FastQCFastQC Report
Fri 27 May 2016
SRR939844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811259
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148550.3897662163605255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114320.29995337498711055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78200.20518154237221872No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39780.10437495851108518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97400.044.9658851
GTATCAA164700.038.673861
ATCAACG210300.030.0671583
TATCAAC211100.029.9512422
TCAACGC213250.029.739474
CAACGCA218100.028.8855175
AACGCAG224400.028.2003576
GTACATG182650.027.738851
TACATGG182400.027.3649352
ACATGGG186000.026.078233
ACGCAGA250950.025.048087
CGCAGAG251850.024.8271478
CATGGGA120300.023.1911054
GCAGAGT260250.022.9049669
GAGTACT138300.021.94110512-13
AGTACTT143600.021.06577712-13
CATGGGG70350.020.7314624
AGAGTAC232850.020.30589710-11
GTACTTT153200.020.24278614-15
ATGGGAG51550.019.6229825
Produced by FastQC (version 0.11.5)