-27 34.470913015024294 38.0 33.0 40.0 24.0 41.0 28-29 34.51461381010067 38.0 33.0 40.0 24.0 41.0 30-31 34.28314967208321 38.0 33.0 40.0 23.5 41.0 32-33 33.926085606472 38.0 32.5 40.0 22.0 41.0 34-35 33.73385048108386 38.0 32.0 40.0 21.0 41.0 36-37 33.530610440424965 38.0 32.0 40.0 19.0 41.0 38-39 33.31616831669382 37.0 31.5 40.0 18.5 41.0 40-41 33.07675230098723 37.0 31.0 40.0 17.0 41.0 42-43 32.66354484314299 37.0 31.0 40.0 15.0 41.0 44-45 32.2944269462662 37.0 30.0 40.0 12.0 41.0 46-47 31.8787470933066 37.0 30.0 39.5 9.0 41.0 48-49 31.51336814403275 36.0 30.0 39.0 8.5 40.5 50-51 30.88496609437877 35.5 28.5 38.5 7.5 40.0 52-53 31.05263238508988 36.0 29.5 39.0 6.5 40.0 54-55 31.147612960719606 36.0 29.0 39.0 2.0 40.0 56-57 30.844055864210425 36.0 28.5 39.0 2.0 40.0 58-59 30.43737903281953 35.0 28.0 39.0 2.0 40.0 60-61 30.03407688987287 35.0 28.0 38.5 2.0 40.0 62-63 29.58763287243967 34.5 27.0 38.0 2.0 40.0 64-65 29.04693679769414 34.0 26.0 38.0 2.0 40.0 66-67 28.448550482476293 34.0 26.0 37.5 2.0 39.5 68-69 27.934161967222245 33.5 25.0 37.0 2.0 39.0 70-71 27.36673322472395 33.0 23.5 36.0 2.0 39.0 72-73 26.685983541501315 32.0 22.0 36.0 2.0 38.5 74-75 26.329672640182686 32.0 21.5 35.0 2.0 38.0 76-77 25.76684615063286 31.5 20.0 35.0 2.0 37.5 78-79 25.09592199618475 31.0 18.0 35.0 2.0 37.0 80-81 24.362633499032263 31.0 11.5 34.0 2.0 36.0 82-83 23.62252781374883 30.0 7.0 34.0 2.0 35.5 84-85 22.890677694696244 29.5 2.0 34.0 2.0 35.0 86-87 22.087111686648008 29.0 2.0 34.0 2.0 35.0 88-89 21.178700168483786 29.0 2.0 34.0 2.0 35.0 90-91 19.79465321581241 26.0 2.0 33.0 2.0 34.5 92-93 18.262093236977314 24.0 2.0 32.0 2.0 34.0 94-95 17.70233649414484 22.0 2.0 32.0 2.0 34.0 96-97 17.282089059693387 21.5 2.0 32.0 2.0 34.0 98-99 16.343114582898203 10.0 2.0 32.0 2.0 34.0 100-101 14.720769455699903 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7736887597381994 1101 2 1.1337699412768316 1101 3 1.0072841768277137 1101 4 0.8515778146722397 1101 5 0.9437064671943105 1101 6 1.008132872396196 1101 7 0.7410387049461775 1101 8 0.9378299792588791 1101 9 0.9883658507343611 1101 10-11 1.172596246818948 1101 12-13 1.1854957250704459 1101 14-15 1.47106419914482 1101 16-17 1.2498466221484748 1101 18-19 1.1869212280461383 1101 20-21 1.2609896074588036 1101 22-23 1.2026919993393435 1101 24-25 1.4750733349795198 1101 26-27 1.35300673889693 1101 28-29 1.1821328302203327 1101 30-31 1.352662558042205 1101 32-33 1.390595231970643 1101 34-35 1.390842393709022 1101 36-37 1.350093428395084 1101 38-39 1.33691685549676 1101 40-41 1.1889050594179835 1101 42-43 1.2308684411281945 1101 44-45 1.229366017034316 1101 46-47 0.9805779591053607 1101 48-49 1.092435336023346 1101 50-51 1.0602151020855999 1101 52-53 1.0557015729771884 1101 54-55 1.062912009639117 1101 56-57 0.9681615436153663 1101 58-59 1.0048070211351678 1101 60-61 0.9906475576166578 1101 62-63 0.8872600128026846 1101 64-65 0.9877605760395447 1101 66-67 0.9982222097623747 1101 68-69 0.5705930876995922 1101 70-71 0.7664702985260945 1101 72-73 0.7143806477130283 1101 74-75 0.5484308867378864 1101 76-77 0.430471247321325 1101 78-79 -0.0015001585504954562 1101 80-81 -0.03526730204677264 1101 82-83 -0.09555591794601526 1101 84-85 -0.2711985115398825 1101 86-87 -0.9581951044712191 1101 88-89 -4.731705616303932 1101 90-91 -8.292270403012033 1101 92-93 -10.486917718363829 1101 94-95 -8.632235023779812 1101 96-97 -5.262142810725004 1101 98-99 -3.8430882705176526 1101 100-101 -2.929167583784645 1102 1 1.739813660045133 1102 2 1.1496942470505687 1102 3 0.9786523282161674 1102 4 1.1147493834181574 1102 5 0.963549616770166 1102 6 1.0355514866021522 1102 7 0.980747963713128 1102 8 1.2092198818617774 1102 9 1.4488318100837603 1102 10-11 1.3776824057961576 1102 12-13 1.3090172466887324 1102 14-15 1.5473747098149957 1102 16-17 1.5291865936302145 1102 18-19 1.4912630121290746 1102 20-21 1.3667089684564573 1102 22-23 1.4038883161336955 1102 24-25 1.521486801398062 1102 26-27 1.5656955515223032 1102 28-29 1.2723079274965983 1102 30-31 1.4859624589533453 1102 32-33 1.6832650727034135 1102 34-35 1.6003023593904402 1102 36-37 1.638253517333382 1102 38-39 1.426022518062922 1102 40-41 1.5198322653536422 1102 42-43 1.605579029136024 1102 44-45 1.4980297674270489 1102 46-47 1.4937126716268097 1102 48-49 1.3520252047910226 1102 50-51 1.5225903347027554 1102 52-53 1.44273784916928 1102 54-55 1.488686594553446 1102 56-57 1.5302890540680352 1102 58-59 1.3806522974964288 1102 60-61 1.295406239976419 1102 62-63 1.457572021889856 1102 64-65 1.2934013909850428 1102 66-67 1.4001647140521989 1102 68-69 1.3870073999986978 1102 70-71 1.3248324320785514 1102 72-73 1.158779709994004 1102 74-75 0.9722400077471427 1102 76-77 0.7702602605412316 1102 78-79 0.9558245629131186 1102 80-81 1.0410003103949244 1102 82-83 0.7616920700666476 1102 84-85 0.7668987405566909 1102 86-87 0.6931504498222303 1102 88-89 0.07707945752935785 1102 90-91 -5.4035864723259035 1102 92-93 -10.28047512948595 1102 94-95 -7.915997137997856 1102 96-97 -4.438714940168898 1102 98-99 -2.9186749491072064 1102 100-101 -2.0575311674180643 1103 1 2.114955058881833 1103 2 1.3170318683391642 1103 3 1.1108153374108518 1103 4 1.0907701141098158 1103 5 0.9952575742384653 1103 6 0.9434474117067637 1103 7 0.8887778456103987 1103 8 1.0124813367563021 1103 9 1.1373707277931473 1103 10-11 0.9956376331213406 1103 12-13 1.0012780226012623 1103 14-15 1.1693782418592704 1103 16-17 1.0252381293670751 1103 18-19 1.0538940840844546 1103 20-21 0.9333416860233612 1103 22-23 0.9890431942681417 1103 24-25 0.7869781268177363 1103 26-27 0.9712462293336372 1103 28-29 0.7670786790928759 1103 30-31 0.8921733046036806 1103 32-33 1.0039450915677364 1103 34-35 0.9924894674288538 1103 36-37 0.9653248305113138 1103 38-39 0.9166767322666871 1103 40-41 0.7146122149818765 1103 42-43 0.6974250574258178 1103 44-45 0.6468865356734383 1103 46-47 0.7851490530206959 1103 48-49 0.919771051461499 1103 50-51 0.8157836238186391 1103 52-53 0.7228107310297567 1103 54-55 0.7055602169391157 1103 56-57 0.6552814447870539 1103 58-59 0.7894024689142931 1103 60-61 0.6761771688438607 1103 62-63 0.7079748956895138 1103 64-65 0.6102158951107803 1103 66-67 0.6677298612039877 1103 68-69 0.4015686371507172 1103 70-71 0.35615139641360116 1103 72-73 0.45112611122219093 1103 74-75 0.3463090832947593 1103 76-77 0.235607260477682 1103 78-79 0.04786266283385032 1103 80-81 -0.16400617839852671 1103 82-83 -0.4776795203901436 1103 84-85 -0.36886705413654397 1103 86-87 -0.5480820697361288 1103 88-89 -1.3822246026491882 1103 90-91 -6.993974110839904 1103 92-93 -10.146059187791439 1103 94-95 -8.944425829837028 1103 96-97 -5.828382790873132 1103 98-99 -4.438953097593545 1103 100-101 -3.4240700650590217 1104 1 1.8703511374646453 1104 2 1.0471371875040774 1104 3 1.0228539894800122 1104 4 0.8613047767516591 1104 5 0.6766916655522621 1104 6 0.759368434132945 1104 7 0.7740662021465567 1104 8 0.8470107473851911 1104 9 1.0158432673602462 1104 10-11 0.9562158909033442 1104 12-13 0.7244598242047502 1104 14-15 0.8788248925210311 1104 16-17 0.8060076736991775 1104 18-19 0.9387381220414 1104 20-21 0.9733981537442986 1104 22-23 1.151673985052227 1104 24-25 0.9671274273002481 1104 26-27 0.9633816188472082 1104 28-29 0.8919009838426462 1104 30-31 0.9899056922314102 1104 32-33 0.8920432106691507 1104 34-35 0.9537628445718696 1104 36-37 0.9656676702455371 1104 38-39 0.9591213855330025 1104 40-41 0.7924645106993822 1104 42-43 0.8963505187469352 1104 44-45 0.9125885096836939 1104 46-47 0.7683534460034238 1104 48-49 0.634728346674823 1104 50-51 0.5236849211602355 1104 52-53 0.5888424576504683 1104 54-55 0.5586619725445843 1104 56-57 0.7787762646460124 1104 58-59 0.6772171766959865 1104 60-61 0.8110372975267559 1104 62-63 0.7395464018029152 1104 64-65 0.83779936843343 1104 66-67 0.8321281645978402 1104 68-69 0.5641524430150362 1104 70-71 0.49072414473104686 1104 72-73 0.427791447722182 1104 74-75 0.41872528815759225 1104 76-77 0.5673283030709051 1104 78-79 0.29629188616668856 1104 80-81 0.1990008135582535 1104 82-83 -0.22816588939983262 1104 84-85 -0.21980578708841847 1104 86-87 0.06307427419348599 1104 88-89 -0.35574988761594284 1104 90-91 -3.1979896051362786 1104 92-93 -7.937556740928379 1104 94-95 -5.672323179587192 1104 96-97 -2.9576757429206246 1104 98-99 -2.059985070938721 1104 100-101 -1.6585061494774056 1105 1 1.8308850886165615 1105 2 1.2343603961188947 1105 3 0.9262454959180282 1105 4 0.7842369610579141 1105 5 0.7910729086956323 1105 6 0.79055925057731 1105 7 0.6489933053280339 1105 8 0.6836764036009058 1105 9 0.7191734249905934 1105 10-11 0.7957142380942983 1105 12-13 0.692033551036559 1105 14-15 0.8870292129205879 1105 16-17 0.6637168731333176 1105 18-19 0.7736970183402647 1105 20-21 0.5980948380216802 1105 22-23 0.5924752726554416 1105 24-25 0.7420031057458871 1105 26-27 0.5537390297831806 1105 28-29 0.5755105672481449 1105 30-31 0.8043630800713615 1105 32-33 0.9274615012748413 1105 34-35 0.7359778252289573 1105 36-37 0.6788591073954962 1105 38-39 0.7003625801747191 1105 40-41 0.567716582123829 1105 42-43 0.6768717309993733 1105 44-45 0.6283869102841919 1105 46-47 0.6883167088938862 1105 48-49 0.3233373165922586 1105 50-51 0.44454297445430413 1105 52-53 0.28510974357921626 1105 54-55 0.48057324051617556 1105 56-57 0.48749014102455845 1105 58-59 0.4884745024653725 1105 60-61 0.4721919542621329 1105 62-63 0.41329795356687526 1105 64-65 0.4514565152086476 1105 66-67 0.31089840970437166 1105 68-69 0.41907261739000035 1105 70-71 0.2555777736934566 1105 72-73 0.1695328155840663 1105 74-75 0.09822991457943786 1105 76-77 0.23177035370893506 1105 78-79 0.011026426267157774 1105 80-81 -0.05575822997774438 1105 82-83 -0.4813785821072649 1105 84-85 -0.4502014147228124 1105 86-87 -0.0945048296808757 1105 88-89 -0.29978389650791115 1105 90-91 -3.2403294847482655 1105 92-93 -8.016985222653652 1105 94-95 -5.709184249799097 1105 96-97 -3.113656710355233 1105 98-99 -2.0859303838898153 1105 100-101 -1.4209628444366995 1106 1 1.6787111755730812 1106 2 1.037349526553676 1106 3 0.7903759307006375 1106 4 0.722552178449213 1106 5 0.8052033434782402 1106 6 0.7737114244903509 1106 7 0.8104063488062963 1106 8 -0.17067142248605194 1106 9 -0.29060918370505817 1106 10-11 -0.4537422836448357 1106 12-13 -0.2792979707025651 1106 14-15 0.607953125964066 1106 16-17 0.7069233948724545 1106 18-19 0.9298111487750447 1106 20-21 0.7868176641086322 1106 22-23 0.7515785335250058 1106 24-25 0.8615683231371918 1106 26-27 0.8165107689136093 1106 28-29 0.8071681759438007 1106 30-31 0.9206674278974418 1106 32-33 0.9551788925791911 1106 34-35 0.9374723904463451 1106 36-37 0.9463862813085342 1106 38-39 0.8500908410442847 1106 40-41 0.7269557125586132 1106 42-43 0.8257847744776399 1106 44-45 0.7770282146320184 1106 46-47 0.8062514915025858 1106 48-49 0.775103620940083 1106 50-51 0.7456299309760439 1106 52-53 0.9307619174922586 1106 54-55 0.9457906318205254 1106 56-57 1.0389847062419477 1106 58-59 0.8929581981175438 1106 60-61 0.6298006499143121 1106 62-63 0.7020207796538322 1106 64-65 0.6916739065129924 1106 66-67 0.7035614531826333 1106 68-69 0.6698878347813029 1106 70-71 0.6638658171717182 1106 72-73 0.6388262938449394 1106 74-75 0.5419799145794393 1106 76-77 0.5391073102306727 1106 78-79 0.5670046871367234 1106 80-81 0.31869829176138254 1106 82-83 0.005713809197086306 1106 84-85 -0.07588076254889842 1106 86-87 0.25862017031912643 1106 88-89 0.05673784262252468 1106 90-91 -3.4877479630091344 1106 92-93 -8.667936309610173 1106 94-95 -6.048722293277358 1106 96-97 -3.1575425799204506 1106 98-99 -2.0757401664985107 1106 100-101 -1.6645769748714816 1107 1 1.7117242698356137 1107 2 1.0074489887020803 1107 3 0.8171232270153155 1107 4 0.7954206096769241 1107 5 0.7263993563358895 1107 6 0.750340921495237 1107 7 0.5168683587092247 1107 8 0.5001225722739377 1107 9 0.4425535221732204 1107 10-11 0.5679455936303555 1107 12-13 0.4677948462085979 1107 14-15 0.38559300633248483 1107 16-17 0.5397379327355836 1107 18-19 0.5983992739121007 1107 20-21 0.5571494065862126 1107 22-23 0.5036367031560118 1107 24-25 0.39071903555971943 1107 26-27 0.45565498181512254 1107 28-29 0.49713727256087736 1107 30-31 0.40062913771150477 1107 32-33 0.5565174260102381 1107 34-35 0.5350848444230962 1107 36-37 0.24247059041161378 1107 38-39 0.2779910268685484 1107 40-41 0.19000240090478115 1107 42-43 0.2664433180655408 1107 44-45 0.19114602548368964 1107 46-47 0.16878777628692632 1107 48-49 0.27846212759397915 1107 50-51 0.1769758367827663 1107 52-53 0.25130274833447075 1107 54-55 0.06366590161196228 1107 56-57 0.28302794212334703 1107 58-59 0.15546057863023677 1107 60-61 -0.25835666013520253 1107 62-63 -0.22618014427363775 1107 64-65 -0.10710925475014577 1107 66-67 -0.13489114864560037 1107 68-69 -0.12406789922453498 1107 70-71 0.051968627907978515 1107 72-73 -0.17650348651309145 1107 74-75 -0.25784728184027017 1107 76-77 -0.2813054778834996 1107 78-79 -0.27045477201079393 1107 80-81 -0.314470581808866 1107 82-83 -0.6496726417903211 1107 84-85 -0.4707037966782366 1107 86-87 -0.06355080675870184 1107 88-89 -0.310461577975115 1107 90-91 -3.4248900204646127 1107 92-93 -4.749610040869047 1107 94-95 -3.2226665719675864 1107 96-97 -1.8454206486177185 1107 98-99 -1.1144754361745317 1107 100-101 -0.8490925693937044 1108 1 1.622988410056145 1108 2 0.9061168716045529 1108 3 -0.3968223204777246 1108 4 -0.31865663393790555 1108 5 0.28643627374562897 1108 6 0.4634951895227317 1108 7 0.32951674041899537 1108 8 0.40258581287802997 1108 9 0.6906669715303906 1108 10-11 0.40797622545168366 1108 12-13 0.37436789203475485 1108 14-15 0.36377596967138714 1108 16-17 0.4832524938108165 1108 18-19 0.463455867069392 1108 20-21 0.2782800446205016 1108 22-23 0.14956802187354157 1108 24-25 0.18502695381425127 1108 26-27 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1.505770745014889 2311 84-85 1.6623645790660078 2311 86-87 1.9617581102777173 2311 88-89 2.318709892311965 2311 90-91 3.087578123947388 2311 92-93 3.8271956779674667 2311 94-95 3.49494489098765 2311 96-97 2.802889614696525 2311 98-99 2.6600310550335795 2311 100-101 2.6119719381719992 2312 1 1.2739846870558544 2312 2 0.5455454271156519 2312 3 -0.10529086188497772 2312 4 -0.7724665412138023 2312 5 -0.09925360517787851 2312 6 -0.27525368992339594 2312 7 0.0598523916739353 2312 8 0.14773659113389215 2312 9 0.17319187815392212 2312 10-11 0.10227689010262253 2312 12-13 0.055971388042244996 2312 14-15 0.22648394290206397 2312 16-17 -0.12586780779151496 2312 18-19 -0.40434783769816107 2312 20-21 0.25214877057396734 2312 22-23 0.47502458803583636 2312 24-25 0.5093721910050917 2312 26-27 0.4420389169735941 2312 28-29 0.43431272227334716 2312 30-31 0.42541099309413966 2312 32-33 0.6532220926971704 2312 34-35 0.4104670125859968 2312 36-37 0.3912136968824669 2312 38-39 0.4599932452337683 2312 40-41 0.32721829884759046 2312 42-43 0.355390594261749 2312 44-45 0.4127321231217138 2312 46-47 0.48629551569972307 2312 48-49 0.5789186093486371 2312 50-51 0.5097405399925314 2312 52-53 0.3894531387853313 2312 54-55 0.4739813188205133 2312 56-57 0.5100314549456755 2312 58-59 0.6331446055981651 2312 60-61 0.5811552704497949 2312 62-63 0.557343593580125 2312 64-65 0.4552402610559092 2312 66-67 0.7351126560535874 2312 68-69 0.8484479990279148 2312 70-71 0.7183692415634262 2312 72-73 0.6179543223681669 2312 74-75 0.7099764384402185 2312 76-77 0.8939998759241412 2312 78-79 1.0317700639103684 2312 80-81 1.091338184695779 2312 82-83 1.055844083562402 2312 84-85 1.2566400916990546 2312 86-87 1.6170432748077026 2312 88-89 2.118906529167134 2312 90-91 2.977687026589617 2312 92-93 3.99721064039138 2312 94-95 3.509110792530368 2312 96-97 2.886898637491555 2312 98-99 2.735583871256475 2312 100-101 2.7127836496692606 2313 1 -7.35707971813526 2313 2 -2.745689669913588 2313 3 -1.5548055038549826 2313 4 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-0.01122040913715594 2313 64-65 -0.10594550652016466 2313 66-67 -5.755719509785706E-4 2313 68-69 0.008897682906599869 2313 70-71 0.1129806337252397 2313 72-73 -0.03188804345722218 2313 74-75 0.08811978736374115 2313 76-77 0.32874852945850463 2313 78-79 0.16818759710556463 2313 80-81 0.5077586315191276 2313 82-83 0.6590724377850208 2313 84-85 0.6913679013251439 2313 86-87 1.2052184013708995 2313 88-89 1.5637893892270114 2313 90-91 2.315358142931725 2313 92-93 3.2661515393817986 2313 94-95 2.95819912291298 2313 96-97 2.1768589233502205 2313 98-99 1.8742131464748066 2313 100-101 1.532152289382248 2314 1 0.6893525648594938 2314 2 0.10355516544628074 2314 3 -1.2239378942126216 2314 4 -0.2792388031319817 2314 5 0.00631314333204358 2314 6 -3.1375107002097877 2314 7 -2.5920051348906448 2314 8 -2.443459544468155 2314 9 -3.038711707153258 2314 10-11 -2.5223903251669313 2314 12-13 -1.2647058432226217 2314 14-15 -1.044105251297104 2314 16-17 -1.9702717849914464 2314 18-19 -3.0881207382000113 2314 20-21 -2.919821830789907 2314 22-23 -4.046840096595762 2314 24-25 -2.9689758822494596 2314 26-27 -1.8451263966085278 2314 28-29 -1.9222482444867595 2314 30-31 -1.9236142747866154 2314 32-33 -2.1377861654879204 2314 34-35 -1.5276830664717806 2314 36-37 -1.9054899284228526 2314 38-39 -3.3337595023135123 2314 40-41 -2.695392884410367 2314 42-43 -1.638467695176125 2314 44-45 -2.13460753524701 2314 46-47 -1.7084706177541769 2314 48-49 -2.2053284912509206 2314 50-51 -3.0495248052510355 2314 52-53 -1.9616589544924175 2314 54-55 -2.5376902930319893 2314 56-57 -1.7487681790961105 2314 58-59 -0.9810061302498845 2314 60-61 -1.459555129513049 2314 62-63 -1.1942080114384268 2314 64-65 -1.6165717963666815 2314 66-67 -0.8475880168520611 2314 68-69 -0.8343700979679269 2314 70-71 -0.9015030157720965 2314 72-73 -1.9616935045674815 2314 74-75 -2.2475289151727473 2314 76-77 -1.2093685247413752 2314 78-79 -2.1776691486898585 2314 80-81 -1.157737266877131 2314 82-83 -0.7059895698143315 2314 84-85 -2.0246629233865434 2314 86-87 -0.6437075652181505 2314 88-89 0.4761571200782768 2314 90-91 1.5885235418967127 2314 92-93 2.6782152645849706 2314 94-95 2.4013507410378274 2314 96-97 1.6062767351141929 2314 98-99 1.6183876171706313 2314 100-101 1.6419814101113754 2315 1 -2.9672573233380852 2315 2 -2.1519213034273363 2315 3 -2.8489053585215167 2315 4 -4.175392647712833 2315 5 -4.039361270495309 2315 6 -3.227050558045395 2315 7 -5.633398830395372 2315 8 -3.023898902935315 2315 9 -2.6453646520108762 2315 10-11 -4.14530056977749 2315 12-13 -3.143762229598291 2315 14-15 -2.3298230717531325 2315 16-17 -2.6362544645231694 2315 18-19 -2.206864515520863 2315 20-21 -1.5507322184260417 2315 22-23 -1.029926678663493 2315 24-25 -2.549299733535804 2315 26-27 -4.011525562199147 2315 28-29 -3.390799647139474 2315 30-31 -3.3625217556381024 2315 32-33 -4.123202492921621 2315 34-35 -3.9120264040590698 2315 36-37 -3.1825705635943287 2315 38-39 -1.9140098251289146 2315 40-41 -1.522701177399444 2315 42-43 -3.144829149454182 2315 44-45 -6.432102501745547 2315 46-47 -3.308922252583919 2315 48-49 -2.244225023166802 2315 50-51 -3.47141732467999 2315 52-53 -3.3798820030067773 2315 54-55 -1.831081103018942 2315 56-57 -1.32674706517804 2315 58-59 -2.690614826158619 2315 60-61 -2.9812433700816108 2315 62-63 -4.889724130182145 2315 64-65 -4.264789535757355 2315 66-67 -3.688151240849699 2315 68-69 -2.1367476821197577 2315 70-71 -3.7111146387975573 2315 72-73 -2.688314766453928 2315 74-75 -1.7939412428562775 2315 76-77 -3.4434531455240567 2315 78-79 -1.820927762256641 2315 80-81 -1.5599509441776434 2315 82-83 -1.3036642414149178 2315 84-85 -2.7105838541933025 2315 86-87 -2.290249661743008 2315 88-89 -1.8642490270070553 2315 90-91 -0.7254202723023049 2315 92-93 -0.18026299462074036 2315 94-95 -0.19580517069505277 2315 96-97 -0.4796221050172633 2315 98-99 -0.8960792336414745 2315 100-101 -1.2265353403870964 2316 1 -7.427531991528088 2316 2 -6.041900536519236 2316 3 -3.4758727027195953 2316 4 -4.080411722820653 2316 5 -2.189443343091437 2316 6 -3.0456924313670157 2316 7 -3.3108765542296155 2316 8 -2.588433805801163 2316 9 -7.321571676346554 2316 10-11 -6.920763297436778 2316 12-13 -6.237150022674648 2316 14-15 -4.927591350951246 2316 16-17 -4.968126800653579 2316 18-19 -4.1355413236831176 2316 20-21 -2.8720262094091566 2316 22-23 -3.598330799163584 2316 24-25 -3.343629673246628 2316 26-27 -4.106663795883293 2316 28-29 -4.311289060621668 2316 30-31 -6.974380297272852 2316 32-33 -5.018426933255476 2316 34-35 -5.752096663880458 2316 36-37 -4.363653086700886 2316 38-39 -6.581799874623023 2316 40-41 -4.073425090149318 2316 42-43 -4.755936432737904 2316 44-45 -3.2520625893373705 2316 46-47 -4.378430726141012 2316 48-49 -5.438875176049233 2316 50-51 -4.920186915798826 2316 52-53 -6.427959226650369 2316 54-55 -5.348521768314033 2316 56-57 -4.681636982430156 2316 58-59 -3.3198314116713696 2316 60-61 -3.300847529380956 2316 62-63 -4.508385725265857 2316 64-65 -3.1486804055717776 2316 66-67 -4.25426090195991 2316 68-69 -3.394582668120048 2316 70-71 -2.746200882433026 2316 72-73 -2.313578886543592 2316 74-75 -2.3588050803747294 2316 76-77 -3.1336002522115116 2316 78-79 -5.025003974876565 2316 80-81 -5.905876228884484 2316 82-83 -3.650036337973045 2316 84-85 -2.2490936967007755 2316 86-87 -2.3838875561126294 2316 88-89 -1.2844256423661236 2316 90-91 -1.1648976981029016 2316 92-93 -1.1513833493881585 2316 94-95 -1.7531694592011675 2316 96-97 -3.4247462423542245 2316 98-99 -4.970392634751812 2316 100-101 -4.220266939971136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31429.0 3 10702.0 4 9883.0 5 10969.0 6 12485.0 7 14351.0 8 17280.0 9 20875.0 10 24435.0 11 29036.0 12 33429.0 13 38253.0 14 42349.0 15 47314.0 16 52266.0 17 56380.0 18 51579.0 19 49628.0 20 53680.0 21 57978.0 22 64123.0 23 72331.0 24 80831.0 25 90612.0 26 102343.0 27 115775.0 28 130562.0 29 148058.0 30 168069.0 31 189865.0 32 215126.0 33 241832.0 34 276209.0 35 319410.0 36 347983.0 37 280736.0 38 81360.0 39 1324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42978836968892 19.62992512666892 13.381938206704383 22.558348296937776 2 15.663788101304013 20.406185267481842 40.462715603114326 23.46731102809982 3 19.300663771843627 25.17885366346065 29.79424857480344 25.72623398989229 4 12.522456897537424 16.738190363097566 37.31160887042572 33.42774386893929 5 13.461380806876985 37.80900903513503 34.07003247428631 14.659577683701677 6 31.373280140335808 36.244566937698096 18.022302572590732 14.359850349375364 7 28.33264427189081 33.21156194885546 20.748867512175746 17.706926267077982 8 26.281078412366792 34.92475073812755 19.982594554940817 18.811576294564844 9 27.17164554250061 16.517020496680594 20.095473010407073 36.215860950411724 10-11 25.036082060116872 26.791912932189714 27.40738612462774 20.764618883065673 12-13 26.125754861361557 24.324435515294425 27.798547999713268 21.75126162363075 14-15 22.920406424477964 25.34814178637262 26.494869904195724 25.236581884953697 16-17 22.164490536819603 28.870105520820783 26.795864962803297 22.169538979556318 18-19 21.880515624306952 27.22643967994001 29.602661643098465 21.290383052654573 20-21 23.032535611128804 26.300575656116713 29.472591646548903 21.194297086205584 22-23 22.52584934560853 26.464338096421525 29.26029128314813 21.749521274821813 24-25 22.218577910970374 26.483636329124664 29.587868407301375 21.709917352603583 26-27 22.009000277917327 26.86209842795946 29.421380594447154 21.70752069967606 28-29 21.768058673809435 27.03826094603073 29.601185312564777 21.592495067595056 30-31 21.949266231696267 26.707720324491564 29.524732404997007 21.81828103881516 32-33 21.810916065341583 26.76363607942139 29.41558891988425 22.00985893535278 34-35 22.35256453912428 26.858706124950736 29.170955691489418 21.617773644435562 36-37 22.125796858400236 26.65970412889748 29.20099784899174 22.01350116371054 38-39 21.87219287104898 26.932571131609716 29.43045451189919 21.764781485442107 40-41 22.106399588922223 26.692897715933757 29.405542125563493 21.795160569580524 42-43 21.962246762900847 26.65278735416849 29.254481646193074 22.130484236737587 44-45 22.024482282661413 26.94718538028636 29.051333872497224 21.976998464555 46-47 22.04920142170438 27.02218021839139 28.5896807528982 22.33893760700603 48-49 22.10482140371593 27.099801740401247 28.539288446146504 22.256088409736318 50-51 21.92837811707925 27.39006150566367 28.353084811640212 22.328475565616863 52-53 22.181234748950875 27.390165221618563 28.141021542111254 22.28757848731931 54-55 21.768880430293798 27.18688143382122 28.421982444133764 22.62225569175122 56-57 21.932124649277004 27.617166116313157 28.217725598179687 22.23298363623015 58-59 22.06450766426312 27.147501123608052 28.203190209395874 22.58480100273295 60-61 21.97328539219481 27.137264904837266 28.518609889311 22.370839813656918 62-63 21.90691101099622 27.116689751320582 28.400012334139657 22.57638690354354 64-65 21.716767576363175 26.997535499316378 28.39748574327585 22.888211181044596 66-67 21.73711621227253 27.287677028545183 28.082410360864525 22.892796398317767 68-69 21.78458616300914 27.070629182593297 28.277390068760617 22.867394585636948 70-71 21.617806327262894 27.22302438572394 28.184378618195804 22.974790668817366 72-73 21.84424196283023 27.000836709345265 28.168642300194115 22.986279027630392 74-75 21.71810136325654 27.163924416583768 28.006114817977934 23.111859402181757 76-77 21.79225776366731 27.07424611136009 28.07294349901501 23.060552625957587 78-79 21.819390608325033 27.172886588323596 27.94559972211534 23.062123081236027 80-81 21.64755015683939 27.268229917541507 27.996972821719723 23.08724710389938 82-83 21.949153003858367 27.14393607563644 27.738504035066967 23.168406885438223 84-85 21.85837500966701 26.93589396747808 27.76674040118165 23.438990621673256 86-87 21.778774461094404 26.783036454914583 28.13946170724891 23.298727376742107 88-89 21.85653515995224 26.771036646527826 27.879117260149822 23.493310933370115 90-91 21.656597319739202 26.844665381400638 28.262015241927273 23.236722056932884 92-93 22.015293590802944 26.531190551597135 28.228204545998924 23.225311311600993 94-95 21.79897821721638 26.4966135961693 28.102729369806532 23.601678816807787 96-97 22.143191106185494 26.42606530710741 27.718429572759955 23.71231401394714 98-99 22.058766214309035 26.391685265588695 27.557714397947414 23.991834122154863 100-101 22.2035257406678 26.372411091150916 27.77063250198651 23.653430666194776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5250.0 1 3155.5 2 1110.5 3 1169.0 4 1034.5 5 990.5 6 1420.0 7 2569.0 8 4036.0 9 4731.5 10 4360.0 11 3861.5 12 3919.5 13 4162.5 14 4467.0 15 4725.5 16 4974.0 17 5470.0 18 5892.5 19 6333.5 20 7074.5 21 8107.0 22 9536.5 23 11378.0 24 13526.0 25 16475.5 26 20685.0 27 25795.5 28 30582.5 29 35357.5 30 40450.5 31 45998.0 32 52281.0 33 58997.0 34 66636.0 35 74028.0 36 81368.5 37 88879.5 38 94835.5 39 98703.5 40 102546.0 41 109584.0 42 117916.0 43 124952.0 44 131684.5 45 137438.0 46 140630.0 47 143506.0 48 149225.0 49 150658.5 50 145919.0 51 140024.5 52 132906.5 53 127000.5 54 119926.0 55 108469.0 56 95887.0 57 84011.0 58 73470.5 59 64232.0 60 54417.0 61 43956.5 62 34690.0 63 26861.0 64 20451.0 65 15565.5 66 11471.0 67 8554.0 68 6219.0 69 4127.5 70 3001.5 71 2344.5 72 1865.0 73 1499.5 74 1171.0 75 941.0 76 661.0 77 421.0 78 278.5 79 189.0 80 131.5 81 101.0 82 65.5 83 41.5 84 31.5 85 23.0 86 16.0 87 13.0 88 11.5 89 7.5 90 4.0 91 3.0 92 3.5 93 6.5 94 7.0 95 4.0 96 2.0 97 0.5 98 2.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13720985282036285 2 0.12412102983973156 3 0.1302755614965816 4 0.14180486514335047 5 0.12888313351991867 6 0.1438378099892783 7 0.174443376916329 8 0.1605469457092332 9 0.17188130943926924 10-11 0.1661445061754181 12-13 0.15564559923137974 14-15 0.16097859838199868 16-17 0.15556205355277997 18-19 0.1711850954509378 20-21 0.17632315468482393 22-23 0.21263767631619251 24-25 0.23398359719843492 26-27 0.24644582758956793 28-29 0.24151663255218125 30-31 0.23348232312683628 32-33 0.23433170419260063 34-35 0.22451508695712713 36-37 0.23236838074550595 38-39 0.21990615035437291 40-41 0.224821421111993 42-43 0.21776181127031202 44-45 0.20953256192823427 46-47 0.22579612069565702 48-49 0.26273723491652395 50-51 0.26379548017878773 52-53 0.2656474093877494 54-55 0.264087890053887 56-57 0.23969255190275282 58-59 0.24061155436735038 60-61 0.2107300499881644 62-63 0.20330840887255106 64-65 0.20511856524221286 66-67 0.21263767631619251 68-69 0.21224779648272696 70-71 0.21809599398471113 72-73 0.24955094197752622 74-75 0.25228010081178553 76-77 0.2669284431262793 78-79 0.2647841040422184 80-81 0.27730203155241795 82-83 0.27655012044502 84-85 0.25289276912151715 86-87 0.2560118077892421 88-89 0.25889413370093434 90-91 0.44485288998426553 92-93 0.593605970731164 94-95 0.34800952420736037 96-97 0.2916858125513458 98-99 0.322082515281897 100-101 0.34363730036063883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3590850.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.03571187958528 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96371491651135 38.487545589393676 2 13.062960275856824 13.594808744179069 3 5.283802517698425 8.248392763187477 4 2.6028065644467593 5.417555698633791 5 1.4928939860838775 3.8841900663313114 6 0.8869615553490423 2.7692205565446955 7 0.5981439089544133 2.1787390876216515 8 0.3973923325166754 1.654287433439525 9 0.2832841872707127 1.3266804913975156 >10 1.2855615809245196 11.528062271567325 >50 0.08550376177156468 3.0839029066925248 >100 0.05285919122859613 5.084899576308096 >500 0.0028532201655457443 1.0618698671909992 >1k 0.0010973923667445791 0.7760663808673279 >5k 5.4869618337228926E-5 0.20214671927231082 >10k+ 1.0973923667445785E-4 0.7016318473725658 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13996 0.38976843922748095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10578 0.29458206274280463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7080 0.19716780149546764 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.784855953325814E-5 0.0 4 0.0 0.0 0.0 2.784855953325814E-5 0.0 5 0.0 0.0 0.0 2.784855953325814E-5 0.0 6 0.0 0.0 0.0 2.784855953325814E-5 0.0 7 0.0 0.0 0.0 2.784855953325814E-5 0.0 8 0.0 0.0 0.0 2.784855953325814E-5 0.0 9 0.0 0.0 0.0 2.784855953325814E-5 0.0 10-11 0.0 0.0 0.0 2.784855953325814E-5 2.784855953325814E-5 12-13 0.0 0.0 0.0 8.354567859977442E-5 2.784855953325814E-5 14-15 0.0 0.0 0.0 1.1139423813303256E-4 2.784855953325814E-5 16-17 0.0 1.392427976662907E-5 0.0 1.5316707743291978E-4 2.784855953325814E-5 18-19 0.0 2.784855953325814E-5 0.0 2.2278847626606513E-4 2.784855953325814E-5 20-21 0.0 2.784855953325814E-5 0.0 4.0380411323224304E-4 2.784855953325814E-5 22-23 0.0 2.784855953325814E-5 0.0 9.050781848308897E-4 2.784855953325814E-5 24-25 0.0 2.784855953325814E-5 0.0 0.001782307810128521 2.784855953325814E-5 26-27 0.0 2.784855953325814E-5 0.0 0.003954495453722656 2.784855953325814E-5 28-29 1.392427976662907E-5 2.784855953325814E-5 0.0 0.0099140871938399 2.784855953325814E-5 30-31 2.784855953325814E-5 2.784855953325814E-5 0.0 0.030772658284250246 2.784855953325814E-5 32-33 2.784855953325814E-5 2.784855953325814E-5 0.0 0.07499617082306417 2.784855953325814E-5 34-35 2.784855953325814E-5 2.784855953325814E-5 0.0 0.14113649971455225 2.784855953325814E-5 36-37 2.784855953325814E-5 2.784855953325814E-5 0.0 0.2531851789966164 2.784855953325814E-5 38-39 2.784855953325814E-5 2.784855953325814E-5 0.0 0.42119553866076276 2.784855953325814E-5 40-41 2.784855953325814E-5 2.784855953325814E-5 0.0 0.6044251361098347 2.784855953325814E-5 42-43 2.784855953325814E-5 2.784855953325814E-5 0.0 0.7880306891126057 2.784855953325814E-5 44-45 2.784855953325814E-5 2.784855953325814E-5 0.0 1.0063633958533496 2.784855953325814E-5 46-47 2.784855953325814E-5 5.569711906651628E-5 0.0 1.2680841583469094 2.784855953325814E-5 48-49 2.784855953325814E-5 5.569711906651628E-5 0.0 1.5493546096328168 2.784855953325814E-5 50-51 2.784855953325814E-5 5.569711906651628E-5 0.0 1.8421125917261931 2.784855953325814E-5 52-53 2.784855953325814E-5 5.569711906651628E-5 0.0 2.123619755768133 2.784855953325814E-5 54-55 2.784855953325814E-5 5.569711906651628E-5 0.0 2.424133561691521 2.784855953325814E-5 56-57 2.784855953325814E-5 5.569711906651628E-5 0.0 2.770235459570854 2.784855953325814E-5 58-59 2.784855953325814E-5 5.569711906651628E-5 0.0 3.148098639597867 2.784855953325814E-5 60-61 2.784855953325814E-5 5.569711906651628E-5 0.0 3.5513318573596777 2.784855953325814E-5 62-63 2.784855953325814E-5 5.569711906651628E-5 0.0 3.9376749237645683 2.784855953325814E-5 64-65 2.784855953325814E-5 5.569711906651628E-5 0.0 4.326426890569085 2.784855953325814E-5 66-67 2.784855953325814E-5 5.569711906651628E-5 0.0 4.7583162760906195 2.784855953325814E-5 68-69 2.784855953325814E-5 5.569711906651628E-5 0.0 5.230154420262612 2.784855953325814E-5 70-71 2.784855953325814E-5 5.569711906651628E-5 0.0 5.742526142835262 2.784855953325814E-5 72-73 4.177283929988721E-5 5.569711906651628E-5 0.0 6.2421571494214465 2.784855953325814E-5 74-75 5.569711906651628E-5 5.569711906651628E-5 0.0 6.737986827631341 2.784855953325814E-5 76-77 5.569711906651628E-5 5.569711906651628E-5 0.0 7.258866285141401 2.784855953325814E-5 78-79 5.569711906651628E-5 5.569711906651628E-5 0.0 7.816255204199563 2.784855953325814E-5 80-81 5.569711906651628E-5 5.569711906651628E-5 0.0 8.39634069927733 2.784855953325814E-5 82-83 5.569711906651628E-5 5.569711906651628E-5 0.0 8.953590375537825 2.784855953325814E-5 84-85 5.569711906651628E-5 5.569711906651628E-5 0.0 9.483116810782963 2.784855953325814E-5 86-87 5.569711906651628E-5 5.569711906651628E-5 0.0 10.015469874820724 2.784855953325814E-5 88-89 5.569711906651628E-5 5.569711906651628E-5 0.0 10.581046270381666 5.569711906651628E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9355 0.0 40.72429 1 GTATCAA 16080 0.0 37.795914 1 ATCAACG 20135 0.0 30.844795 3 TATCAAC 20215 0.0 30.81575 2 TCAACGC 20235 0.0 30.669773 4 CAACGCA 20620 0.0 30.004673 5 AACGCAG 21195 0.0 29.369757 6 GTACATG 17100 0.0 26.91271 1 TACATGG 17405 0.0 26.87724 2 ACGCAGA 23625 0.0 25.987373 7 CGCAGAG 23905 0.0 25.524559 8 ACATGGG 17775 0.0 25.437662 3 GCAGAGT 24640 0.0 23.453766 9 CATGGGA 11525 0.0 22.766182 4 GAGTACT 12665 0.0 22.363918 12-13 AGTACTT 13180 0.0 21.058102 12-13 GTACTTT 13645 0.0 20.758873 14-15 AGAGTAC 21560 0.0 20.67454 10-11 CATGGGG 6370 0.0 20.408785 4 CAGAGTA 24015 0.0 19.49945 10-11 >>END_MODULE