##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939845_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3590850 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41473634376262 31.0 31.0 34.0 30.0 34.0 2 31.615890945040867 31.0 31.0 34.0 30.0 34.0 3 31.675108122032388 31.0 31.0 34.0 30.0 34.0 4 35.489282481863626 37.0 35.0 37.0 33.0 37.0 5 35.21952991631508 37.0 35.0 37.0 32.0 37.0 6 35.24582201985602 37.0 35.0 37.0 32.0 37.0 7 35.16637954801788 37.0 35.0 37.0 32.0 37.0 8 35.110212902237635 37.0 35.0 37.0 32.0 37.0 9 36.7059186543576 39.0 35.0 39.0 32.0 39.0 10-11 36.56428269072782 38.5 35.0 39.0 32.0 39.0 12-13 36.50238105184009 38.0 35.0 39.0 32.0 39.0 14-15 37.528095158527925 39.0 36.0 41.0 32.0 41.0 16-17 37.462488268794296 39.0 36.0 41.0 32.0 41.0 18-19 37.4546536335408 39.0 36.0 41.0 32.0 41.0 20-21 37.39575114527201 39.0 36.0 41.0 32.0 41.0 22-23 37.248896500828494 39.0 36.0 40.0 31.0 41.0 24-25 37.08671094587632 39.0 36.0 40.0 31.0 41.0 26-27 36.8030310372196 39.0 36.0 40.0 30.5 41.0 28-29 36.574274196917166 38.0 35.0 40.0 30.0 41.0 30-31 36.31047662809641 38.0 35.0 40.0 30.0 41.0 32-33 36.26687093585085 38.0 35.0 40.0 30.0 41.0 34-35 36.32570241029283 38.0 35.0 40.0 30.0 41.0 36-37 36.284411908044056 38.0 35.0 40.0 30.0 41.0 38-39 36.159970759012495 38.0 35.0 40.0 30.0 41.0 40-41 35.98247267360095 38.0 35.0 40.0 29.5 41.0 42-43 35.7706804795522 38.0 35.0 40.0 28.5 41.0 44-45 35.499687956890426 38.0 34.0 40.0 27.5 41.0 46-47 35.16962042413356 38.0 34.0 40.0 26.5 41.0 48-49 34.85134480693986 38.0 33.5 40.0 26.0 41.0 50-51 34.584576493030895 38.0 33.0 40.0 24.5 41.0 52-53 34.30690268320871 38.0 33.0 40.0 24.0 41.0 54-55 34.00591948981439 38.0 33.0 40.0 23.5 41.0 56-57 32.813169305317686 36.5 31.5 39.5 16.0 41.0 58-59 29.592993720149824 34.5 24.5 39.0 7.5 40.0 60-61 29.553672389545653 34.0 25.0 38.0 9.0 40.0 62-63 31.35146469498865 35.0 29.5 39.0 9.5 40.0 64-65 30.911298578331035 35.0 29.0 38.5 4.5 40.0 66-67 25.53078881044878 29.0 18.0 36.0 2.0 39.0 68-69 17.795910717518137 13.5 7.0 29.5 2.0 35.5 70-71 16.640538034170184 15.0 7.5 26.5 2.0 33.5 72-73 18.84043624768509 19.5 7.5 30.0 2.0 35.0 74-75 19.569516827492095 20.0 8.5 31.5 2.0 35.5 76-77 20.569773451968196 23.5 9.5 31.5 2.0 34.5 78-79 23.701380731581658 29.0 12.0 34.0 2.0 36.0 80-81 26.347931130512276 31.5 23.0 35.0 2.0 36.0 82-83 27.224667975548964 33.0 26.0 35.0 2.0 36.0 84-85 27.182915883425927 33.0 26.0 35.0 2.0 36.0 86-87 26.88263642313101 33.0 26.0 35.0 2.0 36.0 88-89 26.456640628263504 33.0 25.0 35.0 2.0 35.0 90-91 24.92240402690171 32.0 15.0 34.5 2.0 35.0 92-93 22.928760460615173 31.0 2.0 34.0 2.0 35.0 94-95 18.487012545776068 24.0 2.0 33.0 2.0 35.0 96-97 17.175950819443862 16.0 2.0 32.5 2.0 34.5 98-99 16.081179804224625 6.5 2.0 32.0 2.0 34.0 100-101 15.453285016082543 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40294013157760133 1101 2 0.4615059371756587 1101 3 0.352555439743643 1101 4 0.2983594467114088 1101 5 0.3659523150001007 1101 6 0.41925345773022826 1101 7 0.33071022958218066 1101 8 0.39984409503299645 1101 9 0.5749428520865791 1101 10-11 0.5808865120069555 1101 12-13 0.5844201764106103 1101 14-15 0.6879400042828436 1101 16-17 0.5770856452233133 1101 18-19 0.634246651107162 1101 20-21 0.6830359865353586 1101 22-23 0.5889313025362739 1101 24-25 0.6811286509573762 1101 26-27 0.7001800370656213 1101 28-29 0.5875200570273691 1101 30-31 0.6976033175667311 1101 32-33 0.6852402518406535 1101 34-35 0.812878767075901 1101 36-37 0.7468480362253018 1101 38-39 0.7587948894523109 1101 40-41 0.8650165996960979 1101 42-43 0.7115324154721208 1101 44-45 0.5404383429567474 1101 46-47 0.6455041955407594 1101 48-49 0.47522137130722086 1101 50-51 0.4522133807191011 1101 52-53 0.5091269093434008 1101 54-55 0.5352339637058989 1101 56-57 1.3741110499327505 1101 58-59 -8.354010575064134 1101 60-61 -9.56687219141908 1101 62-63 -2.631919320573857 1101 64-65 0.04550485864759679 1101 66-67 -0.8617412496712689 1101 68-69 -3.1292572727156234 1101 70-71 -3.990290528694395 1101 72-73 -6.463820346407893 1101 74-75 -7.483379570852417 1101 76-77 -8.33497036461921 1101 78-79 -11.517244138636965 1101 80-81 -7.43526493648525 1101 82-83 -3.339599850940971 1101 84-85 -2.2877142508794925 1101 86-87 -2.0896983143800156 1101 88-89 -2.255570020140631 1101 90-91 -7.931426375883536 1101 92-93 -13.933276036149195 1101 94-95 -12.305503362323998 1101 96-97 -12.260835636831018 1101 98-99 -11.88761246497602 1101 100-101 -11.720593574543518 1102 1 0.4928088992504627 1102 2 0.4846564797476205 1102 3 0.4345442257841903 1102 4 0.42136618958788574 1102 5 0.42517603832465056 1102 6 0.47269014961219114 1102 7 0.4727601607033307 1102 8 0.44048261401697175 1102 9 0.5933057106795161 1102 10-11 0.6195233150617838 1102 12-13 0.6353542702308488 1102 14-15 0.745561388143436 1102 16-17 0.8143007647341491 1102 18-19 0.7050879397457805 1102 20-21 0.8099591564123472 1102 22-23 0.8267548518511134 1102 24-25 0.905048944840452 1102 26-27 0.8790958598657284 1102 28-29 0.8352379921120772 1102 30-31 0.8212239280508129 1102 32-33 0.6903620586415258 1102 34-35 0.647666209374762 1102 36-37 0.7548875267635182 1102 38-39 0.7006611436515016 1102 40-41 0.6953269170221432 1102 42-43 0.792778035019218 1102 44-45 0.7664407114223195 1102 46-47 0.775326923143048 1102 48-49 0.8044846093380116 1102 50-51 0.6910598413131481 1102 52-53 0.6283096678807638 1102 54-55 0.4538542116697428 1102 56-57 1.1407948356107767 1102 58-59 -9.317712027561896 1102 60-61 -10.147679161474912 1102 62-63 -3.075734725267285 1102 64-65 -0.16027069298737118 1102 66-67 -1.0774830869238485 1102 68-69 -3.8068758772543774 1102 70-71 -4.29820434554917 1102 72-73 -6.895763213217956 1102 74-75 -7.837537678013401 1102 76-77 -8.894414016889794 1102 78-79 -12.011735039378923 1102 80-81 -8.212033431059528 1102 82-83 -3.833052131193284 1102 84-85 -2.8911974661923487 1102 86-87 -2.6763539443193523 1102 88-89 -2.641950738897428 1102 90-91 -8.283676418177565 1102 92-93 -14.468489439730895 1102 94-95 -12.55740961458975 1102 96-97 -12.482302877085264 1102 98-99 -11.84305026858672 1102 100-101 -11.635362649150183 1103 1 0.31731546664527954 1103 2 0.22950006303328863 1103 3 0.25786692284784607 1103 4 0.1888143544968912 1103 5 0.2189344210765043 1103 6 0.2050021669245652 1103 7 0.3215040440120802 1103 8 0.40412697461996316 1103 9 0.3954098859170756 1103 10-11 0.28703227060017866 1103 12-13 0.3119790607665749 1103 14-15 0.41482373942798745 1103 16-17 0.4064424679420924 1103 18-19 0.4315026806790314 1103 20-21 0.2730334062710327 1103 22-23 0.4018821191659967 1103 24-25 0.4470809406127003 1103 26-27 0.25787917764602497 1103 28-29 0.38239772346265255 1103 30-31 0.2804296529059016 1103 32-33 0.3072851831628114 1103 34-35 0.43743264625786793 1103 36-37 0.25533522006433884 1103 38-39 0.16055237274270695 1103 40-41 0.22567289681323643 1103 42-43 0.21725232568370956 1103 44-45 0.09341720125263464 1103 46-47 0.11898906103325402 1103 48-49 0.12765018729216848 1103 50-51 0.2702314832789199 1103 52-53 0.2646222437645278 1103 54-55 0.06263348231598087 1103 56-57 0.5865086363379248 1103 58-59 -6.60956031355045 1103 60-61 -8.298743290153702 1103 62-63 -2.5104420773378457 1103 64-65 -0.2277353307354275 1103 66-67 -1.6537431336832995 1103 68-69 -4.268939597875029 1103 70-71 -4.7954757075602945 1103 72-73 -7.547224696877233 1103 74-75 -8.295010995358883 1103 76-77 -9.258973498053898 1103 78-79 -12.217424607472791 1103 80-81 -8.517459600965577 1103 82-83 -4.564552129520916 1103 84-85 -3.690513717944153 1103 86-87 -3.426428197522778 1103 88-89 -3.61089726321201 1103 90-91 -9.050325524965043 1103 92-93 -14.910664673946236 1103 94-95 -12.786875844433517 1103 96-97 -12.758728321182971 1103 98-99 -12.395275356135262 1103 100-101 -12.10072570383332 1104 1 0.14633970356941717 1104 2 0.13835060593438442 1104 3 0.18760279894496534 1104 4 0.16939477916333345 1104 5 0.13336522773677473 1104 6 0.13845070985568952 1104 7 0.172650454600209 1104 8 0.2329722826715468 1104 9 0.1838676863792017 1104 10-11 0.236341549370934 1104 12-13 0.17642724541087773 1104 14-15 0.28743035121454596 1104 16-17 0.26591336196427307 1104 18-19 0.34619308438461616 1104 20-21 0.25662054398833334 1104 22-23 0.351727219110856 1104 24-25 0.2742623603296366 1104 26-27 0.29338239900936003 1104 28-29 0.286410321428356 1104 30-31 0.3614468871461085 1104 32-33 0.32036275977075945 1104 34-35 0.29488190147179694 1104 36-37 0.2124135304093926 1104 38-39 0.17805178811299527 1104 40-41 0.30566032459269366 1104 42-43 0.23671491689130875 1104 44-45 0.1380433278716282 1104 46-47 0.12439050319138545 1104 48-49 0.13053836532854746 1104 50-51 0.019574767117177316 1104 52-53 -0.04756261936775985 1104 54-55 -0.14875027720704992 1104 56-57 0.6079516740953466 1104 58-59 -6.6716175235902035 1104 60-61 -8.73410158771199 1104 62-63 -2.7066037955699294 1104 64-65 -0.43232966207994394 1104 66-67 -2.047916467274085 1104 68-69 -4.336512816041672 1104 70-71 -4.672057586828359 1104 72-73 -7.6610350451449065 1104 74-75 -8.618413347179388 1104 76-77 -9.195724716121852 1104 78-79 -12.22168736259125 1104 80-81 -8.506794345991931 1104 82-83 -4.638007258922535 1104 84-85 -3.733449781980955 1104 86-87 -3.453078516011878 1104 88-89 -3.711450998727674 1104 90-91 -9.123473750587056 1104 92-93 -14.664711384250952 1104 94-95 -12.582638512662056 1104 96-97 -12.333631723191523 1104 98-99 -11.719112479370914 1104 100-101 -11.314532512141282 1105 1 0.1373741166417659 1105 2 0.11920616918861171 1105 3 0.10977714503885139 1105 4 0.08693295977422366 1105 5 0.15238877124390626 1105 6 0.14613735458113553 1105 7 0.12765146505115865 1105 8 0.16035839041449407 1105 9 0.14295633179752798 1105 10-11 0.11232222810527048 1105 12-13 0.2514451087401639 1105 14-15 0.2939697732366042 1105 16-17 0.2696384976534105 1105 18-19 0.22601185278925584 1105 20-21 0.2727697154185549 1105 22-23 0.16802037980142615 1105 24-25 0.27306136720070384 1105 26-27 0.20970533191541563 1105 28-29 0.18948876850959806 1105 30-31 0.12566880382721024 1105 32-33 0.19899948100177767 1105 34-35 0.1547702466418457 1105 36-37 0.16228271309892506 1105 38-39 0.20694996352727912 1105 40-41 0.17618871826438465 1105 42-43 0.2412058300502693 1105 44-45 0.1459049971366042 1105 46-47 0.13246978028590917 1105 48-49 0.12635960933801726 1105 50-51 0.04676636305228499 1105 52-53 0.11355811508573055 1105 54-55 0.0559504849616701 1105 56-57 0.5137762020704102 1105 58-59 -6.6045520896736996 1105 60-61 -8.540206335387953 1105 62-63 -2.529907861913248 1105 64-65 -0.16776292901221623 1105 66-67 -1.5729799813337877 1105 68-69 -4.113203517005932 1105 70-71 -4.6109954946174945 1105 72-73 -6.222587747379446 1105 74-75 -6.682898702858122 1105 76-77 -8.58748466906371 1105 78-79 -11.972624014534205 1105 80-81 -8.02387349317133 1105 82-83 -4.059197317528689 1105 84-85 -3.087043739484276 1105 86-87 -2.8579339132634516 1105 88-89 -3.0646409562887342 1105 90-91 -8.277834026873215 1105 92-93 -11.672487886935864 1105 94-95 -11.863679024527638 1105 96-97 -12.181371200066632 1105 98-99 -11.760266883493554 1105 100-101 -11.451181748529065 1106 1 0.1498741166417652 1106 2 0.14420616918861384 1106 3 0.059777145038850676 1106 4 0.10758513368726597 1106 5 0.054019206026517 1106 6 0.0396156154507068 1106 7 0.13444494331202606 1106 8 0.14351056432753495 1106 9 0.12040198397144053 1106 10-11 0.18052874984439882 1106 12-13 0.23840163047930218 1106 14-15 0.30551868628008805 1106 16-17 0.18635045417514817 1106 18-19 0.3014194614849117 1106 20-21 0.1782044980272559 1106 22-23 0.25416168414924556 1106 24-25 0.26653962807026943 1106 26-27 0.3829390275675877 1106 28-29 0.2523963772052511 1106 30-31 0.34795141252286044 1106 32-33 0.47196143752351816 1106 34-35 0.3407756814244536 1106 36-37 0.3154077130989208 1106 38-39 0.3492053983098842 1106 40-41 0.4149115443513409 1106 42-43 0.40914061265896606 1106 44-45 0.32769847539746877 1106 46-47 0.3254045628946045 1106 48-49 0.36779982672931766 1106 50-51 0.35654897174793376 1106 52-53 0.3772809411726854 1106 54-55 0.15527113713557839 1106 56-57 1.1864664194617092 1106 58-59 -6.2457205679345655 1106 60-61 -8.57213568321404 1106 62-63 -2.0815382966958573 1106 64-65 0.20859576663995938 1106 66-67 -1.329501720464222 1106 68-69 -3.9265187343972343 1106 70-71 -4.403115059834885 1106 72-73 -5.907098616944664 1106 74-75 -6.324203050684208 1106 76-77 -8.277702060368057 1106 78-79 -11.599797927577683 1106 80-81 -7.526862623606114 1106 82-83 -3.6515886218765132 1106 84-85 -2.650223087310362 1106 86-87 -2.4243741306547584 1106 88-89 -2.654043130201778 1106 90-91 -8.083540548612348 1106 92-93 -11.188792234761952 1106 94-95 -11.625092068005898 1106 96-97 -12.432050547892718 1106 98-99 -11.953473405232684 1106 100-101 -11.619660009398629 1107 1 0.021525095970261532 1107 2 0.08111955315232677 1107 3 0.04536696394675843 1107 4 0.028068146925797066 1107 5 -0.013642106169790225 1107 6 0.012400728734249356 1107 7 0.08113494862129045 1107 8 -0.028182831408898323 1107 9 -0.013867732444573733 1107 10-11 0.12837853372269592 1107 12-13 0.10556220543803363 1107 14-15 0.05561964165917743 1107 16-17 0.08392608234194654 1107 18-19 0.058908879023945815 1107 20-21 0.06962556311191292 1107 22-23 0.06680422636367922 1107 24-25 0.03990154679234337 1107 26-27 0.16521163810994466 1107 28-29 0.12292664001385134 1107 30-31 0.03905374138564355 1107 32-33 0.02163927480609118 1107 34-35 -0.1664534667271198 1107 36-37 -0.209167185043988 1107 38-39 -0.05934789199147161 1107 40-41 -0.2135017140944342 1107 42-43 -0.13522734679441584 1107 44-45 -0.036196937859529044 1107 46-47 -0.25407094523665563 1107 48-49 -0.21736445750101296 1107 50-51 -0.21040424612812814 1107 52-53 -0.20447434068001513 1107 54-55 -0.2617707728723744 1107 56-57 0.3643489678510434 1107 58-59 -6.718047360388486 1107 60-61 -9.430788197612536 1107 62-63 -3.0173315773020377 1107 64-65 -0.5216071684600507 1107 66-67 -1.787970286154831 1107 68-69 -4.165793586949722 1107 70-71 -4.652664457637311 1107 72-73 -6.173360259815823 1107 74-75 -6.902421179226122 1107 76-77 -9.165223448913896 1107 78-79 -12.520204223384518 1107 80-81 -8.433913125100787 1107 82-83 -4.437913694590357 1107 84-85 -3.540376962207926 1107 86-87 -3.2816463083766187 1107 88-89 -3.383030885710138 1107 90-91 -8.55704987877936 1107 92-93 -11.557563442965918 1107 94-95 -11.879985572524545 1107 96-97 -12.691868539663357 1107 98-99 -12.0132663655517 1107 100-101 -11.425942002046453 1108 1 -0.020747772261806574 1108 2 0.029073025274247044 1108 3 -0.060096979449056676 1108 4 -0.03198168054999684 1108 5 -0.202263281529099 1108 6 0.007759089306482281 1108 7 -0.10232683986932756 1108 8 -0.10658579706623783 1108 9 -0.03853429636863126 1108 10-11 -0.005615445345242165 1108 12-13 -0.17553511790183052 1108 14-15 -0.04144028579163006 1108 16-17 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88-89 1.6013476993626128 2312 90-91 3.1312708511061444 2312 92-93 5.062266066574171 2312 94-95 9.040858359169828 2312 96-97 9.592999291431251 2312 98-99 10.002159538010643 2312 100-101 9.185195750875836 2313 1 -0.3266438565738099 2313 2 -0.31651766028250705 2313 3 -0.355263041915606 2313 4 -0.37978215650485225 2313 5 -0.39804263235899384 2313 6 -0.3469307826838772 2313 7 -0.34417097980140454 2313 8 -0.29862718561572166 2313 9 -0.3549702225451341 2313 10-11 -0.41040848576774636 2313 12-13 -0.4244773469458494 2313 14-15 -0.5912114807614515 2313 16-17 -0.5707253387304903 2313 18-19 -0.5647135501120459 2313 20-21 -0.5835706923320032 2313 22-23 -0.5237955907728491 2313 24-25 -0.586190402263405 2313 26-27 -0.699064929222402 2313 28-29 -0.6136275905213822 2313 30-31 -0.7082358437244238 2313 32-33 -0.5908645978451261 2313 34-35 -0.6052485055506409 2313 36-37 -0.7809959671800613 2313 38-39 -1.050268469852135 2313 40-41 -1.092517947920797 2313 42-43 -0.9729222187931299 2313 44-45 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2314 68-69 -0.24812826913969133 2314 70-71 2.530013723793072 2314 72-73 8.093106522612008 2314 74-75 9.609181192178376 2314 76-77 8.471621873523983 2314 78-79 6.19970019476477 2314 80-81 3.1741137840579228 2314 82-83 1.6064428041136622 2314 84-85 1.2392986119246778 2314 86-87 1.1344881169746834 2314 88-89 1.1009599777783308 2314 90-91 2.418460430755296 2314 92-93 4.575975689853767 2314 94-95 8.505365332718913 2314 96-97 9.279325399301218 2314 98-99 9.551479318722057 2314 100-101 8.927211129756513 2315 1 -0.8014605093464979 2315 2 -0.5788638326350508 2315 3 -0.5295312309360014 2315 4 -0.35030643080066426 2315 5 -0.6477436454308076 2315 6 -0.7395557444651928 2315 7 -0.6947078447461976 2315 8 -0.8186115620630119 2315 9 -1.038235069633508 2315 10-11 -0.9046211726499394 2315 12-13 -0.7025757654914813 2315 14-15 -0.834394414858096 2315 16-17 -0.9870192451350803 2315 18-19 -0.9131808681152833 2315 20-21 -0.8920479451442063 2315 22-23 -0.88178069347763 2315 24-25 -0.8776139243171244 2315 26-27 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4.400965016978574 2315 94-95 8.349678800862037 2315 96-97 8.947186282849941 2315 98-99 9.18091533928292 2315 100-101 8.496137366940959 2316 1 -0.5249040769500262 2316 2 -0.5018402104262201 2316 3 -0.6514550894241857 2316 4 -0.4525858990265519 2316 5 -0.6149578564577141 2316 6 -0.6296867981394101 2316 7 -0.5529462559142786 2316 8 -0.6210119737264534 2316 9 -0.7295170724577886 2316 10-11 -0.6113939753258961 2316 12-13 -0.6963281546523206 2316 14-15 -0.9241484464253276 2316 16-17 -1.039042700311441 2316 18-19 -1.038907519004539 2316 20-21 -0.8735951824033279 2316 22-23 -1.016276041862021 2316 24-25 -2.373416010648093 2316 26-27 -1.5301722540739888 2316 28-29 -1.3174053636071292 2316 30-31 -0.8751098838357336 2316 32-33 -0.752545542545441 2316 34-35 -0.8603372926736057 2316 36-37 -0.8627895579464067 2316 38-39 -1.2230706402907359 2316 40-41 -0.9276014381564366 2316 42-43 -1.0832215204669282 2316 44-45 -1.0762582986333058 2316 46-47 -1.0623172435136041 2316 48-49 -1.1930261130570727 2316 50-51 -1.1498860421281023 2316 52-53 -1.0500498657401067 2316 54-55 -1.0438886791120012 2316 56-57 0.18252042038677985 2316 58-59 3.632378677292916 2316 60-61 3.640566150105279 2316 62-63 1.0472904918837393 2316 64-65 0.6655421967131225 2316 66-67 5.760909094437803 2316 68-69 8.61015711288222 2316 70-71 5.387541476621259 2316 72-73 9.21363975661517 2316 74-75 9.991519712329836 2316 76-77 8.619587118422626 2316 78-79 5.990736667914707 2316 80-81 2.9079977750146924 2316 82-83 1.4129068473859547 2316 84-85 1.1143223146743324 2316 86-87 0.9654758609355234 2316 88-89 1.0804769374964849 2316 90-91 2.605086091283372 2316 92-93 4.413305465179182 2316 94-95 8.587820271158176 2316 96-97 9.340888281894518 2316 98-99 9.52504580046911 2316 100-101 8.598836384933216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 24.0 4 67.0 5 161.0 6 363.0 7 988.0 8 2196.0 9 3837.0 10 6278.0 11 9511.0 12 13533.0 13 18230.0 14 24017.0 15 33059.0 16 44166.0 17 56878.0 18 60031.0 19 58132.0 20 59907.0 21 59072.0 22 56861.0 23 55408.0 24 58101.0 25 64258.0 26 85753.0 27 122286.0 28 169081.0 29 219618.0 30 264156.0 31 290577.0 32 303565.0 33 317847.0 34 324808.0 35 315681.0 36 268124.0 37 167742.0 38 54198.0 39 2328.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.52451328572455 19.706057835811542 14.497670534531965 23.271758343931943 2 15.753488559918566 21.24734554741253 39.43879476364406 23.560371129024844 3 19.07595665380666 25.322003754679773 30.435826362352497 25.166213229161066 4 12.919670830026316 17.46990266928443 36.94660038709498 32.663826113594276 5 13.246673408699564 38.16991985080953 34.25846323450932 14.32494350598158 6 31.510737432846657 36.7771799928095 17.505358760560526 14.206723813783315 7 27.981898436303382 33.32119136137683 20.770708885082918 17.926201317236867 8 26.13460063021378 35.531525309502356 19.713911349556998 18.619962710726863 9 26.615091134411074 16.410153584805826 20.021331996602477 36.95342328418062 10-11 24.936741997020203 27.00715707980005 27.3706086302686 20.68549229291115 12-13 25.806786693958255 24.337691632900288 27.972443850341843 21.883077822799617 14-15 22.49623869607202 25.56912479452942 26.48432784228214 25.450308667116424 16-17 21.905133881949958 29.05630978737625 26.88245123021012 22.156105100463677 18-19 21.67475701455511 27.327171941974516 29.620058363014497 21.378012680455882 20-21 22.719537156940557 26.43400587604606 29.539231658242475 21.307225308770906 22-23 22.218583343776544 26.596822479357257 29.31624824205968 21.86834593480652 24-25 22.02762577105699 26.68040993079633 29.53802024590278 21.7539440522439 26-27 21.85593285594358 26.995445224406 29.42200397594272 21.7266179437077 28-29 21.626147490969206 27.13560799928929 29.576631078189656 21.66161343155185 30-31 21.80019615453011 26.820713283377014 29.54497190043883 21.834118661654045 32-33 21.642759513383513 26.868979814171407 29.405352959589354 22.082907712855725 34-35 22.173016370538072 26.968786016416068 29.190902722379807 21.66729489066605 36-37 22.030482205714012 26.72920826740239 29.2251897355673 22.015119791316295 38-39 21.75849175278279 26.98771590892789 29.46309974586128 21.79069259242804 40-41 21.96897429715553 26.69256714925745 29.502245818087413 21.836212735499608 42-43 21.834865375385355 26.720584110125404 29.22489415357822 22.21965636091102 44-45 21.937845732486277 26.978081486594675 29.02948096322902 22.054591817690024 46-47 21.873699871900605 27.10006336024345 28.66271722542026 22.363519542435697 48-49 22.016271382287506 27.166029746229558 28.538599439885154 22.279099431597782 50-51 21.775126814967628 27.36486011251612 28.44993745777303 22.410075614743224 52-53 22.114820295941865 27.340110866085233 28.254710109504007 22.290358728468895 54-55 21.700870004569296 27.107944712213026 28.5031049906156 22.688080292602073 56-57 21.84405786114157 27.42924706907163 28.414667702600948 22.312027367185845 58-59 20.527183435202257 26.91360099924666 30.007258345027175 22.5519572205239 60-61 21.951714873617476 27.04308181797737 28.65586856670092 22.349334741704226 62-63 21.74987335821704 27.101244083204 28.429513787454013 22.719368771124948 64-65 21.094490234473863 26.945523135108495 28.877572804473484 23.082413825944155 66-67 21.311343199892093 26.9170193548296 29.03682057358995 22.734816871688363 68-69 21.914149444471402 26.175747330418396 29.34124485867502 22.568858366435187 70-71 20.989552887740594 26.628576130721733 29.544163543390777 22.837707438146897 72-73 20.856565575176283 26.469754192301043 29.61665222786235 23.057028004660328 74-75 21.300984559668144 26.52244506645887 28.942209754901825 23.234360618971152 76-77 19.64343300888622 26.97599500697497 29.73190373443235 23.648668249706454 78-79 20.493599049386752 27.1848066319201 28.536365459224893 23.78522885946825 80-81 20.819275585148112 27.241876071997577 28.04618581207811 23.8926625307762 82-83 21.127032773622922 27.16658613780322 27.65479991478329 24.05158117379057 84-85 21.04047860567735 26.861137934707614 27.736488391667987 24.36189506794705 86-87 20.949856338924842 26.76613168917965 28.003883875844082 24.280128096051428 88-89 20.901519389982703 26.794443319671757 27.771566000129777 24.532471290215767 90-91 20.61623746060473 26.89969195523505 28.023016343412483 24.461054240747735 92-93 20.44259042594443 26.664111323684402 28.238590365687433 24.65470788468373 94-95 19.363960737376722 26.59851590838721 28.95430151702911 25.08322183720696 96-97 19.155194249283184 26.510651097261217 29.20751233637113 25.126642317084467 98-99 19.28172662825596 26.421215603378066 28.748443716651444 25.548614051714523 100-101 19.110462152671076 26.448056093273298 28.909560613690743 25.531921140364883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 810.0 1 598.5 2 417.5 3 543.5 4 643.5 5 730.5 6 1023.5 7 1632.5 8 2270.5 9 2861.0 10 3266.5 11 3543.5 12 3953.5 13 4366.5 14 4808.0 15 5173.5 16 5481.5 17 5800.0 18 6177.0 19 6752.0 20 7451.5 21 8364.5 22 9708.0 23 11473.0 24 13739.5 25 16737.5 26 20547.0 27 25137.0 28 30011.5 29 35372.5 30 40995.5 31 47071.5 32 54244.0 33 61523.0 34 69318.0 35 77249.5 36 85281.0 37 92793.0 38 98814.0 39 104238.0 40 109856.0 41 117206.5 42 125060.0 43 132352.0 44 138687.0 45 143424.0 46 147225.5 47 149758.5 48 152116.0 49 152219.0 50 148120.0 51 141829.0 52 133976.5 53 125497.0 54 115849.5 55 104360.5 56 92098.0 57 79630.5 58 67921.5 59 56915.0 60 46165.5 61 36126.5 62 27612.5 63 20816.0 64 15526.5 65 11532.5 66 8365.5 67 5974.5 68 4080.0 69 2724.0 70 1928.0 71 1475.0 72 1179.0 73 940.5 74 697.5 75 451.5 76 281.5 77 162.5 78 81.0 79 44.0 80 27.0 81 15.5 82 10.0 83 5.5 84 1.5 85 1.5 86 1.0 87 1.5 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021053511007143155 2 0.005151983513652756 3 0.003648161298856817 4 0.0 5 0.06730996839188493 6 2.784855953325814E-5 7 0.0 8 1.3924279766629072E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0886419649943606E-4 16-17 0.0 18-19 0.00135065513736302 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.746995836640349E-5 28-29 0.0022557333221939094 30-31 0.008730523413676427 32-33 3.8987983346561395E-4 34-35 0.0011000181015636966 36-37 0.025606750490830863 38-39 0.024548505228567052 40-41 0.026609298634028153 42-43 0.02726373978305972 44-45 0.028071348009524207 46-47 0.029505548825487005 48-49 0.02925491178968768 50-51 0.024381413871367504 52-53 0.02096996532854338 54-55 0.016193937368589612 56-57 0.15819374242867287 58-59 0.7620897559073757 60-61 0.31470264700558365 62-63 3.620312739323559E-4 64-65 0.14198588078031663 66-67 1.4140802317000152 68-69 2.7142737791887717 70-71 2.193881671470543 72-73 1.8436999596195887 74-75 1.5572775248200288 76-77 1.1222134035117035 78-79 0.41920436665413485 80-81 1.9493991673280697E-4 82-83 1.3924279766629072E-4 84-85 6.962139883314536E-5 86-87 0.0029797958700586214 88-89 2.784855953325814E-5 90-91 0.22533661946335823 92-93 0.1727724633443335 94-95 1.2882047426096885 96-97 1.2569586588133728 98-99 0.9887491819485638 100-101 0.40438893298244144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3590850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.35416158123077 #Duplication Level Percentage of deduplicated Percentage of total 1 67.88389702347376 30.10933337342786 2 15.329865204022841 13.598866365554327 3 6.379583828137052 8.488832758025927 4 3.4085290805078614 6.047297983646785 5 1.9922698741298976 4.418272995528789 6 1.2124594133084203 3.226657243713956 7 0.8137264331445331 2.5264507589027843 8 0.5441826388222046 1.9309411753616483 9 0.411011951981033 1.6407081476985421 >10 1.818609559953222 13.873277711909246 >50 0.11932661207942517 3.702742896648794 >100 0.08023673145636322 6.8352608531956545 >500 0.0040330553554285935 1.2337322334683958 >1k 0.00207954415892387 1.3095897118771471 >5k 6.301648966435971E-5 0.25144211909435304 >10k+ 1.2603297932871943E-4 0.806593671945847 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15866 0.44184524555467364 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12992 0.3618084854560898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8996 0.2505256415611902 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.784855953325814E-5 9 0.0 0.0 0.0 0.0 2.784855953325814E-5 10-11 0.0 0.0 0.0 1.392427976662907E-5 2.784855953325814E-5 12-13 0.0 0.0 0.0 8.354567859977442E-5 2.784855953325814E-5 14-15 0.0 0.0 0.0 1.1139423813303256E-4 2.784855953325814E-5 16-17 0.0 0.0 0.0 1.5316707743291978E-4 2.784855953325814E-5 18-19 0.0 0.0 0.0 2.2278847626606513E-4 2.784855953325814E-5 20-21 0.0 0.0 0.0 4.177283929988721E-4 2.784855953325814E-5 22-23 0.0 0.0 0.0 8.911539050642605E-4 2.784855953325814E-5 24-25 0.0 0.0 0.0 0.0018101563696617793 2.784855953325814E-5 26-27 0.0 0.0 0.0 0.004051965412089059 2.784855953325814E-5 28-29 0.0 0.0 0.0 0.010512831223804949 2.784855953325814E-5 30-31 0.0 0.0 0.0 0.03236002617764596 2.784855953325814E-5 32-33 0.0 0.0 0.0 0.0789367419970202 2.784855953325814E-5 34-35 0.0 0.0 0.0 0.1484328223122659 2.784855953325814E-5 36-37 0.0 0.0 0.0 0.2658841221437821 2.784855953325814E-5 38-39 0.0 0.0 0.0 0.44201233691187325 2.784855953325814E-5 40-41 0.0 0.0 0.0 0.6348079145606194 2.784855953325814E-5 42-43 0.0 0.0 0.0 0.8276452650486654 2.784855953325814E-5 44-45 0.0 0.0 0.0 1.055335087792584 2.784855953325814E-5 46-47 0.0 0.0 0.0 1.320397677430135 2.784855953325814E-5 48-49 0.0 0.0 0.0 1.585390645668853 2.784855953325814E-5 50-51 0.0 0.0 0.0 1.844215157970954 2.784855953325814E-5 52-53 0.0 2.784855953325814E-5 0.0 2.0900761658103235 2.784855953325814E-5 54-55 0.0 2.784855953325814E-5 0.0 2.3556678780790063 2.784855953325814E-5 56-57 0.0 4.177283929988721E-5 0.0 2.5912806160101365 2.784855953325814E-5 58-59 0.0 5.569711906651628E-5 0.0 2.7599175682637815 2.784855953325814E-5 60-61 0.0 5.569711906651628E-5 0.0 2.8873247281284375 4.177283929988721E-5 62-63 4.177283929988721E-5 5.569711906651628E-5 2.784855953325814E-5 2.9942492724563823 5.569711906651628E-5 64-65 6.962139883314535E-5 5.569711906651628E-5 5.569711906651628E-5 3.105740980547781 5.569711906651628E-5 66-67 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 3.20009190024646 5.569711906651628E-5 68-69 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 3.306682261860005 5.569711906651628E-5 70-71 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 3.5021095283846444 5.569711906651628E-5 72-73 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 3.7439324950916912 5.569711906651628E-5 74-75 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 4.021290223763176 5.569711906651628E-5 76-77 8.354567859977442E-5 5.569711906651628E-5 5.569711906651628E-5 4.417937257195371 5.569711906651628E-5 78-79 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 4.965537407577592 5.569711906651628E-5 80-81 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 5.576910759290976 5.569711906651628E-5 82-83 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 6.158806410738405 5.569711906651628E-5 84-85 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 6.635977554061016 5.569711906651628E-5 86-87 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 7.053538855702689 5.569711906651628E-5 88-89 8.354567859977442E-5 8.354567859977442E-5 5.569711906651628E-5 7.485010512831224 5.569711906651628E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9860 0.0 44.11389 1 GTATCAA 16100 0.0 36.978973 1 ATCAACG 20610 0.0 28.627249 3 TCAACGC 20670 0.0 28.543356 4 TATCAAC 20850 0.0 28.25288 2 CAACGCA 21145 0.0 27.902159 5 AACGCAG 21595 0.0 27.302853 6 GTACATG 17650 0.0 25.552567 1 TACATGG 17775 0.0 24.702816 2 ACGCAGA 24570 0.0 23.939404 7 ACATGGG 17730 0.0 23.834146 3 CGCAGAG 24635 0.0 23.74331 8 GCAGAGT 26015 0.0 21.795351 9 CATGGGA 11485 0.0 21.5584 4 GAGTACT 13445 0.0 21.5068 12-13 AGTACTT 14130 0.0 19.897125 12-13 AGAGTAC 23380 0.0 19.857006 10-11 GTACTTT 14945 0.0 19.55279 14-15 CAGAGTA 25770 0.0 18.82929 10-11 CATGGGG 6320 0.0 18.730305 4 >>END_MODULE