FastQCFastQC Report
Fri 27 May 2016
SRR939845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590850
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158660.44184524555467364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129920.3618084854560898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89960.2505256415611902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.044.113891
GTATCAA161000.036.9789731
ATCAACG206100.028.6272493
TCAACGC206700.028.5433564
TATCAAC208500.028.252882
CAACGCA211450.027.9021595
AACGCAG215950.027.3028536
GTACATG176500.025.5525671
TACATGG177750.024.7028162
ACGCAGA245700.023.9394047
ACATGGG177300.023.8341463
CGCAGAG246350.023.743318
GCAGAGT260150.021.7953519
CATGGGA114850.021.55844
GAGTACT134450.021.506812-13
AGTACTT141300.019.89712512-13
AGAGTAC233800.019.85700610-11
GTACTTT149450.019.5527914-15
CAGAGTA257700.018.8292910-11
CATGGGG63200.018.7303054
Produced by FastQC (version 0.11.5)